Prebiotic oligosaccharides: In vitro evidence for gastrointestinal epithelial transfer and immunomodulatory properties
Eiwegger T, Stahl B, Haidl P, Schmitt J, Boehm G, Dehlink E, Urbanek R, Szépfalusi Z. Prebiotic oligosaccharides: In vitro evidence for gastrointestinal epithelial transfer and immunomodulatory properties. Pediatr Allergy Immunol 2010: 21: 1179–1188. © 2010 John Wiley & Sons A/S Prebiotic oligos...
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| description | Eiwegger T, Stahl B, Haidl P, Schmitt J, Boehm G, Dehlink E, Urbanek R, Szépfalusi Z. Prebiotic oligosaccharides: In vitro evidence for gastrointestinal epithelial transfer and immunomodulatory properties.
Pediatr Allergy Immunol 2010: 21: 1179–1188.
© 2010 John Wiley & Sons A/S
Prebiotic oligosaccharides are present in breast milk and evidence is pointing toward immunomodulatory properties of the acidic fraction. Recently, prebiotic supplements of infant formula [short‐chain galacto (scGOS)‐, long‐chain fructo (lcFOS)‐oligosaccharides] showed preventive effects on atopic disease development.
We aimed to define the direct immunologic effects of these oligosaccharides and of human (aHMOS) and cows’ milk (aCMOS) acidic oligosaccharides and to investigate the systemic uptake of prebiotic supplements of infant formula and a specific pectin‐derived acidic oligosaccharide hydrolysate (pAOS) in vitro.
After assurance of LPS‐free conditions (limulus assay, toll like receptor‐2, ‐4 transfected human embryonic kidney‐cells), in vitro‐transfer through a CaCo‐2 cell monolayer was measured using high‐pH anion exchange chromatography with pulsed amperometric detection. Direct effects on proliferation, cytokine‐induction of cord blood mononuclear cells and modulation of allergen‐specific CD4+ T‐cell cytokine profiles from allergic and non‐allergic individuals were investigated.
Transfer of scGOS/lcFOS and pAOS in‐vitro was detected with a rate of transfer of 4–14%, depending on the molecular size and structure. AHMOS induced IFN‐γ and IL‐10 but not the Th‐2 cytokine IL‐13 at physiologic concentrations (10–100 μg/ml) in cord blood, whereas aCMOS did not induce any of these cytokines. AHMOS significantly suppressed Th‐2 type cytokine‐production by Ara h1‐specific CD4+ T cells (CFSElow CD3+CD4+cells) from peanut allergic patients.
In conclusion, human milk‐derived acidic oligosaccharides may modulate postnatal allergen‐specific immune responses by the suppression of Th‐2‐type responses in atopy‐prone individuals. Moreover, there is in vitro evidence for epithelial transport of prebiotic oligosaccharides. |
| doi_str_mv | 10.1111/j.1399-3038.2010.01062.x |
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Pediatr Allergy Immunol 2010: 21: 1179–1188.
© 2010 John Wiley & Sons A/S
Prebiotic oligosaccharides are present in breast milk and evidence is pointing toward immunomodulatory properties of the acidic fraction. Recently, prebiotic supplements of infant formula [short‐chain galacto (scGOS)‐, long‐chain fructo (lcFOS)‐oligosaccharides] showed preventive effects on atopic disease development.
We aimed to define the direct immunologic effects of these oligosaccharides and of human (aHMOS) and cows’ milk (aCMOS) acidic oligosaccharides and to investigate the systemic uptake of prebiotic supplements of infant formula and a specific pectin‐derived acidic oligosaccharide hydrolysate (pAOS) in vitro.
After assurance of LPS‐free conditions (limulus assay, toll like receptor‐2, ‐4 transfected human embryonic kidney‐cells), in vitro‐transfer through a CaCo‐2 cell monolayer was measured using high‐pH anion exchange chromatography with pulsed amperometric detection. Direct effects on proliferation, cytokine‐induction of cord blood mononuclear cells and modulation of allergen‐specific CD4+ T‐cell cytokine profiles from allergic and non‐allergic individuals were investigated.
