Voltammetric determination of epinephrine by White rot fungi ( Phanerochaete chrysosporium ME446) cells based microbial biosensor
The lyophilized biomass of White rot fungi ( Phanerochaete chrysosporium ME446) was immobilized in gelatine using glutaraldehyde crosslinking agent on a Pt working electrode. The fungal cells retained their laccase activity under entrapped state. The immobilized cells were used as a source of laccas...
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| Veröffentlicht in: | Biosensors & bioelectronics 2011-01, Vol.26 (5), p.2590-2594 |
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| creator | Akyilmaz, Erol Turemis, Mehmet Yasa, İhsan |
| description | The lyophilized biomass of White rot fungi (
Phanerochaete chrysosporium ME446) was immobilized in gelatine using glutaraldehyde crosslinking agent on a Pt working electrode. The fungal cells retained their laccase activity under entrapped state. The immobilized cells were used as a source of laccase to develop amperometric epinephrine biosensor. The catalytic action of the laccase in the biosensor released an epinephrinequinone as a result of redox activity, thereby causing an increase in the current. The optimal working conditions of the biosensor were carried out at pH 4.5 (50
mM acetate buffer containing 100
mM K
3Fe(CN)
6), and 20
°C. The sensor response was linear over a range of 5–100
μM epinephrine. The detection limit of the biosensor was found to be 1.04
μM. In the optimization and characterization studies of the microbial biosensor some parameters such as effect of fungi and gelatine amount, percentage of glutaraldehyde on the biosensor response and substrate specificity were carried out. In the application studies of the biosensor, sensitive determination of epinephrine in pharmaceutical ampules was investigated. |
| doi_str_mv | 10.1016/j.bios.2010.11.012 |
| format | Article |
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Phanerochaete chrysosporium ME446) was immobilized in gelatine using glutaraldehyde crosslinking agent on a Pt working electrode. The fungal cells retained their laccase activity under entrapped state. The immobilized cells were used as a source of laccase to develop amperometric epinephrine biosensor. The catalytic action of the laccase in the biosensor released an epinephrinequinone as a result of redox activity, thereby causing an increase in the current. The optimal working conditions of the biosensor were carried out at pH 4.5 (50
mM acetate buffer containing 100
mM K
3Fe(CN)
6), and 20
°C. The sensor response was linear over a range of 5–100
μM epinephrine. The detection limit of the biosensor was found to be 1.04
μM. In the optimization and characterization studies of the microbial biosensor some parameters such as effect of fungi and gelatine amount, percentage of glutaraldehyde on the biosensor response and substrate specificity were carried out. In the application studies of the biosensor, sensitive determination of epinephrine in pharmaceutical ampules was investigated.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2010.11.012</identifier><identifier>PMID: 21115238</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>acetates ; Acetic acid ; Ampules ; Biological and medical sciences ; Biological Assay - instrumentation ; Biomass ; Biosensing Techniques - instrumentation ; Biosensor ; Biosensors ; Biotechnology ; Conductometry - instrumentation ; crosslinking ; detection limit ; Electrodes ; electronic sensing ; enzyme activity ; Epinephrine ; Epinephrine - analysis ; Epinephrine - pharmacology ; Equipment Design ; Equipment Failure Analysis ; freeze drying ; Fundamental and applied biological sciences. Psychology ; Fungi ; gelatin ; Glutaraldehyde ; Immobilized cells ; Laccase ; Methods. Procedures. Technologies ; Microbial ; pH effects ; Phanerochaete - drug effects ; Phanerochaete chrysosporium ; Pharmaceuticals ; Redox properties ; redox reactions ; Substrate specificity ; Various methods and equipments ; Voltammetry ; White rot ; white-rot fungi ; working conditions</subject><ispartof>Biosensors & bioelectronics, 2011-01, Vol.26 (5), p.2590-2594</ispartof><rights>2010 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-f843ee775d6e2c1e0666029914feaa81e9b79f941f8ba570d2645beaea6eae143</citedby><cites>FETCH-LOGICAL-c441t-f843ee775d6e2c1e0666029914feaa81e9b79f941f8ba570d2645beaea6eae143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2010.11.