Optical imaging of intracellular reactive oxygen species for the assessment of the cytotoxicity of nanoparticles
Abstract The generation of intracellular reactive oxygen species (ROS) was optically monitored using ROS-sensitive gold nanoprobes in response to an exposure of nanoparticles (NPs). Fluorescent dye-labeled hyaluronic acid was grafted onto the surface of gold nanoparticles (HF-AuNPs) for imaging intr...
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| Veröffentlicht in: | Biomaterials 2011-04, Vol.32 (10), p.2556-2565 |
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| creator | Lee, Kyuri Lee, Hyukjin Lee, Kun Woo Park, Tae Gwan |
| description | Abstract The generation of intracellular reactive oxygen species (ROS) was optically monitored using ROS-sensitive gold nanoprobes in response to an exposure of nanoparticles (NPs). Fluorescent dye-labeled hyaluronic acid was grafted onto the surface of gold nanoparticles (HF-AuNPs) for imaging intracellular ROS. The ultrasensitive detection of intracellular ROS was utilized as a powerful analytical tool to assess early cellular toxicities of monodisperse polystyrene (PS) particles with different sizes and different functional groups on the surface. The effect of PEGylation on the surface of PS NPs was also investigated by evaluating intracellular ROS generation. For various PS NPs, the extent of intracellular ROS was well correlated with cellular uptake, apoptosis inducing activity, and cytotoxic effect of NPs. In addition to the nanoparticles, commonly used polymeric gene carriers such as linear and branched polyethylenimine (PEI) were tested to analyze their extent of intracellular ROS generation related to cellular toxicity. This study demonstrated that sensitive and optical detection of intracellular ROS generation can provide a valuable toxicity index value for a wide range of NPs as an early indicator for cellular responses. |
| doi_str_mv | 10.1016/j.biomaterials.2010.11.072 |
| format | Article |
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Fluorescent dye-labeled hyaluronic acid was grafted onto the surface of gold nanoparticles (HF-AuNPs) for imaging intracellular ROS. The ultrasensitive detection of intracellular ROS was utilized as a powerful analytical tool to assess early cellular toxicities of monodisperse polystyrene (PS) particles with different sizes and different functional groups on the surface. The effect of PEGylation on the surface of PS NPs was also investigated by evaluating intracellular ROS generation. For various PS NPs, the extent of intracellular ROS was well correlated with cellular uptake, apoptosis inducing activity, and cytotoxic effect of NPs. In addition to the nanoparticles, commonly used polymeric gene carriers such as linear and branched polyethylenimine (PEI) were tested to analyze their extent of intracellular ROS generation related to cellular toxicity. This study demonstrated that sensitive and optical detection of intracellular ROS generation can provide a valuable toxicity index value for a wide range of NPs as an early indicator for cellular responses.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2010.11.072</identifier><identifier>PMID: 21247630</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Advanced Basic Science ; Animals ; Apoptosis ; Cell Death - drug effects ; Cell Line ; Computed tomography ; Cytotoxicity ; Dentistry ; Gold ; Gold - toxicity ; Gold nanoprobes ; Hyaluronic acid ; Hyaluronic Acid - chemistry ; Hyaluronic Acid - toxicity ; Imaging, Three-Dimensional - methods ; Intracellular Space - metabolism ; Metal Nanoparticles - chemistry ; Metal Nanoparticles - toxicity ; Mice ; nanoparticles ; Nanotoxicity ; Optical Phenomena ; Particle Size ; PEGylation ; poly(ethylenimine) ; Polyethylene Glycols - pharmacology ; Polyethyleneimine - chemistry ; Polyethyleneimine - toxicity ; polystyrene ; Polystyrenes - toxicity ; Reactive oxygen species ; Reactive oxygen species (ROS) ; Reactive Oxygen Species - metabolism ; Rhodamines - metabolism ; Surface Properties - drug effects ; Toxicity ; Viruses - drug effects</subject><ispartof>Biomaterials, 2011-04, Vol.32 (10), p.2556-2565</ispartof><rights>Elsevier Ltd</rights><rights>2010 Elsevier Ltd</rights><rights>Copyright © 2010 