Effects of Intense Pulsed Light on the Biological Properties and Ultrastructure of Skin Dermal Fibroblasts: Potential Roles in Photoaging
Objectives: The aim of this study was to evaluate the effects of intense pulsed light (IPL) irradiation on skin fibroblasts in vitro, as well as to explore the biomolecular mechanisms and ultrastructure changes of the IPL effect. Background: IPL is frequently used to improve telangiectasias, lentigo...
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| Veröffentlicht in: | Photomedicine and laser surgery 2011-05, Vol.29 (5), p.327-332 |
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| creator | Cao, Yongqian Huo, Ran Feng, Yongqiang Li, Qiang Wang, Fagang |
| description | Objectives:
The aim of this study was to evaluate the effects of intense pulsed light (IPL) irradiation on skin fibroblasts
in vitro,
as well as to explore the biomolecular mechanisms and ultrastructure changes of the IPL effect.
Background:
IPL is frequently used to improve telangiectasias, lentigos, and skin texture. Clinical studies have demonstrated that IPL has significant photorejuvenation effects on photoaged skin. However, the biomolecular mechanisms underlying the photorejuvenation of IPL treatment remain largely unknown.
Methods:
Human skin fibroblasts cultured
in vitro
were irradiated with IPL via double pulses of 4 ms and 6 ms with a pulse interval of 20 ms and fluences of 18, 23, 28, and 33 J/cm
2
. Twenty-four hours after irradiation, cell viability, cell cycle distribution, mRNA and protein levels of procollagen (types I and III), and changes of ultrastructure were evaluated using methyl thiazole tetrazolium (MTT) assay, flow cytometry, real-time reverse transcriptase polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assays (ELISA), and transmission electron microscopy, respectively.
Results:
IPL irradiation resulted in the improvement of cell viability of skin fibroblasts in a dose-dependent manner. There were obvious changes in ultrastructure of fibroblasts, compared with the control group. The percentage of fibroblasts at the S and G
2
/M stage of the cell cycle was significantly higher, whereas the percentage at the G
0
/G
1
stage was lower. IPL irradiation increased the mRNA level of collagen I considerably, to 123%, 154%, 172%, and 141% of the control, and that of collagen III to 120%, 141%, 164%, and 132%. IPL irradiation was able to upregulate expression of collagen (types I and III) at the mRNA and protein level.
Conclusions:
The present study demonstrates that IPL irradiation imparts stimulatory effects on skin fibroblasts
in vitro
. This provides valuable evidence of the photorejuvenation effect of IPL
in vivo
. |
| doi_str_mv | 10.1089/pho.2010.2867 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_866047171</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A257128563</galeid><sourcerecordid>A257128563</sourcerecordid><originalsourceid>FETCH-LOGICAL-c403t-3a68a297cb9b9710ff2fb05739ec2a38926be526f027fca3130624e2fee005c33</originalsourceid><addsrcrecordid>eNqFkU-LFDEQxYMo7rp69CoBD556zJ9Op9vbuu7qwoCDuueQzlR6oulkTNIHP4Lf2jSzCoIgOST1-NWrFA-h55RsKOmH18dD3DBSK9Z38gE6p0LIpheCPFzf7dCIlvZn6EnOXwlhgxz4Y3TGaMt7Seg5-nltLZiScbT4NhQIGfBu8Rn2eOumQ8Ex4HIA_NZFHydntMe7FI-QioOMddjjO1-SziUtpiwJVp_P31zA7yDNFb5xY4qjr0B-g3exDiiuyp-ir-0V2x1iiXpyYXqKHlldBz-7vy_Q3c31l6sPzfbj-9ury21jWsJLw3XX67qHGYdxkJRYy-xIhOQDGKZ5P7BuBME6S5i0RnPKScdaYBaAEGE4v0CvTr7HFL8vkIuaXTbgvQ4Ql6z6riOtpJJW8uWJnLQH5YKNdVOz0uqSCUlZL7rVb_MPqp49zM7EANZV_a-G5tRgUsw5gVXH5GadfihK1JqpqpmqNVO1Zlr5F_cfXsYZ9n_o3yFWgJ-AVdYheAdjDeg_tr8A8ymuKQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>866047171</pqid></control><display><type>article</type><title>Effects of Intense Pulsed Light on the Biological Properties and Ultrastructure of Skin Dermal Fibroblasts: Potential Roles in Photoaging</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Cao, Yongqian ; Huo, Ran ; Feng, Yongqiang ; Li, Qiang ; Wang, Fagang</creator><creatorcontrib>Cao, Yongqian ; Huo, Ran ; Feng, Yongqiang ; Li, Qiang ; Wang, Fagang</creatorcontrib><description>Objectives:
The aim of this study was to evaluate the effects of intense pulsed light (IPL) irradiation on skin fibroblasts
in vitro,
as well as to explore the biomolecular mechanisms and ultrastructure changes of the IPL effect.
