Sister chromatid exchange (SCE) and high-frequency cells (HFC) in peripheral blood lymphocytes of healthy Tunisian smokers
Cigarette smoking is a major public health problem in Tunisia as it concerns up to 30–35% of the adult population, raising important national issues on tobacco-related disease. The aim of this study was to establish whether cigarette smoking increases sister chromatid exchange (SCE) in peripheral bl...
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| Veröffentlicht in: | Mutation research 2011-02, Vol.719 (1-2), p.1-6 |
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| creator | Ben Salah, Ghada Kamoun, Hassen Rebai, Ahmed Ben Youssef, Achraf Ayadi, Hajer Belghith-Mahfoudh, Neila Fourati, Amine Ayadi, Hamadi Fakhfakh, Faiza |
| description | Cigarette smoking is a major public health problem in Tunisia as it concerns up to 30–35% of the adult population, raising important national issues on tobacco-related disease. The aim of this study was to establish whether cigarette smoking increases sister chromatid exchange (SCE) in peripheral blood lymphocytes of smokers (n=14) compared with non-smokers (n=15) in Sfax, Tunisia. The smokers were subdivided in two subgroups according to the duration of the smoking habit: heavy smokers (>10 years) and light smokers (≤10 years). After signing a consent form, volunteers provided a blood sample (5ml) to establish cell cultures during 72h. For SCE analysis, 30 second-division metaphases were examined from each subject. We determined the frequency of SCE, the percentage of high-frequency cells (HFC) and that of the high-frequency cell individual (HFI). The results show a significantly higher SCE frequency in smokers (8.65±1.43) than in non-smokers (7.16±1.3; p |
| doi_str_mv | 10.1016/j.mrgentox.2010.09.003 |
| format | Article |
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The aim of this study was to establish whether cigarette smoking increases sister chromatid exchange (SCE) in peripheral blood lymphocytes of smokers (n=14) compared with non-smokers (n=15) in Sfax, Tunisia. The smokers were subdivided in two subgroups according to the duration of the smoking habit: heavy smokers (>10 years) and light smokers (≤10 years). After signing a consent form, volunteers provided a blood sample (5ml) to establish cell cultures during 72h. For SCE analysis, 30 second-division metaphases were examined from each subject. We determined the frequency of SCE, the percentage of high-frequency cells (HFC) and that of the high-frequency cell individual (HFI). The results show a significantly higher SCE frequency in smokers (8.65±1.43) than in non-smokers (7.16±1.3; p<0.01). A significant difference in SCE frequency was also shown when comparing the two subgroups of smokers (p<0.05). Interestingly, no significant difference was found when comparing the light smokers with non-smokers (7.82±1 vs 7.16±1.3, respectively, p>0.05). The percentages of HFC and HFI were significantly higher in smokers (11.2±7.8% and 78.6%, respectively) than in non-smokers (4±2.2% and 20%, respectively, p<0.01). Our study indicates that the genotoxic effects in lymphocytes from healthy Tunisian smokers are most likely caused by cigarette-smoke constituents. This effect was mainly observed in smokers who had been smoking during more than 10 years. These results provide scientific evidence to urge the prevention of tobacco consumption.</description><identifier>ISSN: 1383-5718</identifier><identifier>ISSN: 0027-5107</identifier><identifier>EISSN: 1879-3592</identifier><identifier>DOI: 10.1016/j.mrgentox.2010.09.003</identifier><identifier>PMID: 20869462</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adult ; Adults ; Biological and medical sciences ; Cell culture ; Cells, Cultured ; Cigarettes ; Comparative analysis ; Cytogenetic Analysis - statistics & numerical data ; DNA damage ; Fundamental and applied biological sciences. Psychology ; Genetics of eukaryotes. Biological and molecular evolution ; High-frequency cell ; High-frequency