Localization of eNOS in the Olfactory Epithelium of the Rat
Nitric oxide (NO) is a free radical and produced from L-arginine by nitric oxide synthase (NOS). Since NO is recently suggested to be involved in olfactory perception, the expression of eNOS, an isoform of NOS, was examined in the rat olfactory epithelium. The activity of NADPH-diaphorase was also e...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2011, Vol.73(4), pp.423-430 |
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| container_title | Journal of Veterinary Medical Science |
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| creator | ENDO, Daisuke YAMAMOTO, Yoshio YAMAGUCHI-YAMADA, Misuzu NAKAMUTA, Nobuaki TANIGUCHI, Kazuyuki |
| description | Nitric oxide (NO) is a free radical and produced from L-arginine by nitric oxide synthase (NOS). Since NO is recently suggested to be involved in olfactory perception, the expression of eNOS, an isoform of NOS, was examined in the rat olfactory epithelium. The activity of NADPH-diaphorase was also examined as a marker of NOS. In the dorsomedial region of the nasal cavity, intensely positive reactions for NADPH-diaphorase were observed in the entire cytoplasm of sensory cells (olfactory cells). By immunohistochemistry, intensely positive reactions for eNOS were also found in the dorsomedial region of the nasal cavity. These reactions were observed on the free border of the olfactory epithelium. By immunoelectron microscopy, positive reactions for eNOS were found in the cilia of olfactory cells. In addition, in situ hybridization analysis of the olfactory epithelium revealed the expression of eNOS mRNA in the olfactory cells. These results indicate the presence of eNOS in the olfactory cells of the rat, and differential expression of eNOS in the olfactory epithelium depending on the regions of the nasal cavity. In addition, NO produced by eNOS may be involved in olfactory perception in the cilia of olfactory cells. |
| doi_str_mv | 10.1292/jvms.10-0353 |
| format | Article |
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Since NO is recently suggested to be involved in olfactory perception, the expression of eNOS, an isoform of NOS, was examined in the rat olfactory epithelium. The activity of NADPH-diaphorase was also examined as a marker of NOS. In the dorsomedial region of the nasal cavity, intensely positive reactions for NADPH-diaphorase were observed in the entire cytoplasm of sensory cells (olfactory cells). By immunohistochemistry, intensely positive reactions for eNOS were also found in the dorsomedial region of the nasal cavity. These reactions were observed on the free border of the olfactory epithelium. By immunoelectron microscopy, positive reactions for eNOS were found in the cilia of olfactory cells. In addition, in situ hybridization analysis of the olfactory epithelium revealed the expression of eNOS mRNA in the olfactory cells. These results indicate the presence of eNOS in the olfactory cells of the rat, and differential expression of eNOS in the olfactory epithelium depending on the regions of the nasal cavity. In addition, NO produced by eNOS may be involved in olfactory perception in the cilia of olfactory cells.</description><identifier>ISSN: 0916-7250</identifier><identifier>ISSN: 1347-7439</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.10-0353</identifier><identifier>PMID: 21068516</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Animals ; eNOS ; Gene Expression Regulation, Enzymologic - physiology ; Male ; NADH Dehydrogenase - genetics ; NADH Dehydrogenase - metabolism ; NADPH-diaphorase ; nitric oxide ; Nitric Oxide Synthase Type III - metabolism ; olfactory epithelium ; Olfactory Mucosa - cytology ; Olfactory Mucosa - enzymology ; Olfactory Mucosa - metabolism ; rat ; Rats ; Rats, Wistar</subject><ispartof>Journal of Veterinary Medical Science, 2011, Vol.73(4), pp.423-430</ispartof><rights>2011 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c573t-ec22e70735974b7bd19153f4f9d03e89b0ed4d410f8eab265e6e1054158d1cab3</citedby><cites>FETCH-LOGICAL-c573t-ec22e70735974b7bd19153f4f9d03e89b0ed4d410f8eab265e6e1054158d1cab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21068516$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ENDO, Daisuke</creatorcontrib><creatorcontrib>YAMAMOTO, Yoshio</creatorcontrib><creatorcontrib>YAMAGUCHI-YAMADA, Misuzu</creatorcontrib><creatorcontrib>NAKAMUTA, Nobuaki</creatorcontrib><creatorcontrib>TANIGUCHI, Kazuyuki</creatorcontrib><title>Localization of eNOS in the Olfactory Epithelium of the Rat</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>Nitric oxide (NO) is a free radical and produced from L-arginine by nitric oxide synthase (NOS). Since NO is recently suggested to be involved in olfactory perception, the expression of eNOS, an isoform of NOS, was examined in the rat olfactory epithelium. The activity of NADPH-diaphorase was also examined as a marker of NOS. In the dorsomedial region of the nasal cavity, intensely positive reactions for NADPH-diaphorase were observed in the entire cytoplasm of sensory cells (olfactory cells). By immunohistochemistry, intensely positive reactions for eNOS were also found in the dorsomedial region of the nasal cavity. These reactions were observed on the free border of the olfactory epithelium. By immunoelectron microscopy, positive reactions for eNOS were found in the cilia of olfactory cells. In addition, in situ hybridization analysis of the olfactory epithelium revealed the expression of eNOS mRNA in the olfactory cells. These results indicate the presence of eNOS in the olfactory cells of the rat, and differential expression of eNOS in the olfactory epithelium depending on the regions of the nasal cavity. In addition, NO produced by eNOS may be involved in olfactory perception in the cilia of olfactory cells.