Dysbiosis of the faecal microbiota in patients with Crohn's disease and their unaffected relatives
Background and aimsA general dysbiosis of the intestinal microbiota has been established in patients with Crohn's disease (CD), but a systematic characterisation of this dysbiosis is lacking. Therefore the composition of the predominant faecal microbiota of patients with CD was studied in compa...
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| Veröffentlicht in: | Gut 2011-05, Vol.60 (5), p.631-637 |
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| description | Background and aimsA general dysbiosis of the intestinal microbiota has been established in patients with Crohn's disease (CD), but a systematic characterisation of this dysbiosis is lacking. Therefore the composition of the predominant faecal microbiota of patients with CD was studied in comparison with the predominant composition in unaffected controls. Whether dysbiosis is present in relatives of patients CD was also examined.MethodsFocusing on families with at least three members affected with CD, faecal samples of 68 patients with CD, 84 of their unaffected relatives and 55 matched controls were subjected to community fingerprinting of the predominant microbiota using denaturing gradient gel electrophoresis (DGGE). To analyse the DGGE profiles, BioNumerics software and non-parametric statistical analyses (SPSS V.17.0) were used. Observed differences in the predominant microbiota were subsequently confirmed and quantified with real-time PCR.ResultsFive bacterial species characterised dysbiosis in CD, namely a decrease in Dialister invisus (p=0.04), an uncharacterised species of Clostridium cluster XIVa (p=0.03), Faecalibacterium prausnitzii (p |
| doi_str_mv | 10.1136/gut.2010.223263 |
| format | Article |
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Therefore the composition of the predominant faecal microbiota of patients with CD was studied in comparison with the predominant composition in unaffected controls. Whether dysbiosis is present in relatives of patients CD was also examined.MethodsFocusing on families with at least three members affected with CD, faecal samples of 68 patients with CD, 84 of their unaffected relatives and 55 matched controls were subjected to community fingerprinting of the predominant microbiota using denaturing gradient gel electrophoresis (DGGE). To analyse the DGGE profiles, BioNumerics software and non-parametric statistical analyses (SPSS V.17.0) were used. Observed differences in the predominant microbiota were subsequently confirmed and quantified with real-time PCR.ResultsFive bacterial species characterised dysbiosis in CD, namely a decrease in Dialister invisus (p=0.04), an uncharacterised species of Clostridium cluster XIVa (p=0.03), Faecalibacterium prausnitzii (p<1.3×10−5) and Bifidobacterium adolescentis (p=5.4×10−6), and an increase in Ruminococcus gnavus (p=2.1×10−7). Unaffected relatives of patients with CD had less Collinsella aerofaciens (p=0.004) and a member of the Escherichia coli–Shigella group (p=0.01) and more Ruminococcus torques (p=0.02) in their predominant microbiota as compared with healthy subjects.ConclusionUnaffected relatives of patients with CD have a different composition of their microbiota compared with healthy controls. This dysbiosis is not characterised by lack of butyrate producing-bacteria as observed in CD but suggests a role for microorganisms with mucin degradation capacity.</description><identifier>ISSN: 0017-5749</identifier><identifier>EISSN: 1468-3288</identifier><identifier>DOI: 10.1136/gut.2010.223263</identifier><identifier>PMID: 21209126</identifier><identifier>CODEN: GUTTAK</identifier><language>eng</language><publisher>London: BMJ Publishing Group Ltd and British Society of Gastroenterology</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Autophagy ; Bacteria ; Bacteria - classification ; Bacteria - isolation & purification ; Bifidobacterium adolescentis ; Biological and medical sciences ; Biopsy ; Case-Control Studies ; Clostridium ; Collinsella aerofaciens ; Crohn Disease - genetics ; Crohn Disease - microbiology ; Crohn's disease ; Crohns disease ; Denaturing Gradient Gel