Transfer of scGOS/lcFOS and pAOS in‐vitro was detected with a rate of transfer of 4–14%, depending on the molecular size and structure. AHMOS induced IFN‐γ and IL‐10 but not the Th‐2 cytokine IL‐13 at physiologic concentrations (10–100 μg/ml) in cord blood, whereas aCMOS did not induce any of these cytokines. AHMOS significantly suppressed Th‐2 type cytokine‐production by Ara h1‐specific CD4+ T cells (CFSElow CD3+CD4+cells) from peanut allergic patients.
In conclusion, human milk‐derived acidic oligosaccharides may modulate postnatal allergen‐specific immune responses by the suppression of Th‐2‐type responses in atopy‐prone individuals. Moreover, there is in vitro evidence for epithelial transport of prebiotic oligosaccharides.</description><identifier>ISSN: 0905-6157</identifier><identifier>EISSN: 1399-3038</identifier><identifier>DOI: 10.1111/j.1399-3038.2010.01062.x</identifier><identifier>PMID: 20444147</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Allergic diseases ; Animals ; Anions ; Antigens, Plant - immunology ; Arachis hypogaea ; Atopy ; Biological and medical sciences ; Biological Transport ; Breast milk ; Caco-2 Cells ; Cattle ; CD4 antigen ; Cell proliferation ; Chromatography ; Cord blood ; Cow's milk ; Dietary Fiber - metabolism ; Digestive allergic diseases ; Embryos ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; gamma -Interferon ; General aspects ; Glycoproteins - immunology ; human milk ; Humans ; Hydrolysates ; Hypersensitivity ; immunmodulation ; Immunomodulation ; Immunopathology ; Immunosuppression ; Infant Formula - administration & dosage ; Infant Formula - chemistry ; Infant formulas ; Interleukin 10 ; Interleukin 13 ; Intestinal Mucosa - drug effects ; Intestinal Mucosa - metabolism ; Leukocytes (mononuclear) ; Limulus ; Lymphocytes T ; Medical sciences ; Milk - metabolism ; Milk Hypersensitivity - diet therapy ; Milk Hypersensitivity - immunology ; Milk Hypersensitivity - microbiology ; Milk, Human - metabolism ; Nuts ; oligosaccharides ; Oligosaccharides - chemistry ; Oligosaccharides - metabolism ; peanut allergy ; Plant Proteins - immunology ; Prebiotics ; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis ; T-cell ; Th2 Cells - immunology</subject><ispartof>Pediatric allergy and immunology, 2010-12, Vol.21 (8), p.1179-1188</ispartof><rights>2010 John Wiley & Sons A/S</rights><rights>2015 INIST-CNRS</rights><rights>2010 John Wiley & Sons A/S.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5342-77fecd5743b7b238d10a3ba7ff8c5ba7927a3b04dad58484aa3c00636ec8283a3</citedby><cites>FETCH-LOGICAL-c5342-77fecd5743b7b238d10a3ba7ff8c5ba7927a3b04dad58484aa3c00636ec8283a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1399-3038.2010.01062.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1399-3038.2010.01062.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23432096$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20444147$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eiwegger, Thomas</creatorcontrib><creatorcontrib>Stahl, Bernd</creatorcontrib><creatorcontrib>Haidl, Paul</creatorcontrib><creatorcontrib>Schmitt, Joachim</creatorcontrib><creatorcontrib>Boehm, Günther</creatorcontrib><creatorcontrib>Dehlink, Eleonora</creatorcontrib><creatorcontrib>Urbanek, Radvan</creatorcontrib><creatorcontrib>Szépfalusi, Zsolt</creatorcontrib><title>Prebiotic oligosaccharides: In vitro evidence for gastrointestinal epithelial transfer and immunomodulatory properties</title><title>Pediatric allergy and immunology</title><addtitle>Pediatr Allergy Immunol</addtitle><description>Eiwegger T, Stahl B, Haidl P, Schmitt J, Boehm G, Dehlink E, Urbanek R, Szépfalusi Z. Prebiotic oligosaccharides: In vitro evidence for gastrointestinal epithelial transfer and immunomodulatory properties.
Pediatr Allergy Immunol 2010: 21: 1179–1188.
© 2010 John Wiley & Sons A/S
Prebiotic oligosaccharides are present in breast milk and evidence is pointing toward immunomodulatory properties of the acidic fraction. Recently, prebiotic supplements of infant formula [short‐chain galacto (scGOS)‐, long‐chain fructo (lcFOS)‐oligosaccharides] showed preventive effects on atopic disease development.
