Biofilm forming multi drug resistant Staphylococcus spp. among patients with conjunctivitis
Biofilm forming multidrug resistant Staphylococcus spp. are major reservoirs for transmission of ophthalmic infections. They were isolated from ocular patients suffering from conjunctivitis. In this study we analyzed biofilm forming ability, antibiotic resistance profile of the Staphylococcus spp. i...
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| Veröffentlicht in: | Polish journal of microbiology 2010, Vol.59 (4), p.233-239 |
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| creator | Murugan, K Usha, M Malathi, P Al-Sohaibani, A Saleh Chandrasekaran, M |
| description | Biofilm forming multidrug resistant Staphylococcus spp. are major reservoirs for transmission of ophthalmic infections. They were isolated from ocular patients suffering from conjunctivitis. In this study we analyzed biofilm forming ability, antibiotic resistance profile of the Staphylococcus spp. isolated from clinical ocular patients, and their phylogenetic relationship with other community MRSA. Sixty Staphylococcus spp. strains isolated from clinical subjects were evaluated for their ability to form biofilm and express biofilm encoding ica gene. Among them 93% were slime producers and 87% were slime positive. Staphylococcus aureus and S. epidermidis were dominant strains among the isolates obtained from ocular patients. The strains also exhibited a differential biofilm formation quantitatively. Antibiotic susceptibility of the strains tested with Penicillin G, Ciprofloxacin, Ofloxacin, Methicillin, Amikacin, and Gentamicin indicated that they were resistant to more than one antibiotic. The amplicon of ica gene of strong biofilm producing S. aureus strains, obtained by polymerase chain reaction, was sequenced and their close genetic relationship with community acquired MRSA was analyzed based on phylogenetic tree. Our results indicate that they are genetically close to other community acquired MRSA. |
| doi_str_mv | 10.33073/pjm-2010-036 |
| format | Article |
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They were isolated from ocular patients suffering from conjunctivitis. In this study we analyzed biofilm forming ability, antibiotic resistance profile of the Staphylococcus spp. isolated from clinical ocular patients, and their phylogenetic relationship with other community MRSA. Sixty Staphylococcus spp. strains isolated from clinical subjects were evaluated for their ability to form biofilm and express biofilm encoding ica gene. Among them 93% were slime producers and 87% were slime positive. Staphylococcus aureus and S. epidermidis were dominant strains among the isolates obtained from ocular patients. The strains also exhibited a differential biofilm formation quantitatively. Antibiotic susceptibility of the strains tested with Penicillin G, Ciprofloxacin, Ofloxacin, Methicillin, Amikacin, and Gentamicin indicated that they were resistant to more than one antibiotic. The amplicon of ica gene of strong biofilm producing S. aureus strains, obtained by polymerase chain reaction, was sequenced and their close genetic relationship with community acquired MRSA was analyzed based on phylogenetic tree. Our results indicate that they are genetically close to other community acquired MRSA.</description><identifier>ISSN: 1733-1331</identifier><identifier>EISSN: 2544-4646</identifier><identifier>DOI: 10.33073/pjm-2010-036</identifier><identifier>PMID: 21466040</identifier><language>eng</language><publisher>Poland: De Gruyter Poland</publisher><subject>Anti-Bacterial Agents - pharmacology ; Bacterial Adhesion - genetics ; Base Sequence ; Biofilms ; Biofilms - growth & development ; Community-Acquired Infections - microbiology ; Conjunctivitis ; Conjunctivitis - microbiology ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Bacterial - isolation & purification ; Drug resistance ; Drug Resistance, Multiple, Bacterial ; Female ; Humans ; Male ; Methicillin-Resistant Staphylococcus aureus - drug effects ; Methicillin-Resistant Staphylococcus aureus - pathogenicity ; Methicillin-Resistant Staphylococcus aureus - physiology ; Microbial Sensitivity Tests ; Molecular Sequence Data ; Phylogeny ; Polymerase Chain Reaction ; Sequence Analysis, DNA ; Staphylococcal Infections - microbiology ; Staphylococcus - drug effects ; Staphylococcus - pathogenicity ; Staphylococcus - physiology ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - pathogenicity ; Staphylococcus aureus - physiology ; Staphylococcus epidermidis - drug effects ; Staphylococcus epidermidis - pathogenicity ; Staphylococcus epidermidis - physiology</subject><ispartof>Polish journal of microbiology, 2010, Vol.59 (4), p.233-239</ispartof><rights>2010. 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They were isolated from ocular patients suffering from conjunctivitis. In this study we analyzed biofilm forming ability, antibiotic resistance profile of the Staphylococcus spp. isolated from clinical ocular patients, and their phylogenetic relationship with other community MRSA. Sixty Staphylococcus spp. strains isolated from clinical subjects were evaluated for their ability to form biofilm and express biofilm encoding ica gene. Among them 93% were slime producers and 87% were slime positive. Staphylococcus aureus and S. epidermidis were dominant strains among the isolates obtained from ocular patients. The strains also exhibited a differential biofilm formation quantitatively. Antibiotic susceptibility of the strains tested with Penicillin G, Ciprofloxacin, Ofloxacin, Methicillin, Amikacin, and Gentamicin indicated that they were resistant to more than one antibiotic. The amplicon of ica gene of strong biofilm producing S. aureus strains, obtained by polymerase chain reaction, was sequenced and their close genetic relationship with community acquired MRSA was analyzed based on phylogenetic tree. 