Comparative study of the cell wall composition of broccoli, carrot, and tomato: Structural characterization of the extractable pectins and hemicelluloses
[Display omitted] ► Occurrence of a hemicellulosic mannan is inferred for both broccoli organs. ► Broccoli organs show similar molar-mass distributions of pectin and hemicellulose. ► Polysaccharides in HF of carrot show deviating size-distribution profile. ► Tomato fruit possesses very long, linear...
Gespeichert in:
| Veröffentlicht in: | Carbohydrate research 2011-07, Vol.346 (9), p.1105-1111 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1111 |
|---|---|
| container_issue | 9 |
| container_start_page | 1105 |
| container_title | Carbohydrate research |
| container_volume | 346 |
| creator | Houben, Ken Jolie, Ruben P. Fraeye, Ilse Van Loey, Ann M. Hendrickx, Marc E. |
| description | [Display omitted]
► Occurrence of a hemicellulosic mannan is inferred for both broccoli organs. ► Broccoli organs show similar molar-mass distributions of pectin and hemicellulose. ► Polysaccharides in HF of carrot show deviating size-distribution profile. ► Tomato fruit possesses very long, linear pectins with a high range in DM.
This study delivers a comparison of the pectic and hemicellulosic cell wall polysaccharides between the commonly used vegetables broccoli (stem and florets separately), carrot, and tomato. Alcohol-insoluble residues were prepared from the plant sources and sequentially extracted with water, cyclohexane-
trans-1,2-diamine tetra-acetic acid, sodium carbonate, and potassium hydroxide solutions, to obtain individual fractions, each containing polysaccharides bound to the cell wall in a specific manner. Structural characterization of the polysaccharide fractions was conducted using colorimetric and chromatographic approaches. Sugar ratios were defined to ameliorate data interpretation. These ratios allowed gaining information concerning polysaccharide structure from sugar composition data. Structural analysis of broccoli revealed organ-specific characteristics: the pectin degree of methoxylation (DM) of stem and florets differed, the sugar composition data inferred differences in polymeric composition. On the other hand, the molar mass (MM) distribution profiles of the polysaccharide fractions were virtually identical for both organs. Carrot root displayed a different MM distribution for the polysaccharides solubilized by potassium hydroxide compared to broccoli and tomato, possibly due to the high contribution of branched pectins to this otherwise hemicellulose-enriched fraction. Tomato fruit showed the pectins with the broadest range in DM, the highest MM, the greatest overall linearity and the lowest extent of branching of rhamnogalacturonan I, pointing to particularly long, linear pectins in tomato compared with the other vegetable organs studied, suggesting possible implications toward functional behavior. |
| doi_str_mv | 10.1016/j.carres.2011.04.014 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_867321931</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0008621511001947</els_id><sourcerecordid>867321931</sourcerecordid><originalsourceid>FETCH-LOGICAL-c385t-f95bed9f5df3a1323210afe19585c2bc9c9c805315253f4214019ec76956d5f33</originalsourceid><addsrcrecordid>eNp9kc1u1DAUhS0EokPhDRB4x6YJ_ok9CYtKaFR-pEosSiV2luNcMx4l8WA7hfZNeFts0rJElmz5-jvnXvkg9JKSmhIq3x5qo0OAWDNCaU2amtDmEdrQdsurhslvj9GGENJWklFxgp7FeMhXIrfyKTrJJS6ZJBv0e-enow46uRvAMS3DLfYWpz1gA-OIf-q8mYz46JLzc3nsgzfGj-4MlwF8OsN6HnDyk07-Hb5KYTFpCTrr9tnYJAjuTj-IizP8SqWu-xHwEUxyc_xrsYfJla7L6CPE5-iJ1WOEF_fnKbr-cPF196m6_PLx8-79ZWV4K1JlO9HD0FkxWK4pZ5xRoi3QTrTCsN50ebVEcCqY4LZhtCG0A7OVnZCDsJyfojer7zH4HwvEpCYXyxh6Br9E1cpt9uw4zWSzkib4GANYdQxu0uFWUaJKJuqg1kxUyUSRRuVMsuzVfYOln2D4J3oIIQOvV8Bqr_T34KK6vsoOsgTWENll4nwlIH_EjYOgonEwGxhcyB-oBu_-P8MfEeGrjA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>867321931</pqid></control><display><type>article</type><title>Comparative study of the cell wall composition of broccoli, carrot, and tomato: Structural characterization of the extractable pectins and hemicelluloses</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Houben, Ken ; Jolie, Ruben P. ; Fraeye, Ilse ; Van Loey, Ann M. ; Hendrickx, Marc E.</creator><creatorcontrib>Houben, Ken ; Jolie, Ruben P. ; Fraeye, Ilse ; Van Loey, Ann M. ; Hendrickx, Marc E.</creatorcontrib><description>[Display omitted]
► Occurrence of a hemicellulosic mannan is inferred for both broccoli organs. ► Broccoli organs show similar molar-mass distributions of pectin and hemicellulose. ► Polysaccharides in HF of carrot show deviating size-distribution profile. ► Tomato fruit possesses very long, linear pectins with a high range in DM.