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27926,27927,45997</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23844114$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21115238$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Akyilmaz, Erol</creatorcontrib><creatorcontrib>Turemis, Mehmet</creatorcontrib><creatorcontrib>Yasa, İhsan</creatorcontrib><title>Voltammetric determination of epinephrine by White rot fungi ( Phanerochaete chrysosporium ME446) cells based microbial biosensor</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>The lyophilized biomass of White rot fungi (
Phanerochaete chrysosporium ME446) was immobilized in gelatine using glutaraldehyde crosslinking agent on a Pt working electrode. The fungal cells retained their laccase activity under entrapped state. The immobilized cells were used as a source of laccase to develop amperometric epinephrine biosensor. The catalytic action of the laccase in the biosensor released an epinephrinequinone as a result of redox activity, thereby causing an increase in the current. The optimal working conditions of the biosensor were carried out at pH 4.5 (50
mM acetate buffer containing 100
mM K
3Fe(CN)
6), and 20
°C. The sensor response was linear over a range of 5–100
μM epinephrine. The detection limit of the biosensor was found to be 1.04
μM. In the optimization and characterization studies of the microbial biosensor some parameters such as effect of fungi and gelatine amount, percentage of glutaraldehyde on the biosensor response and substrate specificity were carried out. In the application studies of the biosensor, sensitive determination of epinephrine in pharmaceutical ampules was investigated.</description><subject>acetates</subject><subject>Acetic acid</subject><subject>Ampules</subject><subject>Biological and medical sciences</subject><subject>Biological Assay - instrumentation</subject><subject>Biomass</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biosensor</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Conductometry - instrumentation</subject><subject>crosslinking</subject><subject>detection limit</subject><subject>Electrodes</subject><subject>electronic sensing</subject><subject>enzyme activity</subject><subject>Epinephrine</subject><subject>Epinephrine - analysis</subject><subject>Epinephrine - pharmacology</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>freeze drying</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungi</subject><subject>gelatin</subject><subject>Glutaraldehyde</subject><subject>Immobilized cells</subject><subject>Laccase</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbial</subject><subject>pH effects</subject><subject>Phanerochaete - drug effects</subject><subject>Phanerochaete chrysosporium</subject><subject>Pharmaceuticals</subject><subject>Redox properties</subject><subject>redox reactions</subject><subject>Substrate specificity</subject><subject>Various methods and equipments</subject><subject>Voltammetry</subject><subject>White rot</subject><subject>white-rot fungi</subject><subject>working conditions</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EosvCF-AAviDgkMXjOE4icUFV-SMVgQSFo-U448arJE7tBGmPfHOc7gI3uMxI1m_G780j5DGwHTCQr_a7xvm442x9gB0DfodsoCrzTPC8uEs2rC5kVkiZn5EHMe4ZYyXU7D454wBQ8LzakJ_ffD_rYcA5OENbnDEMbtSz8yP1luLkRpy6kCptDvR752akwc_ULuO1oy_o506PGLzpdBqlpguH6OPkg1sG-vFCCPmSGuz7SBsdsaWDM8E3Tvd0lY5j9OEhuWd1H_HRqW_J1duLr-fvs8tP7z6cv7nMjBAwZ7YSOWJZFq1EbgCZlJLxugZhUesKsG7K2tYCbNXoomQtl6JoUKOWqYDIt-T5ce8U_M2CcVaDi6u2ZMAvUVVS1BKKov4_yXkueZmOvCX8SCZXMQa0agpu0OGggKk1I7VXq1G1ZqQAVMooDT05rV-aAds_I79DScCzE6Cj0b0NejQu_uXyKl3k1tHTI2e1V_o6JObqS_qpuA26BJmI10cC02F_OAwqGoejwdYFNLNqvfuX0l9IMbo3</recordid><startdate>20110115</startdate><enddate>20110115</enddate><creator>Akyilmaz, Erol</creator><creator>Turemis, Mehmet</creator><creator>Yasa, İhsan</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20110115</creationdate><title>Voltammetric determination of epinephrine by White rot fungi ( Phanerochaete chrysosporium ME446) cells based microbial biosensor</title><author>Akyilmaz, Erol ; Turemis, Mehmet ; Yasa, İhsan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-f843ee775d6e2c1e0666029914feaa81e9b79f941f8ba570d2645beaea6eae143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>acetates</topic><topic>Acetic