Double-stranded RNA confers both preventive and therapeutic effects against Penaeus stylirostris densovirus ( PstDNV) in Litopenaeus vannamei
Penaeus stylirostris densovirus ( PstDNV) infection is found widespread in peneaid shrimp, especially in economically important species such as black tiger shrimp Penaeus monodon and Pacific white shrimp Litopenaeus vannamei. Although effective prevention method for viral diseases is not well establ...
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| Veröffentlicht in: | Virus research 2011, Vol.155 (1), p.131-136 |
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| creator | Ho, Teerapong Yasri, Pratchayapong Panyim, Sakol Udomkit, Apinunt |
| description | Penaeus stylirostris densovirus (
PstDNV) infection is found widespread in peneaid shrimp, especially in economically important species such as black tiger shrimp
Penaeus monodon and Pacific white shrimp
Litopenaeus vannamei. Although effective prevention method for viral diseases is not well established in shrimp, the treatment with viral specific double-stranded RNA (dsRNA) or siRNA has given promising results. In present study, dsRNAs corresponding to non-structural (ORF1 and ORF2 overlapping sequence) and structural (ORF3) genes of
PstDNV were investigated for their potency to inhibit
PstDNV replication in the shrimp. Periodically injection of either ORF1-2 dsRNA or ORF3 dsRNA at three days interval into
L. vannamei resulted in substantial inhibition of
PstDNV infection. In addition, a possibility for a therapeutic application of dsRNA in
PstDNV-infected shrimp was demonstrated by the efficient suppression of
PstDNV replication in
L. vannamei when the ORF1-2 dsRNA was delivered into the shrimp within 24
h post-
PstDNV injection. Hence, our results established both the preventive and therapeutic potency of dsRNA to inhibit
PstDNV in
L. vannamei that could be applied as a potential treatment of
PstDNV infection in shrimp. |
| doi_str_mv | 10.1016/j.virusres.2010.09.009 |
| format | Article |
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PstDNV) infection is found widespread in peneaid shrimp, especially in economically important species such as black tiger shrimp
Penaeus monodon and Pacific white shrimp
Litopenaeus vannamei. Although effective prevention method for viral diseases is not well established in shrimp, the treatment with viral specific double-stranded RNA (dsRNA) or siRNA has given promising results. In present study, dsRNAs corresponding to non-structural (ORF1 and ORF2 overlapping sequence) and structural (ORF3) genes of
PstDNV were investigated for their potency to inhibit
PstDNV replication in the shrimp. Periodically injection of either ORF1-2 dsRNA or ORF3 dsRNA at three days interval into
L. vannamei resulted in substantial inhibition of
PstDNV infection. In addition, a possibility for a therapeutic application of dsRNA in
PstDNV-infected shrimp was demonstrated by the efficient suppression of
PstDNV replication in
L. vannamei when the ORF1-2 dsRNA was delivered into the shrimp within 24
h post-
PstDNV injection. Hence, our results established both the preventive and therapeutic potency of dsRNA to inhibit
PstDNV in
L. vannamei that could be applied as a potential treatment of
PstDNV infection in shrimp.</description><identifier>ISSN: 0168-1702</identifier><identifier>EISSN: 1872-7492</identifier><identifier>DOI: 10.1016/j.virusres.2010.09.009</identifier><identifier>PMID: 20869997</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antiviral Agents - administration & dosage ; Antiviral Agents - pharmacology ; antiviral properties ; Densovirus ; Densovirus - drug effects ; Densovirus - growth & development ; double-stranded RNA ; genes ; injection ; Litopenaeus stylirostris ; Litopenaeus vannamei ; Parvoviruses ; Penaeidae - virology ; Penaeus monodon ; Penaeus stylirostris ; RNA interference ; RNA, Double-Stranded - administration & dosage ; RNA, Double-Stranded - pharmacology ; RNA, Viral - administration & dosage ; RNA, Viral - pharmacology ; Runt-deformity syndrome ; Shrimp ; small interfering RNA ; therapeutics ; viral diseases of animals and humans ; virus replication ; Virus Replication - drug effects ; viruses</subject><ispartof>Virus research, 2011, Vol.155 (1), p.131-136</ispartof><rights>2010 Elsevier B.V.