We aimed to define the direct immunologic effects of these oligosaccharides and of human (aHMOS) and cows’ milk (aCMOS) acidic oligosaccharides and to investigate the systemic uptake of prebiotic supplements of infant formula and a specific pectin‐derived acidic oligosaccharide hydrolysate (pAOS) in vitro.
After assurance of LPS‐free conditions (limulus assay, toll like receptor‐2, ‐4 transfected human embryonic kidney‐cells), in vitro‐transfer through a CaCo‐2 cell monolayer was measured using high‐pH anion exchange chromatography with pulsed amperometric detection. Direct effects on proliferation, cytokine‐induction of cord blood mononuclear cells and modulation of allergen‐specific CD4+ T‐cell cytokine profiles from allergic and non‐allergic individuals were investigated.
Transfer of scGOS/lcFOS and pAOS in‐vitro was detected with a rate of transfer of 4–14%, depending on the molecular size and structure. AHMOS induced IFN‐γ and IL‐10 but not the Th‐2 cytokine IL‐13 at physiologic concentrations (10–100 μg/ml) in cord blood, whereas aCMOS did not induce any of these cytokines. AHMOS significantly suppressed Th‐2 type cytokine‐production by Ara h1‐specific CD4+ T cells (CFSElow CD3+CD4+cells) from peanut allergic patients.
In conclusion, human milk‐derived acidic oligosaccharides may modulate postnatal allergen‐specific immune responses by the suppression of Th‐2‐type responses in atopy‐prone individuals. Moreover, there is in vitro evidence for epithelial transport of prebiotic oligosaccharides.</description><subject>Allergic diseases</subject><subject>Animals</subject><subject>Anions</subject><subject>Antigens, Plant - immunology</subject><subject>Arachis hypogaea</subject><subject>Atopy</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Breast milk</subject><subject>Caco-2 Cells</subject><subject>Cattle</subject><subject>CD4 antigen</subject><subject>Cell proliferation</subject><subject>Chromatography</subject><subject>Cord blood</subject><subject>Cow's milk</subject><subject>Dietary Fiber - metabolism</subject><subject>Digestive allergic diseases</subject><subject>Embryos</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>gamma -Interferon</subject><subject>General aspects</subject><subject>Glycoproteins - immunology</subject><subject>human milk</subject><subject>Humans</subject><subject>Hydrolysates</subject><subject>Hypersensitivity</subject><subject>immunmodulation</subject><subject>Immunomodulation</subject><subject>Immunopathology</subject><subject>Immunosuppression</subject><subject>Infant Formula - administration & dosage</subject><subject>Infant Formula - chemistry</subject><subject>Infant formulas</subject><subject>Interleukin 10</subject><subject>Interleukin 13</subject><subject>Intestinal Mucosa - drug effects</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Leukocytes (mononuclear)</subject><subject>Limulus</subject><subject>Lymphocytes T</subject><subject>Medical sciences</subject><subject>Milk - metabolism</subject><subject>Milk Hypersensitivity - diet therapy</subject><subject>Milk Hypersensitivity - immunology</subject><subject>Milk Hypersensitivity - microbiology</subject><subject>Milk, Human - metabolism</subject><subject>Nuts</subject><subject>oligosaccharides</subject><subject>Oligosaccharides - chemistry</subject><subject>Oligosaccharides - metabolism</subject><subject>peanut allergy</subject><subject>Plant Proteins - immunology</subject><subject>Prebiotics</subject><subject>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</subject><subject>T-cell</subject><subject>Th2 Cells - immunology</subject><issn>0905-6157</issn><issn>1399-3038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcGO0zAQhi0EYsvCKyBfEKcUO3ZiB4nDaoGl0gJ7KOrRmjiTXZckLnZa2rfHoaUcwZI14_H3e6z5CaGczXlab9ZzLqoqE0zoec5SNe0yn-8fkdn54jGZsYoVWckLdUGexbhmjCtR8qfkImdSSi7VjOzuAtbOj85S37l7H8HaBwiuwfiWLga6c2PwFHepMFikrQ_0HmKquWHEOLoBOoobNz5g51I6Bhhii4HC0FDX99vB977ZdjD6cKCb4DcYRofxOXnSQhfxxSlekm8fPyyvP2W3X28W11e3mS2EzDOlWrRNoaSoVZ0L3XAGogbVttoWKVa5SmcmG2gKLbUEEJaxUpRoda4FiEvy-vhuav1jmz5sehctdh0M6LfR6DJXuuKS_ZNUWk1DK3Ui9ZG0wccYsDWb4HoIB8OZmewxazO5YCYXzGSP-W2P2Sfpy1OTbd1jcxb-8SMBr04ARAtdm8ZpXfzLCSlyVpWJe3fkfroOD__9AXN3tZiypM-OehdH3J_1EL6bUglVmNWXG_NerD4vV1ybpfgFOvq9JQ</recordid><startdate>201012</startdate><enddate>201012</enddate><creator>Eiwegger, Thomas</creator><creator>Stahl, Bernd</creator><creator>Haidl, Paul</creator><creator>Schmitt, Joachim</creator><creator>Boehm, Günther</creator><creator>Dehlink, Eleonora</creator><creator>Urbanek, Radvan</creator><creator>Szépfalusi, Zsolt</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>201012</creationdate><title>Prebiotic