Protection of pigs against Chlamydia trachomatis challenge by administration of a MOMP-based DNA vaccine in the vaginal mucosa
Abstract Plasmid DNA (pWRG7079::MOMP) expressing the major outer membrane protein of a human Chlamydia trachomatis serovar E strain was tested for the ability to induce an immune response and protect against experimental genital infection with the same serovar. The vaccine was tested in pigs, as the...
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| Veröffentlicht in: | Vaccine 2011-02, Vol.29 (7), p.1399-1407 |
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| creator | Schautteet, K Stuyven, E Beeckman, D.S.A Van Acker, S Carlon, M Chiers, K Cox, E Vanrompay, D |
| description | Abstract Plasmid DNA (pWRG7079::MOMP) expressing the major outer membrane protein of a human Chlamydia trachomatis serovar E strain was tested for the ability to induce an immune response and protect against experimental genital infection with the same serovar. The vaccine was tested in pigs, as they are genetically and physiologically related to humans and suitable for studying C. trachomatis infection of the genital system. To increase the immune response, GM-CSF, LTA and B and CpG motives were used as adjuvants. GM-CSF was administered seven days before immunization, while the other adjuvants were administered together with the vaccine. Ten pigs were randomly divided into two groups. One group received an intravaginal primo-vaccination and a booster of 500 μg pWRG7079::MOMP, while the other group received the placebo vaccine pWRG7079. All animals were challenged with 108 TCID50 of C. trachomatis serovar E. Pigs immunized with the DNA vaccine showed significantly less macroscopic lesions, vaginal excretion and chlamydial replication in the genital tract, as compared to placebo-vaccinated controls. However, infection could not be completely cleared. |
| doi_str_mv | 10.1016/j.vaccine.2010.12.042 |
| format | Article |
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The vaccine was tested in pigs, as they are genetically and physiologically related to humans and suitable for studying C. trachomatis infection of the genital system. To increase the immune response, GM-CSF, LTA and B and CpG motives were used as adjuvants. GM-CSF was administered seven days before immunization, while the other adjuvants were administered together with the vaccine. Ten pigs were randomly divided into two groups. One group received an intravaginal primo-vaccination and a booster of 500 μg pWRG7079::MOMP, while the other group received the placebo vaccine pWRG7079. All animals were challenged with 108 TCID50 of C. trachomatis serovar E. Pigs immunized with the DNA vaccine showed significantly less macroscopic lesions, vaginal excretion and chlamydial replication in the genital tract, as compared to placebo-vaccinated controls. However, infection could not be completely cleared.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2010.12.042</identifier><identifier>PMID: 21195805</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject><![CDATA[adjuvants ; Adjuvants, Immunologic - administration & dosage ; Administration, Intravaginal ; Allergy and Immunology ; Animals ; Antibodies, Bacterial - blood ; Antibody Specificity ; Applied microbiology ; Bacterial Outer Membrane Proteins - administration & dosage ; Bacterial Outer Membrane Proteins - immunology ; Bacterial Shedding ; Bacterial Vaccines - administration & dosage ; Bacterial Vaccines - immunology ; Bacteriology ; Biological and medical sciences ; Cell Proliferation ; Chlamydia Infections - immunology ; Chlamydia Infections - prevention & control ; Chlamydia trachomatis ; Chlamydia trachomatis - immunology ; Cytokines - immunology ; Deoxyribonucleic acid ; DNA ; excretion ; Female ; Fundamental and applied biological sciences. Psychology ; Genital infection ; granulocyte-macrophage colony-stimulating factor ; humans ; Immune response ; Immune system ; Immunization ; Infections ; Leukocytes, Mononuclear - immunology ; Lymphocytes ; Microbiology ; Miscellaneous ; outer membrane proteins ; Pigs ; plasmids ; Porins - administration & dosage ; Porins - immunology ; recombinant vaccines ; serotypes ; Sexually transmitted diseases ; STD ; Swine ; Vaccine ; Vaccines ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Vaccines, DNA - administration & dosage ; Vaccines, DNA - immunology ; Vagina - immunology ; Vagina - microbiology ; vaginal mucosa]]></subject><ispartof>Vaccine, 2011-02, Vol.29 (7), p.1399-1407</ispartof><rights>Elsevier Ltd</rights><rights>2010 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Limited Feb 4, 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c533t-9e69fc12bfe3093b64451443d905ede8d03e35e4abc0bb0ae7c427156d7fa9af3</citedby><cites>FETCH-LOGICAL-c533t-9e69fc12bfe3093b64451443d905ede8d03e35e4abc0bb0ae7c427156d7fa9af3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1498110563?