Stimulatory Effect of LPS and Feedback Effect of PGE2 on IL‐27 production
Interleukin (IL)‐27 is a new member of the IL‐6/IL‐12 family, composed of two subunits, the Epstein-Barr virus-induced gene 3 (EBI3) and p28 chains (p28), and produced by activated monocytes and dendritic cells. IL‐27 plays an important role in the regulation of differentiation of naive T helper cel...
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| Veröffentlicht in: | Scandinavian journal of immunology 2010-12, Vol.72 (6), p.469-475 |
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| container_title | Scandinavian journal of immunology |
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| creator | Zhu, C.L Cao, Y.H Zhang, R Song, Y Liu, W.Y Pan, F Li, Y Zhu, Y Liu, F Wu, J.G |
| description | Interleukin (IL)‐27 is a new member of the IL‐6/IL‐12 family, composed of two subunits, the Epstein-Barr virus-induced gene 3 (EBI3) and p28 chains (p28), and produced by activated monocytes and dendritic cells. IL‐27 plays an important role in the regulation of differentiation of naive T helper cells and has diverse effects on innate immune cells. However, the pro‐inflammatory mechanisms of IL‐27 are still not well known. In this study, we investigated the effect of lipopolysaccharide (LPS) on the production of IL‐27. We found that LPS‐stimulated IL‐27 production was in a dose‐dependent and time‐dependent manner in THP‐1 cells. We have also shown that IL‐27 induced PGE2 production and COX‐2 gene expression at the level of mRNA as well as protein. Moreover, we found feed back effect of PGE2 on the production of IL‐27 in THP‐1 cells. The results suggest that PGE2 significantly inhibits LPS‐induced IL‐27 production, without affecting basal IL‐27 expression. Further experiment suggests that PGE2 and LPS regulate IL‐27 through NF‐κB pathway. Our findings may have wide implication for IL‐27 in inflammatory diseases. |
| doi_str_mv | 10.1111/j.1365-3083.2010.02460.x |
| format | Article |
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IL‐27 plays an important role in the regulation of differentiation of naive T helper cells and has diverse effects on innate immune cells. However, the pro‐inflammatory mechanisms of IL‐27 are still not well known. In this study, we investigated the effect of lipopolysaccharide (LPS) on the production of IL‐27. We found that LPS‐stimulated IL‐27 production was in a dose‐dependent and time‐dependent manner in THP‐1 cells. We have also shown that IL‐27 induced PGE2 production and COX‐2 gene expression at the level of mRNA as well as protein. Moreover, we found feed back effect of PGE2 on the production of IL‐27 in THP‐1 cells. The results suggest that PGE2 significantly inhibits LPS‐induced IL‐27 production, without affecting basal IL‐27 expression. Further experiment suggests that PGE2 and LPS regulate IL‐27 through NF‐κB pathway. Our findings may have wide implication for IL‐27 in inflammatory diseases.</description><identifier>ISSN: 0300-9475</identifier><identifier>EISSN: 1365-3083</identifier><identifier>DOI: 10.1111/j.1365-3083.2010.02460.x</identifier><identifier>PMID: 21044121</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Cell Line ; Cyclooxygenase 2 - metabolism ; Cyclooxygenase-2 ; Dendritic cells ; Differentiation ; Dinoprostone - metabolism ; Dose-Response Relationship, Immunologic ; Feedback ; Feedback, Physiological ; Gene expression ; Helper cells ; Humans ; Inflammatory diseases ; Interleukin 27 ; Interleukin 6 ; Interleukin-17 - immunology ; Interleukin-17 - metabolism ; Lipopolysaccharides ; Lipopolysaccharides - immunology ; Lymphocytes T ; Monocytes ; Monocytes - immunology ; NF- Kappa B protein ; Prostaglandin E2</subject><ispartof>Scandinavian journal of immunology, 2010-12, Vol.72 (6), p.469-475</ispartof><rights>2010 The Authors. Scandinavian Journal of Immunology © 2010 Blackwell Publishing Ltd</rights><rights>2010 The Authors. 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Our findings may have wide implication for IL‐27 in inflammatory diseases.</description><subject>Cell Line</subject><subject>Cyclooxygenase 2 - metabolism</subject><subject>Cyclooxygenase-2</subject><subject>Dendritic cells</subject><subject>Differentiation</subject><subject>Dinoprostone - metabolism</subject><subject>Dose-Response Relationship, Immunologic</subject><subject>Feedback</subject><subject>Feedback, Physiological</subject><subject>Gene expression</subject><subject>Helper cells</subject><subject>Humans</subject><subject>Inflammatory diseases</subject><subject>Interleukin 27</subject><subject>Interleukin 6</subject><subject>Interleukin-17 - immunology</subject><subject>Interleukin-17 - metabolism</subject><subject>Lipopolysaccharides</subject><subject>Lipopolysaccharides - immunology</subject><subject>Lymphocytes T</subject><subject>Monocytes</subject><subject>Monocytes - immunology</subject><subject>NF- Kappa B protein</subject><subject>Prostaglandin