Metal complexes as artificial proteases in proteomics: A palladium(II) complex cleaves various proteins in solutions containing detergents

Most popular agents for site-specific protein cleavage are proteolytic enzymes. Because they become denatured and inactivated by detergents, enzymes are inconvenient for proteomic analysis of hydrophobic proteins which require detergents as solubilizing agents. We used cis-[Pd(en)(H 2O) 2] 2+ (in wh...

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Veröffentlicht in:	Journal of inorganic biochemistry 2011-05, Vol.105 (5), p.675-683
Hauptverfasser:	Miskevich, Frank, Davis, Amy, Leeprapaiwong, Porntip, Giganti, Virginia, Kostić, Nenad M., Angel, Laurence A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Caseins - metabolism Cattle Cholic Acids - chemistry Cholic Acids - metabolism Chromatography, High Pressure Liquid Coordination Complexes - chemistry Coordination Complexes - metabolism Detergents - chemistry Electrospray ionization Kinetics Mass spectrometry Membrane protein Molecular Sequence Data Palladium - chemistry Peptide Hydrolases - chemistry Peptide Hydrolases - metabolism Protein degradation Protein solubility Proteome Proteomics - methods Quaternary Ammonium Compounds - chemistry Quaternary Ammonium Compounds - metabolism Ubiquitin - metabolism
Online-Zugang:	Volltext
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