A label-free and portable multichannel surface plasmon resonance immunosensor for on site analysis of antibiotics in milk samples

Techniques for immunosensing like surface plasmon resonance (SPR) may respond to the need for rapid screening methods to improve food safety. This paper describes the development of a novel portable six channel SPR biosensor based on the plasmon of gold diffraction grating surface for simultaneous m...

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Veröffentlicht in:Biosensors & bioelectronics 2010-12, Vol.26 (4), p.1231-1238
Hauptverfasser: Fernández, Fátima, Hegnerová, Kateřina, Piliarik, Marek, Sanchez-Baeza, Francisco, Homola, Jiří, Marco, M.-Pilar
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container_issue 4
container_start_page 1231
container_title Biosensors & bioelectronics
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creator Fernández, Fátima
Hegnerová, Kateřina
Piliarik, Marek
Sanchez-Baeza, Francisco
Homola, Jiří
Marco, M.-Pilar
description Techniques for immunosensing like surface plasmon resonance (SPR) may respond to the need for rapid screening methods to improve food safety. This paper describes the development of a novel portable six channel SPR biosensor based on the plasmon of gold diffraction grating surface for simultaneous multianalyte antibiotic detection in milk samples. Representative congeners from three important antibiotic families (FQs: fluoroquinolones, SAs: sulfonamides and CAP: phenicols) were chosen for this study. The chips are covalently biofunctionalized with haptenized proteins by means of a previously formed mixed self assembled monolayer (m-SAM) prepared using two types of mercapto alkyl reagents containing polyethyleneglycol (PEG) units. The samples or standards are mixed with specific polyclonal antibodies and injected into the sensor device. The detectability accomplished is very good (i.e. in buffer, enrofloxacin, 0.30 μg L −1; sulfapyridine, 0.29 μg L −1; and chloramphenicol, 0.26 μg L −1) and whole milk samples can be analyzed directly without clean-up steps, by just diluting the sample five times with water to remove non-specific interferences caused by the matrix. Although the detectability of CAP regarding the MRPL (minimum required performance limit) is slightly compromised by the dilution, the detectability accomplished by FQs and SAs was far below the maximum residue levels (MRLs) established by the European Union.
doi_str_mv 10.1016/j.bios.2010.06.012
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Psychology</topic><topic>Hapten</topic><topic>Lab-On-A-Chip Devices</topic><topic>Methods. Procedures. Technologies</topic><topic>Milk</topic><topic>Milk - chemistry</topic><topic>Multianalyte</topic><topic>Portable biosensor</topic><topic>Sulfapyridine - analysis</topic><topic>Surface plasmon resonance</topic><topic>Surface Plasmon Resonance - instrumentation</topic><topic>Surface Plasmon Resonance - methods</topic><topic>Surface Plasmon Resonance - statistics &amp; numerical data</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fernández, Fátima</creatorcontrib><creatorcontrib>Hegnerová, Kateřina</creatorcontrib><creatorcontrib>Piliarik, Marek</creatorcontrib><creatorcontrib>Sanchez-Baeza, Francisco</creatorcontrib><creatorcontrib>Homola, Jiří</creatorcontrib><creatorcontrib>Marco, M.-Pilar</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biosensors &amp; bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fernández, Fátima</au><au>Hegnerová, Kateřina</au><au>Piliarik, Marek</au><au>Sanchez-Baeza, Francisco</au><au>Homola, Jiří</au><au>Marco, M.-Pilar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A label-free and portable multichannel surface plasmon resonance immunosensor for on site analysis of antibiotics in milk samples</atitle><jtitle>Biosensors &amp; bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2010-12-15</date><risdate>2010</risdate><volume>26</volume><issue>4</issue><spage>1231</spage><epage>1238</epage><pages>1231-1238</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>Techniques for immunosensing like surface plasmon resonance (SPR) may respond to the need for rapid screening methods to improve food safety. 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subjects Animals
Anti-Bacterial Agents - analysis
Antibiotics
Antibodies
Biological and medical sciences
Biosensors
Biotechnology
Chloramphenicol - analysis
Equipment Design
Fluoroquinolones - analysis
Food Contamination - analysis
Food Safety
Fundamental and applied biological sciences. Psychology
Hapten
Lab-On-A-Chip Devices
Methods. Procedures. Technologies
Milk
Milk - chemistry
Multianalyte
Portable biosensor
Sulfapyridine - analysis
Surface plasmon resonance
Surface Plasmon Resonance - instrumentation
Surface Plasmon Resonance - methods
Surface Plasmon Resonance - statistics & numerical data
Various methods and equipments
title A label-free and portable multichannel surface plasmon resonance immunosensor for on site analysis of antibiotics in milk samples
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