Survival of Transplanted Rat Bone Marrow-Derived Osteogenic Stem Cells In Vivo
This study was designed to trace bone marrow-derived stromal cells (MSC) after implantation in an ectopic rat model of bone tissue engineering. MSC were isolated from adult donor rats, expanded, seeded on a hydroxyapatite/β-tricalcium phosphate bone graft substitute (Straumann ® BoneCeramic), and cu...
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| Veröffentlicht in: | Tissue engineering. Part A 2011-04, Vol.17 (7-8), p.1147-1156 |
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| creator | Zimmermann, Corinna E. Gierloff, Matthias Hedderich, Jürgen Açil, Yahya Wiltfang, Joerg Terheyden, Hendrik |
| description | This study was designed to trace bone marrow-derived stromal cells (MSC) after implantation in an ectopic rat model of bone tissue engineering. MSC were isolated from adult donor rats, expanded, seeded on a hydroxyapatite/β-tricalcium phosphate bone graft substitute (Straumann
®
BoneCeramic), and cultivated until confluent. Before subcutaneous implantation of seeded constructs and controls (unseeded bone graft substitute) in isogenic rats (
n
= 32), cells were labeled with the fluorescent dye carboxyfluoresceine-diacetate-succinimidyl-ester. Specimens were harvested at sacrifice on day 1, 3, 7, or 14 after implantation (
n
= 8 per group) and processed for histology (hematoxylin and eosin, CD68, 4′,6-diamidino-2-phenylindol). Carboxyfluoresceine-diacetate-succinimidyl-ester-labeled transplanted cells were quantified in decalcified sections (50 fields of view per specimen) at 488 nm. Over time, transplanted cells decreased in number from 31.3 ± 2.3 (day 1) to 9.2 ± 1.1 (day 3) and 0.3 ± 0.1 (day 7) (
p
|
| doi_str_mv | 10.1089/ten.tea.2009.0577 |
| format | Article |
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®
BoneCeramic), and cultivated until confluent. Before subcutaneous implantation of seeded constructs and controls (unseeded bone graft substitute) in isogenic rats (
n
= 32), cells were labeled with the fluorescent dye carboxyfluoresceine-diacetate-succinimidyl-ester. Specimens were harvested at sacrifice on day 1, 3, 7, or 14 after implantation (
n
= 8 per group) and processed for histology (hematoxylin and eosin, CD68, 4′,6-diamidino-2-phenylindol). Carboxyfluoresceine-diacetate-succinimidyl-ester-labeled transplanted cells were quantified in decalcified sections (50 fields of view per specimen) at 488 nm. Over time, transplanted cells decreased in number from 31.3 ± 2.3 (day 1) to 9.2 ± 1.1 (day 3) and 0.3 ± 0.1 (day 7) (
p
< 0.001). Fourteen days postimplantation MSC could no longer be identified. Additionally, starting on day 3 postimplantation, cellular disintegration was noted. Multinucleated giant cells were present in constructs and controls on day 7 and increased to day 14 postimplantation. These results indicate that ectopically transplanted MSC survive for a rather short time after implantation. Possible reasons for early cell death are discussed.</description><identifier>ISSN: 1937-3341</identifier><identifier>EISSN: 1937-335X</identifier><identifier>DOI: 10.1089/ten.tea.2009.0577</identifier><identifier>PMID: 21142699</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Animals ; Biocompatible Materials - chemistry ; Bone cells ; Bone marrow ; Bone Marrow Cells - cytology ; Bone Marrow Transplantation - methods ; Bone Substitutes - chemistry ; Calcium Phosphates - chemistry ; Cells ; Durapatite - chemistry ; Growth ; Male ; Original Articles ; Osteogenesis - physiology ; Rats ; Rodents ; Stem cells ; Stem Cells - cytology ; Stromal Cells - cytology ; Tissue engineering ; Tissue Engineering - methods ; Transplantation</subject><ispartof>Tissue engineering. Part A, 2011-04, Vol.17 (7-8), p.1147-1156</ispartof><rights>2011, Mary Ann Liebert, Inc.</rights><rights>COPYRIGHT 2011 Mary Ann Liebert, Inc.</rights><rights>(©) Copyright 2011, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-24a82db511e8e22728e3f014e25cb3c93e4ab6b997f394e36a337188d44bbe43</citedby><cites>FETCH-LOGICAL-c474t-24a82db511e8e22728e3f014e25cb3c93e4ab6b997f394e36a337188d44bbe43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21142699$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zimmermann, Corinna E.