Increased resistance to fusarium wilt in transgenic tobacco lines co-expressing chitinase and wasabi defensin genes
Marker-free transgenic tobacco (Nicotiana tabacum) lines containing a chitinase (ChiC) gene isolated from Streptomyces griseus strain HUT 6037 were produced by Agrobacterium-mediated transformation. One marker-free transgenic line, TC-1, was retransformed with the wasabi defensin (WD) gene, isolated...
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| Veröffentlicht in: | Plant pathology 2011-04, Vol.60 (2), p.221-231 |
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| creator | Ntui, V.O Azadi, P Thirukkumaran, G Khan, R.S Chin, D.P Nakamura, I Mii, M |
| description | Marker-free transgenic tobacco (Nicotiana tabacum) lines containing a chitinase (ChiC) gene isolated from Streptomyces griseus strain HUT 6037 were produced by Agrobacterium-mediated transformation. One marker-free transgenic line, TC-1, was retransformed with the wasabi defensin (WD) gene, isolated from Wasabia japonica. Of the retransformed shoots, 37% co-expressed the ChiC/WD genes, as confirmed by western and northern analyses. Southern blot analysis showed that no chromosomal rearrangement was introduced between the first and the second transformation. Transgenic lines either expressing ChiC or WD, or co-expressing both genes were challenged with Fusarium oxysporum f.sp. nicotianae (Fon). Assessment of in vitro plant survival in the presence of Fon showed that transgenic lines co-expressing both genes had significantly enhanced protection against the fungus (infection indices 0·0-1.·2) compared with corresponding isogenic lines expressing either of the genes (infection indices 2·5-9·8). Whole-plant infection indices in transgenic lines were significantly related (r = 0·93, P < 0·01) to the extent of root colonization of the host, which ranged from 2·1% to 11·3% in lines co-expressing both genes, and from 16·8% to 37·7% in lines expressing just one of the genes (compared with 86·4% in non-transformed controls). Leaf extracts of transgenic lines also inhibited mycelial growth of Fon in vitro and caused hyphal abnormalities. |
| doi_str_mv | 10.1111/j.1365-3059.2010.02352.x |
| format | Article |
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One marker-free transgenic line, TC-1, was retransformed with the wasabi defensin (WD) gene, isolated from Wasabia japonica. Of the retransformed shoots, 37% co-expressed the ChiC/WD genes, as confirmed by western and northern analyses. Southern blot analysis showed that no chromosomal rearrangement was introduced between the first and the second transformation. Transgenic lines either expressing ChiC or WD, or co-expressing both genes were challenged with Fusarium oxysporum f.sp. nicotianae (Fon). Assessment of in vitro plant survival in the presence of Fon showed that transgenic lines co-expressing both genes had significantly enhanced protection against the fungus (infection indices 0·0-1.·2) compared with corresponding isogenic lines expressing either of the genes (infection indices 2·5-9·8). Whole-plant infection indices in transgenic lines were significantly related (r = 0·93, P < 0·01) to the extent of root colonization of the host, which ranged from 2·1% to 11·3% in lines co-expressing both genes, and from 16·8% to 37·7% in lines expressing just one of the genes (compared with 86·4% in non-transformed controls). Leaf extracts of transgenic lines also inhibited mycelial growth of Fon in vitro and caused hyphal abnormalities.</description><identifier>ISSN: 0032-0862</identifier><identifier>EISSN: 1365-3059</identifier><identifier>DOI: 10.1111/j.1365-3059.2010.02352.x</identifier><identifier>CODEN: PLPAAD</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Agronomy. Soil science and plant productions ; Biological and medical sciences ; Biotechnology ; ChiC gene ; Chitinase ; Chromosome rearrangements ; Colonization ; Defensins ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; Fusarium oxysporum ; Fusarium oxysporum f.sp. nicotianae ; Genetic engineering ; Genetic engineering applications ; Genetic technics ; Genetics and breeding of economic plants ; gene‐stacking ; Infection ; Leaves ; marker‐free ; MAT vector ; Methods. Procedures. Technologies ; Mycelia ; Nicotiana tabacum ; Phytopathology. Animal pests. Plant and forest protection ; Plant breeding: fundamental aspects and methodology ; Plant protection ; retransformation ; Roots ; Shoots ; Streptomyces griseus ; Survival ; Tobacco ; Transformation ; Transgenic animals and transgenic plants ; Transgenic plants ; Wilt</subject><ispartof>Plant pathology, 2011-04, Vol.60 (2), p.221-231</ispartof><rights>2010 The Authors. Plant Pathology © 2010 BSPP</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4932-62cca376d13e97c46c74f0cd32888906c36901261f6fd62305e524a0435224c43</citedby><cites>FETCH-LOGICAL-c4932-62cca376d13e97c46c74f0cd32888906c36901261f6fd62305e524a0435224c43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-3059.2010.02352.