A label-free and portable multichannel surface plasmon resonance immunosensor for on site analysis of antibiotics in milk samples
Techniques for immunosensing like surface plasmon resonance (SPR) may respond to the need for rapid screening methods to improve food safety. This paper describes the development of a novel portable six channel SPR biosensor based on the plasmon of gold diffraction grating surface for simultaneous m...
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Veröffentlicht in: | Biosensors & bioelectronics 2010-12, Vol.26 (4), p.1231-1238 |
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creator | Fernández, Fátima Hegnerová, Kateřina Piliarik, Marek Sanchez-Baeza, Francisco Homola, Jiří Marco, M.-Pilar |
description | Techniques for immunosensing like surface plasmon resonance (SPR) may respond to the need for rapid screening methods to improve food safety. This paper describes the development of a novel portable six channel SPR biosensor based on the plasmon of gold diffraction grating surface for simultaneous multianalyte antibiotic detection in milk samples. Representative congeners from three important antibiotic families (FQs: fluoroquinolones, SAs: sulfonamides and CAP: phenicols) were chosen for this study. The chips are covalently biofunctionalized with haptenized proteins by means of a previously formed mixed self assembled monolayer (m-SAM) prepared using two types of mercapto alkyl reagents containing polyethyleneglycol (PEG) units. The samples or standards are mixed with specific polyclonal antibodies and injected into the sensor device. The detectability accomplished is very good (i.e. in buffer, enrofloxacin, 0.30
μg
L
−1; sulfapyridine, 0.29
μg
L
−1; and chloramphenicol, 0.26
μg
L
−1) and whole milk samples can be analyzed directly without clean-up steps, by just diluting the sample five times with water to remove non-specific interferences caused by the matrix. Although the detectability of CAP regarding the MRPL (minimum required performance limit) is slightly compromised by the dilution, the detectability accomplished by FQs and SAs was far below the maximum residue levels (MRLs) established by the European Union. |
doi_str_mv | 10.1016/j.bios.2010.06.012 |
format | Article |
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μg
L
−1; sulfapyridine, 0.29
μg
L
−1; and chloramphenicol, 0.26
μg
L
−1) and whole milk samples can be analyzed directly without clean-up steps, by just diluting the sample five times with water to remove non-specific interferences caused by the matrix. Although the detectability of CAP regarding the MRPL (minimum required performance limit) is slightly compromised by the dilution, the detectability accomplished by FQs and SAs was far below the maximum residue levels (MRLs) established by the European Union.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2010.06.012</identifier><identifier>PMID: 20637590</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Animals ; Anti-Bacterial Agents - analysis ; Antibiotics ; Antibodies ; Biological and medical sciences ; Biosensors ; Biotechnology ; Chloramphenicol - analysis ; Equipment Design ; Fluoroquinolones - analysis ; Food Contamination - analysis ; Food Safety ; Fundamental and applied biological sciences. Psychology ; Hapten ; Lab-On-A-Chip Devices ; Methods. Procedures. Technologies ; Milk ; Milk - chemistry ; Multianalyte ; Portable biosensor ; Sulfapyridine - analysis ; Surface plasmon resonance ; Surface Plasmon Resonance - instrumentation ; Surface Plasmon Resonance - methods ; Surface Plasmon Resonance - statistics & numerical data ; Various methods and equipments</subject><ispartof>Biosensors & bioelectronics, 2010-12, Vol.26 (4), p.1231-1238</ispartof><rights>2010 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-b528e2127871b44cfe9839a6f75aa8521d1c9ec03a8286a787d2f49cdd0242283</citedby><cites>FETCH-LOGICAL-c417t-b528e2127871b44cfe9839a6f75aa8521d1c9ec03a8286a787d2f49cdd0242283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0956566310003131$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,3537,23909,23910,25118,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23909018$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20637590$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fernández, Fátima</creatorcontrib><creatorcontrib>Hegnerová, Kateřina</creatorcontrib><creatorcontrib>Piliarik, Marek</creatorcontrib><creatorcontrib>Sanchez-Baeza, Francisco</creatorcontrib><creatorcontrib>Homola, Jiří</creatorcontrib><creatorcontrib>Marco, M.