Pooled samples bias fungal community descriptions

We tested the accuracy of molecular analyses for recovering the species richness and structure of pooled fungal communities of known composition. We constructed replicate pools of 2-20 species and analysed these pools by two separate pooling-DNA extraction procedures and three different molecular an...

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Veröffentlicht in:	Molecular ecology resources 2010, Vol.10 (1), p.135-141
Hauptverfasser:	AVIS, P.G, BRANCO, S, TANG, Y, MUELLER, G.M
Format:	Artikel
Sprache:	eng
Schlagworte:	ARISA clone sequence Deoxyribonucleic acid DNA Ecological studies environmental sample Extraction procedures Fungi Polymorphism pooled samples Restriction fragment length polymorphism Species richness T-RFLP
Online-Zugang:	Volltext
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