Rapid movement of axonal neurofilaments interrupted by prolonged pauses
Axonal cytoskeletal and cytosolic proteins are synthesized in the neuronal cell body and transported along axons by slow axonal transport, but attempts to observe this movement directly in living cells have yielded conflicting results. Here we report the direct observation of the axonal transport of...
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| Veröffentlicht in: | Nature cell biology 2000-03, Vol.2 (3), p.137-141 |
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| creator | Wang, Lei Ho, Chung-liang Sun, Dongming Liem, Ronald K.H. Brown, Anthony |
| description | Axonal cytoskeletal and cytosolic proteins are synthesized in the neuronal cell body and transported along axons by slow axonal transport, but attempts to observe this movement directly in living cells have yielded conflicting results. Here we report the direct observation of the axonal transport of neurofilament protein tagged with green fluorescent protein in cultured nerve cells. Live-cell imaging of naturally occurring gaps in the axonal neurofilament array reveals rapid, intermittent and highly asynchronous movement of fluorescent neurofilaments. The movement is bidirectional, but predominantly anterograde. Our data indicate that the slow rate of slow axonal transport may be the result of rapid movements interrupted by prolonged pauses. |
| doi_str_mv | 10.1038/35004008 |
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Here we report the direct observation of the axonal transport of neurofilament protein tagged with green fluorescent protein in cultured nerve cells. Live-cell imaging of naturally occurring gaps in the axonal neurofilament array reveals rapid, intermittent and highly asynchronous movement of fluorescent neurofilaments. The movement is bidirectional, but predominantly anterograde. Our data indicate that the slow rate of slow axonal transport may be the result of rapid movements interrupted by prolonged pauses.</description><identifier>ISSN: 1465-7392</identifier><identifier>ISSN: 1476-4679</identifier><identifier>EISSN: 1476-4679</identifier><identifier>DOI: 10.1038/35004008</identifier><identifier>PMID: 10707083</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animals ; Animals, Newborn ; Axonal transport ; Axonal Transport - physiology ; Axons - metabolism ; Axons - ultrastructure ; Biological Transport - physiology ; Biomedical and Life Sciences ; Cancer Research ; Cell Biology ; Cells, Cultured ; Cytoplasmic filaments ; Developmental Biology ; Gene Expression ; Green Fluorescent Proteins ; Hypotheses ; Life Sciences ; Luminescent Proteins - genetics ; Microinjections ; Microscopy, Interference - methods ; Neurofilament Proteins - genetics ; Neurofilament Proteins - metabolism ; Neurons - cytology ; Neurons - metabolism ; Physiological aspects ; Polymers ; Proteins ; Rats ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; Stem Cells ; Superior Cervical Ganglion - cytology ; Time Factors ; Transfection</subject><ispartof>Nature cell biology, 2000-03, Vol.2 (3), p.137-141</ispartof><rights>Macmillan Magazines Ltd. 2000</rights><rights>COPYRIGHT 2000 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Mar 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-9fdab19f66d4edfa18625d6746093f52f55160533e2fb9bea1ee9b2df7635b513</citedby><cites>FETCH-LOGICAL-c476t-9fdab19f66d4edfa18625d6746093f52f55160533e2fb9bea1ee9b2df7635b513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/35004008$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/35004008$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10707083$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Lei</creatorcontrib><creatorcontrib>Ho, Chung-liang</creatorcontrib><creatorcontrib>Sun, Dongming</creatorcontrib><creatorcontrib>Liem, Ronald K.H.</creatorcontrib><creatorcontrib>Brown, Anthony</creatorcontrib><title>Rapid movement of axonal neurofilaments interrupted by prolonged pauses</title><title>Nature cell biology</title><addtitle>Nat Cell Biol</addtitle><addtitle>Nat Cell Biol</addtitle><description>Axonal cytoskeletal and cytosolic proteins are synthesized in the neuronal cell body and transported along axons by slow axonal transport, but attempts to observe this movement directly in living cells have yielded conflicting results. Here we report the direct observation of the axonal transport of neurofilament protein tagged with green fluorescent protein in cultured nerve cells. Live-cell imaging of naturally occurring gaps in the axonal neurofilament array reveals rapid, intermittent and highly asynchronous movement of fluorescent neurofilaments. The movement is bidirectional, but predominantly anterograde. Our data indicate that the slow rate of slow axonal transport may be the result of rapid movements interrupted by prolonged pauses.