This study delivers a comparison of the pectic and hemicellulosic cell wall polysaccharides between the commonly used vegetables broccoli (stem and florets separately), carrot, and tomato. Alcohol-insoluble residues were prepared from the plant sources and sequentially extracted with water, cyclohexane-
trans-1,2-diamine tetra-acetic acid, sodium carbonate, and potassium hydroxide solutions, to obtain individual fractions, each containing polysaccharides bound to the cell wall in a specific manner. Structural characterization of the polysaccharide fractions was conducted using colorimetric and chromatographic approaches. Sugar ratios were defined to ameliorate data interpretation. These ratios allowed gaining information concerning polysaccharide structure from sugar composition data. Structural analysis of broccoli revealed organ-specific characteristics: the pectin degree of methoxylation (DM) of stem and florets differed, the sugar composition data inferred differences in polymeric composition. On the other hand, the molar mass (MM) distribution profiles of the polysaccharide fractions were virtually identical for both organs. Carrot root displayed a different MM distribution for the polysaccharides solubilized by potassium hydroxide compared to broccoli and tomato, possibly due to the high contribution of branched pectins to this otherwise hemicellulose-enriched fraction. Tomato fruit showed the pectins with the broadest range in DM, the highest MM, the greatest overall linearity and the lowest extent of branching of rhamnogalacturonan I, pointing to particularly long, linear pectins in tomato compared with the other vegetable organs studied, suggesting possible implications toward functional behavior.</description><identifier>ISSN: 0008-6215</identifier><identifier>EISSN: 1873-426X</identifier><identifier>DOI: 10.1016/j.carres.2011.04.014</identifier><identifier>PMID: 21536260</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Brassica - chemistry ; Brassica - cytology ; Broccoli ; Carbohydrate Conformation ; Carrot ; carrots ; Cell Wall - chemistry ; cell wall components ; cell walls ; Chemical Fractionation ; chromatography ; colorimetry ; Daucus carota - chemistry ; Daucus carota - cytology ; florets ; Hemicellulose ; Lycopersicon esculentum - chemistry ; Lycopersicon esculentum - cytology ; molecular weight ; Pectin ; pectins ; Pectins - chemistry ; Pectins - isolation & purification ; Polysaccharides - chemistry ; Polysaccharides - isolation & purification ; potassium hydroxide ; sodium carbonate ; solubilization ; Structure ; sugars ; Tomato ; tomatoes</subject><ispartof>Carbohydrate research, 2011-07, Vol.346 (9), p.1105-1111</ispartof><rights>2011 Elsevier Ltd</rights><rights>Copyright © 2011 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-f95bed9f5df3a1323210afe19585c2bc9c9c805315253f4214019ec76956d5f33</citedby><cites>FETCH-LOGICAL-c385t-f95bed9f5df3a1323210afe19585c2bc9c9c805315253f4214019ec76956d5f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.carres.2011.04.014$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21536260$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Houben, Ken</creatorcontrib><creatorcontrib>Jolie, Ruben P.</creatorcontrib><creatorcontrib>Fraeye, Ilse</creatorcontrib><creatorcontrib>Van Loey, Ann M.</creatorcontrib><creatorcontrib>Hendrickx, Marc E.</creatorcontrib><title>Comparative study of the cell wall composition of broccoli, carrot, and tomato: Structural characterization of the extractable pectins and hemicelluloses</title><title>Carbohydrate research</title><addtitle>Carbohydr Res</addtitle><description>[Display omitted]
► Occurrence of a hemicellulosic mannan is inferred for both broccoli organs. ► Broccoli organs show similar molar-mass distributions of pectin and hemicellulose. ► Polysaccharides in HF of carrot show deviating size-distribution profile. ► Tomato fruit possesses very long, linear pectins with a high range in DM.
