Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas

Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas

We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes-Oléron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels ranged fr...

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Veröffentlicht in:Molecular ecology resources 2009-05, Vol.9 (3), p.853-855
Hauptverfasser: SAUVAGE, C, BOUDRY, P, LAPÈGUE, S
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Sprache:eng
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Crassostrea gigas
EST-SSR markers
Marine
null alleles
Pacific oyster
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creator SAUVAGE, C
BOUDRY, P
LAPÈGUE, S
description We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes-Oléron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels ranged from 0.64 to 1 and from 0.77 to 0.97, respectively. The development of these new markers creates a useful complementary tool for population genetics studies, parentage analysis and mapping in Pacific oyster, a species of major aquacultural and ecological importance.
doi_str_mv 10.1111/j.1755-0998.2009.02525.x
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source Wiley Online Library Journals Frontfile Complete
subjects Crassostrea gigas
EST-SSR markers
Marine
null alleles
Pacific oyster
title Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas
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