Development of 14 minisatellite markers for the citrus canker bacterium, Xanthomonas citri pv. citri

We screened the genome of Xanthomonas citri pv. citri strain 306 for tandem repeats. A multiplex polymerase chain reaction protocol was used to assess the genetic diversity of 239 strains of X. citri pv. citri from Asia. The total number of alleles per locus ranged from three to 20. Using pooled dat...

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Veröffentlicht in:Molecular ecology resources 2009, Vol.9 (1), p.125-127
Hauptverfasser: NGOC, L. BUI THI, VERNIERE, C, VITAL, K, GUERIN, F, GAGNEVIN, L, BRISSE, S, AH-YOU, N, PRUVOST, O
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container_end_page 127
container_issue 1
container_start_page 125
container_title Molecular ecology resources
container_volume 9
creator NGOC, L. BUI THI
VERNIERE, C
VITAL, K
GUERIN, F
GAGNEVIN, L
BRISSE, S
AH-YOU, N
PRUVOST, O
description We screened the genome of Xanthomonas citri pv. citri strain 306 for tandem repeats. A multiplex polymerase chain reaction protocol was used to assess the genetic diversity of 239 strains of X. citri pv. citri from Asia. The total number of alleles per locus ranged from three to 20. Using pooled data sets, 223 different haplotypes were identified. Successful amplifications were obtained at most loci for seven other X. citri pathovars. This typing scheme is expected to be useful at different spatial scales for population studies of pathovars of X. citri, several of which cause plant diseases of economic importance.
doi_str_mv 10.1111/j.1755-0998.2008.02242.x
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source Wiley Online Library Journals Frontfile Complete
subjects Asia
Bacteria
Citrus
host range
MLVA
molecular epidemiology
plant pathogenic bacteria
Rutaceae
Xanthomonas
title Development of 14 minisatellite markers for the citrus canker bacterium, Xanthomonas citri pv. citri
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