Frequency of DLK1 c.639C>T polymorphism and the analysis of MEG3/DLK1/PEG11 cluster expression in muscle of swine raised in Poland
DLK1 — (Drosophila like element 1) is a paternally expressed gene, associated with the callipyge phenotype in sheep. In a present study we designed a new real-time PCR alleleic discrimination assay for genotyping of a silent C/T mutation (c.639C>T) in DLK1 gene in swine. The DLK1 c.639C>T muta...
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| Veröffentlicht in: | Meat science 2011-08, Vol.88 (4), p.627-630 |
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| creator | Oczkowicz, M. Ropka-Molik, K. Piórkowska, K. Różycki, M. Rejduch, B. |
| description | DLK1 — (Drosophila like element 1) is a paternally expressed gene, associated with the callipyge phenotype in sheep. In a present study we designed a new real-time PCR alleleic discrimination assay for genotyping of a silent C/T mutation (c.639C>T) in
DLK1 gene in swine. The
DLK1 c.639C>T mutation was highly polymorphic in all breeds analyzed and C allele was predominant in Landrace and Duroc while T allele was more frequent in Pietrain and Puławska breed. Moreover, we analyzed mRNA expression of
DLK1 and adjacent genes —
MEG3 and
PEG11 in muscles of swines of different breeds raised in Poland. We did not observe significantly different expression of
DLK1,
MEG3 or
PEG11 mRNA in any of analyzed breeds. We also attempted to assess the effect of
DLK1 (c.639C>T) on the expression of genes in callipyge locus but did not find significant differences between animals with alternate genotypes (C/C and T/T homozygotes). |
| doi_str_mv | 10.1016/j.meatsci.2011.02.019 |
| format | Article |
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DLK1 gene in swine. The
DLK1 c.639C>T mutation was highly polymorphic in all breeds analyzed and C allele was predominant in Landrace and Duroc while T allele was more frequent in Pietrain and Puławska breed. Moreover, we analyzed mRNA expression of
DLK1 and adjacent genes —
MEG3 and
PEG11 in muscles of swines of different breeds raised in Poland. We did not observe significantly different expression of
DLK1,
MEG3 or
PEG11 mRNA in any of analyzed breeds. We also attempted to assess the effect of
DLK1 (c.639C>T) on the expression of genes in callipyge locus but did not find significant differences between animals with alternate genotypes (C/C and T/T homozygotes).</description><identifier>ISSN: 0309-1740</identifier><identifier>EISSN: 1873-4138</identifier><identifier>DOI: 10.1016/j.meatsci.2011.02.019</identifier><identifier>PMID: 21414727</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Alleles ; Animals ; Callipyge ; DLK1 ; Gene Expression ; Gene Frequency ; Gene Silencing ; Homozygote ; Imprinting ; MEG3 ; Multigene Family ; Muscle Proteins - genetics ; Muscle Proteins - metabolism ; Muscles - metabolism ; Mutation ; PEG11 ; Phenotype ; Poland ; Polar overdominance ; Polymorphism, Genetic ; Swine</subject><ispartof>Meat science, 2011-08, Vol.88 (4), p.627-630</ispartof><rights>2011 Elsevier Ltd</rights><rights>Copyright © 2011 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c364t-db62d1464961e44748ece4b153c2dec7a45aa33a1138430083aa34f7d78dc4723</citedby><cites>FETCH-LOGICAL-c364t-db62d1464961e44748ece4b153c2dec7a45aa33a1138430083aa34f7d78dc4723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0309174011000763$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21414727$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oczkowicz, M.</creatorcontrib><creatorcontrib>Ropka-Molik, K.</creatorcontrib><creatorcontrib>Piórkowska, K.</creatorcontrib><creatorcontrib>Różycki, M.</creatorcontrib><creatorcontrib>Rejduch, B.</creatorcontrib><title>Frequency of DLK1 c.639C>T polymorphism and the analysis of MEG3/DLK1/PEG11 cluster expression in muscle of swine raised in Poland</title><title>Meat science</title><addtitle>Meat Sci</addtitle><description>DLK1 — (Drosophila like element 1) is a paternally expressed gene, associated with the callipyge phenotype in sheep. In a present study we designed a new real-time PCR alleleic discrimination assay for genotyping of a silent C/T mutation (c.639C>T) in
DLK1 gene in swine. The
DLK1 c.639C>T mutation was highly polymorphic in all breeds analyzed and C allele was predominant in Landrace and Duroc while T allele was more frequent in Pietrain and Puławska breed. Moreover, we analyzed mRNA expression of
DLK1 and adjacent genes —
MEG3 and
PEG11 in muscles of swines of different breeds raised in Poland. We did not observe significantly different expression of
DLK1,
MEG3 or
PEG11 mRNA in any of analyzed breeds. We also attempted to assess the effect of
