Human breast tumor cells express IL-10 and IL-12p40 transcripts and proteins, but do not produce IL-12p70
IL-10 transcripts were expressed in 14/15 primary breast adenocarcinomas and in 5/8 established breast tumor lines. Immunohistochemistry and immunoprecipitation from lysates and supernatants revealed that established breast tumor lines produced IL-10 protein. Immunohistochemistry revealed that IL-10...
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| Veröffentlicht in: | Cellular immunology 2011, Vol.266 (2), p.143-153 |
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| creator | Heckel, Mark C. Wolfson, Alexey Slachta, Christopher A. Schwarting, Roland Salgame, Padmini Katsetos, Christos D. Platsoucas, Chris D. |
| description | IL-10 transcripts were expressed in 14/15 primary breast adenocarcinomas and in 5/8 established breast tumor lines. Immunohistochemistry and immunoprecipitation from lysates and supernatants revealed that established breast tumor lines produced IL-10 protein. Immunohistochemistry revealed that IL-10 is localized to tumor cells of primary breast adenocarcinomas and to occasional infiltrating MNC. Established breast tumor cell lines expressed IL-12p40 transcripts (6/8) and protein (4/7) and IL-12p35 transcripts (6/7). Using two sandwich ELISAs, specific, respectively, for IL-12p40 and IL-12p70 proteins, we demonstrated that established breast tumor cell lines produce IL-12p40 monomer/homodimer, but not IL-12p70. Positive staining for IL-12p70 in primary breast adenocarcinomas was found only in MNC infiltrating the tumor while tumor cells were negative. IL-12p40 homodimer/monomer inhibit as antagonists IL-12 or IL-23, although they may also act as agonists and positive regulators. Also, primary breast adenocarcinomas (15/15) and established breast tumor cell lines (6/8) expressed TGF-β1 transcripts. IL-10, IL-12p40 and TGF-β1 may inhibit substantially the anti-tumor immune response. |
| doi_str_mv | 10.1016/j.cellimm.2010.09.010 |
| format | Article |
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Immunohistochemistry and immunoprecipitation from lysates and supernatants revealed that established breast tumor lines produced IL-10 protein. Immunohistochemistry revealed that IL-10 is localized to tumor cells of primary breast adenocarcinomas and to occasional infiltrating MNC. Established breast tumor cell lines expressed IL-12p40 transcripts (6/8) and protein (4/7) and IL-12p35 transcripts (6/7). Using two sandwich ELISAs, specific, respectively, for IL-12p40 and IL-12p70 proteins, we demonstrated that established breast tumor cell lines produce IL-12p40 monomer/homodimer, but not IL-12p70. Positive staining for IL-12p70 in primary breast adenocarcinomas was found only in MNC infiltrating the tumor while tumor cells were negative. IL-12p40 homodimer/monomer inhibit as antagonists IL-12 or IL-23, although they may also act as agonists and positive regulators. Also, primary breast adenocarcinomas (15/15) and established breast tumor cell lines (6/8) expressed TGF-β1 transcripts. IL-10, IL-12p40 and TGF-β1 may inhibit substantially the anti-tumor immune response.</description><identifier>ISSN: 0008-8749</identifier><identifier>EISSN: 1090-2163</identifier><identifier>DOI: 10.1016/j.cellimm.2010.09.010</identifier><identifier>PMID: 21055733</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Adenocarcinoma - immunology ; Adenocarcinoma - metabolism ; Adult ; Aged ; Breast carcinoma ; Breast Neoplasms - immunology ; Breast Neoplasms - metabolism ; Cell Line, Tumor ; Cytokines ; Female ; Humans ; IL-10 ; IL-12p40 ; Immunosuppression ; Interleukin-10 - analysis ; Interleukin-10 - biosynthesis ; Interleukin-12 Subunit p35 - analysis ; Interleukin-12 Subunit p35 - biosynthesis ; Interleukin-12 Subunit p40 - analysis ; Interleukin-12 Subunit p40 - biosynthesis ; Interleukin-23 - agonists ; Interleukin-23 - antagonists & inhibitors ; Interleukin-23 - biosynthesis ; Middle Aged ; Transforming Growth Factor beta1 - analysis ; Transforming Growth Factor beta1 - immunology ; Tumor-infiltrating lymphocytes</subject><ispartof>Cellular immunology, 2011, Vol.266 (2), p.143-153</ispartof><rights>2010 Elsevier Inc.