</rights><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-47a377ca6351fd46a0de2a9a1cd1b56da70c7b363ece38e3b7e0859db860f5aa3</citedby><cites>FETCH-LOGICAL-c423t-47a377ca6351fd46a0de2a9a1cd1b56da70c7b363ece38e3b7e0859db860f5aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.virusres.2010.09.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,4009,27902,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20869997$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ho, Teerapong</creatorcontrib><creatorcontrib>Yasri, Pratchayapong</creatorcontrib><creatorcontrib>Panyim, Sakol</creatorcontrib><creatorcontrib>Udomkit, Apinunt</creatorcontrib><title>Double-stranded RNA confers both preventive and therapeutic effects against Penaeus stylirostris densovirus ( PstDNV) in Litopenaeus vannamei</title><title>Virus research</title><addtitle>Virus Res</addtitle><description>Penaeus stylirostris densovirus (
PstDNV) infection is found widespread in peneaid shrimp, especially in economically important species such as black tiger shrimp
Penaeus monodon and Pacific white shrimp
Litopenaeus vannamei. Although effective prevention method for viral diseases is not well established in shrimp, the treatment with viral specific double-stranded RNA (dsRNA) or siRNA has given promising results. In present study, dsRNAs corresponding to non-structural (ORF1 and ORF2 overlapping sequence) and structural (ORF3) genes of
PstDNV were investigated for their potency to inhibit
PstDNV replication in the shrimp. Periodically injection of either ORF1-2 dsRNA or ORF3 dsRNA at three days interval into
L. vannamei resulted in substantial inhibition of
PstDNV infection. In addition, a possibility for a therapeutic application of dsRNA in
PstDNV-infected shrimp was demonstrated by the efficient suppression of
PstDNV replication in
L. vannamei when the ORF1-2 dsRNA was delivered into the shrimp within 24
h post-
PstDNV injection. Hence, our results established both the preventive and therapeutic potency of dsRNA to inhibit
PstDNV in
L. vannamei that could be applied as a potential treatment of
PstDNV infection in shrimp.</description><subject>Animals</subject><subject>Antiviral Agents - administration & dosage</subject><subject>Antiviral Agents - pharmacology</subject><subject>antiviral properties</subject><subject>Densovirus</subject><subject>Densovirus - drug effects</subject><subject>Densovirus - growth & development</subject><subject>double-stranded RNA</subject><subject>genes</subject><subject>injection</subject><subject>Litopenaeus stylirostris</subject><subject>Litopenaeus vannamei</subject><subject>Parvoviruses</subject><subject>Penaeidae - virology</subject><subject>Penaeus monodon</subject><subject>Penaeus stylirostris</subject><subject>RNA interference</subject><subject>RNA, Double-Stranded - administration & dosage</subject><subject>RNA, Double-Stranded - pharmacology</subject><subject>RNA, Viral - administration & dosage</subject><subject>RNA, Viral - pharmacology</subject><subject>Runt-deformity syndrome</subject><subject>Shrimp</subject><subject>small interfering RNA</subject><subject>therapeutics</subject><subject>viral diseases of animals and humans</subject><subject>virus replication</subject><subject>Virus Replication - drug effects</subject><subject>viruses</subject><issn>0168-1702</issn><issn>1872-7492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1DAUhiMEokPhFYp3wCLDcS52vKNquUmjUgFlazn2SetRxg62E6kPwTvjYaZsu7Jkff_v4_MVxRmFNQXK3m_Xiw1zDBjXFeRLEGsA8aRY0Y5XJW9E9bRYZbArKYfqpHgR4xYAWM3Z8-Kkgo4JIfiq-HPp537EMqagnEFDvl-dE-3dgCGS3qc7MgVc0CW7IMkESXcY1IRzsprgMKBOkahbZV1M5BqdwjmSmO5HG3zutJEYdNH_G5a8JdcxXV79ekesIxub_HQMLMo5tUP7sng2qDHiq-N5Wtx8-vjz4ku5-fb568X5ptRNVaey4armXCtWt3QwDVNgsFJCUW1o3zKjOGje16xGjXWHdc8RulaYvmMwtErVp8WbQ-8U_O8ZY5I7GzWOo3Lo5ygzV3cttPRxsqJNJwTbk-xA6vzzLGaQU7A7Fe4lBbl3JrfywZncO5MgZHaWg2fHJ-Z-h-Z_7EFSBl4fgEF5qW7zUuXNj9zQAtCOtgIy8eFAYF7aYjHIqC06jcaGbEgabx-b4i8WubiL</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>Ho, Teerapong</creator><creator>Yasri, Pratchayapong</creator><creator>Panyim, Sakol</creator><creator>Udomkit, Apinunt</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>7U9</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope></search><sort><creationdate>2011</creationdate><title>Double-stranded