Serum total tryptase levels are increased in patients with active chronic urticaria
Summary Background We have demonstrated previously mast cell histamine release upon incubation with chronic urticaria (CU) sera, presumably by degranulation. Objective To explore total and mature tryptase in order to assess whether any increase in total tryptase levels is due in part to mast cell de...
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| Veröffentlicht in: | Clinical and experimental allergy 2010-12, Vol.40 (12), p.1760-1766 |
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| container_title | Clinical and experimental allergy |
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| creator | Ferrer, M. Nuñez-Córdoba, J. M. Luquin, E. Grattan, C. E. De la Borbolla, J. M. Sanz, M. L. Schwartz, L. B. |
| description | Summary
Background
We have demonstrated previously mast cell histamine release upon incubation with chronic urticaria (CU) sera, presumably by degranulation.
Objective
To explore total and mature tryptase in order to assess whether any increase in total tryptase levels is due in part to mast cell degranulation or to mast cell burden. We also wanted to explore differences between the autoimmune groups called idiopathic (serum unable to activate basophils), and to correlate total and mature tryptase levels with different urticaria features.
Methods
We measured total and mature tryptase serum levels in 81 CU patients, 16 atopic donors and 21 healthy control sera. We assessed autoimmunity by measuring the CD63 expression in normal basophil donors upon incubation with CU sera.
Results
We found significantly higher levels of total tryptase in the sera of CU patients (6.6 ±4.1 μg/L) than in sera from healthy non‐atopic subjects (4.4 ±2.8 μg/L) and from atopic subjects (4.5 ±1.7 μg/L). Mature tryptase levels were undetectable ( |
| doi_str_mv | 10.1111/j.1365-2222.2010.03582.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_862792748</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>862792748</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4962-5315e82fa7c430b3c14cde9dfee99385853431350a6ec4ee3981e5f146d509403</originalsourceid><addsrcrecordid>eNqNkU1vEzEQhi0EoqHwF5AlhHra4M-1feBQRSVBqsqhoB4t1zurOmx2g-1tk3-Pl4QgcYG5eDR-3pmxX4QwJXNa4sN6TnktK1ZizkipEi41m--eodnp4jmaESNFpbQRZ-hVSmtCCmb0S3TGiKJaaTpDt7cQxw3OQ3YdznG_zS4B7uARuoRdBBx6H6HUmpLhrcsB-pzwU8gP2PkcHgH7hzj0weMx5uBdDO41etG6LsGb43mOvn26-rpYVddflp8Xl9eVF6ZmleRUgmatU15wcs89Fb4B07QAxnAtteSCUy6Jq8ELAG40BdlSUTeSGEH4Obo49N3G4ccIKdtNSB66zvUwjMnqminDlND_JFXNmZRUTT3f_UWuhzH25RmWSmYkKd9vCqUPlI9DShFau41h4-LeUmInh-zaTkbYyQg7OWR_OWR3Rfr2OGC830BzEv62pADvj4BL3nVtdL0P6Q_Huea1mXb4eOCeQgf7_17ALq4up6zoq4M-pAy7k97F77ZWXEl7d7O0Qq3E8q6-sSv-Ey0huVY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1529501119</pqid></control><display><type>article</type><title>Serum total tryptase levels are increased in patients with active chronic urticaria</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Ferrer, M. ; Nuñez-Córdoba, J. M. ; Luquin, E. ; Grattan, C. E. ; De la Borbolla, J. M. ; Sanz, M. L. ; Schwartz, L. B.</creator><creatorcontrib>Ferrer, M. ; Nuñez-Córdoba, J. M. ; Luquin, E. ; Grattan, C. E. ; De la Borbolla, J. M. ; Sanz, M. L. ; Schwartz, L. B.</creatorcontrib><description>Summary
Background
We have demonstrated previously mast cell histamine release upon incubation with chronic urticaria (CU) sera, presumably by degranulation.
Objective
To explore total and mature tryptase in order to assess whether any increase in total tryptase levels is due in part to mast cell degranulation or to mast cell burden. We also wanted to explore differences between the autoimmune groups called idiopathic (serum unable to activate basophils), and to correlate total and mature tryptase levels with different urticaria features.
Methods
We measured total and mature tryptase serum levels in 81 CU patients, 16 atopic donors and 21 healthy control sera. We assessed autoimmunity by measuring the CD63 expression in normal basophil donors upon incubation with CU sera.