</creatorcontrib><creatorcontrib>Gierloff, Matthias</creatorcontrib><creatorcontrib>Hedderich, Jürgen</creatorcontrib><creatorcontrib>Açil, Yahya</creatorcontrib><creatorcontrib>Wiltfang, Joerg</creatorcontrib><creatorcontrib>Terheyden, Hendrik</creatorcontrib><title>Survival of Transplanted Rat Bone Marrow-Derived Osteogenic Stem Cells In Vivo</title><title>Tissue engineering. Part A</title><addtitle>Tissue Eng Part A</addtitle><description>This study was designed to trace bone marrow-derived stromal cells (MSC) after implantation in an ectopic rat model of bone tissue engineering. MSC were isolated from adult donor rats, expanded, seeded on a hydroxyapatite/β-tricalcium phosphate bone graft substitute (Straumann
®
BoneCeramic), and cultivated until confluent. Before subcutaneous implantation of seeded constructs and controls (unseeded bone graft substitute) in isogenic rats (
n
= 32), cells were labeled with the fluorescent dye carboxyfluoresceine-diacetate-succinimidyl-ester. Specimens were harvested at sacrifice on day 1, 3, 7, or 14 after implantation (
n
= 8 per group) and processed for histology (hematoxylin and eosin, CD68, 4′,6-diamidino-2-phenylindol). Carboxyfluoresceine-diacetate-succinimidyl-ester-labeled transplanted cells were quantified in decalcified sections (50 fields of view per specimen) at 488 nm. Over time, transplanted cells decreased in number from 31.3 ± 2.3 (day 1) to 9.2 ± 1.1 (day 3) and 0.3 ± 0.1 (day 7) (
p
< 0.001). Fourteen days postimplantation MSC could no longer be identified. Additionally, starting on day 3 postimplantation, cellular disintegration was noted. Multinucleated giant cells were present in constructs and controls on day 7 and increased to day 14 postimplantation. These results indicate that ectopically transplanted MSC survive for a rather short time after implantation. Possible reasons for early cell death are discussed.</description><subject>Animals</subject><subject>Biocompatible Materials - chemistry</subject><subject>Bone cells</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Transplantation - methods</subject><subject>Bone Substitutes - chemistry</subject><subject>Calcium Phosphates - chemistry</subject><subject>Cells</subject><subject>Durapatite - chemistry</subject><subject>Growth</subject><subject>Male</subject><subject>Original Articles</subject><subject>Osteogenesis - physiology</subject><subject>Rats</subject><subject>Rodents</subject><subject>Stem cells</subject><subject>Stem Cells - cytology</subject><subject>Stromal Cells - cytology</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><subject>Transplantation</subject><issn>1937-3341</issn><issn>1937-335X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkU9rFTEUxYMotlY_gBsJuuhqxvybSbKsz6qFasE-xF3IzNwpKTPJM8k88dub4dWCIijhknD5nZObHISeU1JTovTrDL7OYGtGiK5JI-UDdEw1lxXnzdeH92dBj9CTlG4JaUkr5WN0xCgVrNX6GH26XuLe7e2Ew4i30fq0m6zPMODPNuM3wQP-aGMM36u3EN2-9K9ShnAD3vX4OsOMNzBNCV94_MXtw1P0aLRTgmd3-wnavjvfbj5Ul1fvLzZnl1UvpMgVE1axoWsoBQWMSaaAj4QKYE3f8V5zELZrO63lyLUA3lrOJVVqEKLrQPATdHqw3cXwbYGUzexSXwaxHsKSjGqFblrJ-L_JRgkqW7J6vvyDvA1L9OUVK0SkIFwV6NUBurETGOfHkKPtV0tzxhquRKn10vovVFkDzK4vfzq60v9NQA-CPoaUIoxmF91s4w9DiVmjNiXqUtasUZs16qJ5cTfv0s0w3Ct-ZVsAeQDWtvV-ctBBzP9h_ROK7LVx</recordid><startdate>20110401</startdate><enddate>20110401</enddate><creator>Zimmermann, Corinna E.