Simultaneous immobilization of glucose oxidase on the surface and cavity of hollow gold nanospheres as labels for highly sensitive electrochemical immunoassay of tumor marker

A novel tracer, glucose oxidase (GOD)-functionalized hollow gold nanospheres encapsulating glucose oxidase (Au shell@GOD), was designed to label the ferrocenemonocarboxylic-grafted secondary antibodies (Fc@Ab 2) for highly sensitive detection of tumor marker using carboxyl group functionalized multi...

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Veröffentlicht in:	Biosensors & bioelectronics 2011-01, Vol.26 (5), p.2776-2780
Hauptverfasser:	Song, Zhongju, Yuan, Ruo, Chai, Yaqin, Jiang, Wen, Su, Huilan, Che, Xin, Ran, Xiaoqi
Format:	Artikel
Sprache:	eng
Schlagworte:	Amperometric immunosensor antibodies antigens Biological and medical sciences biomarkers Biomarkers, Tumor - blood Biomarkers, Tumor - immunology Biosensing Techniques - instrumentation Biosensors Biotechnology blood serum carbon nanotubes Carcinoembryonic Antigen - blood Co-immobilization Conductometry - instrumentation detection limit electrochemistry electrodes electron transfer encapsulation Enzymes, Immobilized - chemistry Equipment Design Equipment Failure Analysis Fundamental and applied biological sciences. Psychology General aspects glucose oxidase Glucose Oxidase - chemistry Glucose Oxidase - immunology Glucose oxidase entrapment gold Gold - chemistry Hollow gold nanospheres Humans Immobilization techniques Immunoassay - instrumentation immunoassays Methods. Procedures. Technologies micelles Multiwall carbon nanotubes (MWCNTs) nanospheres Nanospheres - chemistry neoplasms normal values screening Staining and Labeling - methods Surface Properties Various methods and equipments
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