E2</subject><issn>0300-9475</issn><issn>1365-3083</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1OwzAQhS0EoqVwBfCOVcr4J3ayYIGqthQiUal0bbmOg1KSpuRHtDuOwBk5CQ4tFTu8seX3zYz9HkKYQJ-4dbPsEyZ8j0HA-hTcLVAuoL85Qt2DcIy6wAC8kEu_g86qaglAGJXsFHUoAc4JJV30OKvTvMl0XZRbPEwSa2pcJDiazrBexXhkbbzQ5vWPNB0PKS5WeBJ9fXxSiddlETemTovVOTpJdFbZi_3eQ_PR8Hlw70VP48ngLvKMGwoetzQ2VMQ--LEGw5gRwsbMuDdZxo0x7kOUcaAJJ9aY0BckINIYIoSUiQ1ZD13v-rrRb42tapWnlbFZple2aCoVCCpD4IL9S0pBuQQ_CBx5uSebRW5jtS7TXJdb9euUA253wHua2e1BJ6DaRNRStcar1njVJqJ-ElEbNXuYtCdXf7WrT3Sh9EuZVmo-cyQDEkIQkoB9A3eHhNc</recordid><startdate>201012</startdate><enddate>201012</enddate><creator>Zhu, C.L</creator><creator>Cao, Y.H</creator><creator>Zhang, R</creator><creator>Song, Y</creator><creator>Liu, W.Y</creator><creator>Pan, F</creator><creator>Li, Y</creator><creator>Zhu, Y</creator><creator>Liu, F</creator><creator>Wu, J.G</creator><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>201012</creationdate><title>Stimulatory Effect of LPS and Feedback Effect of PGE2 on IL‐27 production</title><author>Zhu, C.L ; Cao, Y.H ; Zhang, R ; Song, Y ; Liu, W.Y ; Pan, F ; Li, Y ; Zhu, Y ; Liu, F ; Wu, J.G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4410-4e2dc26d505da0c33c66ed3c104e34ccc01023402f41ecc9561817cc16677fe93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Cell Line</topic><topic>Cyclooxygenase 2 - metabolism</topic><topic>Cyclooxygenase-2</topic><topic>Dendritic cells</topic><topic>Differentiation</topic><topic>Dinoprostone - metabolism</topic><topic>Dose-Response Relationship, Immunologic</topic><topic>Feedback</topic><topic>Feedback, Physiological</topic><topic>Gene expression</topic><topic>Helper cells</topic><topic>Humans</topic><topic>Inflammatory diseases</topic><topic>Interleukin 27</topic><topic>Interleukin 6</topic><topic>Interleukin-17 - immunology</topic><topic>Interleukin-17 - metabolism</topic><topic>Lipopolysaccharides</topic><topic>Lipopolysaccharides - immunology</topic><topic>Lymphocytes T</topic><topic>Monocytes</topic><topic>Monocytes - immunology</topic><topic>NF- Kappa B protein</topic><topic>Prostaglandin E2</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, C.L</creatorcontrib><creatorcontrib>Cao, Y.H</creatorcontrib><creatorcontrib>Zhang, R</creatorcontrib><creatorcontrib>Song, Y</creatorcontrib><creatorcontrib>Liu, W.Y</creatorcontrib><creatorcontrib>Pan, F</creatorcontrib><creatorcontrib>Li, Y</creatorcontrib><creatorcontrib>Zhu, Y</creatorcontrib><creatorcontrib>Liu, F</creatorcontrib><creatorcontrib>Wu, J.G</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Scandinavian journal of immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, C.L</au><au>Cao, Y.H</au><au>Zhang, R</au><au>Song, Y</au><au>Liu, W.Y</au><au>Pan, F</au><au>Li, Y</au><au>Zhu, Y</au><au>Liu, F</au><au>Wu, J.G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stimulatory Effect of LPS and Feedback Effect of PGE2 on IL‐27 production</atitle><jtitle>Scandinavian journal of immunology</jtitle><addtitle>Scand J Immunol</addtitle><date>2010-12</date><risdate>2010</risdate><volume>72</volume><issue>6</issue><spage>469</spage><epage>475</epage><pages>469-475</pages><issn>0300-9475</issn><eissn>1365-3083</eissn><abstract>Interleukin (IL)‐27 is a new member of the IL‐6/IL‐12 family, composed of two subunits, the Epstein-Barr virus-induced gene 3 (EBI3) and p28 chains (p28), and produced by activated monocytes and dendritic cells. IL‐27 plays an important role in the regulation of differentiation of naive T helper cells and has diverse effects on innate immune cells. However, the pro‐inflammatory mechanisms of IL‐27 are still not well known. In this study, we investigated the effect of lipopolysaccharide (LPS) on the production of IL‐27. We found that LPS‐stimulated IL‐27 production was in a dose‐dependent and time‐dependent manner in THP‐1 cells. We have also shown that IL‐27 induced PGE2 production and COX‐2 gene expression at the level of mRNA as well as protein. Moreover, we found feed back effect of PGE2 on the production of IL‐27 in THP‐1 cells. The results suggest that PGE2 significantly inhibits LPS‐induced IL‐27 production, without affecting basal IL‐27 expression. Further experiment suggests that PGE2 and LPS regulate IL‐27 through NF‐κB pathway. 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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE; IngentaConnect Free/Open Access Journals; Wiley Online Library Free Content; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Cell Line Cyclooxygenase 2 - metabolism Cyclooxygenase-2 Dendritic cells Differentiation Dinoprostone - metabolism Dose-Response Relationship, Immunologic Feedback Feedback, Physiological Gene expression Helper cells Humans Inflammatory diseases Interleukin 27 Interleukin 6 Interleukin-17 - immunology Interleukin-17 - metabolism Lipopolysaccharides Lipopolysaccharides - immunology Lymphocytes T Monocytes Monocytes - immunology NF- Kappa B protein Prostaglandin E2 |
| title | Stimulatory Effect of LPS and Feedback Effect of PGE2 on IL‐27 production |
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