Mucoadhesive polyelectrolyte microparticles containing recombinant human insulin and its analogs aspart and lispro
Microparticles containing recombinant human insulin and its analogs aspart and lispro were prepared using an alternate adsorption of chitosan and dextran sulfate from solutions onto microaggregates of protein-dextran sulfate insoluble complex. The following properties of polyelectrolyte hormone-cont...
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| Veröffentlicht in: | Biochemistry (Moscow) 2011-03, Vol.76 (3), p.327-331 |
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| creator | Balabushevich, N. G. Pechenkin, M. A. Zorov, I. N. Shibanova, E. D. Larionova, N. I. |
| description | Microparticles containing recombinant human insulin and its analogs aspart and lispro were prepared using an alternate adsorption of chitosan and dextran sulfate from solutions onto microaggregates of protein-dextran sulfate insoluble complex. The following properties of polyelectrolyte hormone-containing microparticles were studied: pH stability, surface charge, mucoadhesive properties, Ca
2+
binding, degradation under the influence of proteases (trypsin, chymotrypsin). The influence of the self-association ability of encapsulated insulins on the form of protein releasing from microparticles was studied. Insulins aspart and lispro released from the microparticles as monomers were more liable to proteolysis than human insulin released as a hexamer. The combined effect of properties of polyelectrolyte microparticles and of encapsulated recombinant proteins on the bioavailability of insulin under peroral administration is discussed. |
| doi_str_mv | 10.1134/S0006297911030059 |
| format | Article |
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2+
binding, degradation under the influence of proteases (trypsin, chymotrypsin). The influence of the self-association ability of encapsulated insulins on the form of protein releasing from microparticles was studied. Insulins aspart and lispro released from the microparticles as monomers were more liable to proteolysis than human insulin released as a hexamer. The combined effect of properties of polyelectrolyte microparticles and of encapsulated recombinant proteins on the bioavailability of insulin under peroral administration is discussed.</description><identifier>ISSN: 0006-2979</identifier><identifier>EISSN: 1608-3040</identifier><identifier>DOI: 10.1134/S0006297911030059</identifier><identifier>PMID: 21568867</identifier><language>eng</language><publisher>Dordrecht: SP MAIK Nauka/Interperiodica</publisher><subject>Adsorption ; Atoms & subatomic particles ; Bioavailability ; Biochemistry ; Biological Availability ; Biomedical and Life Sciences ; Biomedicine ; Bioorganic Chemistry ; Chemical Phenomena ; Chitosan - chemistry ; Dextran ; Dextran Sulfate - chemistry ; Electrolytes ; Electrolytes - chemistry ; Enzymes ; Humans ; Hydrogen-Ion Concentration ; Hydrolases ; Insulin ; Insulin - analogs & derivatives ; Insulin - chemistry ; Insulin - metabolism ; Insulin Aspart ; Insulin Lispro ; Kinetics ; Life Sciences ; Microbiology ; Mucous Membrane - metabolism ; Nanostructures - chemistry ; Polyelectrolytes ; Polymers - chemistry ; Protein Multimerization ; Protein Structure, Quaternary ; Proteolysis ; Recombinant proteins ; Recombinant Proteins - chemistry ; Recombinant Proteins - metabolism ; Sulfates</subject><ispartof>Biochemistry (Moscow), 2011-03, Vol.76 (3), p.327-331</ispartof><rights>Pleiades Publishing, Ltd. 2011</rights><rights>COPYRIGHT 2011 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c437t-2e8c3447f1e1d235464a06a5782a6b7315f9f5c50d72fdc634aa3f9181116eea3</citedby><cites>FETCH-LOGICAL-c437t-2e8c3447f1e1d235464a06a5782a6b7315f9f5c50d72fdc634aa3f9181116eea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0006297911030059$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0006297911030059$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21568867$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Balabushevich, N. G.</creatorcontrib><creatorcontrib>Pechenkin, M. A.</creatorcontrib><creatorcontrib>Zorov, I. N.</creatorcontrib><creatorcontrib>Shibanova, E. D.</creatorcontrib><creatorcontrib>Larionova, N. I.</creatorcontrib><title>Mucoadhesive polyelectrolyte microparticles containing recombinant human insulin and its analogs aspart and lispro</title><title>Biochemistry (Moscow)</title><addtitle>Biochemistry Moscow</addtitle><addtitle>Biochemistry (Mosc)</addtitle><description>Microparticles containing recombinant human insulin and its analogs aspart and lispro were prepared using an alternate adsorption of chitosan and dextran sulfate from solutions onto microaggregates of protein-dextran sulfate insoluble complex. The following properties of polyelectrolyte hormone-containing microparticles were studied: pH stability, surface charge, mucoadhesive properties, Ca
2+
