Zygocotyle lunata: Proteomic analysis of the adult stage
[Display omitted] ►The somatic extract of Zygocotyle lunata (Trematoda: Paramphistomidae) adults collected from experimentally infected mice was investigated. ► The largest protein family consisted of metabolic enzymes. ► Structural, motor and receptor binding proteins and proteins related to oxygen...
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| Veröffentlicht in: | Experimental parasitology 2011-06, Vol.128 (2), p.133-137 |
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| creator | Sotillo, Javier Valero, M. Luz Sánchez del Pino, Manuel M. Fried, Bernard Esteban, J. Guillermo Marcilla, Antonio Toledo, Rafael |
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►The somatic extract of Zygocotyle lunata (Trematoda: Paramphistomidae) adults collected from experimentally infected mice was investigated. ► The largest protein family consisted of metabolic enzymes. ► Structural, motor and receptor binding proteins and proteins related to oxygen transport were identified in the somatic extract of Z. lunata.
The somatic extract of Zygocotyle lunata (Trematoda: Paramphistomidae) adults collected from experimentally infected mice was investigated using a proteomic approach to separate and identify tryptic peptides from the somatic extract of Z. lunata adult worms. A shot-gun liquid chromatography/tandem mass spectrometry procedure was used. We used the MASCOT search engine (Matrix-Science) and ProteinPilot software v2.0 (Applied Biosystems) for the database search. A total of 36 proteins were accurately identified from the worms. The largest protein family consisted of metabolic enzymes. Structural, motor and receptor binding proteins and proteins related to oxygen transport were identified in the somatic extract of Z. lunata. This is the first study that attempts to identify the proteome of Z. lunata. However, more work is needed to improve our knowledge of trematodiasis in general and more specifically to have a better understanding about host–parasite relationships in infections with paramphistomes. |
| doi_str_mv | 10.1016/j.exppara.2011.02.014 |
| format | Article |
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►The somatic extract of Zygocotyle lunata (Trematoda: Paramphistomidae) adults collected from experimentally infected mice was investigated. ► The largest protein family consisted of metabolic enzymes. ► Structural, motor and receptor binding proteins and proteins related to oxygen transport were identified in the somatic extract of Z. lunata.
The somatic extract of Zygocotyle lunata (Trematoda: Paramphistomidae) adults collected from experimentally infected mice was investigated using a proteomic approach to separate and identify tryptic peptides from the somatic extract of Z. lunata adult worms. A shot-gun liquid chromatography/tandem mass spectrometry procedure was used. We used the MASCOT search engine (Matrix-Science) and ProteinPilot software v2.0 (Applied Biosystems) for the database search. A total of 36 proteins were accurately identified from the worms. The largest protein family consisted of metabolic enzymes. Structural, motor and receptor binding proteins and proteins related to oxygen transport were identified in the somatic extract of Z. lunata. This is the first study that attempts to identify the proteome of Z. lunata. However, more work is needed to improve our knowledge of trematodiasis in general and more specifically to have a better understanding about host–parasite relationships in infections with paramphistomes.</description><identifier>ISSN: 0014-4894</identifier><identifier>EISSN: 1090-2449</identifier><identifier>DOI: 10.1016/j.exppara.2011.02.014</identifier><identifier>PMID: 21334327</identifier><identifier>CODEN: EXPAAA</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>adults ; Animals ; binding proteins ; Biological and medical sciences ; Chromatography, Liquid ; computer software ; Databases, Protein ; enzymes ; Fundamental and applied biological sciences. Psychology ; Helminth Proteins - analysis ; Helminth Proteins - genetics ; Host-Parasite Interactions ; host-parasite relationships ; Invertebrates ; Life cycle. Host-agent relationship. Pathogenesis ; liquid chromatography ; Male ; Mass spectrometry ; Mice ; Mice, Inbred BALB C ; Nemathelminthia. Plathelmintha ; oxygen ; Paramphistomatidae - chemistry ; Paramphistomatidae - genetics ; Paramphistomatidae - physiology ; Paramphistomes ; Paramphistomidae ; parasitology ; peptides ; Proteome ; Proteome - analysis ; Proteome - genetics ; proteomics ; Protozoa ; Tandem Mass Spectrometry ; Trematoda ; Trematode Infections - parasitology ; Zygocotyle lunata</subject><ispartof>Experimental parasitology, 2011-06, Vol.128 (2), p.133-137</ispartof><rights>2011 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-df0e229e97fb9e8b939f112c0ee7855fb1ecd64708e3ccc89dd28dfd9303d76e3</citedby><cites>FETCH-LOGICAL-c418t-df0e229e97fb9e8b939f112c0ee7855fb1ecd64708e3ccc89dd28dfd9303d76e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.exppara.2011.02.014$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24107371$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21334327$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sotillo, Javier</creatorcontrib><creatorcontrib>Valero, M. Luz</creatorcontrib><creatorcontrib>Sánchez del Pino, Manuel M.</creatorcontrib><creatorcontrib>Fried, Bernard</creatorcontrib><creatorcontrib>Esteban, J. Guillermo</creatorcontrib><creatorcontrib>Marcilla, Antonio</creatorcontrib><creatorcontrib>Toledo, Rafael</creatorcontrib><title>Zygocotyle lunata: Proteomic analysis of the adult stage</title><title>Experimental parasitology</title><addtitle>Exp Parasitol</addtitle><description>[Display omitted]
►The somatic extract of Zygocotyle lunata (Trematoda: Paramphistomidae) adults collected from experimentally infected mice was investigated. ► The largest protein family consisted of metabolic enzymes. ► Structural, motor and receptor binding proteins and proteins related to oxygen transport were identified in the somatic extract of Z. lunata.
