Preparation of a Lactobacillus Plantarum Starter Culture for Cucumber Fermentations That Can Meet Kosher Guidelines

:  A method is described for growth of a Lactobacillus plantarum starter culture in jars of commercially available pasteurized fresh‐pack kosher dill cucumbers so that jars can be used to inoculate commercial scale cucumber fermentation tanks. A procedure is also described to transfer lactic acid ba...

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Veröffentlicht in:Journal of food science 2011-03, Vol.76 (2), p.M120-M123
Hauptverfasser: Pérez-Díaz, I.M., McFeeters, R.F.
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container_title Journal of food science
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creator Pérez-Díaz, I.M.
McFeeters, R.F.
description :  A method is described for growth of a Lactobacillus plantarum starter culture in jars of commercially available pasteurized fresh‐pack kosher dill cucumbers so that jars can be used to inoculate commercial scale cucumber fermentation tanks. A procedure is also described to transfer lactic acid bacteria from frozen storage in MRS broth into cucumber juice and commercial jars of kosher dill cucumbers so that a selected strain of lactic acid bacteria can be kosher certified for commercial fermentations in processing plants that operate under kosher certification. The strain of L. plantarum used in these experiments grew to maximum cell numbers in 4 d at 20 to 25 °C and then maintained viable cell numbers for 2 wk at >108 CFU/mL so the culture was suitable for inoculation of fermentation tanks. Refrigeration of jars of culture after they grow to maximum numbers minimizes die‐off of cells sufficiently so that a pure culture can be maintained by aseptically transferring brine containing viable bacteria to a new pH‐adjusted jar only once every 4 mo. Practical Application:  This report describes a method to prepare a lactic acid bacteria starter culture suitable for kosher vegetable fermentations.
doi_str_mv 10.1111/j.1750-3841.2010.01981.x
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subjects Biological and medical sciences
Cell culture
Cell growth
Colony Count, Microbial
Cucumbers
Cucumis sativus - microbiology
Culture Media
Fermentation
Food Handling - methods
Food Handling - standards
Food industries
Food microbiology
Food Microbiology - standards
Food science
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
Gram-positive bacteria
Hydrogen-Ion Concentration
lactic acid bacteria
Lactobacillus
Lactobacillus plantarum - growth & development
Pasteurization
Refrigeration
Salts - chemistry
Salts - metabolism
starter culture
Vegetables - microbiology
title Preparation of a Lactobacillus Plantarum Starter Culture for Cucumber Fermentations That Can Meet Kosher Guidelines
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