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-3059.2010.02352.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,1428,27905,27906,45555,45556,46390,46814</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23931858$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Ntui, V.O</creatorcontrib><creatorcontrib>Azadi, P</creatorcontrib><creatorcontrib>Thirukkumaran, G</creatorcontrib><creatorcontrib>Khan, R.S</creatorcontrib><creatorcontrib>Chin, D.P</creatorcontrib><creatorcontrib>Nakamura, I</creatorcontrib><creatorcontrib>Mii, M</creatorcontrib><title>Increased resistance to fusarium wilt in transgenic tobacco lines co-expressing chitinase and wasabi defensin genes</title><title>Plant pathology</title><description>Marker-free transgenic tobacco (Nicotiana tabacum) lines containing a chitinase (ChiC) gene isolated from Streptomyces griseus strain HUT 6037 were produced by Agrobacterium-mediated transformation. One marker-free transgenic line, TC-1, was retransformed with the wasabi defensin (WD) gene, isolated from Wasabia japonica. Of the retransformed shoots, 37% co-expressed the ChiC/WD genes, as confirmed by western and northern analyses. Southern blot analysis showed that no chromosomal rearrangement was introduced between the first and the second transformation. Transgenic lines either expressing ChiC or WD, or co-expressing both genes were challenged with Fusarium oxysporum f.sp. nicotianae (Fon). Assessment of in vitro plant survival in the presence of Fon showed that transgenic lines co-expressing both genes had significantly enhanced protection against the fungus (infection indices 0·0-1.·2) compared with corresponding isogenic lines expressing either of the genes (infection indices 2·5-9·8). Whole-plant infection indices in transgenic lines were significantly related (r = 0·93, P < 0·01) to the extent of root colonization of the host, which ranged from 2·1% to 11·3% in lines co-expressing both genes, and from 16·8% to 37·7% in lines expressing just one of the genes (compared with 86·4% in non-transformed controls). Leaf extracts of transgenic lines also inhibited mycelial growth of Fon in vitro and caused hyphal abnormalities.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>ChiC gene</subject><subject>Chitinase</subject><subject>Chromosome rearrangements</subject><subject>Colonization</subject><subject>Defensins</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>Fusarium oxysporum</subject><subject>Fusarium oxysporum f.sp. nicotianae</subject><subject>Genetic engineering</subject><subject>Genetic engineering applications</subject><subject>Genetic technics</subject><subject>Genetics and breeding of economic plants</subject><subject>gene‐stacking</subject><subject>Infection</subject><subject>Leaves</subject><subject>marker‐free</subject><subject>MAT vector</subject><subject>Methods. Procedures. Technologies</subject><subject>Mycelia</subject><subject>Nicotiana tabacum</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>Plant protection</subject><subject>retransformation</subject><subject>Roots</subject><subject>Shoots</subject><subject>Streptomyces griseus</subject><subject>Survival</subject><subject>Tobacco</subject><subject>Transformation</subject><subject>Transgenic animals and transgenic plants</subject><subject>Transgenic plants</subject><subject>Wilt</subject><issn>0032-0862</issn><issn>1365-3059</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqNUUtv1DAQthBILIXfgCWEOGXxK177wKGqgFaqRCXo2XInk8WrrLN4EnX773G6VQ-c8MWWv8fMfMMYl2It6_m8W0tt20aL1q-VqL9C6Vatjy_Y6hl4yVZCaNUIZ9Vr9oZoJ4RsvXcrRlcZCkbCjhekRFPMgHwaeT9TLGne8_s0TDxlPpWYaYs5QYXvIsDIh5SROIwNHg9VTSlvOfxOU8rVkMfc8ftI8S7xDnvMFeZVj_SWverjQPju6T5jt9--_rq4bK5_fL-6OL9uwPjarVUAUW9sJzX6DRgLG9ML6LRyznlhQVsvpLKyt31nVR0UW2WiMHV-ZcDoM_bp5Hso458ZaQr7RIDDEDOOMwVnhfatUa4yP_zD3I1zybW5IDfWOu2kXPzciQVlJCrYh0NJ-1geghRhWUbYhSXzsGQelmWEx2WEY5V-fCoQCeLQ1ywh0bNeaa-la5dGvpx4NXV8-G__cHNzvryq_v1J38cxxG2pNW5_VqYW0hsvndV_ATmIpbs</recordid><startdate>201104</startdate><enddate>201104</enddate><creator>Ntui, V.O</creator><creator>Azadi, P</creator><creator>Thirukkumaran, G</creator><creator>Khan, R.S</creator><creator>Chin, D.P</creator><creator>Nakamura, I</creator><creator>Mii, M</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Wiley Subscription Services, Inc</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7QO</scope></search><sort><creationdate>201104</creationdate><title>Increased resistance to fusarium wilt in transgenic tobacco lines co-expressing chitinase and wasabi defensin genes</title><author>Ntui, V.O ; Azadi, P ; Thirukkumaran, G ; Khan, R.S ; Chin, D.P ; Nakamura, I ; Mii, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4932-62cca376d13e97c46c74f0cd32888906c36901261f6fd62305e524a0435224c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>ChiC gene</topic><topic>Chitinase</topic><topic>Chromosome rearrangements</topic><topic>Colonization</topic><topic>Defensins</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal plant pathogens</topic><topic>Fusarium oxysporum</topic><topic>Fusarium oxysporum f.sp. nicotianae</topic><topic>Genetic engineering</topic><topic>Genetic engineering applications</topic><topic>Genetic technics</topic><topic>Genetics and breeding of economic plants</topic><topic>gene‐stacking</topic><topic>Infection</topic><topic>Leaves</topic><topic>marker‐free</topic><topic>MAT vector</topic><topic>Methods. Procedures. Technologies</topic><topic>Mycelia</topic><topic>Nicotiana tabacum</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>Plant protection</topic><topic>retransformation</topic><topic>Roots</topic><topic>Shoots</topic><topic>Streptomyces griseus</topic><topic>Survival</topic><topic>Tobacco</topic><topic>Transformation</topic><topic>Transgenic animals and transgenic plants</topic><topic>Transgenic plants</topic><topic>Wilt</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ntui, V.O</creatorcontrib><creatorcontrib>Azadi, P</creatorcontrib><creatorcontrib>Thirukkumaran, G</creatorcontrib><creatorcontrib>Khan, R.S</creatorcontrib><creatorcontrib>Chin, D.P</creatorcontrib><creatorcontrib>Nakamura, I</creatorcontrib><creatorcontrib>Mii, M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ntui, V.O</au><au>Azadi, P</au><au>Thirukkumaran, G</au><au>Khan, R.S</au><au>Chin, D.P</au><au>Nakamura, I</au><au>Mii, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased resistance to fusarium wilt in transgenic tobacco lines co-expressing chitinase and wasabi defensin genes</atitle><jtitle>Plant pathology</jtitle><date>2011-04</date><risdate>2011</risdate><volume>60</volume><issue>2</issue><spage>221</spage><epage>231</epage><pages>221-231</pages><issn>0032-0862</issn><eissn>1365-3059</eissn><coden>PLPAAD</coden><abstract>Marker-free transgenic tobacco (Nicotiana tabacum) lines containing a chitinase (ChiC) gene isolated from Streptomyces griseus strain HUT 6037 were produced by Agrobacterium-mediated transformation. One marker-free transgenic line, TC-1, was retransformed with the wasabi defensin (WD) gene, isolated from Wasabia japonica. Of the retransformed shoots, 37% co-expressed the ChiC/WD genes, as confirmed by western and northern analyses. Southern blot analysis showed that no chromosomal rearrangement was introduced between the first and the second transformation. Transgenic lines either expressing ChiC or WD, or co-expressing both genes were challenged with Fusarium oxysporum f.sp. nicotianae (Fon). Assessment of in vitro plant survival in the presence of Fon showed that transgenic lines co-expressing both genes had significantly enhanced protection against the fungus (infection indices 0·0-1.·2) compared with corresponding isogenic lines expressing either of the genes (infection indices 2·5-9·8). Whole-plant infection indices in transgenic lines were significantly related (r = 0·93, P < 0·01) to the extent of root colonization of the host, which ranged from 2·1% to 11·3% in lines co-expressing both genes, and from 16·8% to 37·7% in lines expressing just one of the genes (compared with 86·4% in non-transformed controls). Leaf extracts of transgenic lines also inhibited mycelial growth of Fon in vitro and caused hyphal abnormalities.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1365-3059.2010.02352.x</doi><tpages>11</tpages></addata></record> |
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| subjects | Agronomy. Soil science and plant productions Biological and medical sciences Biotechnology ChiC gene Chitinase Chromosome rearrangements Colonization Defensins Fundamental and applied biological sciences. Psychology Fungal plant pathogens Fusarium oxysporum Fusarium oxysporum f.sp. nicotianae Genetic engineering Genetic engineering applications Genetic technics Genetics and breeding of economic plants gene‐stacking Infection Leaves marker‐free MAT vector Methods. Procedures. Technologies Mycelia Nicotiana tabacum Phytopathology. Animal pests. Plant and forest protection Plant breeding: fundamental aspects and methodology Plant protection retransformation Roots Shoots Streptomyces griseus Survival Tobacco Transformation Transgenic animals and transgenic plants Transgenic plants Wilt |
| title | Increased resistance to fusarium wilt in transgenic tobacco lines co-expressing chitinase and wasabi defensin genes |
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