Occurrence and molecular characterization of Babesia species in a canine hospital population in the Londrina Region, Parana State, Brazil
Canine babesiosis is a worldwide disease caused by the protozoan of Babesia genus. Babesia canis and B. gibsoni are both species that naturally infect dogs. The objective of this study was to evaluate the infection of Babesia species in dogs attended at the Londrina State University Veterinary Teach...
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| Veröffentlicht in: | Revista brasileira de parasitologia veterinaria 2008-09, Vol.17 Suppl 1, p.277-283 |
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| creator | Shigueru, Flavio Jojima Garcia, João Luís Vidotto, Marilda Carlos Balarin, Mara Regina S Fabretti, Andrei Kleiton Gasparini, Marcela R Coelho, Adriana Letícia M Vidotto, Odilon |
| description | Canine babesiosis is a worldwide disease caused by the protozoan of Babesia genus. Babesia canis and B. gibsoni are both species that naturally infect dogs. The objective of this study was to evaluate the infection of Babesia species in dogs attended at the Londrina State University Veterinary Teaching Hospital (HV-UEL). It was selected 282 dogs seen at the Londrina State University Veterinary Teaching Hospital (HV-UEL) between April of 2005 and May of 2006. They presented anemia (Packed Cell Volume |
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Babesia canis and B. gibsoni are both species that naturally infect dogs. The objective of this study was to evaluate the infection of Babesia species in dogs attended at the Londrina State University Veterinary Teaching Hospital (HV-UEL). It was selected 282 dogs seen at the Londrina State University Veterinary Teaching Hospital (HV-UEL) between April of 2005 and May of 2006. They presented anemia (Packed Cell Volume<25%), thrombocytopenia (Platelet count <150000/mm3), leukopenia (White blood cell count<5000/mm3) or a combination of two or three of these alterations at the moment of the consultation. The presence of Babesia sp was determined by the amplification of a specific fragment of DNA of the Babesia genus by PCR. Microscopic examination of Giemsa-stained blood smears detected 38 (13.5%) positive samples against 105 identified by PCR from 282 dogs. The positive samples were submitted to PCR-RFLP by Hinf I that allows distinguishing the species of B. canis vogeli and B. gibsoni. From 282 dogs, Babesia sp infection was identified in 105 (37.2%). From these 105 positive samples, the PCR-RFLP identified 66 (23.4%) samples with a profile compatible to B. canis vogeli and 39 (13, 8%) to B. gibsoni. As conclusions, the results obtained allow to affirm that the babesiose is an important differential for dogs that present anemia, leukopenia and thrombocytopenia and, B. canis vogeli is the subspecies that is present in the most of the cases of babesiose in the population of dogs studied and, that B. gibsoni is also present causing babesiosis in dogs of the Londrina region, Parana State, Brazil.</description><identifier>EISSN: 1984-2961</identifier><identifier>PMID: 20059862</identifier><language>por</language><publisher>Brazil</publisher><subject>Animals ; Babesia - genetics ; Babesiosis - diagnosis ; Babesiosis - epidemiology ; Babesiosis - veterinary ; Brazil ; Dog Diseases - diagnosis ; Dog Diseases - epidemiology ; Dog Diseases - parasitology ; Dogs ; Hospitals, Animal ; Molecular Diagnostic Techniques</subject><ispartof>Revista brasileira de parasitologia veterinaria, 2008-09, Vol.17 Suppl 1, p.277-283</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20059862$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shigueru, Flavio Jojima</creatorcontrib><creatorcontrib>Garcia, João Luís</creatorcontrib><creatorcontrib>Vidotto, Marilda Carlos</creatorcontrib><creatorcontrib>Balarin, Mara Regina S</creatorcontrib><creatorcontrib>Fabretti, Andrei Kleiton</creatorcontrib><creatorcontrib>Gasparini, Marcela R</creatorcontrib><creatorcontrib>Coelho, Adriana