binding, degradation under the influence of proteases (trypsin, chymotrypsin). The influence of the self-association ability of encapsulated insulins on the form of protein releasing from microparticles was studied. Insulins aspart and lispro released from the microparticles as monomers were more liable to proteolysis than human insulin released as a hexamer. The combined effect of properties of polyelectrolyte microparticles and of encapsulated recombinant proteins on the bioavailability of insulin under peroral administration is discussed.</description><subject>Adsorption</subject><subject>Atoms & subatomic particles</subject><subject>Bioavailability</subject><subject>Biochemistry</subject><subject>Biological Availability</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bioorganic Chemistry</subject><subject>Chemical Phenomena</subject><subject>Chitosan - chemistry</subject><subject>Dextran</subject><subject>Dextran Sulfate - chemistry</subject><subject>Electrolytes</subject><subject>Electrolytes - chemistry</subject><subject>Enzymes</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydrolases</subject><subject>Insulin</subject><subject>Insulin - analogs & derivatives</subject><subject>Insulin - chemistry</subject><subject>Insulin - metabolism</subject><subject>Insulin Aspart</subject><subject>Insulin Lispro</subject><subject>Kinetics</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Mucous Membrane - metabolism</subject><subject>Nanostructures - chemistry</subject><subject>Polyelectrolytes</subject><subject>Polymers - chemistry</subject><subject>Protein Multimerization</subject><subject>Protein Structure, Quaternary</subject><subject>Proteolysis</subject><subject>Recombinant proteins</subject><subject>Recombinant Proteins - chemistry</subject><subject>Recombinant Proteins - metabolism</subject><subject>Sulfates</subject><issn>0006-2979</issn><issn>1608-3040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kUtv1DAQxy1ERZfCB-CCIjhwSvHbybGqeEmtOADnyOuMt64ce7GTSv32TLoFBBT5MPbM7z-eByEvGD1lTMi3XyilmvemZ4wKSlX_iGyYpl0rqKSPyWYNt2v8mDyt9RqfnPbiCTnmTOmu02ZDyuXish2voIYbaPY53kIENxe8zNBMwZW8t2UOLkJtXE6zDSmkXVPA5Wkbkk1zc7VMNjUh1SWG1Ng0NmGuaG3MO7R1TXDnjqHuS35GjryNFZ7f2xPy7f27r-cf24vPHz6dn120Tgoztxw6J6Q0ngEbuVBSS0u1VabjVm-NYMr3XjlFR8P96LSQ1grfs44xpgGsOCFvDnnxz-8L1HmYQnUQo02Qlzpg_4LTznAkX_1FXuelYP0IKWNUp5RC6PUB2tkIQ0g-z8W6NeVwJqTSvUQSqdMHKDwj4DBzAh_Q_4eAHQQ46VoL-GFfwmTL7cDosG55-GfLqHl5X--ynWD8pfi5VgT4AcB547ag_G7o_1l_AA6ysUU</recordid><startdate>20110301</startdate><enddate>20110301</enddate><creator>Balabushevich, N. 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G.</au><au>Pechenkin, M. A.</au><au>Zorov, I. N.</au><au>Shibanova, E. D.</au><au>Larionova, N. I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mucoadhesive polyelectrolyte microparticles containing recombinant human insulin and its analogs aspart and lispro</atitle><jtitle>Biochemistry (Moscow)</jtitle><stitle>Biochemistry Moscow</stitle><addtitle>Biochemistry (Mosc)</addtitle><date>2011-03-01</date><risdate>2011</risdate><volume>76</volume><issue>3</issue><spage>327</spage><epage>331</epage><pages>327-331</pages><issn>0006-2979</issn><eissn>1608-3040</eissn><abstract>Microparticles containing recombinant human insulin and its analogs aspart and lispro were prepared using an alternate adsorption of chitosan and dextran sulfate from solutions onto microaggregates of protein-dextran sulfate insoluble complex. The following properties of polyelectrolyte hormone-containing microparticles were studied: pH stability, surface charge, mucoadhesive properties, Ca
2+
binding, degradation under the influence of proteases (trypsin, chymotrypsin). The influence of the self-association ability of encapsulated insulins on the form of protein releasing from microparticles was studied. Insulins aspart and lispro released from the microparticles as monomers were more liable to proteolysis than human insulin released as a hexamer. The combined effect of properties of polyelectrolyte microparticles and of encapsulated recombinant proteins on the bioavailability of insulin under peroral administration is discussed.</abstract><cop>Dordrecht</cop><pub>SP MAIK Nauka/Interperiodica</pub><pmid>21568867</pmid><doi>10.1134/S0006297911030059</doi><tpages>5</tpages></addata></record> |
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| issn | 0006-2979 1608-3040 |
| language | eng |
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| subjects | Adsorption Atoms & subatomic particles Bioavailability Biochemistry Biological Availability Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Chemical Phenomena Chitosan - chemistry Dextran Dextran Sulfate - chemistry Electrolytes Electrolytes - chemistry Enzymes Humans Hydrogen-Ion Concentration Hydrolases Insulin Insulin - analogs & derivatives Insulin - chemistry Insulin - metabolism Insulin Aspart Insulin Lispro Kinetics Life Sciences Microbiology Mucous Membrane - metabolism Nanostructures - chemistry Polyelectrolytes Polymers - chemistry Protein Multimerization Protein Structure, Quaternary Proteolysis Recombinant proteins Recombinant Proteins - chemistry Recombinant Proteins - metabolism Sulfates |
| title | Mucoadhesive polyelectrolyte microparticles containing recombinant human insulin and its analogs aspart and lispro |
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