acid</topic><topic>Ampules</topic><topic>Biological and medical sciences</topic><topic>Biological Assay - instrumentation</topic><topic>Biomass</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensor</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Conductometry - instrumentation</topic><topic>crosslinking</topic><topic>detection limit</topic><topic>Electrodes</topic><topic>electronic sensing</topic><topic>enzyme activity</topic><topic>Epinephrine</topic><topic>Epinephrine - analysis</topic><topic>Epinephrine - pharmacology</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>freeze drying</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungi</topic><topic>gelatin</topic><topic>Glutaraldehyde</topic><topic>Immobilized cells</topic><topic>Laccase</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbial</topic><topic>pH effects</topic><topic>Phanerochaete - drug effects</topic><topic>Phanerochaete chrysosporium</topic><topic>Pharmaceuticals</topic><topic>Redox properties</topic><topic>redox reactions</topic><topic>Substrate specificity</topic><topic>Various methods and equipments</topic><topic>Voltammetry</topic><topic>White rot</topic><topic>white-rot fungi</topic><topic>working conditions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Akyilmaz, Erol</creatorcontrib><creatorcontrib>Turemis, Mehmet</creatorcontrib><creatorcontrib>Yasa, İhsan</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Akyilmaz, Erol</au><au>Turemis, Mehmet</au><au>Yasa, İhsan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Voltammetric determination of epinephrine by White rot fungi ( Phanerochaete chrysosporium ME446) cells based microbial biosensor</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2011-01-15</date><risdate>2011</risdate><volume>26</volume><issue>5</issue><spage>2590</spage><epage>2594</epage><pages>2590-2594</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>The lyophilized biomass of White rot fungi (
Phanerochaete chrysosporium ME446) was immobilized in gelatine using glutaraldehyde crosslinking agent on a Pt working electrode. The fungal cells retained their laccase activity under entrapped state. The immobilized cells were used as a source of laccase to develop amperometric epinephrine biosensor. The catalytic action of the laccase in the biosensor released an epinephrinequinone as a result of redox activity, thereby causing an increase in the current. The optimal working conditions of the biosensor were carried out at pH 4.5 (50
mM acetate buffer containing 100
mM K
3Fe(CN)
6), and 20
°C. The sensor response was linear over a range of 5–100
μM epinephrine. The detection limit of the biosensor was found to be 1.04
μM. In the optimization and characterization studies of the microbial biosensor some parameters such as effect of fungi and gelatine amount, percentage of glutaraldehyde on the biosensor response and substrate specificity were carried out. In the application studies of the biosensor, sensitive determination of epinephrine in pharmaceutical ampules was investigated.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>21115238</pmid><doi>10.1016/j.bios.2010.11.012</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0956-5663 |
| ispartof | Biosensors & bioelectronics, 2011-01, Vol.26 (5), p.2590-2594 |
| issn | 0956-5663 1873-4235 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_864961559 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | acetates Acetic acid Ampules Biological and medical sciences Biological Assay - instrumentation Biomass Biosensing Techniques - instrumentation Biosensor Biosensors Biotechnology Conductometry - instrumentation crosslinking detection limit Electrodes electronic sensing enzyme activity Epinephrine Epinephrine - analysis Epinephrine - pharmacology Equipment Design Equipment Failure Analysis freeze drying Fundamental and applied biological sciences. Psychology Fungi gelatin Glutaraldehyde Immobilized cells Laccase Methods. Procedures. Technologies Microbial pH effects Phanerochaete - drug effects Phanerochaete chrysosporium Pharmaceuticals Redox properties redox reactions Substrate specificity Various methods and equipments Voltammetry White rot white-rot fungi working conditions |
| title | Voltammetric determination of epinephrine by White rot fungi ( Phanerochaete chrysosporium ME446) cells based microbial biosensor |
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