Detection of HOXA9 gene methylation in tumor tissues and induced sputum samples from primary lung cancer patients
Background: Lung cancer is a leading cause of cancer deaths. Unfortunately, no effective early screening modality exists for lung cancer. We aimed to evaluate the prevalence of HOXA9 promoter methylation in tissue and induced sputum samples from Korean patients with lung cancer. Methods: Using pyros...
Gespeichert in:
| Veröffentlicht in: | Clinical chemistry and laboratory medicine 2011-04, Vol.49 (4), p.699-704 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 704 |
|---|---|
| container_issue | 4 |
| container_start_page | 699 |
| container_title | Clinical chemistry and laboratory medicine |
| container_volume | 49 |
| creator | Hwang, Sang-Hyun Kim, Ki Uk Kim, Ji-Eun Kim, Hyung-Hoi Lee, Min Ki Lee, Chang Hun Lee, Sang-Yull Oh, TaeJeong An, Sungwhan |
| description | Background: Lung cancer is a leading cause of cancer deaths. Unfortunately, no effective early screening modality exists for lung cancer. We aimed to evaluate the prevalence of HOXA9 promoter methylation in tissue and induced sputum samples from Korean patients with lung cancer. Methods: Using pyrosequencing, HOXA9 methylation was analyzed for 40 pairs of primary lung cancer and normal tissues and 185 induced sputum specimens, including 76 patients with lung cancer. Results: The methylation of HOXA9 in lung cancer tissue was significantly higher compared with normal tissues (67.4%±17.6% vs. 23.6%±10.3%, respectively; p45.6% of HOXA9 gene methylation in tissues, the sensitivity was 90.5% and the specificity was 97.5%. In induced sputum specimens, the HOXA9 gene in lung cancer patients was significantly more hypermethylated compared with patients with benign lung diseases and the healthy group (23.4%±15.9%, 14.9%±7.9%, and 9.7%±5.0%, respectively; p |
| doi_str_mv | 10.1515/CCLM.2011.108 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_861790149</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>861790149</sourcerecordid><originalsourceid>FETCH-LOGICAL-c459t-6e4b169b4ff722aab43db1affef0b1e80a6d49400e238b4d31700bb8fb2a3f1a3</originalsourceid><addsrcrecordid>eNp9kc1v1TAQxCMEoqVw5Ip8QfSShzfeOM6xCpSCHiqVisrNspN1CeSrtiPR_x6_9tHeONma-Wm868my18A3UEL5vmm2XzcFB9gAV0-yQ0BR5SgEPL27Yy5lAQfZixB-cQ5lidXz7KAAVFxBeZjdfKBIbeznic2OnZ3_OKnZNU3ERoo_bwdz5_QTi-s4exb7EFYKzExdEru1pY6FZU0mC2ZchmQ5P49s8f1o_C0b1umatWZqybMlZdEUw8vsmTNDoFf78yj7fvrxsjnLt-efPjcn27zFso65JLQga4vOVUVhjEXRWTDOkeMWSHEjO6yRcyqEstgJqDi3VjlbGOHAiKPs3X3u4uebNHTUYx9aGgYz0bwGrSRUNQesE3n8XxJQKiERiiqh-T3a-jkET07vV9XA9a4PvetD7_pIikr8m330akfqHuh_BSTg7R4woTWD8-mz-vDI4Y7C8vHhPkT68-Ab_1vLSlSlvrhE_U18uWrqqwstxV_fd6Lx</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1468364127</pqid></control><display><type>article</type><title>Detection of HOXA9 gene methylation in tumor tissues and induced sputum samples from primary lung cancer patients</title><source>MEDLINE</source><source>De Gruyter journals</source><creator>Hwang, Sang-Hyun ; Kim, Ki Uk ; Kim, Ji-Eun ; Kim, Hyung-Hoi ; Lee, Min Ki ; Lee, Chang Hun ; Lee, Sang-Yull ; Oh, TaeJeong ; An, Sungwhan</creator><creatorcontrib>Hwang, Sang-Hyun ; Kim, Ki Uk ; Kim, Ji-Eun ; Kim, Hyung-Hoi ; Lee, Min Ki ; Lee, Chang Hun ; Lee, Sang-Yull ; Oh, TaeJeong ; An, Sungwhan</creatorcontrib><description>Background: Lung cancer is a leading cause of cancer deaths. Unfortunately, no effective early screening modality exists for lung cancer. We aimed to evaluate the prevalence of HOXA9 promoter methylation in tissue and induced sputum samples from Korean patients with lung cancer. Methods: Using pyrosequencing, HOXA9 methylation was analyzed for 40 pairs of primary lung cancer and normal tissues and 185 induced sputum specimens, including 76 patients with