cells individual ; Humans ; Lymphocytes ; Lymphocytes - cytology ; Lymphocytes - metabolism ; Male ; Medical sciences ; Metaphase - genetics ; Middle Aged ; Public health ; Sister chromatid exchange ; Sister Chromatid Exchange - genetics ; Smoking ; Smoking cessation ; Studies ; Time Factors ; Tobacco, tobacco smoking ; Toxicology ; Tunisia ; Tunisian population ; Young Adult</subject><ispartof>Mutation research, 2011-02, Vol.719 (1-2), p.1-6</ispartof><rights>2010 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><rights>Copyright Elsevier BV Feb 3, 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-2fd64f89af409bf2b4d679fc59ecdb7f2473845f08ccb3d20fde7f345b36f5b53</citedby><cites>FETCH-LOGICAL-c456t-2fd64f89af409bf2b4d679fc59ecdb7f2473845f08ccb3d20fde7f345b36f5b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mrgentox.2010.09.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23753347$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20869462$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ben Salah, Ghada</creatorcontrib><creatorcontrib>Kamoun, Hassen</creatorcontrib><creatorcontrib>Rebai, Ahmed</creatorcontrib><creatorcontrib>Ben Youssef, Achraf</creatorcontrib><creatorcontrib>Ayadi, Hajer</creatorcontrib><creatorcontrib>Belghith-Mahfoudh, Neila</creatorcontrib><creatorcontrib>Fourati, Amine</creatorcontrib><creatorcontrib>Ayadi, Hamadi</creatorcontrib><creatorcontrib>Fakhfakh, Faiza</creatorcontrib><title>Sister chromatid exchange (SCE) and high-frequency cells (HFC) in peripheral blood lymphocytes of healthy Tunisian smokers</title><title>Mutation research</title><addtitle>Mutat Res</addtitle><description>Cigarette smoking is a major public health problem in Tunisia as it concerns up to 30–35% of the adult population, raising important national issues on tobacco-related disease. The aim of this study was to establish whether cigarette smoking increases sister chromatid exchange (SCE) in peripheral blood lymphocytes of smokers (n=14) compared with non-smokers (n=15) in Sfax, Tunisia. The smokers were subdivided in two subgroups according to the duration of the smoking habit: heavy smokers (>10 years) and light smokers (≤10 years). After signing a consent form, volunteers provided a blood sample (5ml) to establish cell cultures during 72h. For SCE analysis, 30 second-division metaphases were examined from each subject. We determined the frequency of SCE, the percentage of high-frequency cells (HFC) and that of the high-frequency cell individual (HFI). The results show a significantly higher SCE frequency in smokers (8.65±1.43) than in non-smokers (7.16±1.3; p<0.01). A significant difference in SCE frequency was also shown when comparing the two subgroups of smokers (p<0.05). Interestingly, no significant difference was found when comparing the light smokers with non-smokers (7.82±1 vs 7.16±1.3, respectively, p>0.05). The percentages of HFC and HFI were significantly higher in smokers (11.2±7.8% and 78.6%, respectively) than in non-smokers (4±2.2% and 20%, respectively, p<0.01). Our study indicates that the genotoxic effects in lymphocytes from healthy Tunisian smokers are most likely caused by cigarette-smoke constituents. This effect was mainly observed in smokers who had been smoking during more than 10 years. 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Psychology</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>High-frequency cell</topic><topic>High-frequency cells individual</topic><topic>Humans</topic><topic>Lymphocytes</topic><topic>Lymphocytes - cytology</topic><topic>Lymphocytes - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Metaphase - genetics</topic><topic>Middle Aged</topic><topic>Public health</topic><topic>Sister chromatid exchange</topic><topic>Sister Chromatid Exchange - genetics</topic><topic>Smoking</topic><topic>Smoking cessation</topic><topic>Studies</topic><topic>Time Factors</topic><topic>Tobacco, tobacco smoking</topic><topic>Toxicology</topic><topic>Tunisia</topic><topic>Tunisian