Background:
IPL is frequently used to improve telangiectasias, lentigos, and skin texture. Clinical studies have demonstrated that IPL has significant photorejuvenation effects on photoaged skin. However, the biomolecular mechanisms underlying the photorejuvenation of IPL treatment remain largely unknown.
Methods:
Human skin fibroblasts cultured
in vitro
were irradiated with IPL via double pulses of 4 ms and 6 ms with a pulse interval of 20 ms and fluences of 18, 23, 28, and 33 J/cm
2
. Twenty-four hours after irradiation, cell viability, cell cycle distribution, mRNA and protein levels of procollagen (types I and III), and changes of ultrastructure were evaluated using methyl thiazole tetrazolium (MTT) assay, flow cytometry, real-time reverse transcriptase polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assays (ELISA), and transmission electron microscopy, respectively.
Results:
IPL irradiation resulted in the improvement of cell viability of skin fibroblasts in a dose-dependent manner. There were obvious changes in ultrastructure of fibroblasts, compared with the control group. The percentage of fibroblasts at the S and G
2
/M stage of the cell cycle was significantly higher, whereas the percentage at the G
0
/G
1
stage was lower. IPL irradiation increased the mRNA level of collagen I considerably, to 123%, 154%, 172%, and 141% of the control, and that of collagen III to 120%, 141%, 164%, and 132%. IPL irradiation was able to upregulate expression of collagen (types I and III) at the mRNA and protein level.
Conclusions:
The present study demonstrates that IPL irradiation imparts stimulatory effects on skin fibroblasts
in vitro
. This provides valuable evidence of the photorejuvenation effect of IPL
in vivo
.</description><identifier>ISSN: 1549-5418</identifier><identifier>EISSN: 1557-8550</identifier><identifier>DOI: 10.1089/pho.2010.2867</identifier><identifier>PMID: 21438701</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Aging ; Cell Culture Techniques ; Cell Survival - radiation effects ; Collagen - metabolism ; Collagen - radiation effects ; Dermis - physiology ; Dermis - radiation effects ; Dermis - ultrastructure ; Fibroblasts ; Fibroblasts - physiology ; Fibroblasts - radiation effects ; Fibroblasts - ultrastructure ; Health aspects ; Humans ; Irradiation ; Original Research ; Phototherapy ; Physiological aspects ; Skin ; Skin Aging - pathology ; Skin Aging - radiation effects</subject><ispartof>Photomedicine and laser surgery, 2011-05, Vol.29 (5), p.327-332</ispartof><rights>2011, Mary Ann Liebert, Inc.</rights><rights>COPYRIGHT 2011 Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-3a68a297cb9b9710ff2fb05739ec2a38926be526f027fca3130624e2fee005c33</citedby><cites>FETCH-LOGICAL-c403t-3a68a297cb9b9710ff2fb05739ec2a38926be526f027fca3130624e2fee005c33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21438701$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cao, Yongqian</creatorcontrib><creatorcontrib>Huo, Ran</creatorcontrib><creatorcontrib>Feng, Yongqiang</creatorcontrib><creatorcontrib>Li, Qiang</creatorcontrib><creatorcontrib>Wang, Fagang</creatorcontrib><title>Effects of Intense Pulsed Light on the Biological Properties and Ultrastructure of Skin Dermal Fibroblasts: Potential Roles in Photoaging</title><title>Photomedicine and laser surgery</title><addtitle>Photomed Laser Surg</addtitle><description>Objectives:
The aim of this study was to evaluate the effects of intense pulsed light (IPL) irradiation on skin fibroblasts
in vitro,
as well as to explore the biomolecular mechanisms and ultrastructure changes of the IPL effect.