The somatic extract of Zygocotyle lunata (Trematoda: Paramphistomidae) adults collected from experimentally infected mice was investigated using a proteomic approach to separate and identify tryptic peptides from the somatic extract of Z. lunata adult worms. A shot-gun liquid chromatography/tandem mass spectrometry procedure was used. We used the MASCOT search engine (Matrix-Science) and ProteinPilot software v2.0 (Applied Biosystems) for the database search. A total of 36 proteins were accurately identified from the worms. The largest protein family consisted of metabolic enzymes. Structural, motor and receptor binding proteins and proteins related to oxygen transport were identified in the somatic extract of Z. lunata. This is the first study that attempts to identify the proteome of Z. lunata. However, more work is needed to improve our knowledge of trematodiasis in general and more specifically to have a better understanding about host–parasite relationships in infections with paramphistomes.</description><subject>adults</subject><subject>Animals</subject><subject>binding proteins</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Liquid</subject><subject>computer software</subject><subject>Databases, Protein</subject><subject>enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Helminth Proteins - analysis</subject><subject>Helminth Proteins - genetics</subject><subject>Host-Parasite Interactions</subject><subject>host-parasite relationships</subject><subject>Invertebrates</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>liquid chromatography</subject><subject>Male</subject><subject>Mass spectrometry</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Nemathelminthia. Plathelmintha</subject><subject>oxygen</subject><subject>Paramphistomatidae - chemistry</subject><subject>Paramphistomatidae - genetics</subject><subject>Paramphistomatidae - physiology</subject><subject>Paramphistomes</subject><subject>Paramphistomidae</subject><subject>parasitology</subject><subject>peptides</subject><subject>Proteome</subject><subject>Proteome - analysis</subject><subject>Proteome - genetics</subject><subject>proteomics</subject><subject>Protozoa</subject><subject>Tandem Mass Spectrometry</subject><subject>Trematoda</subject><subject>Trematode Infections - parasitology</subject><subject>Zygocotyle lunata</subject><issn>0014-4894</issn><issn>1090-2449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtvEzEQgC1ERUPhJ0D3gjjtMn4ka3OpUNWWSpWoBL1wsRx7HBxt1qntrZp_X1cJcOxppNE3r28I-UCho0AXX9YdPm63JpmOAaUdsA6oeEVmFBS0TAj1msygplohlTgmb3NeA4CkTLwhx4xyLjjrZ0T-3q2ijWU3YDNMoynma3ObYsG4CbYxoxl2OeQm-qb8wca4aShNLmaF78iRN0PG94d4Qu4uL36df29vflxdn3-7aa2gsrTOAzKmUPV-qVAuFVeeUmYBsZfzuV9StG4hepDIrbVSOcek805x4K5fID8hn_d9tyneT5iL3oRscRjMiHHKWi4YQL1RVnK-J22KOSf0epvCxqSdpqCfnem1PjjTz840MF0F1bqPhwnTcoPuX9VfSRX4dABMtmbwyYw25P-coNDznlbudM95E7VZpcrc_ayT5vUPPReMVeJsT2A19hAw6WwDjhZdSGiLdjG8sOwTx7yV4Q</recordid><startdate>20110601</startdate><enddate>20110601</enddate><creator>Sotillo, Javier</creator><creator>Valero, M. Luz</creator><creator>Sánchez del Pino, Manuel M.</creator><creator>Fried, Bernard</creator><creator>Esteban, J. Guillermo</creator><creator>Marcilla, Antonio</creator><creator>Toledo, Rafael</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110601</creationdate><title>Zygocotyle lunata: Proteomic analysis of the adult stage</title><author>Sotillo, Javier ; Valero, M. Luz ; Sánchez del Pino, Manuel M. ; Fried, Bernard ; Esteban, J. Guillermo ; Marcilla, Antonio ; Toledo, Rafael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-df0e229e97fb9e8b939f112c0ee7855fb1ecd64708e3ccc89dd28dfd9303d76e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>adults</topic><topic>Animals</topic><topic>binding proteins</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Liquid</topic><topic>computer software</topic><topic>Databases, Protein</topic><topic>enzymes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Helminth Proteins - analysis</topic><topic>Helminth Proteins - genetics</topic><topic>Host-Parasite Interactions</topic><topic>host-parasite relationships</topic><topic>Invertebrates</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>liquid