Results
We found significantly higher levels of total tryptase in the sera of CU patients (6.6 ±4.1 μg/L) than in sera from healthy non‐atopic subjects (4.4 ±2.8 μg/L) and from atopic subjects (4.5 ±1.7 μg/L). Mature tryptase levels were undetectable (<1 ng/mL). Total tryptase levels in the autoimmune urticaria group were significantly higher (9.8 ±5.4 μg/L) than the idiopathic urticaria group (4.4 ±2.2 μg/L). A significant difference in total tryptase was found between symptomatic patients (7.3 ±4.1 μg/L) compared with asymptomatic ones (5.7 ±4.1 μg/L) at the time of venesection. No difference was found in mature tryptase levels either.
Conclusion
Total elevated tryptase levels are not accompanied by an elevated mature tryptase levels, as might be expected if the serum levels reflected mast cell degranulation.
Cite this as: M. Ferrer, J. M. Nuñez‐Córdoba, E. Luquin, C. E. Grattan, J. M. De la Borbolla, M. L. Sanz, L. B. Schwartz, Clinical & Experimental Allergy, 2010 (40) 1760–1766.</description><identifier>ISSN: 0954-7894</identifier><identifier>EISSN: 1365-2222</identifier><identifier>DOI: 10.1111/j.1365-2222.2010.03582.x</identifier><identifier>PMID: 20718781</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adult ; Aged ; Allergic diseases ; Antigens, CD - analysis ; Antigens, CD - immunology ; Autoimmunity ; Basophils - immunology ; Biological and medical sciences ; Cell Degranulation ; Chronic Disease ; chronic urticaria ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Histamine ; Humans ; Immunopathology ; Mast cells ; Mast Cells - physiology ; Medical sciences ; Middle Aged ; Platelet Membrane Glycoproteins - analysis ; Platelet Membrane Glycoproteins - immunology ; Skin allergic diseases. Stinging insect allergies ; Tetraspanin 30 ; Tryptase ; Tryptases - blood ; Urticaria ; Urticaria - blood ; Urticaria - immunology ; Young Adult</subject><ispartof>Clinical and experimental allergy, 2010-12, Vol.40 (12), p.1760-1766</ispartof><rights>2010 Blackwell Publishing Ltd</rights><rights>2015 INIST-CNRS</rights><rights>2010 Blackwell Publishing Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4962-5315e82fa7c430b3c14cde9dfee99385853431350a6ec4ee3981e5f146d509403</citedby><cites>FETCH-LOGICAL-c4962-5315e82fa7c430b3c14cde9dfee99385853431350a6ec4ee3981e5f146d509403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2222.2010.03582.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2222.2010.03582.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23383699$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20718781$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ferrer, M.</creatorcontrib><creatorcontrib>Nuñez-Córdoba, J. M.</creatorcontrib><creatorcontrib>Luquin, E.</creatorcontrib><creatorcontrib>Grattan, C. E.</creatorcontrib><creatorcontrib>De la Borbolla, J. M.</creatorcontrib><creatorcontrib>Sanz, M. L.</creatorcontrib><creatorcontrib>Schwartz, L. B.</creatorcontrib><title>Serum total tryptase levels are increased in patients with active chronic urticaria</title><title>Clinical and experimental allergy</title><addtitle>Clin Exp Allergy</addtitle><description>Summary
Background
We have demonstrated previously mast cell histamine release upon incubation with chronic urticaria (CU) sera, presumably by degranulation.
Objective
To explore total and mature tryptase in order to assess whether any increase in total tryptase levels is due in part to mast cell degranulation or to mast cell burden. We also wanted to explore differences between the autoimmune groups called idiopathic (serum unable to activate basophils), and to correlate total and mature tryptase levels with different urticaria features.
Methods
We measured total and mature tryptase serum levels in 81 CU patients, 16 atopic donors and 21 healthy control sera. We assessed autoimmunity by measuring the CD63 expression in normal basophil donors upon incubation with CU sera.