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-8b2db95273d1828755714f4c8d6af3357d9fb6a5c617c3a49cdc69694a9dccff3</citedby><cites>FETCH-LOGICAL-c532t-8b2db95273d1828755714f4c8d6af3357d9fb6a5c617c3a49cdc69694a9dccff3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0142961210015279$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21247630$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Kyuri</creatorcontrib><creatorcontrib>Lee, Hyukjin</creatorcontrib><creatorcontrib>Lee, Kun Woo</creatorcontrib><creatorcontrib>Park, Tae Gwan</creatorcontrib><title>Optical imaging of intracellular reactive oxygen species for the assessment of the cytotoxicity of nanoparticles</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract The generation of intracellular reactive oxygen species (ROS) was optically monitored using ROS-sensitive gold nanoprobes in response to an exposure of nanoparticles (NPs). Fluorescent dye-labeled hyaluronic acid was grafted onto the surface of gold nanoparticles (HF-AuNPs) for imaging intracellular ROS. The ultrasensitive detection of intracellular ROS was utilized as a powerful analytical tool to assess early cellular toxicities of monodisperse polystyrene (PS) particles with different sizes and different functional groups on the surface. The effect of PEGylation on the surface of PS NPs was also investigated by evaluating intracellular ROS generation. For various PS NPs, the extent of intracellular ROS was well correlated with cellular uptake, apoptosis inducing activity, and cytotoxic effect of NPs. In addition to the nanoparticles, commonly used polymeric gene carriers such as linear and branched polyethylenimine (PEI) were tested to analyze their extent of intracellular ROS generation related to cellular toxicity. This study demonstrated that sensitive and optical detection of intracellular ROS generation can provide a valuable toxicity index value for a wide range of NPs as an early indicator for cellular responses.</description><subject>Advanced Basic Science</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Cell Death - drug effects</subject><subject>Cell Line</subject><subject>Computed tomography</subject><subject>Cytotoxicity</subject><subject>Dentistry</subject><subject>Gold</subject><subject>Gold - toxicity</subject><subject>Gold nanoprobes</subject><subject>Hyaluronic acid</subject><subject>Hyaluronic Acid - chemistry</subject><subject>Hyaluronic Acid - toxicity</subject><subject>Imaging, Three-Dimensional - methods</subject><subject>Intracellular Space - metabolism</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Metal Nanoparticles - toxicity</subject><subject>Mice</subject><subject>nanoparticles</subject><subject>Nanotoxicity</subject><subject>Optical Phenomena</subject><subject>Particle Size</subject><subject>PEGylation</subject><subject>poly(ethylenimine)</subject><subject>Polyethylene Glycols - pharmacology</subject><subject>Polyethyleneimine - chemistry</subject><subject>Polyethyleneimine - toxicity</subject><subject>polystyrene</subject><subject>Polystyrenes - toxicity</subject><subject>Reactive oxygen species</subject><subject>Reactive oxygen species (ROS)</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Rhodamines - metabolism</subject><subject>Surface Properties - drug effects</subject><subject>Toxicity</subject><subject>Viruses - drug effects</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUGP1SAUhYnROG9G_4Jp3LjqE2ihxYWJGR01mWQWjmtC6eXJs4UKdDL99wN5ozGuXBEu59zL_Q5CrwneE0z42-N-sH5WCYJVU9xTXB7IHnf0CdqRvutrJjB7inaYtLQWnNAzdB7jEec7bulzdEYJbTve4B1abpZktZoqO6uDdYfKm8q6FJSGaVonFaoASid7B5W_3w7gqriAthAr40OVfkClYoQYZ3CpeEtFb8knf2-1TVupOeX8okKeM0F8gZ6Z_Gt4-XheoO9Xn24vv9TXN5-_Xn64rjVraKr7gY6DYLRrRtLTvmOsI61pdT9yZZqGdaMwA1dMc9LpRrVCj5oLLlolRq2NaS7Qm1PfJfhfK8QkZxvLUsqBX6PseSs4Zi3NyncnpQ4-xgBGLiHTCJskWBbg8ij_Bi4LcEmIzMCz-dXjmHWYYfxj_U04Cz6eBJCXvbMQZMz4nIbRBtBJjt7-35z3_7TRk3UluZ-wQTz6NbjiITJSieW3En1JnuTQM0XRPACGr7B2</recordid><startdate>20110401</startdate><enddate>20110401</enddate><creator>Lee, Kyuri</creator><creator>Lee, Hyukjin</creator><creator>Lee, Kun Woo</creator><creator>Park, Tae Gwan</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20110401</creationdate><title>Optical imaging of intracellular reactive oxygen species for the assessment of the cytotoxicity of nanoparticles</title><author>Lee, Kyuri ; Lee, Hyukjin ; Lee, Kun Woo ; Park, Tae Gwan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c532t-8b2db95273d1828755714f4c8d6af3357d9fb6a5c617c3a49cdc69694a9dccff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Advanced