</creator><creator>Gierloff, Matthias</creator><creator>Hedderich, Jürgen</creator><creator>Açil, Yahya</creator><creator>Wiltfang, Joerg</creator><creator>Terheyden, Hendrik</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20110401</creationdate><title>Survival of Transplanted Rat Bone Marrow-Derived Osteogenic Stem Cells In Vivo</title><author>Zimmermann, Corinna E. ; Gierloff, Matthias ; Hedderich, Jürgen ; Açil, Yahya ; Wiltfang, Joerg ; Terheyden, Hendrik</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-24a82db511e8e22728e3f014e25cb3c93e4ab6b997f394e36a337188d44bbe43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biocompatible Materials - chemistry</topic><topic>Bone cells</topic><topic>Bone marrow</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone Marrow Transplantation - methods</topic><topic>Bone Substitutes - chemistry</topic><topic>Calcium Phosphates - chemistry</topic><topic>Cells</topic><topic>Durapatite - chemistry</topic><topic>Growth</topic><topic>Male</topic><topic>Original Articles</topic><topic>Osteogenesis - physiology</topic><topic>Rats</topic><topic>Rodents</topic><topic>Stem cells</topic><topic>Stem Cells - cytology</topic><topic>Stromal Cells - cytology</topic><topic>Tissue engineering</topic><topic>Tissue Engineering - methods</topic><topic>Transplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zimmermann, Corinna E.</creatorcontrib><creatorcontrib>Gierloff, Matthias</creatorcontrib><creatorcontrib>Hedderich, Jürgen</creatorcontrib><creatorcontrib>Açil, Yahya</creatorcontrib><creatorcontrib>Wiltfang, Joerg</creatorcontrib><creatorcontrib>Terheyden, Hendrik</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Tissue engineering. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zimmermann, Corinna E.</au><au>Gierloff, Matthias</au><au>Hedderich, Jürgen</au><au>Açil, Yahya</au><au>Wiltfang, Joerg</au><au>Terheyden, Hendrik</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Survival of Transplanted Rat Bone Marrow-Derived Osteogenic Stem Cells In Vivo</atitle><jtitle>Tissue engineering. Part A</jtitle><addtitle>Tissue Eng Part A</addtitle><date>2011-04-01</date><risdate>2011</risdate><volume>17</volume><issue>7-8</issue><spage>1147</spage><epage>1156</epage><pages>1147-1156</pages><issn>1937-3341</issn><eissn>1937-335X</eissn><abstract>This study was designed to trace bone marrow-derived stromal cells (MSC) after implantation in an ectopic rat model of bone tissue engineering. MSC were isolated from adult donor rats, expanded, seeded on a hydroxyapatite/β-tricalcium phosphate bone graft substitute (Straumann
®
BoneCeramic), and cultivated until confluent. Before subcutaneous implantation of seeded constructs and controls (unseeded bone graft substitute) in isogenic rats (
n
= 32), cells were labeled with the fluorescent dye carboxyfluoresceine-diacetate-succinimidyl-ester. Specimens were harvested at sacrifice on day 1, 3, 7, or 14 after implantation (
n
= 8 per group) and processed for histology (hematoxylin and eosin, CD68, 4′,6-diamidino-2-phenylindol). Carboxyfluoresceine-diacetate-succinimidyl-ester-labeled transplanted cells were quantified in decalcified sections (50 fields of view per specimen) at 488 nm. Over time, transplanted cells decreased in number from 31.3 ± 2.3 (day 1) to 9.2 ± 1.1 (day 3) and 0.3 ± 0.1 (day 7) (
p
< 0.001). Fourteen days postimplantation MSC could no longer be identified. Additionally, starting on day 3 postimplantation, cellular disintegration was noted. Multinucleated giant cells were present in constructs and controls on day 7 and increased to day 14 postimplantation. These results indicate that ectopically transplanted MSC survive for a rather short time after implantation. Possible reasons for early cell death are discussed.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>21142699</pmid><doi>10.1089/ten.tea.2009.0577</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1937-3341 |
| ispartof | Tissue engineering. Part A, 2011-04, Vol.17 (7-8), p.1147-1156 |
| issn | 1937-3341 1937-335X |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Biocompatible Materials - chemistry Bone cells Bone marrow Bone Marrow Cells - cytology Bone Marrow Transplantation - methods Bone Substitutes - chemistry Calcium Phosphates - chemistry Cells Durapatite - chemistry Growth Male Original Articles Osteogenesis - physiology Rats Rodents Stem cells Stem Cells - cytology Stromal Cells - cytology Tissue engineering Tissue Engineering - methods Transplantation |
| title | Survival of Transplanted Rat Bone Marrow-Derived Osteogenic Stem Cells In Vivo |
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