Background:
IPL is frequently used to improve telangiectasias, lentigos, and skin texture. Clinical studies have demonstrated that IPL has significant photorejuvenation effects on photoaged skin. However, the biomolecular mechanisms underlying the photorejuvenation of IPL treatment remain largely unknown.
Methods:
Human skin fibroblasts cultured
in vitro
were irradiated with IPL via double pulses of 4 ms and 6 ms with a pulse interval of 20 ms and fluences of 18, 23, 28, and 33 J/cm
2
. Twenty-four hours after irradiation, cell viability, cell cycle distribution, mRNA and protein levels of procollagen (types I and III), and changes of ultrastructure were evaluated using methyl thiazole tetrazolium (MTT) assay, flow cytometry, real-time reverse transcriptase polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assays (ELISA), and transmission electron microscopy, respectively.
Results:
IPL irradiation resulted in the improvement of cell viability of skin fibroblasts in a dose-dependent manner. There were obvious changes in ultrastructure of fibroblasts, compared with the control group. The percentage of fibroblasts at the S and G
2
/M stage of the cell cycle was significantly higher, whereas the percentage at the G
0
/G
1
stage was lower. IPL irradiation increased the mRNA level of collagen I considerably, to 123%, 154%, 172%, and 141% of the control, and that of collagen III to 120%, 141%, 164%, and 132%. IPL irradiation was able to upregulate expression of collagen (types I and III) at the mRNA and protein level.
Conclusions:
The present study demonstrates that IPL irradiation imparts stimulatory effects on skin fibroblasts
in vitro
. This provides valuable evidence of the photorejuvenation effect of IPL
in vivo
.</description><subject>Aging</subject><subject>Cell Culture Techniques</subject><subject>Cell Survival - radiation effects</subject><subject>Collagen - metabolism</subject><subject>Collagen - radiation effects</subject><subject>Dermis - physiology</subject><subject>Dermis - radiation effects</subject><subject>Dermis - ultrastructure</subject><subject>Fibroblasts</subject><subject>Fibroblasts - physiology</subject><subject>Fibroblasts - radiation effects</subject><subject>Fibroblasts - ultrastructure</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Irradiation</subject><subject>Original Research</subject><subject>Phototherapy</subject><subject>Physiological aspects</subject><subject>Skin</subject><subject>Skin Aging - pathology</subject><subject>Skin Aging - radiation effects</subject><issn>1549-5418</issn><issn>1557-8550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-LFDEQxYMo7rp69CoBD556zJ9Op9vbuu7qwoCDuueQzlR6oulkTNIHP4Lf2jSzCoIgOST1-NWrFA-h55RsKOmH18dD3DBSK9Z38gE6p0LIpheCPFzf7dCIlvZn6EnOXwlhgxz4Y3TGaMt7Seg5-nltLZiScbT4NhQIGfBu8Rn2eOumQ8Ex4HIA_NZFHydntMe7FI-QioOMddjjO1-SziUtpiwJVp_P31zA7yDNFb5xY4qjr0B-g3exDiiuyp-ir-0V2x1iiXpyYXqKHlldBz-7vy_Q3c31l6sPzfbj-9ury21jWsJLw3XX67qHGYdxkJRYy-xIhOQDGKZ5P7BuBME6S5i0RnPKScdaYBaAEGE4v0CvTr7HFL8vkIuaXTbgvQ4Ql6z6riOtpJJW8uWJnLQH5YKNdVOz0uqSCUlZL7rVb_MPqp49zM7EANZV_a-G5tRgUsw5gVXH5GadfihK1JqpqpmqNVO1Zlr5F_cfXsYZ9n_o3yFWgJ-AVdYheAdjDeg_tr8A8ymuKQ</recordid><startdate>20110501</startdate><enddate>20110501</enddate><creator>Cao, Yongqian</creator><creator>Huo, Ran</creator><creator>Feng, Yongqiang</creator><creator>Li, Qiang</creator><creator>Wang, Fagang</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110501</creationdate><title>Effects of Intense Pulsed Light on the Biological Properties and Ultrastructure of Skin Dermal Fibroblasts: Potential Roles in Photoaging</title><author>Cao, Yongqian ; Huo, Ran ; Feng, Yongqiang ; Li, Qiang ; Wang, Fagang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-3a68a297cb9b9710ff2fb05739ec2a38926be526f027fca3130624e2fee005c33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Aging</topic><topic>Cell Culture Techniques</topic><topic>Cell Survival - radiation effects</topic><topic>Collagen - metabolism</topic><topic>Collagen - radiation effects</topic><topic>Dermis - physiology</topic><topic>Dermis - radiation effects</topic><topic>Dermis - ultrastructure</topic><topic>Fibroblasts</topic><topic>Fibroblasts - physiology</topic><topic>Fibroblasts - radiation