population</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ben Salah, Ghada</creatorcontrib><creatorcontrib>Kamoun, Hassen</creatorcontrib><creatorcontrib>Rebai, Ahmed</creatorcontrib><creatorcontrib>Ben Youssef, Achraf</creatorcontrib><creatorcontrib>Ayadi, Hajer</creatorcontrib><creatorcontrib>Belghith-Mahfoudh, Neila</creatorcontrib><creatorcontrib>Fourati, Amine</creatorcontrib><creatorcontrib>Ayadi, Hamadi</creatorcontrib><creatorcontrib>Fakhfakh, Faiza</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>Pollution Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Mutation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ben Salah, Ghada</au><au>Kamoun, Hassen</au><au>Rebai, Ahmed</au><au>Ben Youssef, Achraf</au><au>Ayadi, Hajer</au><au>Belghith-Mahfoudh, Neila</au><au>Fourati, Amine</au><au>Ayadi, Hamadi</au><au>Fakhfakh, Faiza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sister chromatid exchange (SCE) and high-frequency cells (HFC) in peripheral blood lymphocytes of healthy Tunisian smokers</atitle><jtitle>Mutation research</jtitle><addtitle>Mutat Res</addtitle><date>2011-02-03</date><risdate>2011</risdate><volume>719</volume><issue>1-2</issue><spage>1</spage><epage>6</epage><pages>1-6</pages><issn>1383-5718</issn><issn>0027-5107</issn><eissn>1879-3592</eissn><abstract>Cigarette smoking is a major public health problem in Tunisia as it concerns up to 30–35% of the adult population, raising important national issues on tobacco-related disease. The aim of this study was to establish whether cigarette smoking increases sister chromatid exchange (SCE) in peripheral blood lymphocytes of smokers (n=14) compared with non-smokers (n=15) in Sfax, Tunisia. The smokers were subdivided in two subgroups according to the duration of the smoking habit: heavy smokers (>10 years) and light smokers (≤10 years). After signing a consent form, volunteers provided a blood sample (5ml) to establish cell cultures during 72h. For SCE analysis, 30 second-division metaphases were examined from each subject. We determined the frequency of SCE, the percentage of high-frequency cells (HFC) and that of the high-frequency cell individual (HFI). The results show a significantly higher SCE frequency in smokers (8.65±1.43) than in non-smokers (7.16±1.3; p<0.01). A significant difference in SCE frequency was also shown when comparing the two subgroups of smokers (p<0.05). Interestingly, no significant difference was found when comparing the light smokers with non-smokers (7.82±1 vs 7.16±1.3, respectively, p>0.05). The percentages of HFC and HFI were significantly higher in smokers (11.2±7.8% and 78.6%, respectively) than in non-smokers (4±2.2% and 20%, respectively, p<0.01). Our study indicates that the genotoxic effects in lymphocytes from healthy Tunisian smokers are most likely caused by cigarette-smoke constituents. This effect was mainly observed in smokers who had been smoking during more than 10 years. These results provide scientific evidence to urge the prevention of tobacco consumption.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>20869462</pmid><doi>10.1016/j.mrgentox.2010.09.003</doi><tpages>6</tpages></addata></record> |
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| subjects | Adult Adults Biological and medical sciences Cell culture Cells, Cultured Cigarettes Comparative analysis Cytogenetic Analysis - statistics & numerical data DNA damage Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution High-frequency cell High-frequency cells individual Humans Lymphocytes Lymphocytes - cytology Lymphocytes - metabolism Male Medical sciences Metaphase - genetics Middle Aged Public health Sister chromatid exchange Sister Chromatid Exchange - genetics Smoking Smoking cessation Studies Time Factors Tobacco, tobacco smoking Toxicology Tunisia Tunisian population Young Adult |
| title | Sister chromatid exchange (SCE) and high-frequency cells (HFC) in peripheral blood lymphocytes of healthy Tunisian smokers |
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