Electrophoresis - methods ; DGGE ; DNA, Bacterial - analysis ; dysbiosis ; Escherichia ; Faecalibacterium prausnitzii ; Feces - microbiology ; Female ; Gastroenterology. Liver. Pancreas. Abdomen ; Genotype & phenotype ; Humans ; Inflammatory bowel disease ; Irritable bowel syndrome ; Male ; Medical sciences ; Metagenome ; Middle Aged ; mucin degradation ; Other diseases. Semiology ; Reverse Transcriptase Polymerase Chain Reaction - methods ; Ruminococcus ; Ruminococcus gnavus ; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus ; Studies ; Symbiosis - genetics ; Young Adult</subject><ispartof>Gut, 2011-05, Vol.60 (5), p.631-637</ispartof><rights>2011, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright: 2011 (c) 2011, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b506t-c07131de31f27d0f220294e9e648a5078bc3d1ebe8d4701b28a551aeba5e60fa3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttp://gut.bmj.com/content/60/5/631.full.pdf$$EPDF$$P50$$Gbmj$$H</linktopdf><linktohtml>$$Uhttp://gut.bmj.com/content/60/5/631.full$$EHTML$$P50$$Gbmj$$H</linktohtml><link.rule.ids>114,115,315,781,785,3197,23576,27929,27930,77605,77636</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24066568$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21209126$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Joossens, Marie</creatorcontrib><creatorcontrib>Huys, Geert</creatorcontrib><creatorcontrib>Cnockaert, Margo</creatorcontrib><creatorcontrib>De Preter, Vicky</creatorcontrib><creatorcontrib>Verbeke, Kristin</creatorcontrib><creatorcontrib>Rutgeerts, Paul</creatorcontrib><creatorcontrib>Vandamme, Peter</creatorcontrib><creatorcontrib>Vermeire, Severine</creatorcontrib><title>Dysbiosis of the faecal microbiota in patients with Crohn's disease and their unaffected relatives</title><title>Gut</title><addtitle>Gut</addtitle><description>Background and aimsA general dysbiosis of the intestinal microbiota has been established in patients with Crohn's disease (CD), but a systematic characterisation of this dysbiosis is lacking. Therefore the composition of the predominant faecal microbiota of patients with CD was studied in comparison with the predominant composition in unaffected controls. Whether dysbiosis is present in relatives of patients CD was also examined.MethodsFocusing on families with at least three members affected with CD, faecal samples of 68 patients with CD, 84 of their unaffected relatives and 55 matched controls were subjected to community fingerprinting of the predominant microbiota using denaturing gradient gel electrophoresis (DGGE). To analyse the DGGE profiles, BioNumerics software and non-parametric statistical analyses (SPSS V.17.0) were used. Observed differences in the predominant microbiota were subsequently confirmed and quantified with real-time PCR.ResultsFive bacterial species characterised dysbiosis in CD, namely a decrease in Dialister invisus (p=0.04), an uncharacterised species of Clostridium cluster XIVa (p=0.03), Faecalibacterium prausnitzii (p<1.3×10−5) and Bifidobacterium adolescentis (p=5.4×10−6), and an increase in Ruminococcus gnavus (p=2.1×10−7). Unaffected relatives of patients with CD had less Collinsella aerofaciens (p=0.004) and a member of the Escherichia coli–Shigella group (p=0.01) and more Ruminococcus torques (p=0.02) in their predominant microbiota as compared with healthy subjects.ConclusionUnaffected relatives of patients with CD have a different composition of their microbiota compared with healthy controls. This dysbiosis is not characterised by lack of butyrate producing-bacteria as observed in CD but suggests a role for microorganisms with mucin degradation capacity.