-Pilar</creatorcontrib><title>A label-free and portable multichannel surface plasmon resonance immunosensor for on site analysis of antibiotics in milk samples</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>Techniques for immunosensing like surface plasmon resonance (SPR) may respond to the need for rapid screening methods to improve food safety. This paper describes the development of a novel portable six channel SPR biosensor based on the plasmon of gold diffraction grating surface for simultaneous multianalyte antibiotic detection in milk samples. Representative congeners from three important antibiotic families (FQs: fluoroquinolones, SAs: sulfonamides and CAP: phenicols) were chosen for this study. The chips are covalently biofunctionalized with haptenized proteins by means of a previously formed mixed self assembled monolayer (m-SAM) prepared using two types of mercapto alkyl reagents containing polyethyleneglycol (PEG) units. The samples or standards are mixed with specific polyclonal antibodies and injected into the sensor device. The detectability accomplished is very good (i.e. in buffer, enrofloxacin, 0.30
μg
L
−1; sulfapyridine, 0.29
μg
L
−1; and chloramphenicol, 0.26
μg
L
−1) and whole milk samples can be analyzed directly without clean-up steps, by just diluting the sample five times with water to remove non-specific interferences caused by the matrix. Although the detectability of CAP regarding the MRPL (minimum required performance limit) is slightly compromised by the dilution, the detectability accomplished by FQs and SAs was far below the maximum residue levels (MRLs) established by the European Union.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - analysis</subject><subject>Antibiotics</subject><subject>Antibodies</subject><subject>Biological and medical sciences</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Chloramphenicol - analysis</subject><subject>Equipment Design</subject><subject>Fluoroquinolones - analysis</subject><subject>Food Contamination - analysis</subject><subject>Food Safety</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hapten</subject><subject>Lab-On-A-Chip Devices</subject><subject>Methods. Procedures. Technologies</subject><subject>Milk</subject><subject>Milk - chemistry</subject><subject>Multianalyte</subject><subject>Portable biosensor</subject><subject>Sulfapyridine - analysis</subject><subject>Surface plasmon resonance</subject><subject>Surface Plasmon Resonance - instrumentation</subject><subject>Surface Plasmon Resonance - methods</subject><subject>Surface Plasmon Resonance - statistics & numerical data</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2P1SAUhonRONfRP-DCsDGueuWj0DZxM5n4lUziRteE0kPkSqFyWpNZ-s-luVfd6YJADg9vDuch5DlnR864fn06jiHjUbBaYPrIuHhADrzvZNMKqR6SAxuUbpTW8oo8QTwxxjo-sMfkSjAtOzWwA_l5Q6MdITa-AFCbJrrkstoxAp23uAb31aYEkeJWvHVAl2hxzokWwJxsqpUwz1vKCAlzob6ueoth3cNsvMeANPt6XkNttuYhDYnOIX6jaOclAj4lj7yNCM8u-zX58u7t59sPzd2n9x9vb-4a1_JubUYlehBcdH3Hx7Z1HoZeDlb7TlnbK8En7gZwTNpe9NpWbBK-Hdw0MdEK0ctr8uqcu5T8fQNczRzQQYw2Qd7Q9Jor2UrB_09yNWjVcV1JcSZdyYgFvFlKmG25N5yZ3ZE5md2R2R0Zpk11VB-9uMRv4wzTnye_pVTg5QWw6Gz0pc454F9ODmxgfP_RmzMHdWw_AhSDLkB1MoUCbjVTDv_q4xeqLbEb</recordid><startdate>20101215</startdate><enddate>20101215</enddate><creator>Fernández, Fátima</creator><creator>Hegnerová, Kateřina</creator><creator>Piliarik, Marek</creator><creator>Sanchez-Baeza, Francisco</creator><creator>Homola, Jiří</creator><creator>Marco, M.-Pilar</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20101215</creationdate><title>A label-free and portable multichannel surface plasmon resonance immunosensor for on site analysis of antibiotics in milk samples</title><author>Fernández, Fátima ; Hegnerová, Kateřina ; Piliarik, Marek ; Sanchez-Baeza, Francisco ; Homola, Jiří ; Marco, M.