Basic Science</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Cell Death - drug effects</topic><topic>Cell Line</topic><topic>Computed tomography</topic><topic>Cytotoxicity</topic><topic>Dentistry</topic><topic>Gold</topic><topic>Gold - toxicity</topic><topic>Gold nanoprobes</topic><topic>Hyaluronic acid</topic><topic>Hyaluronic Acid - chemistry</topic><topic>Hyaluronic Acid - toxicity</topic><topic>Imaging, Three-Dimensional - methods</topic><topic>Intracellular Space - metabolism</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Metal Nanoparticles - toxicity</topic><topic>Mice</topic><topic>nanoparticles</topic><topic>Nanotoxicity</topic><topic>Optical Phenomena</topic><topic>Particle Size</topic><topic>PEGylation</topic><topic>poly(ethylenimine)</topic><topic>Polyethylene Glycols - pharmacology</topic><topic>Polyethyleneimine - chemistry</topic><topic>Polyethyleneimine - toxicity</topic><topic>polystyrene</topic><topic>Polystyrenes - toxicity</topic><topic>Reactive oxygen species</topic><topic>Reactive oxygen species (ROS)</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Rhodamines - metabolism</topic><topic>Surface Properties - drug effects</topic><topic>Toxicity</topic><topic>Viruses - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Kyuri</creatorcontrib><creatorcontrib>Lee, Hyukjin</creatorcontrib><creatorcontrib>Lee, Kun Woo</creatorcontrib><creatorcontrib>Park, Tae Gwan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Kyuri</au><au>Lee, Hyukjin</au><au>Lee, Kun Woo</au><au>Park, Tae Gwan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optical imaging of intracellular reactive oxygen species for the assessment of the cytotoxicity of nanoparticles</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2011-04-01</date><risdate>2011</risdate><volume>32</volume><issue>10</issue><spage>2556</spage><epage>2565</epage><pages>2556-2565</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract The generation of intracellular reactive oxygen species (ROS) was optically monitored using ROS-sensitive gold nanoprobes in response to an exposure of nanoparticles (NPs). Fluorescent dye-labeled hyaluronic acid was grafted onto the surface of gold nanoparticles (HF-AuNPs) for imaging intracellular ROS. The ultrasensitive detection of intracellular ROS was utilized as a powerful analytical tool to assess early cellular toxicities of monodisperse polystyrene (PS) particles with different sizes and different functional groups on the surface. The effect of PEGylation on the surface of PS NPs was also investigated by evaluating intracellular ROS generation. For various PS NPs, the extent of intracellular ROS was well correlated with cellular uptake, apoptosis inducing activity, and cytotoxic effect of NPs. In addition to the nanoparticles, commonly used polymeric gene carriers such as linear and branched polyethylenimine (PEI) were tested to analyze their extent of intracellular ROS generation related to cellular toxicity. This study demonstrated that sensitive and optical detection of intracellular ROS generation can provide a valuable toxicity index value for a wide range of NPs as an early indicator for cellular responses.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>21247630</pmid><doi>10.1016/j.biomaterials.2010.11.072</doi><tpages>10</tpages></addata></record> |
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| subjects | Advanced Basic Science Animals Apoptosis Cell Death - drug effects Cell Line Computed tomography Cytotoxicity Dentistry Gold Gold - toxicity Gold nanoprobes Hyaluronic acid Hyaluronic Acid - chemistry Hyaluronic Acid - toxicity Imaging, Three-Dimensional - methods Intracellular Space - metabolism Metal Nanoparticles - chemistry Metal Nanoparticles - toxicity Mice nanoparticles Nanotoxicity Optical Phenomena Particle Size PEGylation poly(ethylenimine) Polyethylene Glycols - pharmacology Polyethyleneimine - chemistry Polyethyleneimine - toxicity polystyrene Polystyrenes - toxicity Reactive oxygen species Reactive oxygen species (ROS) Reactive Oxygen Species - metabolism Rhodamines - metabolism Surface Properties - drug effects Toxicity Viruses - drug effects |
| title | Optical imaging of intracellular reactive oxygen species for the assessment of the cytotoxicity of nanoparticles |
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