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Axonal transport</subject><subject>Axonal Transport - physiology</subject><subject>Axons - metabolism</subject><subject>Axons - ultrastructure</subject><subject>Biological Transport - physiology</subject><subject>Biomedical and Life Sciences</subject><subject>Cancer Research</subject><subject>Cell Biology</subject><subject>Cells, Cultured</subject><subject>Cytoplasmic filaments</subject><subject>Developmental Biology</subject><subject>Gene Expression</subject><subject>Green Fluorescent Proteins</subject><subject>Hypotheses</subject><subject>Life Sciences</subject><subject>Luminescent Proteins - genetics</subject><subject>Microinjections</subject><subject>Microscopy, Interference - methods</subject><subject>Neurofilament Proteins - genetics</subject><subject>Neurofilament Proteins - metabolism</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>Physiological aspects</subject><subject>Polymers</subject><subject>Proteins</subject><subject>Rats</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Stem Cells</subject><subject>Superior Cervical Ganglion - cytology</subject><subject>Time Factors</subject><subject>Transfection</subject><issn>1465-7392</issn><issn>1476-4679</issn><issn>1476-4679</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkstu1TAQhi0EoqUg8QQoYsFlkTK-xl5WFZRKlZAKrC0nHh-lSuJgJ6h9exxOUSlI4Fn49nnm98wQ8pzCMQWu33EJIAD0A3JIRaNqoRrzcFsrWTfcsAPyJOcrACoENI_JAYWmmOaH5OzSzb2vxvgdR5yWKobKXcfJDdWEa4qhH9x2nqt-WjCldV7QV-1NNac4xGlXNrNbM-an5FFwQ8Znt_MR-frh_ZfTj_XFp7Pz05OLuiu6ltoE71pqglJeoA-OasWkV41QYHiQLEhJFUjOkYXWtOgoommZD43ispWUH5HXe79FwLcV82LHPnc4DG7CuGarlTCSUcEL-eqfZANGasPMf0GqpZYCttgv_wCv4ppKrrJlZWgAtkHHe2jnBrT9FOKSXFfM49h3ccKSUrQnVHOmmfyp8-29B4VZ8HrZlbRme_758j77Zs92KeacMNg59aNLN5aC3TrB_uqEgr64Fbu2I_rfwH3p7-LmclUqme5-85ezH6BRt9o</recordid><startdate>20000301</startdate><enddate>20000301</enddate><creator>Wang, Lei</creator><creator>Ho, Chung-liang</creator><creator>Sun, Dongming</creator><creator>Liem, Ronald K.H.</creator><creator>Brown, Anthony</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20000301</creationdate><title>Rapid movement of axonal neurofilaments interrupted by prolonged pauses</title><author>Wang, Lei ; Ho, Chung-liang ; Sun, Dongming ; Liem, Ronald K.H. ; Brown, Anthony</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-9fdab19f66d4edfa18625d6746093f52f55160533e2fb9bea1ee9b2df7635b513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Axonal transport</topic><topic>Axonal Transport - 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cytology</topic><topic>Time Factors</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Lei</creatorcontrib><creatorcontrib>Ho, Chung-liang</creatorcontrib><creatorcontrib>Sun, Dongming</creatorcontrib><creatorcontrib>Liem, Ronald K.H.</creatorcontrib><creatorcontrib>Brown, Anthony</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Nature cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Lei</au><au>Ho, Chung-liang</au><au>Sun, Dongming</au><au>Liem, Ronald K.H.</au><au>Brown, Anthony</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid movement of axonal neurofilaments interrupted by prolonged pauses</atitle><jtitle>Nature cell biology</jtitle><stitle>Nat Cell Biol</stitle><addtitle>Nat Cell Biol</addtitle><date>2000-03-01</date><risdate>2000</risdate><volume>2</volume><issue>3</issue><spage>137</spage><epage>141</epage><pages>137-141</pages><issn>1465-7392</issn><issn>1476-4679</issn><eissn>1476-4679</eissn><abstract>Axonal cytoskeletal and cytosolic proteins are synthesized in the neuronal cell body and transported along axons by slow axonal transport, but attempts to observe this movement directly in living cells have yielded conflicting results. Here we report the direct observation of the axonal transport of neurofilament protein tagged with green fluorescent protein in cultured nerve cells. Live-cell imaging of naturally occurring gaps in the axonal neurofilament array reveals rapid, intermittent and highly asynchronous movement of fluorescent neurofilaments. The movement is bidirectional, but predominantly anterograde. Our data indicate that the slow rate of slow axonal transport may be the result of rapid movements interrupted by prolonged pauses.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>10707083</pmid><doi>10.1038/35004008</doi><tpages>5</tpages></addata></record> |
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| ispartof | Nature cell biology, 2000-03, Vol.2 (3), p.137-141 |
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| subjects | Animals Animals, Newborn Axonal transport Axonal Transport - physiology Axons - metabolism Axons - ultrastructure Biological Transport - physiology Biomedical and Life Sciences Cancer Research Cell Biology Cells, Cultured Cytoplasmic filaments Developmental Biology Gene Expression Green Fluorescent Proteins Hypotheses Life Sciences Luminescent Proteins - genetics Microinjections Microscopy, Interference - methods Neurofilament Proteins - genetics Neurofilament Proteins - metabolism Neurons - cytology Neurons - metabolism Physiological aspects Polymers Proteins Rats Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Stem Cells Superior Cervical Ganglion - cytology Time Factors Transfection |
| title | Rapid movement of axonal neurofilaments interrupted by prolonged pauses |
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