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Autophagy</subject><subject>Bacteria</subject><subject>Bacteria - classification</subject><subject>Bacteria - isolation & purification</subject><subject>Bifidobacterium adolescentis</subject><subject>Biological and medical sciences</subject><subject>Biopsy</subject><subject>Case-Control Studies</subject><subject>Clostridium</subject><subject>Collinsella aerofaciens</subject><subject>Crohn Disease - genetics</subject><subject>Crohn Disease - microbiology</subject><subject>Crohn's disease</subject><subject>Crohns disease</subject><subject>Denaturing Gradient Gel Electrophoresis - methods</subject><subject>DGGE</subject><subject>DNA, Bacterial - analysis</subject><subject>dysbiosis</subject><subject>Escherichia</subject><subject>Faecalibacterium prausnitzii</subject><subject>Feces - microbiology</subject><subject>Female</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Genotype & phenotype</subject><subject>Humans</subject><subject>Inflammatory bowel disease</subject><subject>Irritable bowel syndrome</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Metagenome</subject><subject>Middle Aged</subject><subject>mucin degradation</subject><subject>Other diseases. Semiology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>Ruminococcus</subject><subject>Ruminococcus gnavus</subject><subject>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</subject><subject>Studies</subject><subject>Symbiosis - genetics</subject><subject>Young Adult</subject><issn>0017-5749</issn><issn>1468-3288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqF0ctv1DAQB-AIgehSOHNDlhAqQko7tuNHjmgpBVHgAoib5SRj1kseW9sp9L_HqyxF4gAny55v_PoVxWMKp5RyefZtTqcM8owxziS_U6xoJXXJmdZ3ixUAVaVQVX1UPIhxCwBa1_R-ccQog5oyuSqaVzex8VP0kUyOpA0SZ7G1PRl8G6ZcSZb4kexs8jimSH74tCHrMG3Gk0g6H9FGJHbs9q0-kHm0zmGbsCMB-9x0jfFhcc_ZPuKjw3hcfH59_mn9prz8ePF2_fKybATIVLagKKcdcuqY6sAxBqyusEZZaStA6ablHcUGdVcpoA3Lq4JabKxACc7y4-Jk2XcXpqsZYzKDjy32vR1xmqPREnitRC2yfP5PSYUEypQElenTv-h2msOY32GoUjUXoAXP6mxR-c9iDOjMLvjBhhtDweyDMjkosw_KLEHljieHfedmwO7W_04mg2cHYGPOwwU7tj7-cRVIKaTOrlycjwl_3tZt-G6k4kqYD1_W5uuF4Oz9u3x69i8W3wzb_97yF3aPtok</recordid><startdate>20110501</startdate><enddate>20110501</enddate><creator>Joossens, Marie</creator><creator>Huys, Geert</creator><creator>Cnockaert, Margo</creator><creator>De Preter, Vicky</creator><creator>Verbeke, Kristin</creator><creator>Rutgeerts, Paul</creator><creator>Vandamme, Peter</creator><creator>Vermeire, Severine</creator><general>BMJ Publishing Group Ltd and British Society of Gastroenterology</general><general>BMJ Publishing Group</general><general>BMJ Publishing Group LTD</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7T5</scope><scope>C1K</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20110501</creationdate><title>Dysbiosis of the faecal microbiota in patients with Crohn's disease and their unaffected relatives</title><author>Joossens, Marie ; Huys, Geert ; Cnockaert, Margo ; De Preter, Vicky ; Verbeke, Kristin ; Rutgeerts, Paul ; Vandamme, Peter ; Vermeire, Severine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b506t-c07131de31f27d0f220294e9e648a5078bc3d1ebe8d4701b28a551aeba5e60fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Autophagy</topic><topic>Bacteria</topic><topic>Bacteria - classification</topic><topic>Bacteria - isolation & purification</topic><topic>Bifidobacterium adolescentis</topic><topic>Biological and medical sciences</topic><topic>Biopsy</topic><topic>Case-Control Studies</topic><topic>Clostridium</topic><topic>Collinsella aerofaciens</topic><topic>Crohn Disease - genetics</topic><topic>Crohn Disease - microbiology</topic><topic>Crohn's disease</topic><topic>Crohns disease</topic><topic>Denaturing Gradient Gel Electrophoresis - methods</topic><topic>DGGE</topic><topic>DNA, Bacterial - analysis</topic><topic>dysbiosis</topic><topic>Escherichia</topic><topic>Faecalibacterium prausnitzii</topic><topic>Feces - microbiology</topic><topic>Female</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Genotype & phenotype</topic><topic>Humans</topic><topic>Inflammatory bowel disease</topic><topic>Irritable bowel syndrome</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Metagenome</topic><topic>Middle Aged</topic><topic>mucin degradation</topic><topic>Other diseases. Semiology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>Ruminococcus</topic><topic>Ruminococcus gnavus</topic><topic>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</topic><topic>Studies</topic><topic>Symbiosis - genetics</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Joossens, Marie</creatorcontrib><creatorcontrib>Huys, Geert</creatorcontrib><creatorcontrib>Cnockaert, Margo</creatorcontrib><creatorcontrib>De