oligosaccharides: In vitro evidence for gastrointestinal epithelial transfer and immunomodulatory properties</title><author>Eiwegger, Thomas ; Stahl, Bernd ; Haidl, Paul ; Schmitt, Joachim ; Boehm, Günther ; Dehlink, Eleonora ; Urbanek, Radvan ; Szépfalusi, Zsolt</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5342-77fecd5743b7b238d10a3ba7ff8c5ba7927a3b04dad58484aa3c00636ec8283a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Allergic diseases</topic><topic>Animals</topic><topic>Anions</topic><topic>Antigens, Plant - immunology</topic><topic>Arachis hypogaea</topic><topic>Atopy</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>Breast milk</topic><topic>Caco-2 Cells</topic><topic>Cattle</topic><topic>CD4 antigen</topic><topic>Cell proliferation</topic><topic>Chromatography</topic><topic>Cord blood</topic><topic>Cow's milk</topic><topic>Dietary Fiber - metabolism</topic><topic>Digestive allergic diseases</topic><topic>Embryos</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>gamma -Interferon</topic><topic>General aspects</topic><topic>Glycoproteins - immunology</topic><topic>human milk</topic><topic>Humans</topic><topic>Hydrolysates</topic><topic>Hypersensitivity</topic><topic>immunmodulation</topic><topic>Immunomodulation</topic><topic>Immunopathology</topic><topic>Immunosuppression</topic><topic>Infant Formula - administration & dosage</topic><topic>Infant Formula - chemistry</topic><topic>Infant formulas</topic><topic>Interleukin 10</topic><topic>Interleukin 13</topic><topic>Intestinal Mucosa - drug effects</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Leukocytes (mononuclear)</topic><topic>Limulus</topic><topic>Lymphocytes T</topic><topic>Medical sciences</topic><topic>Milk - metabolism</topic><topic>Milk Hypersensitivity - diet therapy</topic><topic>Milk Hypersensitivity - immunology</topic><topic>Milk Hypersensitivity - microbiology</topic><topic>Milk, Human - metabolism</topic><topic>Nuts</topic><topic>oligosaccharides</topic><topic>Oligosaccharides - chemistry</topic><topic>Oligosaccharides - metabolism</topic><topic>peanut allergy</topic><topic>Plant Proteins - immunology</topic><topic>Prebiotics</topic><topic>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</topic><topic>T-cell</topic><topic>Th2 Cells - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eiwegger, Thomas</creatorcontrib><creatorcontrib>Stahl, Bernd</creatorcontrib><creatorcontrib>Haidl, Paul</creatorcontrib><creatorcontrib>Schmitt, Joachim</creatorcontrib><creatorcontrib>Boehm, Günther</creatorcontrib><creatorcontrib>Dehlink, Eleonora</creatorcontrib><creatorcontrib>Urbanek, Radvan</creatorcontrib><creatorcontrib>Szépfalusi, Zsolt</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Pediatric allergy and immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eiwegger, Thomas</au><au>Stahl, Bernd</au><au>Haidl, Paul</au><au>Schmitt, Joachim</au><au>Boehm, Günther</au><au>Dehlink, Eleonora</au><au>Urbanek, Radvan</au><au>Szépfalusi, Zsolt</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prebiotic oligosaccharides: In vitro evidence for gastrointestinal epithelial transfer and immunomodulatory properties</atitle><jtitle>Pediatric allergy and immunology</jtitle><addtitle>Pediatr Allergy Immunol</addtitle><date>2010-12</date><risdate>2010</risdate><volume>21</volume><issue>8</issue><spage>1179</spage><epage>1188</epage><pages>1179-1188</pages><issn>0905-6157</issn><eissn>1399-3038</eissn><abstract>Eiwegger T, Stahl B, Haidl P, Schmitt J, Boehm G, Dehlink E, Urbanek R, Szépfalusi Z. Prebiotic oligosaccharides: In vitro evidence for gastrointestinal epithelial transfer and immunomodulatory properties.