Letícia M</creatorcontrib><creatorcontrib>Vidotto, Odilon</creatorcontrib><title>Occurrence and molecular characterization of Babesia species in a canine hospital population in the Londrina Region, Parana State, Brazil</title><title>Revista brasileira de parasitologia veterinaria</title><addtitle>Rev Bras Parasitol Vet</addtitle><description>Canine babesiosis is a worldwide disease caused by the protozoan of Babesia genus. Babesia canis and B. gibsoni are both species that naturally infect dogs. The objective of this study was to evaluate the infection of Babesia species in dogs attended at the Londrina State University Veterinary Teaching Hospital (HV-UEL). It was selected 282 dogs seen at the Londrina State University Veterinary Teaching Hospital (HV-UEL) between April of 2005 and May of 2006. They presented anemia (Packed Cell Volume<25%), thrombocytopenia (Platelet count <150000/mm3), leukopenia (White blood cell count<5000/mm3) or a combination of two or three of these alterations at the moment of the consultation. The presence of Babesia sp was determined by the amplification of a specific fragment of DNA of the Babesia genus by PCR. Microscopic examination of Giemsa-stained blood smears detected 38 (13.5%) positive samples against 105 identified by PCR from 282 dogs. The positive samples were submitted to PCR-RFLP by Hinf I that allows distinguishing the species of B. canis vogeli and B. gibsoni. From 282 dogs, Babesia sp infection was identified in 105 (37.2%). From these 105 positive samples, the PCR-RFLP identified 66 (23.4%) samples with a profile compatible to B. canis vogeli and 39 (13, 8%) to B. gibsoni. As conclusions, the results obtained allow to affirm that the babesiose is an important differential for dogs that present anemia, leukopenia and thrombocytopenia and, B. canis vogeli is the subspecies that is present in the most of the cases of babesiose in the population of dogs studied and, that B. gibsoni is also present causing babesiosis in dogs of the Londrina region, Parana State, Brazil.</description><subject>Animals</subject><subject>Babesia - genetics</subject><subject>Babesiosis - diagnosis</subject><subject>Babesiosis - epidemiology</subject><subject>Babesiosis - veterinary</subject><subject>Brazil</subject><subject>Dog Diseases - diagnosis</subject><subject>Dog Diseases - epidemiology</subject><subject>Dog Diseases - parasitology</subject><subject>Dogs</subject><subject>Hospitals, Animal</subject><subject>Molecular Diagnostic Techniques</subject><issn>1984-2961</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kMtOwzAQRSMkREvhF5B3bBrJsZ00XtKKl1SpCLqPJvaYGiV2sJMF_QP-GgvKajRz75zFOcvmhaxFzmRVzLLLGD8orSil_CKbMUpLWVdsnn3vlJpCQKeQgNOk9x2qqYNA1AECqBGDPcJovSPekDW0GC2QOKCyGIl1BIgCZx2Sg4-DHaEjgx8S4Pcl5eMBydY7HawD8orv6bwkLwmd1rcRRlySdYCj7a6ycwNdxOvTXGT7h_v95inf7h6fN3fbfChLlouSCWNUXVVSF0IYsWKy5bosqeasFWjA1JoaljLeSoW6aCsui1aKFVe1lnyR3f5hh-A_J4xj09uosOvAoZ9ik6ywSiZpqXlzak5tj7oZgu0hfDX_8vgPtgFr1g</recordid><startdate>200809</startdate><enddate>200809</enddate><creator>Shigueru, Flavio Jojima</creator><creator>Garcia, João Luís</creator><creator>Vidotto, Marilda Carlos</creator><creator>Balarin, Mara Regina S</creator><creator>Fabretti, Andrei Kleiton</creator><creator>Gasparini, Marcela R</creator><creator>Coelho, Adriana Letícia M</creator><creator>Vidotto, Odilon</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200809</creationdate><title>Occurrence and molecular characterization of Babesia species in a canine hospital population in the Londrina Region, Parana State, Brazil</title><author>Shigueru, Flavio Jojima ; Garcia, João Luís ; Vidotto, Marilda Carlos ; Balarin, Mara Regina S ; Fabretti, Andrei Kleiton ; Gasparini, Marcela R ; Coelho, Adriana Letícia M ; Vidotto, Odilon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p552-4524ffc8669d144f4729b3d550d32b4efaf8d0f21443b9ced1b6391b9473c8d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>por</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Babesia - genetics</topic><topic>Babesiosis - diagnosis</topic><topic>Babesiosis - epidemiology</topic><topic>Babesiosis - veterinary</topic><topic>Brazil</topic><topic>Dog Diseases - diagnosis</topic><topic>Dog Diseases - epidemiology</topic><topic>Dog Diseases - parasitology</topic><topic>Dogs</topic><topic>Hospitals, Animal</topic><topic>Molecular Diagnostic Techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shigueru, Flavio Jojima</creatorcontrib><creatorcontrib>Garcia, João Luís</creatorcontrib><creatorcontrib>Vidotto, Marilda Carlos</creatorcontrib><creatorcontrib>Balarin, Mara Regina S</creatorcontrib><creatorcontrib>Fabretti, Andrei Kleiton</creatorcontrib><creatorcontrib>Gasparini, Marcela R</creatorcontrib><creatorcontrib>Coelho, Adriana Letícia M</creatorcontrib><creatorcontrib>Vidotto, Odilon</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Revista brasileira de parasitologia veterinaria</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shigueru, Flavio Jojima</au><au>Garcia, João Luís</au><au>Vidotto, Marilda Carlos</au><au>Balarin, Mara Regina S</au><au>Fabretti, Andrei Kleiton</au><au>Gasparini, Marcela R</au><au>Coelho, Adriana Letícia M</au><au>Vidotto, Odilon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Occurrence and molecular characterization of Babesia species in a canine hospital population in the Londrina Region, Parana State, Brazil</atitle><jtitle>Revista brasileira de parasitologia veterinaria</jtitle><addtitle>Rev Bras Parasitol Vet</addtitle><date>2008-09</date><risdate>2008</risdate><volume>17 Suppl 1</volume><spage>277</spage><epage>283</epage><pages>277-283</pages><eissn>1984-2961</eissn><abstract>Canine babesiosis is a worldwide disease caused by the protozoan of Babesia genus. Babesia canis and B. gibsoni are both species that naturally infect dogs. The objective of this study was to evaluate the infection of Babesia species in dogs attended at the Londrina State University Veterinary Teaching Hospital (HV-UEL). It was selected 282 dogs seen at the Londrina State University Veterinary Teaching Hospital (HV-UEL) between April of 2005 and May of 2006. They presented anemia (Packed Cell Volume<25%), thrombocytopenia (Platelet count <150000/mm3), leukopenia (White blood cell count<5000/mm3) or a combination of two or three of these alterations at the moment of the consultation. The presence of Babesia sp was determined by the amplification of a specific fragment of DNA of the Babesia genus by PCR. Microscopic examination of Giemsa-stained blood smears detected 38 (13.5%) positive samples against 105 identified by PCR from 282 dogs. The positive samples were submitted to PCR-RFLP by Hinf I that allows distinguishing the species of B. canis vogeli and B. gibsoni. From 282 dogs, Babesia sp infection was identified in 105 (37.2%). From these 105 positive samples, the PCR-RFLP identified 66 (23.4%) samples with a profile compatible to B. canis vogeli and 39 (13, 8%) to B. gibsoni. As conclusions, the results obtained allow to affirm that the babesiose is an important differential for dogs that present anemia, leukopenia and thrombocytopenia and, B. canis vogeli is the subspecies that is present in the most of the cases of babesiose in the population of dogs studied and, that B. gibsoni is also present causing babesiosis in dogs of the Londrina region, Parana State, Brazil.</abstract><cop>Brazil</cop><pmid>20059862</pmid><tpages>7</tpages></addata></record> |
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| subjects | Animals Babesia - genetics Babesiosis - diagnosis Babesiosis - epidemiology Babesiosis - veterinary Brazil Dog Diseases - diagnosis Dog Diseases - epidemiology Dog Diseases - parasitology Dogs Hospitals, Animal Molecular Diagnostic Techniques |
| title | Occurrence and molecular characterization of Babesia species in a canine hospital population in the Londrina Region, Parana State, Brazil |
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