DLK1 (c.639C>T) on the expression of genes in callipyge locus but did not find significant differences between animals with alternate genotypes (C/C and T/T homozygotes).</description><subject>Alleles</subject><subject>Animals</subject><subject>Callipyge</subject><subject>DLK1</subject><subject>Gene Expression</subject><subject>Gene Frequency</subject><subject>Gene Silencing</subject><subject>Homozygote</subject><subject>Imprinting</subject><subject>MEG3</subject><subject>Multigene Family</subject><subject>Muscle Proteins - genetics</subject><subject>Muscle Proteins - metabolism</subject><subject>Muscles - metabolism</subject><subject>Mutation</subject><subject>PEG11</subject><subject>Phenotype</subject><subject>Poland</subject><subject>Polar overdominance</subject><subject>Polymorphism, Genetic</subject><subject>Swine</subject><issn>0309-1740</issn><issn>1873-4138</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9vEzEQxa0K1KYtHwHkG6fdeGxnd3MBoZAGRFB7KGfLsSeqo_2HZ7eQK5-8XiVw5TSy_N6beT_G3oLIQUAxP-QN2oFcyKUAyIXMBSwv2AyqUmUaVPWKzYQSywxKLa7YNdFBCAFKVpfsSoIGXcpyxv7cRfw5YuuOvNvzz9tvwF1eqOXqwyPvu_rYdLF_CtRw23o-PGGatj5SoEn-fb1R88kzf1hvIDnrkQaMHH_3EYlC1_LQ8mYkV-Okp1-hRR5tIPTTz0NXp9hb9npva8I353nDftytH1dfsu395uvq0zZzqtBD5neF9KALvSwAtS51hQ71DhbKSY-utHphrVIWUnethKhUeup96cvKu1RW3bD3p9w-dqkyDaYJ5LBON2A3kqkKpZXUEpJycVK62BFF3Js-hsbGowFhJvrmYM70zUTfCGkS_eR7d94w7hr0_1x_cSfBx5MAU8_ngNGkiAQffYjoBuO78J8VL2melqI</recordid><startdate>20110801</startdate><enddate>20110801</enddate><creator>Oczkowicz, M.</creator><creator>Ropka-Molik, K.</creator><creator>Piórkowska, K.</creator><creator>Różycki, M.</creator><creator>Rejduch, B.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110801</creationdate><title>Frequency of DLK1 c.639C>T polymorphism and the analysis of MEG3/DLK1/PEG11 cluster expression in muscle of swine raised in Poland</title><author>Oczkowicz, M. ; Ropka-Molik, K. ; Piórkowska, K. ; Różycki, M. ; Rejduch, B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c364t-db62d1464961e44748ece4b153c2dec7a45aa33a1138430083aa34f7d78dc4723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Alleles</topic><topic>Animals</topic><topic>Callipyge</topic><topic>DLK1</topic><topic>Gene Expression</topic><topic>Gene Frequency</topic><topic>Gene Silencing</topic><topic>Homozygote</topic><topic>Imprinting</topic><topic>MEG3</topic><topic>Multigene Family</topic><topic>Muscle Proteins - genetics</topic><topic>Muscle Proteins - metabolism</topic><topic>Muscles - metabolism</topic><topic>Mutation</topic><topic>PEG11</topic><topic>Phenotype</topic><topic>Poland</topic><topic>Polar overdominance</topic><topic>Polymorphism, Genetic</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oczkowicz, M.</creatorcontrib><creatorcontrib>Ropka-Molik, K.</creatorcontrib><creatorcontrib>Piórkowska, K.</creatorcontrib><creatorcontrib>Różycki, M.</creatorcontrib><creatorcontrib>Rejduch, B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Meat science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oczkowicz, M.</au><au>Ropka-Molik, K.</au><au>Piórkowska, K.</au><au>Różycki, M.</au><au>Rejduch, B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Frequency of DLK1 c.639C>T polymorphism and the analysis of MEG3/DLK1/PEG11 cluster expression in muscle of swine raised in Poland</atitle><jtitle>Meat science</jtitle><addtitle>Meat Sci</addtitle><date>2011-08-01</date><risdate>2011</risdate><volume>88</volume><issue>4</issue><spage>627</spage><epage>630</epage><pages>627-630</pages><issn>0309-1740</issn><eissn>1873-4138</eissn><abstract>DLK1 — (Drosophila like element 1) is a paternally expressed gene, associated with the callipyge phenotype in sheep. In a present study we designed a new real-time PCR alleleic discrimination assay for genotyping of a silent C/T mutation (c.639C>T) in
DLK1 gene in swine. The
DLK1 c.639C>T mutation was highly polymorphic in all breeds analyzed and C allele was predominant in Landrace and Duroc while T allele was more frequent in Pietrain and Puławska breed. Moreover, we analyzed mRNA expression of
DLK1 and adjacent genes —
MEG3 and
PEG11 in muscles of swines of different breeds raised in Poland. We did not observe significantly different expression of
DLK1,
MEG3 or
PEG11 mRNA in any of analyzed breeds. We also attempted to assess the effect of
DLK1 (c.639C>T) on the expression of genes in callipyge locus but did not find significant differences between animals with alternate genotypes (C/C and T/T homozygotes).</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>21414727</pmid><doi>10.1016/j.meatsci.2011.02.019</doi><tpages>4</tpages></addata></record> |
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| ispartof | Meat science, 2011-08, Vol.88 (4), p.627-630 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Alleles Animals Callipyge DLK1 Gene Expression Gene Frequency Gene Silencing Homozygote Imprinting MEG3 Multigene Family Muscle Proteins - genetics Muscle Proteins - metabolism Muscles - metabolism Mutation PEG11 Phenotype Poland Polar overdominance Polymorphism, Genetic Swine |
| title | Frequency of DLK1 c.639C>T polymorphism and the analysis of MEG3/DLK1/PEG11 cluster expression in muscle of swine raised in Poland |
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