Production of cyclodextrins in a fluidized-bed reactor using cyclodextrin-glycosyl-transferase

Cyclodextrin-glycosyl-transferase (EC2.4.1.19), produced by Wacker (Munich, Germany), was purified by biospecific affinity chromatography with beta-cyclodextrin (beta-CD) as ligand, and immobilized into controlled pore silica particles (0.42 mm). This immobilized enzyme (IE) had 4.7 mg of protein/g...

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Veröffentlicht in:	Applied biochemistry and biotechnology 2000, Vol.84 (1-9), p.1003-1019
Hauptverfasser:	Tardioli, P.W, Zanin, G.M, Moraes, F.F. de
Format:	Artikel
Sprache:	eng
Schlagworte:	affinity chromatography Batch reactors beta-cyclodextrin Bioconversions. Hemisynthesis Biological and medical sciences Bioreactors Biotechnology Biotechnology - instrumentation Biotechnology - methods Chromatography Chromatography, Affinity Cyclodextrins - biosynthesis Cyclodextrins - chemical synthesis Enzymes Enzymes, Immobilized - metabolism equations Fluidization fluidized beds Fundamental and applied biological sciences. Psychology Glucosyltransferases - isolation & purification Glucosyltransferases - metabolism immobilized enzymes Kinetics maltodextrins Methods. Procedures. Technologies Reactors Sepharose Silica Substrates
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