lung cancer. Results: The methylation of HOXA9 in lung cancer tissue was significantly higher compared with normal tissues (67.4%±17.6% vs. 23.6%±10.3%, respectively; p<0.001). With a cut-off of >45.6% of HOXA9 gene methylation in tissues, the sensitivity was 90.5% and the specificity was 97.5%. In induced sputum specimens, the HOXA9 gene in lung cancer patients was significantly more hypermethylated compared with patients with benign lung diseases and the healthy group (23.4%±15.9%, 14.9%±7.9%, and 9.7%±5.0%, respectively; p<0.001). Conclusions: The HOXA9 gene was hypermethylated in 32 of 40 tumors (80%), especially in early stages of lung cancer. HOXA9 methylation could be a potential biomarker to aid early detection and prognosis.</description><identifier>ISSN: 1434-6621</identifier><identifier>EISSN: 1437-4331</identifier><identifier>DOI: 10.1515/CCLM.2011.108</identifier><identifier>PMID: 21480815</identifier><language>eng</language><publisher>Berlin: Walter de Gruyter</publisher><subject>Aged ; Base Sequence ; Benign ; Biological and medical sciences ; biomarker ; Case-Control Studies ; DNA methylation ; DNA Methylation - genetics ; early detection ; Female ; Gene Expression Regulation, Neoplastic ; General aspects ; Homeodomain Proteins - genetics ; HOXA9 gene ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Lung - cytology ; Lung - metabolism ; Lung - pathology ; lung cancer ; Lung Neoplasms - genetics ; Lung Neoplasms - pathology ; Male ; Medical sciences ; Middle Aged ; Pneumology ; Promoter Regions, Genetic - genetics ; Sputum - metabolism ; Tumors of the respiratory system and mediastinum</subject><ispartof>Clinical chemistry and laboratory medicine, 2011-04, Vol.49 (4), p.699-704</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-6e4b169b4ff722aab43db1affef0b1e80a6d49400e238b4d31700bb8fb2a3f1a3</citedby><cites>FETCH-LOGICAL-c459t-6e4b169b4ff722aab43db1affef0b1e80a6d49400e238b4d31700bb8fb2a3f1a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24081545$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21480815$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hwang, Sang-Hyun</creatorcontrib><creatorcontrib>Kim, Ki Uk</creatorcontrib><creatorcontrib>Kim, Ji-Eun</creatorcontrib><creatorcontrib>Kim, Hyung-Hoi</creatorcontrib><creatorcontrib>Lee, Min Ki</creatorcontrib><creatorcontrib>Lee, Chang Hun</creatorcontrib><creatorcontrib>Lee, Sang-Yull</creatorcontrib><creatorcontrib>Oh, TaeJeong</creatorcontrib><creatorcontrib>An, Sungwhan</creatorcontrib><title>Detection of HOXA9 gene methylation in tumor tissues and induced sputum samples from primary lung cancer patients</title><title>Clinical chemistry and laboratory medicine</title><addtitle>Clin Chem Lab Med</addtitle><description>Background: Lung cancer is a leading cause of cancer deaths. Unfortunately, no effective early screening modality exists for lung cancer. We aimed to evaluate the prevalence of HOXA9 promoter methylation in tissue and induced sputum samples from Korean patients with lung cancer. Methods: Using pyrosequencing, HOXA9 methylation was analyzed for 40 pairs of primary lung cancer and normal tissues and 185 induced sputum specimens, including 76 patients with lung cancer. Results: The methylation of HOXA9 in lung cancer tissue was significantly higher compared with normal tissues (67.4%±17.6% vs. 23.6%±10.3%, respectively; p<0.001). With a cut-off of >45.6% of HOXA9 gene methylation in tissues, the sensitivity was 90.5% and the specificity was 97.5%. In induced sputum specimens, the HOXA9 gene in lung cancer patients was significantly more hypermethylated compared with patients with benign lung diseases and the healthy group (23.4%±15.9%, 14.9%±7.9%, and 9.7%±5.0%, respectively; p<0.001). Conclusions: The HOXA9 gene was hypermethylated in 32 of 40 tumors (80%), especially in early stages of lung cancer. HOXA9 methylation could be a potential biomarker to aid early detection and prognosis.