-Pilar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-b528e2127871b44cfe9839a6f75aa8521d1c9ec03a8286a787d2f49cdd0242283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - analysis</topic><topic>Antibiotics</topic><topic>Antibodies</topic><topic>Biological and medical sciences</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Chloramphenicol - analysis</topic><topic>Equipment Design</topic><topic>Fluoroquinolones - analysis</topic><topic>Food Contamination - analysis</topic><topic>Food Safety</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hapten</topic><topic>Lab-On-A-Chip Devices</topic><topic>Methods. Procedures. Technologies</topic><topic>Milk</topic><topic>Milk - chemistry</topic><topic>Multianalyte</topic><topic>Portable biosensor</topic><topic>Sulfapyridine - analysis</topic><topic>Surface plasmon resonance</topic><topic>Surface Plasmon Resonance - instrumentation</topic><topic>Surface Plasmon Resonance - methods</topic><topic>Surface Plasmon Resonance - statistics & numerical data</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fernández, Fátima</creatorcontrib><creatorcontrib>Hegnerová, Kateřina</creatorcontrib><creatorcontrib>Piliarik, Marek</creatorcontrib><creatorcontrib>Sanchez-Baeza, Francisco</creatorcontrib><creatorcontrib>Homola, Jiří</creatorcontrib><creatorcontrib>Marco, M.-Pilar</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fernández, Fátima</au><au>Hegnerová, Kateřina</au><au>Piliarik, Marek</au><au>Sanchez-Baeza, Francisco</au><au>Homola, Jiří</au><au>Marco, M.-Pilar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A label-free and portable multichannel surface plasmon resonance immunosensor for on site analysis of antibiotics in milk samples</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2010-12-15</date><risdate>2010</risdate><volume>26</volume><issue>4</issue><spage>1231</spage><epage>1238</epage><pages>1231-1238</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>Techniques for immunosensing like surface plasmon resonance (SPR) may respond to the need for rapid screening methods to improve food safety. This paper describes the development of a novel portable six channel SPR biosensor based on the plasmon of gold diffraction grating surface for simultaneous multianalyte antibiotic detection in milk samples. Representative congeners from three important antibiotic families (FQs: fluoroquinolones, SAs: sulfonamides and CAP: phenicols) were chosen for this study. The chips are covalently biofunctionalized with haptenized proteins by means of a previously formed mixed self assembled monolayer (m-SAM) prepared using two types of mercapto alkyl reagents containing polyethyleneglycol (PEG) units. The samples or standards are mixed with specific polyclonal antibodies and injected into the sensor device. The detectability accomplished is very good (i.e. in buffer, enrofloxacin, 0.30
μg
L
−1; sulfapyridine, 0.29
μg
L
−1; and chloramphenicol, 0.26
μg
L
−1) and whole milk samples can be analyzed directly without clean-up steps, by just diluting the sample five times with water to remove non-specific interferences caused by the matrix. Although the detectability of CAP regarding the MRPL (minimum required performance limit) is slightly compromised by the dilution, the detectability accomplished by FQs and SAs was far below the maximum residue levels (MRLs) established by the European Union.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>20637590</pmid><doi>10.1016/j.bios.2010.06.012</doi><tpages>8</tpages></addata></record> |
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source | MEDLINE; Elsevier ScienceDirect Journals |
subjects | Animals Anti-Bacterial Agents - analysis Antibiotics Antibodies Biological and medical sciences Biosensors Biotechnology Chloramphenicol - analysis Equipment Design Fluoroquinolones - analysis Food Contamination - analysis Food Safety Fundamental and applied biological sciences. Psychology Hapten Lab-On-A-Chip Devices Methods. Procedures. Technologies Milk Milk - chemistry Multianalyte Portable biosensor Sulfapyridine - analysis Surface plasmon resonance Surface Plasmon Resonance - instrumentation Surface Plasmon Resonance - methods Surface Plasmon Resonance - statistics & numerical data Various methods and equipments |
title | A label-free and portable multichannel surface plasmon resonance immunosensor for on site analysis of antibiotics in milk samples |
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