Preter, Vicky</creatorcontrib><creatorcontrib>Verbeke, Kristin</creatorcontrib><creatorcontrib>Rutgeerts, Paul</creatorcontrib><creatorcontrib>Vandamme, Peter</creatorcontrib><creatorcontrib>Vermeire, Severine</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>Proquest Central</collection><collection>Natural Science Collection</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database (ProQuest)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Gut</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Joossens, Marie</au><au>Huys, Geert</au><au>Cnockaert, Margo</au><au>De Preter, Vicky</au><au>Verbeke, Kristin</au><au>Rutgeerts, Paul</au><au>Vandamme, Peter</au><au>Vermeire, Severine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dysbiosis of the faecal microbiota in patients with Crohn's disease and their unaffected relatives</atitle><jtitle>Gut</jtitle><addtitle>Gut</addtitle><date>2011-05-01</date><risdate>2011</risdate><volume>60</volume><issue>5</issue><spage>631</spage><epage>637</epage><pages>631-637</pages><issn>0017-5749</issn><eissn>1468-3288</eissn><coden>GUTTAK</coden><abstract>Background and aimsA general dysbiosis of the intestinal microbiota has been established in patients with Crohn's disease (CD), but a systematic characterisation of this dysbiosis is lacking. Therefore the composition of the predominant faecal microbiota of patients with CD was studied in comparison with the predominant composition in unaffected controls. Whether dysbiosis is present in relatives of patients CD was also examined.MethodsFocusing on families with at least three members affected with CD, faecal samples of 68 patients with CD, 84 of their unaffected relatives and 55 matched controls were subjected to community fingerprinting of the predominant microbiota using denaturing gradient gel electrophoresis (DGGE). To analyse the DGGE profiles, BioNumerics software and non-parametric statistical analyses (SPSS V.17.0) were used. Observed differences in the predominant microbiota were subsequently confirmed and quantified with real-time PCR.ResultsFive bacterial species characterised dysbiosis in CD, namely a decrease in Dialister invisus (p=0.04), an uncharacterised species of Clostridium cluster XIVa (p=0.03), Faecalibacterium prausnitzii (p<1.3×10−5) and Bifidobacterium adolescentis (p=5.4×10−6), and an increase in Ruminococcus gnavus (p=2.1×10−7). Unaffected relatives of patients with CD had less Collinsella aerofaciens (p=0.004) and a member of the Escherichia coli–Shigella group (p=0.01) and more Ruminococcus torques (p=0.02) in their predominant microbiota as compared with healthy subjects.ConclusionUnaffected relatives of patients with CD have a different composition of their microbiota compared with healthy controls. This dysbiosis is not characterised by lack of butyrate producing-bacteria as observed in CD but suggests a role for microorganisms with mucin degradation capacity.</abstract><cop>London</cop><pub>BMJ Publishing Group Ltd and British Society of Gastroenterology</pub><pmid>21209126</pmid><doi>10.1136/gut.2010.223263</doi><tpages>7</tpages></addata></record> |
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| subjects | Adolescent Adult Aged Aged, 80 and over Autophagy Bacteria Bacteria - classification Bacteria - isolation & purification Bifidobacterium adolescentis Biological and medical sciences Biopsy Case-Control Studies Clostridium Collinsella aerofaciens Crohn Disease - genetics Crohn Disease - microbiology Crohn's disease Crohns disease Denaturing Gradient Gel Electrophoresis - methods DGGE DNA, Bacterial - analysis dysbiosis Escherichia Faecalibacterium prausnitzii Feces - microbiology Female Gastroenterology. Liver. Pancreas. Abdomen Genotype & phenotype Humans Inflammatory bowel disease Irritable bowel syndrome Male Medical sciences Metagenome Middle Aged mucin degradation Other diseases. Semiology Reverse Transcriptase Polymerase Chain Reaction - methods Ruminococcus Ruminococcus gnavus Stomach. Duodenum. Small intestine. Colon. Rectum. Anus Studies Symbiosis - genetics Young Adult |
| title | Dysbiosis of the faecal microbiota in patients with Crohn's disease and their unaffected relatives |
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