</description><subject>Aged</subject><subject>Base Sequence</subject><subject>Benign</subject><subject>Biological and medical sciences</subject><subject>biomarker</subject><subject>Case-Control Studies</subject><subject>DNA methylation</subject><subject>DNA Methylation - genetics</subject><subject>early detection</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>General aspects</subject><subject>Homeodomain Proteins - genetics</subject><subject>HOXA9 gene</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Lung - cytology</subject><subject>Lung - metabolism</subject><subject>Lung - pathology</subject><subject>lung cancer</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Pneumology</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Sputum - metabolism</subject><subject>Tumors of the respiratory system and mediastinum</subject><issn>1434-6621</issn><issn>1437-4331</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1v1TAQxCMEoqVw5Ip8QfSShzfeOM6xCpSCHiqVisrNspN1CeSrtiPR_x6_9tHeONma-Wm868my18A3UEL5vmm2XzcFB9gAV0-yQ0BR5SgEPL27Yy5lAQfZixB-cQ5lidXz7KAAVFxBeZjdfKBIbeznic2OnZ3_OKnZNU3ERoo_bwdz5_QTi-s4exb7EFYKzExdEru1pY6FZU0mC2ZchmQ5P49s8f1o_C0b1umatWZqybMlZdEUw8vsmTNDoFf78yj7fvrxsjnLt-efPjcn27zFso65JLQga4vOVUVhjEXRWTDOkeMWSHEjO6yRcyqEstgJqDi3VjlbGOHAiKPs3X3u4uebNHTUYx9aGgYz0bwGrSRUNQesE3n8XxJQKiERiiqh-T3a-jkET07vV9XA9a4PvetD7_pIikr8m330akfqHuh_BSTg7R4woTWD8-mz-vDI4Y7C8vHhPkT68-Ab_1vLSlSlvrhE_U18uWrqqwstxV_fd6Lx</recordid><startdate>20110401</startdate><enddate>20110401</enddate><creator>Hwang, Sang-Hyun</creator><creator>Kim, Ki Uk</creator><creator>Kim, Ji-Eun</creator><creator>Kim, Hyung-Hoi</creator><creator>Lee, Min Ki</creator><creator>Lee, Chang Hun</creator><creator>Lee, Sang-Yull</creator><creator>Oh, TaeJeong</creator><creator>An, Sungwhan</creator><general>Walter de Gruyter</general><general>De Gruyter</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20110401</creationdate><title>Detection of HOXA9 gene methylation in tumor tissues and induced sputum samples from primary lung cancer patients</title><author>Hwang, Sang-Hyun ; Kim, Ki Uk ; Kim, Ji-Eun ; Kim, Hyung-Hoi ; Lee, Min Ki ; Lee, Chang Hun ; Lee, Sang-Yull ; Oh, TaeJeong ; An, Sungwhan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-6e4b169b4ff722aab43db1affef0b1e80a6d49400e238b4d31700bb8fb2a3f1a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Aged</topic><topic>Base Sequence</topic><topic>Benign</topic><topic>Biological and medical sciences</topic><topic>biomarker</topic><topic>Case-Control Studies</topic><topic>DNA methylation</topic><topic>DNA Methylation - genetics</topic><topic>early detection</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>General aspects</topic><topic>Homeodomain Proteins - genetics</topic><topic>HOXA9 gene</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Lung - cytology</topic><topic>Lung - metabolism</topic><topic>Lung - pathology</topic><topic>lung cancer</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - pathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Pneumology</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Sputum - metabolism</topic><topic>Tumors of the respiratory system and mediastinum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hwang, Sang-Hyun</creatorcontrib><creatorcontrib>Kim, Ki Uk</creatorcontrib><creatorcontrib>Kim, Ji-Eun</creatorcontrib><creatorcontrib>Kim, Hyung-Hoi</creatorcontrib><creatorcontrib>Lee, Min Ki</creatorcontrib><creatorcontrib>Lee, Chang Hun</creatorcontrib><creatorcontrib>Lee, Sang-Yull</creatorcontrib><creatorcontrib>Oh, TaeJeong</creatorcontrib><creatorcontrib>An, Sungwhan</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical chemistry and laboratory medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hwang, Sang-Hyun</au><au>Kim, Ki Uk</au><au>Kim, Ji-Eun</au><au>Kim, Hyung-Hoi</au><au>Lee, Min Ki</au><au>Lee, Chang Hun</au><au>Lee, Sang-Yull</au><au>Oh, TaeJeong</au><au>An, Sungwhan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of HOXA9 gene methylation in tumor tissues and induced sputum samples from primary lung cancer patients</atitle><jtitle>Clinical chemistry and laboratory medicine</jtitle><addtitle>Clin Chem Lab Med</addtitle><date>2011-04-01</date><risdate>2011</risdate><volume>49</volume><issue>4</issue><spage>699</spage><epage>704</epage><pages>699-704</pages><issn>1434-6621</issn><eissn>1437-4331</eissn><abstract>Background: Lung cancer is a leading cause of cancer deaths. Unfortunately, no effective early screening modality exists for lung cancer. We aimed to evaluate the prevalence of HOXA9 promoter methylation in tissue and induced sputum samples from Korean patients with lung cancer. Methods: Using pyrosequencing, HOXA9 methylation was analyzed for 40 pairs of primary lung cancer and normal tissues and 185 induced sputum specimens, including 76 patients with lung cancer. Results: The methylation of HOXA9 in lung cancer tissue was significantly higher compared with normal tissues (67.4%±17.6% vs. 23.6%±10.3%, respectively; p<0.001). With a cut-off of >45.6% of HOXA9 gene methylation in tissues, the sensitivity was 90.5% and the specificity was 97.5%. In induced sputum specimens, the HOXA9 gene in lung cancer patients was significantly more hypermethylated compared with patients with benign lung diseases and the healthy group (23.4%±15.9%, 14.9%±7.9%, and 9.7%±5.0%, respectively; p<0.001). Conclusions: The HOXA9 gene was hypermethylated in 32 of 40 tumors (80%), especially in early stages of lung cancer. HOXA9 methylation could be a potential biomarker to aid early detection and prognosis.</abstract><cop>Berlin</cop><pub>Walter de Gruyter</pub><pmid>21480815</pmid><doi>10.1515/CCLM.2011.108</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1434-6621 |
| ispartof | Clinical chemistry and laboratory medicine, 2011-04, Vol.49 (4), p.699-704 |
| issn | 1434-6621 1437-4331 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_861790149 |
| source | MEDLINE; De Gruyter journals |
| subjects | Aged Base Sequence Benign Biological and medical sciences biomarker Case-Control Studies DNA methylation DNA Methylation - genetics early detection Female Gene Expression Regulation, Neoplastic General aspects Homeodomain Proteins - genetics HOXA9 gene Humans Investigative techniques, diagnostic techniques (general aspects) Lung - cytology Lung - metabolism Lung - pathology lung cancer Lung Neoplasms - genetics Lung Neoplasms - pathology Male Medical sciences Middle Aged Pneumology Promoter Regions, Genetic - genetics Sputum - metabolism Tumors of the respiratory system and mediastinum |
| title | Detection of HOXA9 gene methylation in tumor tissues and induced sputum samples from primary lung cancer patients |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T10%3A03%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Detection%20of%20HOXA9%20gene%20methylation%20in%20tumor%20tissues%20and%20induced%20sputum%20samples%20from%20primary%20lung%20cancer%20patients&rft.jtitle=Clinical%20chemistry%20and%20laboratory%20medicine&rft.au=Hwang,%20Sang-Hyun&rft.date=2011-04-01&rft.volume=49&rft.issue=4&rft.spage=699&rft.epage=704&rft.pages=699-704&rft.issn=1434-6621&rft.eissn=1437-4331&rft_id=info:doi/10.1515/CCLM.2011.108&rft_dat=%3Cproquest_cross%3E861790149%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1468364127&rft_id=info:pmid/21480815&rfr_iscdi=true |