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23905579$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21195805$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schautteet, K</creatorcontrib><creatorcontrib>Stuyven, E</creatorcontrib><creatorcontrib>Beeckman, D.S.A</creatorcontrib><creatorcontrib>Van Acker, S</creatorcontrib><creatorcontrib>Carlon, M</creatorcontrib><creatorcontrib>Chiers, K</creatorcontrib><creatorcontrib>Cox, E</creatorcontrib><creatorcontrib>Vanrompay, D</creatorcontrib><title>Protection of pigs against Chlamydia trachomatis challenge by administration of a MOMP-based DNA vaccine in the vaginal mucosa</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Abstract Plasmid DNA (pWRG7079::MOMP) expressing the major outer membrane protein of a human Chlamydia trachomatis serovar E strain was tested for the ability to induce an immune response and protect against experimental genital infection with the same serovar. The vaccine was tested in pigs, as they are genetically and physiologically related to humans and suitable for studying C. trachomatis infection of the genital system. To increase the immune response, GM-CSF, LTA and B and CpG motives were used as adjuvants. GM-CSF was administered seven days before immunization, while the other adjuvants were administered together with the vaccine. Ten pigs were randomly divided into two groups. One group received an intravaginal primo-vaccination and a booster of 500 μg pWRG7079::MOMP, while the other group received the placebo vaccine pWRG7079. All animals were challenged with 108 TCID50 of C. trachomatis serovar E. Pigs immunized with the DNA vaccine showed significantly less macroscopic lesions, vaginal excretion and chlamydial replication in the genital tract, as compared to placebo-vaccinated controls. However, infection could not be completely cleared.</description><subject>adjuvants</subject><subject>Adjuvants, Immunologic - administration & dosage</subject><subject>Administration, Intravaginal</subject><subject>Allergy and Immunology</subject><subject>Animals</subject><subject>Antibodies, Bacterial - blood</subject><subject>Antibody Specificity</subject><subject>Applied microbiology</subject><subject>Bacterial Outer Membrane Proteins - administration & dosage</subject><subject>Bacterial Outer Membrane Proteins - immunology</subject><subject>Bacterial Shedding</subject><subject>Bacterial Vaccines - administration & dosage</subject><subject>Bacterial Vaccines - immunology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cell Proliferation</subject><subject>Chlamydia Infections - immunology</subject><subject>Chlamydia Infections - prevention & control</subject><subject>Chlamydia trachomatis</subject><subject>Chlamydia trachomatis - immunology</subject><subject>Cytokines - immunology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>excretion</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genital infection</subject><subject>granulocyte-macrophage colony-stimulating factor</subject><subject>humans</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunization</subject><subject>Infections</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Lymphocytes</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>outer membrane proteins</subject><subject>Pigs</subject><subject>plasmids</subject><subject>Porins - administration & dosage</subject><subject>Porins - immunology</subject><subject>recombinant vaccines</subject><subject>serotypes</subject><subject>Sexually transmitted diseases</subject><subject>STD</subject><subject>Swine</subject><subject>Vaccine</subject><subject>Vaccines</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Vaccines, DNA - administration & dosage</subject><subject>Vaccines, DNA - immunology</subject><subject>Vagina - immunology</subject><subject>Vagina - microbiology</subject><subject>vaginal mucosa</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkk1v1DAQhiMEokvhJwCWEOKUxY7tfFxA1ZYvqaWVSiVu1sSZ7HpJ4sXOVtpLfzsT7ZZKvfRk2X7m9XjeN0leCz4XXOQf1_MbsNYNOM_4dJbNucqeJDNRFjLNtCifJjOe5SpVgv8-Sl7EuOacaymq58lRJkSlS65nye1l8CPa0fmB-ZZt3DIyWIIb4sgWqw76XeOAjQHsyvcwusjsCroOhyWyeseg6d3gIt3fKQA7vzi_TGuI2LDTnyfs0CZzAxtXSNulG6Bj_db6CC-TZy10EV8d1uPk-uuXX4vv6dnFtx-Lk7PUainHtMK8aq3I6hYlr2SdK6WFUrKpuMYGy4ZLlBoV1JbXNQcsrMoKofOmaKGCVh4nH_a6m-D_bjGOpnfRYtfBgH4bTZkrGojOxeOkKvNSFbki8t0Dcu23gf4WjVBVKQTXuSRK7ykbfIwBW7MJroewM4KbyUmzNocRmclJIzJDTlLdm4P6tu6x-V91Zx0B7w8ARAtdG2CwLt5zkmaji4q4t3uuBW9gGYi5vqKXNOdClkXFifi8J5AcuHEYTLQOB4uNCxQN03j3aLOfHijYjnJBbf3BHcb7uZhIBeZqCuaUS0E9lIIs_ge3_Ny1</recordid><startdate>20110204</startdate><enddate>20110204</enddate><creator>Schautteet, K</creator><creator>Stuyven, E</creator><creator>Beeckman, D.S.A</creator><creator>Van Acker, S</creator><creator>Carlon, M</creator><creator>Chiers, K</creator><creator>Cox, E</creator><creator>Vanrompay, D</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>20110204</creationdate><title>Protection of pigs against Chlamydia trachomatis challenge by administration of a MOMP-based DNA vaccine in the vaginal mucosa</title><author>Schautteet, K ; Stuyven, E ; Beeckman, D.S.A ; Van Acker, S ; Carlon, M ; Chiers, K ; Cox, E ; Vanrompay, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c533t-9e69fc12bfe3093b64451443d905ede8d03e35e4abc0bb0ae7c427156d7fa9af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>adjuvants</topic><topic>Adjuvants, Immunologic - administration & dosage</topic><topic>Administration, Intravaginal</topic><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>Antibodies, Bacterial - blood</topic><topic>Antibody Specificity</topic><topic>Applied microbiology</topic><topic>Bacterial Outer Membrane Proteins - administration & dosage</topic><topic>Bacterial Outer Membrane Proteins - immunology</topic><topic>Bacterial Shedding</topic><topic>Bacterial Vaccines - administration & dosage</topic><topic>Bacterial Vaccines - immunology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cell Proliferation</topic><topic>Chlamydia Infections - immunology</topic><topic>Chlamydia Infections - prevention & control</topic><topic>Chlamydia trachomatis</topic><topic>Chlamydia trachomatis - immunology</topic><topic>Cytokines - immunology</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>excretion</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genital infection</topic><topic>granulocyte-macrophage colony-stimulating factor</topic><topic>humans</topic><topic>Immune response</topic><topic>Immune system</topic><topic>Immunization</topic><topic>Infections</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Lymphocytes</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>outer membrane proteins</topic><topic>Pigs</topic><topic>plasmids</topic><topic>Porins - administration & dosage</topic><topic>Porins - immunology</topic><topic>recombinant vaccines</topic><topic>serotypes</topic><topic>Sexually transmitted diseases</topic><topic>STD</topic><topic>Swine</topic><topic>Vaccine</topic><topic>Vaccines</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Vaccines, DNA - administration & dosage</topic><topic>Vaccines, DNA - immunology</topic><topic>Vagina - immunology</topic><topic>Vagina - microbiology</topic><topic>vaginal mucosa</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schautteet, K</creatorcontrib><creatorcontrib>Stuyven, E</creatorcontrib><creatorcontrib>Beeckman, D.S.A</creatorcontrib><creatorcontrib>Van Acker, S</creatorcontrib><creatorcontrib>Carlon, M</creatorcontrib><creatorcontrib>Chiers, K</creatorcontrib><creatorcontrib>Cox, E</creatorcontrib><creatorcontrib>Vanrompay, D</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nursing & Allied Health Database</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Healthcare Administration Database (Alumni)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Healthcare Administration Database</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schautteet, K</au><au>Stuyven, E</au><au>Beeckman, D.S.A</au><au>Van Acker, S</au><au>Carlon, M</au><au>Chiers, K</au><au>Cox, E</au><au>Vanrompay, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protection of pigs against Chlamydia trachomatis challenge by administration of a MOMP-based DNA vaccine in the vaginal mucosa</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2011-02-04</date><risdate>2011</risdate><volume>29</volume><issue>7</issue><spage>1399</spage><epage>1407</epage><pages>1399-1407</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Abstract Plasmid DNA (pWRG7079::MOMP) expressing the major outer membrane protein of a human Chlamydia trachomatis serovar E strain was tested for the ability to induce an immune response and protect against experimental genital infection with the same serovar. The vaccine was tested in pigs, as they are genetically and physiologically related to humans and suitable for studying C. trachomatis infection of the genital system. To increase the immune response, GM-CSF, LTA and B and CpG motives were used as adjuvants. GM-CSF was administered seven days before immunization, while the other adjuvants were administered together with the vaccine. Ten pigs were randomly divided into two groups. One group received an intravaginal primo-vaccination and a booster of 500 μg pWRG7079::MOMP, while the other group received the placebo vaccine pWRG7079. All animals were challenged with 108 TCID50 of C. trachomatis serovar E. Pigs immunized with the DNA vaccine showed significantly less macroscopic lesions, vaginal excretion and chlamydial replication in the genital tract, as compared to placebo-vaccinated controls. However, infection could not be completely cleared.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>21195805</pmid><doi>10.1016/j.vaccine.2010.12.042</doi><tpages>9</tpages></addata></record> |
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| subjects | adjuvants Adjuvants, Immunologic - administration & dosage Administration, Intravaginal Allergy and Immunology Animals Antibodies, Bacterial - blood Antibody Specificity Applied microbiology Bacterial Outer Membrane Proteins - administration & dosage Bacterial Outer Membrane Proteins - immunology Bacterial Shedding Bacterial Vaccines - administration & dosage Bacterial Vaccines - immunology Bacteriology Biological and medical sciences Cell Proliferation Chlamydia Infections - immunology Chlamydia Infections - prevention & control Chlamydia trachomatis Chlamydia trachomatis - immunology Cytokines - immunology Deoxyribonucleic acid DNA excretion Female Fundamental and applied biological sciences. Psychology Genital infection granulocyte-macrophage colony-stimulating factor humans Immune response Immune system Immunization Infections Leukocytes, Mononuclear - immunology Lymphocytes Microbiology Miscellaneous outer membrane proteins Pigs plasmids Porins - administration & dosage Porins - immunology recombinant vaccines serotypes Sexually transmitted diseases STD Swine Vaccine Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, DNA - administration & dosage Vaccines, DNA - immunology Vagina - immunology Vagina - microbiology vaginal mucosa |
| title | Protection of pigs against Chlamydia trachomatis challenge by administration of a MOMP-based DNA vaccine in the vaginal mucosa |
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