effects</topic><topic>Fibroblasts - ultrastructure</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Irradiation</topic><topic>Original Research</topic><topic>Phototherapy</topic><topic>Physiological aspects</topic><topic>Skin</topic><topic>Skin Aging - pathology</topic><topic>Skin Aging - radiation effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cao, Yongqian</creatorcontrib><creatorcontrib>Huo, Ran</creatorcontrib><creatorcontrib>Feng, Yongqiang</creatorcontrib><creatorcontrib>Li, Qiang</creatorcontrib><creatorcontrib>Wang, Fagang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Photomedicine and laser surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cao, Yongqian</au><au>Huo, Ran</au><au>Feng, Yongqiang</au><au>Li, Qiang</au><au>Wang, Fagang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Intense Pulsed Light on the Biological Properties and Ultrastructure of Skin Dermal Fibroblasts: Potential Roles in Photoaging</atitle><jtitle>Photomedicine and laser surgery</jtitle><addtitle>Photomed Laser Surg</addtitle><date>2011-05-01</date><risdate>2011</risdate><volume>29</volume><issue>5</issue><spage>327</spage><epage>332</epage><pages>327-332</pages><issn>1549-5418</issn><eissn>1557-8550</eissn><abstract>Objectives:
The aim of this study was to evaluate the effects of intense pulsed light (IPL) irradiation on skin fibroblasts
in vitro,
as well as to explore the biomolecular mechanisms and ultrastructure changes of the IPL effect.
Background:
IPL is frequently used to improve telangiectasias, lentigos, and skin texture. Clinical studies have demonstrated that IPL has significant photorejuvenation effects on photoaged skin. However, the biomolecular mechanisms underlying the photorejuvenation of IPL treatment remain largely unknown.
Methods:
Human skin fibroblasts cultured
in vitro
were irradiated with IPL via double pulses of 4 ms and 6 ms with a pulse interval of 20 ms and fluences of 18, 23, 28, and 33 J/cm
2
. Twenty-four hours after irradiation, cell viability, cell cycle distribution, mRNA and protein levels of procollagen (types I and III), and changes of ultrastructure were evaluated using methyl thiazole tetrazolium (MTT) assay, flow cytometry, real-time reverse transcriptase polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assays (ELISA), and transmission electron microscopy, respectively.
Results:
IPL irradiation resulted in the improvement of cell viability of skin fibroblasts in a dose-dependent manner. There were obvious changes in ultrastructure of fibroblasts, compared with the control group. The percentage of fibroblasts at the S and G
2
/M stage of the cell cycle was significantly higher, whereas the percentage at the G
0
/G
1
stage was lower. IPL irradiation increased the mRNA level of collagen I considerably, to 123%, 154%, 172%, and 141% of the control, and that of collagen III to 120%, 141%, 164%, and 132%. IPL irradiation was able to upregulate expression of collagen (types I and III) at the mRNA and protein level.
Conclusions:
The present study demonstrates that IPL irradiation imparts stimulatory effects on skin fibroblasts
in vitro
. This provides valuable evidence of the photorejuvenation effect of IPL
in vivo
.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>21438701</pmid><doi>10.1089/pho.2010.2867</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1549-5418 |
| ispartof | Photomedicine and laser surgery, 2011-05, Vol.29 (5), p.327-332 |
| issn | 1549-5418 1557-8550 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_866047171 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Aging Cell Culture Techniques Cell Survival - radiation effects Collagen - metabolism Collagen - radiation effects Dermis - physiology Dermis - radiation effects Dermis - ultrastructure Fibroblasts Fibroblasts - physiology Fibroblasts - radiation effects Fibroblasts - ultrastructure Health aspects Humans Irradiation Original Research Phototherapy Physiological aspects Skin Skin Aging - pathology Skin Aging - radiation effects |
| title | Effects of Intense Pulsed Light on the Biological Properties and Ultrastructure of Skin Dermal Fibroblasts: Potential Roles in Photoaging |
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