Pediatr Allergy Immunol 2010: 21: 1179–1188.
© 2010 John Wiley & Sons A/S
Prebiotic oligosaccharides are present in breast milk and evidence is pointing toward immunomodulatory properties of the acidic fraction. Recently, prebiotic supplements of infant formula [short‐chain galacto (scGOS)‐, long‐chain fructo (lcFOS)‐oligosaccharides] showed preventive effects on atopic disease development.
We aimed to define the direct immunologic effects of these oligosaccharides and of human (aHMOS) and cows’ milk (aCMOS) acidic oligosaccharides and to investigate the systemic uptake of prebiotic supplements of infant formula and a specific pectin‐derived acidic oligosaccharide hydrolysate (pAOS) in vitro.
After assurance of LPS‐free conditions (limulus assay, toll like receptor‐2, ‐4 transfected human embryonic kidney‐cells), in vitro‐transfer through a CaCo‐2 cell monolayer was measured using high‐pH anion exchange chromatography with pulsed amperometric detection. Direct effects on proliferation, cytokine‐induction of cord blood mononuclear cells and modulation of allergen‐specific CD4+ T‐cell cytokine profiles from allergic and non‐allergic individuals were investigated.
Transfer of scGOS/lcFOS and pAOS in‐vitro was detected with a rate of transfer of 4–14%, depending on the molecular size and structure. AHMOS induced IFN‐γ and IL‐10 but not the Th‐2 cytokine IL‐13 at physiologic concentrations (10–100 μg/ml) in cord blood, whereas aCMOS did not induce any of these cytokines. AHMOS significantly suppressed Th‐2 type cytokine‐production by Ara h1‐specific CD4+ T cells (CFSElow CD3+CD4+cells) from peanut allergic patients.
In conclusion, human milk‐derived acidic oligosaccharides may modulate postnatal allergen‐specific immune responses by the suppression of Th‐2‐type responses in atopy‐prone individuals. Moreover, there is in vitro evidence for epithelial transport of prebiotic oligosaccharides.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>20444147</pmid><doi>10.1111/j.1399-3038.2010.01062.x</doi><tpages>10</tpages></addata></record> |
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| subjects | Allergic diseases Animals Anions Antigens, Plant - immunology Arachis hypogaea Atopy Biological and medical sciences Biological Transport Breast milk Caco-2 Cells Cattle CD4 antigen Cell proliferation Chromatography Cord blood Cow's milk Dietary Fiber - metabolism Digestive allergic diseases Embryos Fundamental and applied biological sciences. Psychology Fundamental immunology gamma -Interferon General aspects Glycoproteins - immunology human milk Humans Hydrolysates Hypersensitivity immunmodulation Immunomodulation Immunopathology Immunosuppression Infant Formula - administration & dosage Infant Formula - chemistry Infant formulas Interleukin 10 Interleukin 13 Intestinal Mucosa - drug effects Intestinal Mucosa - metabolism Leukocytes (mononuclear) Limulus Lymphocytes T Medical sciences Milk - metabolism Milk Hypersensitivity - diet therapy Milk Hypersensitivity - immunology Milk Hypersensitivity - microbiology Milk, Human - metabolism Nuts oligosaccharides Oligosaccharides - chemistry Oligosaccharides - metabolism peanut allergy Plant Proteins - immunology Prebiotics Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis T-cell Th2 Cells - immunology |
| title | Prebiotic oligosaccharides: In vitro evidence for gastrointestinal epithelial transfer and immunomodulatory properties |
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