This study delivers a comparison of the pectic and hemicellulosic cell wall polysaccharides between the commonly used vegetables broccoli (stem and florets separately), carrot, and tomato. Alcohol-insoluble residues were prepared from the plant sources and sequentially extracted with water, cyclohexane-
trans-1,2-diamine tetra-acetic acid, sodium carbonate, and potassium hydroxide solutions, to obtain individual fractions, each containing polysaccharides bound to the cell wall in a specific manner. Structural characterization of the polysaccharide fractions was conducted using colorimetric and chromatographic approaches. Sugar ratios were defined to ameliorate data interpretation. These ratios allowed gaining information concerning polysaccharide structure from sugar composition data. Structural analysis of broccoli revealed organ-specific characteristics: the pectin degree of methoxylation (DM) of stem and florets differed, the sugar composition data inferred differences in polymeric composition. On the other hand, the molar mass (MM) distribution profiles of the polysaccharide fractions were virtually identical for both organs. Carrot root displayed a different MM distribution for the polysaccharides solubilized by potassium hydroxide compared to broccoli and tomato, possibly due to the high contribution of branched pectins to this otherwise hemicellulose-enriched fraction. Tomato fruit showed the pectins with the broadest range in DM, the highest MM, the greatest overall linearity and the lowest extent of branching of rhamnogalacturonan I, pointing to particularly long, linear pectins in tomato compared with the other vegetable organs studied, suggesting possible implications toward functional behavior.</description><subject>Brassica - chemistry</subject><subject>Brassica - cytology</subject><subject>Broccoli</subject><subject>Carbohydrate Conformation</subject><subject>Carrot</subject><subject>carrots</subject><subject>Cell Wall - chemistry</subject><subject>cell wall components</subject><subject>cell walls</subject><subject>Chemical Fractionation</subject><subject>chromatography</subject><subject>colorimetry</subject><subject>Daucus carota - chemistry</subject><subject>Daucus carota - cytology</subject><subject>florets</subject><subject>Hemicellulose</subject><subject>Lycopersicon esculentum - chemistry</subject><subject>Lycopersicon esculentum - cytology</subject><subject>molecular weight</subject><subject>Pectin</subject><subject>pectins</subject><subject>Pectins - chemistry</subject><subject>Pectins - isolation & purification</subject><subject>Polysaccharides - chemistry</subject><subject>Polysaccharides - isolation & purification</subject><subject>potassium hydroxide</subject><subject>sodium carbonate</subject><subject>solubilization</subject><subject>Structure</subject><subject>sugars</subject><subject>Tomato</subject><subject>tomatoes</subject><issn>0008-6215</issn><issn>1873-426X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1DAUhS0EokPhDRB4x6YJ_ok9CYtKaFR-pEosSiV2luNcMx4l8WA7hfZNeFts0rJElmz5-jvnXvkg9JKSmhIq3x5qo0OAWDNCaU2amtDmEdrQdsurhslvj9GGENJWklFxgp7FeMhXIrfyKTrJJS6ZJBv0e-enow46uRvAMS3DLfYWpz1gA-OIf-q8mYz46JLzc3nsgzfGj-4MlwF8OsN6HnDyk07-Hb5KYTFpCTrr9tnYJAjuTj-IizP8SqWu-xHwEUxyc_xrsYfJla7L6CPE5-iJ1WOEF_fnKbr-cPF196m6_PLx8-79ZWV4K1JlO9HD0FkxWK4pZ5xRoi3QTrTCsN50ebVEcCqY4LZhtCG0A7OVnZCDsJyfojer7zH4HwvEpCYXyxh6Br9E1cpt9uw4zWSzkib4GANYdQxu0uFWUaJKJuqg1kxUyUSRRuVMsuzVfYOln2D4J3oIIQOvV8Bqr_T34KK6vsoOsgTWENll4nwlIH_EjYOgonEwGxhcyB-oBu_-P8MfEeGrjA</recordid><startdate>20110701</startdate><enddate>20110701</enddate><creator>Houben, Ken</creator><creator>Jolie, Ruben P.</creator><creator>Fraeye, Ilse</creator><creator>Van Loey, Ann M.</creator><creator>Hendrickx, Marc E.</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110701</creationdate><title>Comparative study of the cell wall composition of broccoli, carrot, and tomato: Structural characterization of the extractable pectins and hemicelluloses</title><author>Houben, Ken ; Jolie, Ruben P. ; Fraeye, Ilse ; Van Loey, Ann M. ; Hendrickx, Marc E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-f95bed9f5df3a1323210afe19585c2bc9c9c805315253f4214019ec76956d5f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Brassica - chemistry</topic><topic>Brassica - cytology</topic><topic>Broccoli</topic><topic>Carbohydrate Conformation</topic><topic>Carrot</topic><topic>carrots</topic><topic>Cell Wall - chemistry</topic><topic>cell wall components</topic><topic>cell walls</topic><topic>Chemical Fractionation</topic><topic>chromatography</topic><topic>colorimetry</topic><topic>Daucus carota - chemistry</topic><topic>Daucus carota - cytology</topic><topic>florets</topic><topic>Hemicellulose</topic><topic>Lycopersicon esculentum - chemistry</topic><topic>Lycopersicon esculentum - cytology</topic><topic>molecular weight</topic><topic>Pectin</topic><topic>pectins</topic><topic>Pectins - chemistry</topic><topic>Pectins - isolation & purification</topic><topic>Polysaccharides - chemistry</topic><topic>Polysaccharides - isolation & purification</topic><topic>potassium hydroxide</topic><topic>sodium carbonate</topic><topic>solubilization</topic><topic>Structure</topic><topic>sugars</topic><topic>Tomato</topic><topic>tomatoes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Houben, Ken</creatorcontrib><creatorcontrib>Jolie, Ruben P.