Results
We found significantly higher levels of total tryptase in the sera of CU patients (6.6 ±4.1 μg/L) than in sera from healthy non‐atopic subjects (4.4 ±2.8 μg/L) and from atopic subjects (4.5 ±1.7 μg/L). Mature tryptase levels were undetectable (<1 ng/mL). Total tryptase levels in the autoimmune urticaria group were significantly higher (9.8 ±5.4 μg/L) than the idiopathic urticaria group (4.4 ±2.2 μg/L). A significant difference in total tryptase was found between symptomatic patients (7.3 ±4.1 μg/L) compared with asymptomatic ones (5.7 ±4.1 μg/L) at the time of venesection. No difference was found in mature tryptase levels either.
Conclusion
Total elevated tryptase levels are not accompanied by an elevated mature tryptase levels, as might be expected if the serum levels reflected mast cell degranulation.
Cite this as: M. Ferrer, J. M. Nuñez‐Córdoba, E. Luquin, C. E. Grattan, J. M. De la Borbolla, M. L. Sanz, L. B. Schwartz, Clinical & Experimental Allergy, 2010 (40) 1760–1766.</description><subject>Adult</subject><subject>Aged</subject><subject>Allergic diseases</subject><subject>Antigens, CD - analysis</subject><subject>Antigens, CD - immunology</subject><subject>Autoimmunity</subject><subject>Basophils - immunology</subject><subject>Biological and medical sciences</subject><subject>Cell Degranulation</subject><subject>Chronic Disease</subject><subject>chronic urticaria</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Histamine</subject><subject>Humans</subject><subject>Immunopathology</subject><subject>Mast cells</subject><subject>Mast Cells - physiology</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Platelet Membrane Glycoproteins - analysis</subject><subject>Platelet Membrane Glycoproteins - immunology</subject><subject>Skin allergic diseases. Stinging insect allergies</subject><subject>Tetraspanin 30</subject><subject>Tryptase</subject><subject>Tryptases - blood</subject><subject>Urticaria</subject><subject>Urticaria - blood</subject><subject>Urticaria - immunology</subject><subject>Young Adult</subject><issn>0954-7894</issn><issn>1365-2222</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1vEzEQhi0EoqHwF5AlhHra4M-1feBQRSVBqsqhoB4t1zurOmx2g-1tk3-Pl4QgcYG5eDR-3pmxX4QwJXNa4sN6TnktK1ZizkipEi41m--eodnp4jmaESNFpbQRZ-hVSmtCCmb0S3TGiKJaaTpDt7cQxw3OQ3YdznG_zS4B7uARuoRdBBx6H6HUmpLhrcsB-pzwU8gP2PkcHgH7hzj0weMx5uBdDO41etG6LsGb43mOvn26-rpYVddflp8Xl9eVF6ZmleRUgmatU15wcs89Fb4B07QAxnAtteSCUy6Jq8ELAG40BdlSUTeSGEH4Obo49N3G4ccIKdtNSB66zvUwjMnqminDlND_JFXNmZRUTT3f_UWuhzH25RmWSmYkKd9vCqUPlI9DShFau41h4-LeUmInh-zaTkbYyQg7OWR_OWR3Rfr2OGC830BzEv62pADvj4BL3nVtdL0P6Q_Huea1mXb4eOCeQgf7_17ALq4up6zoq4M-pAy7k97F77ZWXEl7d7O0Qq3E8q6-sSv-Ey0huVY</recordid><startdate>201012</startdate><enddate>201012</enddate><creator>Ferrer, M.</creator><creator>Nuñez-Córdoba, J. M.</creator><creator>Luquin, E.</creator><creator>Grattan, C. E.</creator><creator>De la Borbolla, J. M.</creator><creator>Sanz, M. L.</creator><creator>Schwartz, L. B.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>201012</creationdate><title>Serum total tryptase levels are increased in patients with active chronic urticaria</title><author>Ferrer, M. ; Nuñez-Córdoba, J. M. ; Luquin, E. ; Grattan, C. E. ; De la Borbolla, J. M. ; Sanz, M. L. ; Schwartz, L. B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4962-5315e82fa7c430b3c14cde9dfee99385853431350a6ec4ee3981e5f146d509403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Allergic diseases</topic><topic>Antigens, CD - analysis</topic><topic>Antigens, CD - immunology</topic><topic>Autoimmunity</topic><topic>Basophils - immunology</topic><topic>Biological and medical sciences</topic><topic>Cell Degranulation</topic><topic>Chronic Disease</topic><topic>chronic urticaria</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Histamine</topic><topic>Humans</topic><topic>Immunopathology</topic><topic>Mast cells</topic><topic>Mast Cells - physiology</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Platelet Membrane Glycoproteins - analysis</topic><topic>Platelet Membrane Glycoproteins - immunology</topic><topic>Skin allergic diseases. Stinging insect allergies</topic><topic>Tetraspanin 30</topic><topic>Tryptase</topic><topic>Tryptases - blood</topic><topic>Urticaria</topic><topic>Urticaria - blood</topic><topic>Urticaria - immunology</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ferrer, M.