Real-Time Measurements of Protein Dynamics Using Fluorescence Activation-Coupled Protein Labeling Method

We present a fluorescence activation-coupled protein labeling (FAPL) method, which employs small-molecular probes that exhibit almost no basal fluorescence but acquire strong fluorescence upon covalent binding to tag-proteins. This method enables real-time imaging of protein labeling without any was...

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Veröffentlicht in:	Journal of the American Chemical Society 2011-05, Vol.133 (17), p.6745-6751
Hauptverfasser:	Komatsu, Toru, Johnsson, Kai, Okuno, Hiroyuki, Bito, Haruhiko, Inoue, Takanari, Nagano, Tetsuo, Urano, Yasuteru
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Line Cell Movement Cercopithecus aethiops COS Cells Fluorescent Dyes - chemistry Molecular Probe Techniques Molecular Probes - chemistry Proteins - analysis Receptor, Epidermal Growth Factor - analysis
Online-Zugang:	Volltext
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