</description><subject>Animals</subject><subject>eNOS</subject><subject>Gene Expression Regulation, Enzymologic - physiology</subject><subject>Male</subject><subject>NADH Dehydrogenase - genetics</subject><subject>NADH Dehydrogenase - metabolism</subject><subject>NADPH-diaphorase</subject><subject>nitric oxide</subject><subject>Nitric Oxide Synthase Type III - metabolism</subject><subject>olfactory epithelium</subject><subject>Olfactory Mucosa - cytology</subject><subject>Olfactory Mucosa - enzymology</subject><subject>Olfactory Mucosa - metabolism</subject><subject>rat</subject><subject>Rats</subject><subject>Rats, Wistar</subject><issn>0916-7250</issn><issn>1347-7439</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90Mtv1DAQBnALFdGl9MYZReoBDqSd8SO21RNU5SGtuhLQc-Q4E5pVHls7QSp_PY522QMHLrbs-emT5mPsNcIlcsuvtr_6eImQg1DiGVuhkDrXUtgTtgKLRa65glP2MsYtAEdZ2BfslCMURmGxYtfr0buu_e2mdhyyscnobvM9a4dseqBs0zXOT2N4ym53bfro2rlfzDL75qZX7Hnjukjnh_uM3X-6_XHzJV9vPn-9-bDOvdJiyslzThq0UFbLSlc1WlSikY2tQZCxFVAta4nQGHIVLxQVhKAkKlOjd5U4Y2_3ubswPs4Up7Jvo6eucwONcyxNIVMkCEzy3X8lAhgjhEWd6MU_dDvOYUh7lKkkozkYCUm93ysfxhgDNeUutL0LTymqXOovl_qXx1J_4m8OoXPVU33Ef_tO4OMebOPkftIRuDC1vqN9mhalXI5D6nHoH1woaRB_AG0ylaw</recordid><startdate>20110401</startdate><enddate>20110401</enddate><creator>ENDO, Daisuke</creator><creator>YAMAMOTO, Yoshio</creator><creator>YAMAGUCHI-YAMADA, Misuzu</creator><creator>NAKAMUTA, Nobuaki</creator><creator>TANIGUCHI, Kazuyuki</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20110401</creationdate><title>Localization of eNOS in the Olfactory Epithelium of the Rat</title><author>ENDO, Daisuke ; YAMAMOTO, Yoshio ; YAMAGUCHI-YAMADA, Misuzu ; NAKAMUTA, Nobuaki ; TANIGUCHI, Kazuyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c573t-ec22e70735974b7bd19153f4f9d03e89b0ed4d410f8eab265e6e1054158d1cab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>eNOS</topic><topic>Gene Expression Regulation, Enzymologic - physiology</topic><topic>Male</topic><topic>NADH Dehydrogenase - genetics</topic><topic>NADH Dehydrogenase - metabolism</topic><topic>NADPH-diaphorase</topic><topic>nitric oxide</topic><topic>Nitric Oxide Synthase Type III - metabolism</topic><topic>olfactory epithelium</topic><topic>Olfactory Mucosa - cytology</topic><topic>Olfactory Mucosa - enzymology</topic><topic>Olfactory Mucosa - metabolism</topic><topic>rat</topic><topic>Rats</topic><topic>Rats, Wistar</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ENDO, Daisuke</creatorcontrib><creatorcontrib>YAMAMOTO, Yoshio</creatorcontrib><creatorcontrib>YAMAGUCHI-YAMADA, Misuzu</creatorcontrib><creatorcontrib>NAKAMUTA, Nobuaki</creatorcontrib><creatorcontrib>TANIGUCHI, Kazuyuki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ENDO, Daisuke</au><au>YAMAMOTO, Yoshio</au><au>YAMAGUCHI-YAMADA, Misuzu</au><au>NAKAMUTA, Nobuaki</au><au>TANIGUCHI, Kazuyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localization of eNOS in the Olfactory Epithelium of the Rat</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2011-04-01</date><risdate>2011</risdate><volume>73</volume><issue>4</issue><spage>423</spage><epage>430</epage><pages>423-430</pages><issn>0916-7250</issn><issn>1347-7439</issn><eissn>1347-7439</eissn><abstract>Nitric oxide (NO) is a free radical and produced from L-arginine by nitric oxide synthase (NOS). Since NO is recently suggested to be involved in olfactory perception, the expression of eNOS, an isoform of NOS, was examined in the rat olfactory epithelium. The activity of NADPH-diaphorase was also examined as a marker of NOS. In the dorsomedial region of the nasal cavity, intensely positive reactions for NADPH-diaphorase were observed in the entire cytoplasm of sensory cells (olfactory cells). By immunohistochemistry, intensely positive reactions for eNOS were also found in the dorsomedial region of the nasal cavity. These reactions were observed on the free border of the olfactory epithelium. 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| subjects | Animals eNOS Gene Expression Regulation, Enzymologic - physiology Male NADH Dehydrogenase - genetics NADH Dehydrogenase - metabolism NADPH-diaphorase nitric oxide Nitric Oxide Synthase Type III - metabolism olfactory epithelium Olfactory Mucosa - cytology Olfactory Mucosa - enzymology Olfactory Mucosa - metabolism rat Rats Rats, Wistar |
| title | Localization of eNOS in the Olfactory Epithelium of the Rat |
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