chromatography</topic><topic>Male</topic><topic>Mass spectrometry</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Nemathelminthia. Plathelmintha</topic><topic>oxygen</topic><topic>Paramphistomatidae - chemistry</topic><topic>Paramphistomatidae - genetics</topic><topic>Paramphistomatidae - physiology</topic><topic>Paramphistomes</topic><topic>Paramphistomidae</topic><topic>parasitology</topic><topic>peptides</topic><topic>Proteome</topic><topic>Proteome - analysis</topic><topic>Proteome - genetics</topic><topic>proteomics</topic><topic>Protozoa</topic><topic>Tandem Mass Spectrometry</topic><topic>Trematoda</topic><topic>Trematode Infections - parasitology</topic><topic>Zygocotyle lunata</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sotillo, Javier</creatorcontrib><creatorcontrib>Valero, M. Luz</creatorcontrib><creatorcontrib>Sánchez del Pino, Manuel M.</creatorcontrib><creatorcontrib>Fried, Bernard</creatorcontrib><creatorcontrib>Esteban, J. Guillermo</creatorcontrib><creatorcontrib>Marcilla, Antonio</creatorcontrib><creatorcontrib>Toledo, Rafael</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sotillo, Javier</au><au>Valero, M. Luz</au><au>Sánchez del Pino, Manuel M.</au><au>Fried, Bernard</au><au>Esteban, J. Guillermo</au><au>Marcilla, Antonio</au><au>Toledo, Rafael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Zygocotyle lunata: Proteomic analysis of the adult stage</atitle><jtitle>Experimental parasitology</jtitle><addtitle>Exp Parasitol</addtitle><date>2011-06-01</date><risdate>2011</risdate><volume>128</volume><issue>2</issue><spage>133</spage><epage>137</epage><pages>133-137</pages><issn>0014-4894</issn><eissn>1090-2449</eissn><coden>EXPAAA</coden><abstract>[Display omitted]
►The somatic extract of Zygocotyle lunata (Trematoda: Paramphistomidae) adults collected from experimentally infected mice was investigated. ► The largest protein family consisted of metabolic enzymes. ► Structural, motor and receptor binding proteins and proteins related to oxygen transport were identified in the somatic extract of Z. lunata.
The somatic extract of Zygocotyle lunata (Trematoda: Paramphistomidae) adults collected from experimentally infected mice was investigated using a proteomic approach to separate and identify tryptic peptides from the somatic extract of Z. lunata adult worms. A shot-gun liquid chromatography/tandem mass spectrometry procedure was used. We used the MASCOT search engine (Matrix-Science) and ProteinPilot software v2.0 (Applied Biosystems) for the database search. A total of 36 proteins were accurately identified from the worms. The largest protein family consisted of metabolic enzymes. Structural, motor and receptor binding proteins and proteins related to oxygen transport were identified in the somatic extract of Z. lunata. This is the first study that attempts to identify the proteome of Z. lunata. However, more work is needed to improve our knowledge of trematodiasis in general and more specifically to have a better understanding about host–parasite relationships in infections with paramphistomes.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>21334327</pmid><doi>10.1016/j.exppara.2011.02.014</doi><tpages>5</tpages></addata></record> |
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| subjects | adults Animals binding proteins Biological and medical sciences Chromatography, Liquid computer software Databases, Protein enzymes Fundamental and applied biological sciences. Psychology Helminth Proteins - analysis Helminth Proteins - genetics Host-Parasite Interactions host-parasite relationships Invertebrates Life cycle. Host-agent relationship. Pathogenesis liquid chromatography Male Mass spectrometry Mice Mice, Inbred BALB C Nemathelminthia. Plathelmintha oxygen Paramphistomatidae - chemistry Paramphistomatidae - genetics Paramphistomatidae - physiology Paramphistomes Paramphistomidae parasitology peptides Proteome Proteome - analysis Proteome - genetics proteomics Protozoa Tandem Mass Spectrometry Trematoda Trematode Infections - parasitology Zygocotyle lunata |
| title | Zygocotyle lunata: Proteomic analysis of the adult stage |
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