</rights><rights>Copyright © 2010 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-b7e5e0fe74fd26541ea2ebeb5e479c8cff3d5241a84aa289811aac53dab135933</citedby><cites>FETCH-LOGICAL-c396t-b7e5e0fe74fd26541ea2ebeb5e479c8cff3d5241a84aa289811aac53dab135933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0008874910002546$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21055733$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Heckel, Mark C.</creatorcontrib><creatorcontrib>Wolfson, Alexey</creatorcontrib><creatorcontrib>Slachta, Christopher A.</creatorcontrib><creatorcontrib>Schwarting, Roland</creatorcontrib><creatorcontrib>Salgame, Padmini</creatorcontrib><creatorcontrib>Katsetos, Christos D.</creatorcontrib><creatorcontrib>Platsoucas, Chris D.</creatorcontrib><title>Human breast tumor cells express IL-10 and IL-12p40 transcripts and proteins, but do not produce IL-12p70</title><title>Cellular immunology</title><addtitle>Cell Immunol</addtitle><description>IL-10 transcripts were expressed in 14/15 primary breast adenocarcinomas and in 5/8 established breast tumor lines. Immunohistochemistry and immunoprecipitation from lysates and supernatants revealed that established breast tumor lines produced IL-10 protein. Immunohistochemistry revealed that IL-10 is localized to tumor cells of primary breast adenocarcinomas and to occasional infiltrating MNC. Established breast tumor cell lines expressed IL-12p40 transcripts (6/8) and protein (4/7) and IL-12p35 transcripts (6/7). Using two sandwich ELISAs, specific, respectively, for IL-12p40 and IL-12p70 proteins, we demonstrated that established breast tumor cell lines produce IL-12p40 monomer/homodimer, but not IL-12p70. Positive staining for IL-12p70 in primary breast adenocarcinomas was found only in MNC infiltrating the tumor while tumor cells were negative. IL-12p40 homodimer/monomer inhibit as antagonists IL-12 or IL-23, although they may also act as agonists and positive regulators. Also, primary breast adenocarcinomas (15/15) and established breast tumor cell lines (6/8) expressed TGF-β1 transcripts. IL-10, IL-12p40 and TGF-β1 may inhibit substantially the anti-tumor immune response.</description><subject>Adenocarcinoma - immunology</subject><subject>Adenocarcinoma - metabolism</subject><subject>Adult</subject><subject>Aged</subject><subject>Breast carcinoma</subject><subject>Breast Neoplasms - immunology</subject><subject>Breast Neoplasms - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Cytokines</subject><subject>Female</subject><subject>Humans</subject><subject>IL-10</subject><subject>IL-12p40</subject><subject>Immunosuppression</subject><subject>Interleukin-10 - analysis</subject><subject>Interleukin-10 - biosynthesis</subject><subject>Interleukin-12 Subunit p35 - analysis</subject><subject>Interleukin-12 Subunit p35 - biosynthesis</subject><subject>Interleukin-12 Subunit p40 - analysis</subject><subject>Interleukin-12 Subunit p40 - biosynthesis</subject><subject>Interleukin-23 - agonists</subject><subject>Interleukin-23 - antagonists & inhibitors</subject><subject>Interleukin-23 - biosynthesis</subject><subject>Middle Aged</subject><subject>Transforming Growth Factor beta1 - analysis</subject><subject>Transforming Growth Factor beta1 - immunology</subject><subject>Tumor-infiltrating lymphocytes</subject><issn>0008-8749</issn><issn>1090-2163</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EotvCTwD5xoUsM_5I7BNCFbSVVuICZ8txJpJXmw9sB8G_b9JduMLptcbPO2PPy9gbhD0C1h-O-0CnUxyGvYC1Bna_yjO2Q7BQCazlc7YDAFOZRtkrdp3zEQBRWXjJrgSC1o2UOxbvl8GPvE3kc-FlGabEt8aZ0685Uc784VAhcD92TycxK-Al-TGHFOeSny7mNBWKY37P26XwbuLjVLZitwS6uBp4xV70_pTp9UVv2Pcvn7_d3leHr3cPt58OVZC2LlXbkCboqVF9J2qtkLygllpNqrHBhL6XnRYKvVHeC2MNovdBy863KLWV8oa9O_ddH_BjoVzcEPP2JT_StGRnatEYVM1_kALRSgNmJfWZDGnKOVHv5hQHn347BLfF4Y7uEofb4nBg3Sqr7-1lwtIO1P11_dn_Cnw8A7Ru5Gek5HKINAbqYqJQXDfFf4x4BHSYnFs</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>Heckel, Mark C.</creator><creator>Wolfson, Alexey</creator><creator>Slachta, Christopher A.</creator><creator>Schwarting, Roland</creator><creator>Salgame, Padmini</creator><creator>Katsetos, Christos D.</creator><creator>Platsoucas, Chris D.