</creatorcontrib><creatorcontrib>Fraeye, Ilse</creatorcontrib><creatorcontrib>Van Loey, Ann M.</creatorcontrib><creatorcontrib>Hendrickx, Marc E.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Houben, Ken</au><au>Jolie, Ruben P.</au><au>Fraeye, Ilse</au><au>Van Loey, Ann M.</au><au>Hendrickx, Marc E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative study of the cell wall composition of broccoli, carrot, and tomato: Structural characterization of the extractable pectins and hemicelluloses</atitle><jtitle>Carbohydrate research</jtitle><addtitle>Carbohydr Res</addtitle><date>2011-07-01</date><risdate>2011</risdate><volume>346</volume><issue>9</issue><spage>1105</spage><epage>1111</epage><pages>1105-1111</pages><issn>0008-6215</issn><eissn>1873-426X</eissn><abstract>[Display omitted]
► Occurrence of a hemicellulosic mannan is inferred for both broccoli organs. ► Broccoli organs show similar molar-mass distributions of pectin and hemicellulose. ► Polysaccharides in HF of carrot show deviating size-distribution profile. ► Tomato fruit possesses very long, linear pectins with a high range in DM.
This study delivers a comparison of the pectic and hemicellulosic cell wall polysaccharides between the commonly used vegetables broccoli (stem and florets separately), carrot, and tomato. Alcohol-insoluble residues were prepared from the plant sources and sequentially extracted with water, cyclohexane-
trans-1,2-diamine tetra-acetic acid, sodium carbonate, and potassium hydroxide solutions, to obtain individual fractions, each containing polysaccharides bound to the cell wall in a specific manner. Structural characterization of the polysaccharide fractions was conducted using colorimetric and chromatographic approaches. Sugar ratios were defined to ameliorate data interpretation. These ratios allowed gaining information concerning polysaccharide structure from sugar composition data. Structural analysis of broccoli revealed organ-specific characteristics: the pectin degree of methoxylation (DM) of stem and florets differed, the sugar composition data inferred differences in polymeric composition. On the other hand, the molar mass (MM) distribution profiles of the polysaccharide fractions were virtually identical for both organs. Carrot root displayed a different MM distribution for the polysaccharides solubilized by potassium hydroxide compared to broccoli and tomato, possibly due to the high contribution of branched pectins to this otherwise hemicellulose-enriched fraction. Tomato fruit showed the pectins with the broadest range in DM, the highest MM, the greatest overall linearity and the lowest extent of branching of rhamnogalacturonan I, pointing to particularly long, linear pectins in tomato compared with the other vegetable organs studied, suggesting possible implications toward functional behavior.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>21536260</pmid><doi>10.1016/j.carres.2011.04.014</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0008-6215 |
| ispartof | Carbohydrate research, 2011-07, Vol.346 (9), p.1105-1111 |
| issn | 0008-6215 1873-426X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_867321931 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Brassica - chemistry Brassica - cytology Broccoli Carbohydrate Conformation Carrot carrots Cell Wall - chemistry cell wall components cell walls Chemical Fractionation chromatography colorimetry Daucus carota - chemistry Daucus carota - cytology florets Hemicellulose Lycopersicon esculentum - chemistry Lycopersicon esculentum - cytology molecular weight Pectin pectins Pectins - chemistry Pectins - isolation & purification Polysaccharides - chemistry Polysaccharides - isolation & purification potassium hydroxide sodium carbonate solubilization Structure sugars Tomato tomatoes |
| title | Comparative study of the cell wall composition of broccoli, carrot, and tomato: Structural characterization of the extractable pectins and hemicelluloses |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T05%3A24%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20study%20of%20the%20cell%20wall%20composition%20of%20broccoli,%20carrot,%20and%20tomato:%20Structural%20characterization%20of%20the%20extractable%20pectins%20and%20hemicelluloses&rft.jtitle=Carbohydrate%20research&rft.au=Houben,%20Ken&rft.date=2011-07-01&rft.volume=346&rft.issue=9&rft.spage=1105&rft.epage=1111&rft.pages=1105-1111&rft.issn=0008-6215&rft.eissn=1873-426X&rft_id=info:doi/10.1016/j.carres.2011.04.014&rft_dat=%3Cproquest_cross%3E867321931%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=867321931&rft_id=info:pmid/21536260&rft_els_id=S0008621511001947&rfr_iscdi=true |