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Usha, M ; Malathi, P ; Al-Sohaibani, A Saleh ; Chandrasekaran, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-7c9d67edb3318ed9771ac88b338e5fd4e50bd12d87c006f11b881caccf38d3ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Bacterial Adhesion - genetics</topic><topic>Base Sequence</topic><topic>Biofilms</topic><topic>Biofilms - growth & development</topic><topic>Community-Acquired Infections - microbiology</topic><topic>Conjunctivitis</topic><topic>Conjunctivitis - microbiology</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>Drug resistance</topic><topic>Drug Resistance, Multiple, Bacterial</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Methicillin-Resistant Staphylococcus aureus - drug effects</topic><topic>Methicillin-Resistant Staphylococcus aureus - pathogenicity</topic><topic>Methicillin-Resistant Staphylococcus aureus - physiology</topic><topic>Microbial Sensitivity Tests</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction</topic><topic>Sequence Analysis, DNA</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcus - drug effects</topic><topic>Staphylococcus - pathogenicity</topic><topic>Staphylococcus - physiology</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - pathogenicity</topic><topic>Staphylococcus aureus - physiology</topic><topic>Staphylococcus epidermidis - drug effects</topic><topic>Staphylococcus epidermidis - pathogenicity</topic><topic>Staphylococcus epidermidis - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Murugan, K</creatorcontrib><creatorcontrib>Usha, M</creatorcontrib><creatorcontrib>Malathi, P</creatorcontrib><creatorcontrib>Al-Sohaibani, A Saleh</creatorcontrib><creatorcontrib>Chandrasekaran, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Polish journal of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Murugan, K</au><au>Usha, M</au><au>Malathi, P</au><au>Al-Sohaibani, A Saleh</au><au>Chandrasekaran, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biofilm forming multi drug resistant Staphylococcus spp. among patients with conjunctivitis</atitle><jtitle>Polish journal of microbiology</jtitle><addtitle>Pol J Microbiol</addtitle><date>2010</date><risdate>2010</risdate><volume>59</volume><issue>4</issue><spage>233</spage><epage>239</epage><pages>233-239</pages><issn>1733-1331</issn><eissn>2544-4646</eissn><abstract>Biofilm forming multidrug resistant Staphylococcus spp. are major reservoirs for transmission of ophthalmic infections. They were isolated from ocular patients suffering from conjunctivitis. In this study we analyzed biofilm forming ability, antibiotic resistance profile of the Staphylococcus spp. isolated from clinical ocular patients, and their phylogenetic relationship with other community MRSA. Sixty Staphylococcus spp. strains isolated from clinical subjects were evaluated for their ability to form biofilm and express biofilm encoding ica gene. Among them 93% were slime producers and 87% were slime positive. Staphylococcus aureus and S. epidermidis were dominant strains among the isolates obtained from ocular patients. The strains also exhibited a differential biofilm formation quantitatively. Antibiotic susceptibility of the strains tested with Penicillin G, Ciprofloxacin, Ofloxacin, Methicillin, Amikacin, and Gentamicin indicated that they were resistant to more than one antibiotic. The amplicon of ica gene of strong biofilm producing S. aureus strains, obtained by polymerase chain reaction, was sequenced and their close genetic relationship with community acquired MRSA was analyzed based on phylogenetic tree. Our results indicate that they are genetically close to other community acquired MRSA.</abstract><cop>Poland</cop><pub>De Gruyter Poland</pub><pmid>21466040</pmid><doi>10.33073/pjm-2010-036</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anti-Bacterial Agents - pharmacology Bacterial Adhesion - genetics Base Sequence Biofilms Biofilms - growth & development Community-Acquired Infections - microbiology Conjunctivitis Conjunctivitis - microbiology DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Drug resistance Drug Resistance, Multiple, Bacterial Female Humans Male Methicillin-Resistant Staphylococcus aureus - drug effects Methicillin-Resistant Staphylococcus aureus - pathogenicity Methicillin-Resistant Staphylococcus aureus - physiology Microbial Sensitivity Tests Molecular Sequence Data Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA Staphylococcal Infections - microbiology Staphylococcus - drug effects Staphylococcus - pathogenicity Staphylococcus - physiology Staphylococcus aureus - drug effects Staphylococcus aureus - pathogenicity Staphylococcus aureus - physiology Staphylococcus epidermidis - drug effects Staphylococcus epidermidis - pathogenicity Staphylococcus epidermidis - physiology |
| title | Biofilm forming multi drug resistant Staphylococcus spp. among patients with conjunctivitis |
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