</creatorcontrib><creatorcontrib>Nuñez-Córdoba, J. M.</creatorcontrib><creatorcontrib>Luquin, E.</creatorcontrib><creatorcontrib>Grattan, C. E.</creatorcontrib><creatorcontrib>De la Borbolla, J. M.</creatorcontrib><creatorcontrib>Sanz, M. L.</creatorcontrib><creatorcontrib>Schwartz, L. B.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental allergy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ferrer, M.</au><au>Nuñez-Córdoba, J. M.</au><au>Luquin, E.</au><au>Grattan, C. E.</au><au>De la Borbolla, J. M.</au><au>Sanz, M. L.</au><au>Schwartz, L. B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serum total tryptase levels are increased in patients with active chronic urticaria</atitle><jtitle>Clinical and experimental allergy</jtitle><addtitle>Clin Exp Allergy</addtitle><date>2010-12</date><risdate>2010</risdate><volume>40</volume><issue>12</issue><spage>1760</spage><epage>1766</epage><pages>1760-1766</pages><issn>0954-7894</issn><eissn>1365-2222</eissn><abstract>Summary
Background
We have demonstrated previously mast cell histamine release upon incubation with chronic urticaria (CU) sera, presumably by degranulation.
Objective
To explore total and mature tryptase in order to assess whether any increase in total tryptase levels is due in part to mast cell degranulation or to mast cell burden. We also wanted to explore differences between the autoimmune groups called idiopathic (serum unable to activate basophils), and to correlate total and mature tryptase levels with different urticaria features.
Methods
We measured total and mature tryptase serum levels in 81 CU patients, 16 atopic donors and 21 healthy control sera. We assessed autoimmunity by measuring the CD63 expression in normal basophil donors upon incubation with CU sera.
Results
We found significantly higher levels of total tryptase in the sera of CU patients (6.6 ±4.1 μg/L) than in sera from healthy non‐atopic subjects (4.4 ±2.8 μg/L) and from atopic subjects (4.5 ±1.7 μg/L). Mature tryptase levels were undetectable (<1 ng/mL). Total tryptase levels in the autoimmune urticaria group were significantly higher (9.8 ±5.4 μg/L) than the idiopathic urticaria group (4.4 ±2.2 μg/L). A significant difference in total tryptase was found between symptomatic patients (7.3 ±4.1 μg/L) compared with asymptomatic ones (5.7 ±4.1 μg/L) at the time of venesection. No difference was found in mature tryptase levels either.
Conclusion
Total elevated tryptase levels are not accompanied by an elevated mature tryptase levels, as might be expected if the serum levels reflected mast cell degranulation.
Cite this as: M. Ferrer, J. M. Nuñez‐Córdoba, E. Luquin, C. E. Grattan, J. M. De la Borbolla, M. L. Sanz, L. B. Schwartz, Clinical & Experimental Allergy, 2010 (40) 1760–1766.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>20718781</pmid><doi>10.1111/j.1365-2222.2010.03582.x</doi><tpages>7</tpages></addata></record> |
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| ispartof | Clinical and experimental allergy, 2010-12, Vol.40 (12), p.1760-1766 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adult Aged Allergic diseases Antigens, CD - analysis Antigens, CD - immunology Autoimmunity Basophils - immunology Biological and medical sciences Cell Degranulation Chronic Disease chronic urticaria Fundamental and applied biological sciences. Psychology Fundamental immunology Histamine Humans Immunopathology Mast cells Mast Cells - physiology Medical sciences Middle Aged Platelet Membrane Glycoproteins - analysis Platelet Membrane Glycoproteins - immunology Skin allergic diseases. Stinging insect allergies Tetraspanin 30 Tryptase Tryptases - blood Urticaria Urticaria - blood Urticaria - immunology Young Adult |
| title | Serum total tryptase levels are increased in patients with active chronic urticaria |
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