Stable Argonaute2 overexpression differentially regulates microRNA production
microRNAs are a class of small RNA molecules that associate with Argonaute proteins to regulate gene expression. Argonaute proteins not only mediate the functions of microRNAs, but also play an essential role in the biogenesis of microRNAs. Here, we report that stable, long-term overexpression of Ar...
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| Veröffentlicht in: | Biochimica et biophysica acta 2009-02, Vol.1789 (2), p.153-159 |
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| creator | Zhang, Xiaoxiao Graves, Paul R. Zeng, Yan |
| description | microRNAs are a class of small RNA molecules that associate with Argonaute proteins to regulate gene expression. Argonaute proteins not only mediate the functions of microRNAs, but also play an essential role in the biogenesis of microRNAs. Here, we report that stable, long-term overexpression of Argonaute2, an Argonaute isoform, induces the production of a number of microRNAs, such as the let-7 family of microRNAs, in 293T cells. On the other hand, the expression of many microRNAs is insensitive to elevated Argonaute levels, and microRNAs in the miR-17-92 and homologous clusters are even down-regulated. The down-regulation may result from the let-7-mediated inhibition of the expression of Myc, which positively controls the transcription of the microRNAs clusters. Our data suggest that human cells have a mechanism for microRNA homeostasis, and that overexpression of a general microRNA processing factor can differentially regulate the expression of specific microRNAs. |
| doi_str_mv | 10.1016/j.bbagrm.2008.11.004 |
| format | Article |
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Argonaute proteins not only mediate the functions of microRNAs, but also play an essential role in the biogenesis of microRNAs. Here, we report that stable, long-term overexpression of Argonaute2, an Argonaute isoform, induces the production of a number of microRNAs, such as the let-7 family of microRNAs, in 293T cells. On the other hand, the expression of many microRNAs is insensitive to elevated Argonaute levels, and microRNAs in the miR-17-92 and homologous clusters are even down-regulated. The down-regulation may result from the let-7-mediated inhibition of the expression of Myc, which positively controls the transcription of the microRNAs clusters. 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Argonaute proteins not only mediate the functions of microRNAs, but also play an essential role in the biogenesis of microRNAs. Here, we report that stable, long-term overexpression of Argonaute2, an Argonaute isoform, induces the production of a number of microRNAs, such as the let-7 family of microRNAs, in 293T cells. On the other hand, the expression of many microRNAs is insensitive to elevated Argonaute levels, and microRNAs in the miR-17-92 and homologous clusters are even down-regulated. The down-regulation may result from the let-7-mediated inhibition of the expression of Myc, which positively controls the transcription of the microRNAs clusters. Our data suggest that human cells have a mechanism for microRNA homeostasis, and that overexpression of a general microRNA processing factor can differentially regulate the expression of specific microRNAs.</description><subject>Argonaute Proteins</subject><subject>Argonaute2 (Ago2)</subject><subject>Blotting, Northern</subject><subject>Cell Line</subject><subject>Eukaryotic Initiation Factor-2 - genetics</subject><subject>Eukaryotic Initiation Factor-2 - physiology</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>let-7</subject><subject>microRNA (miRNA)</subject><subject>MicroRNAs - biosynthesis</subject><subject>miR-17-92</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Polymerase Chain Reaction</subject><subject>RNA Interference - physiology</subject><issn>1874-9399</issn><issn>0006-3002</issn><issn>1876-4320</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotlb_gcie9LRrstnNbi5CKX5BVfDjHLLJpKTsR012i_33prbgracZhmfeYR6ELglOCCbsdplUlVy4JkkxLhNCEoyzIzQmZcHijKb4-K_PYk45H6Ez75cYMxLgUzQiHLMM43yMXj56WdUQTd2ia-XQQxp1a3Dws3Lgve3aSFtjwqDtrazrTeRgMdSyBx81Vrnu_XUarVynB9UH-BydGFl7uNjXCfp6uP-cPcXzt8fn2XQeK8rTPgZZ8pLorOCqYMrkpjKSVTrXqlC5lKTASvJSU2loYQjRtEyVLljKlVEZSXM6QTe73HD6ewDfi8Z6BXUtW-gGL0qGKc84xYG8PkgyVqYFpdvIbAeGp7x3YMTK2Ua6jSBYbIWLpdgJF1vhghARhIe1q33-UDWg_5f2hgNwtwMg-FhbcMIrC60CbR2oXujOHr7wCw4glOs</recordid><startdate>20090201</startdate><enddate>20090201</enddate><creator>Zhang, Xiaoxiao</creator><creator>Graves, Paul R.</creator><creator>Zeng, Yan</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20090201</creationdate><title>Stable Argonaute2 overexpression differentially regulates microRNA production</title><author>Zhang, Xiaoxiao ; Graves, Paul R. ; Zeng, Yan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-ea8981d479c76cf5fbfa6bd5dc7c5aa170ca98d3af37f11d382cd7629cfc41253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Argonaute Proteins</topic><topic>Argonaute2 (Ago2)</topic><topic>Blotting, Northern</topic><topic>Cell Line</topic><topic>Eukaryotic Initiation Factor-2 - genetics</topic><topic>Eukaryotic Initiation Factor-2 - physiology</topic><topic>Gene Expression Regulation</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>let-7</topic><topic>microRNA (miRNA)</topic><topic>MicroRNAs - biosynthesis</topic><topic>miR-17-92</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Polymerase Chain Reaction</topic><topic>RNA Interference - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Xiaoxiao</creatorcontrib><creatorcontrib>Graves, Paul R.</creatorcontrib><creatorcontrib>Zeng, Yan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Biochimica et biophysica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Xiaoxiao</au><au>Graves, Paul R.</au><au>Zeng, Yan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stable Argonaute2 overexpression differentially regulates microRNA production</atitle><jtitle>Biochimica et biophysica acta</jtitle><addtitle>Biochim Biophys Acta</addtitle><date>2009-02-01</date><risdate>2009</risdate><volume>1789</volume><issue>2</issue><spage>153</spage><epage>159</epage><pages>153-159</pages><issn>1874-9399</issn><issn>0006-3002</issn><eissn>1876-4320</eissn><abstract>microRNAs are a class of small RNA molecules that associate with Argonaute proteins to regulate gene expression. Argonaute proteins not only mediate the functions of microRNAs, but also play an essential role in the biogenesis of microRNAs. Here, we report that stable, long-term overexpression of Argonaute2, an Argonaute isoform, induces the production of a number of microRNAs, such as the let-7 family of microRNAs, in 293T cells. On the other hand, the expression of many microRNAs is insensitive to elevated Argonaute levels, and microRNAs in the miR-17-92 and homologous clusters are even down-regulated. The down-regulation may result from the let-7-mediated inhibition of the expression of Myc, which positively controls the transcription of the microRNAs clusters. Our data suggest that human cells have a mechanism for microRNA homeostasis, and that overexpression of a general microRNA processing factor can differentially regulate the expression of specific microRNAs.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>19064005</pmid><doi>10.1016/j.bbagrm.2008.11.004</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochimica et biophysica acta, 2009-02, Vol.1789 (2), p.153-159 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Argonaute Proteins Argonaute2 (Ago2) Blotting, Northern Cell Line Eukaryotic Initiation Factor-2 - genetics Eukaryotic Initiation Factor-2 - physiology Gene Expression Regulation Humans Immunoblotting let-7 microRNA (miRNA) MicroRNAs - biosynthesis miR-17-92 Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction RNA Interference - physiology |
| title | Stable Argonaute2 overexpression differentially regulates microRNA production |
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