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>2011</creationdate><title>Human breast tumor cells express IL-10 and IL-12p40 transcripts and proteins, but do not produce IL-12p70</title><author>Heckel, Mark C. ; Wolfson, Alexey ; Slachta, Christopher A. ; Schwarting, Roland ; Salgame, Padmini ; Katsetos, Christos D. ; Platsoucas, Chris D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-b7e5e0fe74fd26541ea2ebeb5e479c8cff3d5241a84aa289811aac53dab135933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adenocarcinoma - immunology</topic><topic>Adenocarcinoma - metabolism</topic><topic>Adult</topic><topic>Aged</topic><topic>Breast carcinoma</topic><topic>Breast Neoplasms - immunology</topic><topic>Breast Neoplasms - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Cytokines</topic><topic>Female</topic><topic>Humans</topic><topic>IL-10</topic><topic>IL-12p40</topic><topic>Immunosuppression</topic><topic>Interleukin-10 - analysis</topic><topic>Interleukin-10 - biosynthesis</topic><topic>Interleukin-12 Subunit p35 - analysis</topic><topic>Interleukin-12 Subunit p35 - biosynthesis</topic><topic>Interleukin-12 Subunit p40 - analysis</topic><topic>Interleukin-12 Subunit p40 - biosynthesis</topic><topic>Interleukin-23 - agonists</topic><topic>Interleukin-23 - antagonists & inhibitors</topic><topic>Interleukin-23 - biosynthesis</topic><topic>Middle Aged</topic><topic>Transforming Growth Factor beta1 - analysis</topic><topic>Transforming Growth Factor beta1 - immunology</topic><topic>Tumor-infiltrating lymphocytes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heckel, Mark C.</creatorcontrib><creatorcontrib>Wolfson, Alexey</creatorcontrib><creatorcontrib>Slachta, Christopher A.</creatorcontrib><creatorcontrib>Schwarting, Roland</creatorcontrib><creatorcontrib>Salgame, Padmini</creatorcontrib><creatorcontrib>Katsetos, Christos D.</creatorcontrib><creatorcontrib>Platsoucas, Chris D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Cellular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heckel, Mark C.</au><au>Wolfson, Alexey</au><au>Slachta, Christopher A.</au><au>Schwarting, Roland</au><au>Salgame, Padmini</au><au>Katsetos, Christos D.</au><au>Platsoucas, Chris D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human breast tumor cells express IL-10 and IL-12p40 transcripts and proteins, but do not produce IL-12p70</atitle><jtitle>Cellular immunology</jtitle><addtitle>Cell Immunol</addtitle><date>2011</date><risdate>2011</risdate><volume>266</volume><issue>2</issue><spage>143</spage><epage>153</epage><pages>143-153</pages><issn>0008-8749</issn><eissn>1090-2163</eissn><abstract>IL-10 transcripts were expressed in 14/15 primary breast adenocarcinomas and in 5/8 established breast tumor lines. Immunohistochemistry and immunoprecipitation from lysates and supernatants revealed that established breast tumor lines produced IL-10 protein. Immunohistochemistry revealed that IL-10 is localized to tumor cells of primary breast adenocarcinomas and to occasional infiltrating MNC. Established breast tumor cell lines expressed IL-12p40 transcripts (6/8) and protein (4/7) and IL-12p35 transcripts (6/7). Using two sandwich ELISAs, specific, respectively, for IL-12p40 and IL-12p70 proteins, we demonstrated that established breast tumor cell lines produce IL-12p40 monomer/homodimer, but not IL-12p70. Positive staining for IL-12p70 in primary breast adenocarcinomas was found only in MNC infiltrating the tumor while tumor cells were negative. IL-12p40 homodimer/monomer inhibit as antagonists IL-12 or IL-23, although they may also act as agonists and positive regulators. Also, primary breast adenocarcinomas (15/15) and established breast tumor cell lines (6/8) expressed TGF-β1 transcripts. IL-10, IL-12p40 and TGF-β1 may inhibit substantially the anti-tumor immune response.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>21055733</pmid><doi>10.1016/j.cellimm.2010.09.010</doi><tpages>11</tpages></addata></record> |
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| subjects | Adenocarcinoma - immunology Adenocarcinoma - metabolism Adult Aged Breast carcinoma Breast Neoplasms - immunology Breast Neoplasms - metabolism Cell Line, Tumor Cytokines Female Humans IL-10 IL-12p40 Immunosuppression Interleukin-10 - analysis Interleukin-10 - biosynthesis Interleukin-12 Subunit p35 - analysis Interleukin-12 Subunit p35 - biosynthesis Interleukin-12 Subunit p40 - analysis Interleukin-12 Subunit p40 - biosynthesis Interleukin-23 - agonists Interleukin-23 - antagonists & inhibitors Interleukin-23 - biosynthesis Middle Aged Transforming Growth Factor beta1 - analysis Transforming Growth Factor beta1 - immunology Tumor-infiltrating lymphocytes |
| title | Human breast tumor cells express IL-10 and IL-12p40 transcripts and proteins, but do not produce IL-12p70 |
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