RNA confers both preventive and therapeutic effects against Penaeus stylirostris densovirus ( PstDNV) in Litopenaeus vannamei</title><author>Ho, Teerapong ; Yasri, Pratchayapong ; Panyim, Sakol ; Udomkit, Apinunt</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-47a377ca6351fd46a0de2a9a1cd1b56da70c7b363ece38e3b7e0859db860f5aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Antiviral Agents - administration & dosage</topic><topic>Antiviral Agents - pharmacology</topic><topic>antiviral properties</topic><topic>Densovirus</topic><topic>Densovirus - drug effects</topic><topic>Densovirus - growth & development</topic><topic>double-stranded RNA</topic><topic>genes</topic><topic>injection</topic><topic>Litopenaeus stylirostris</topic><topic>Litopenaeus vannamei</topic><topic>Parvoviruses</topic><topic>Penaeidae - virology</topic><topic>Penaeus monodon</topic><topic>Penaeus stylirostris</topic><topic>RNA interference</topic><topic>RNA, Double-Stranded - administration & dosage</topic><topic>RNA, Double-Stranded - pharmacology</topic><topic>RNA, Viral - administration & dosage</topic><topic>RNA, Viral - pharmacology</topic><topic>Runt-deformity syndrome</topic><topic>Shrimp</topic><topic>small interfering RNA</topic><topic>therapeutics</topic><topic>viral diseases of animals and humans</topic><topic>virus replication</topic><topic>Virus Replication - drug effects</topic><topic>viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ho, Teerapong</creatorcontrib><creatorcontrib>Yasri, Pratchayapong</creatorcontrib><creatorcontrib>Panyim, Sakol</creatorcontrib><creatorcontrib>Udomkit, Apinunt</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Virus research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ho, Teerapong</au><au>Yasri, Pratchayapong</au><au>Panyim, Sakol</au><au>Udomkit, Apinunt</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Double-stranded RNA confers both preventive and therapeutic effects against Penaeus stylirostris densovirus ( PstDNV) in Litopenaeus vannamei</atitle><jtitle>Virus research</jtitle><addtitle>Virus Res</addtitle><date>2011</date><risdate>2011</risdate><volume>155</volume><issue>1</issue><spage>131</spage><epage>136</epage><pages>131-136</pages><issn>0168-1702</issn><eissn>1872-7492</eissn><abstract>Penaeus stylirostris densovirus (
PstDNV) infection is found widespread in peneaid shrimp, especially in economically important species such as black tiger shrimp
Penaeus monodon and Pacific white shrimp
Litopenaeus vannamei. Although effective prevention method for viral diseases is not well established in shrimp, the treatment with viral specific double-stranded RNA (dsRNA) or siRNA has given promising results. In present study, dsRNAs corresponding to non-structural (ORF1 and ORF2 overlapping sequence) and structural (ORF3) genes of
PstDNV were investigated for their potency to inhibit
PstDNV replication in the shrimp. Periodically injection of either ORF1-2 dsRNA or ORF3 dsRNA at three days interval into
L. vannamei resulted in substantial inhibition of
PstDNV infection. In addition, a possibility for a therapeutic application of dsRNA in
PstDNV-infected shrimp was demonstrated by the efficient suppression of
PstDNV replication in
L. vannamei when the ORF1-2 dsRNA was delivered into the shrimp within 24
h post-
PstDNV injection. Hence, our results established both the preventive and therapeutic potency of dsRNA to inhibit
PstDNV in
L. vannamei that could be applied as a potential treatment of
PstDNV infection in shrimp.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>20869997</pmid><doi>10.1016/j.virusres.2010.09.009</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-1702 |
| ispartof | Virus research, 2011, Vol.155 (1), p.131-136 |
| issn | 0168-1702 1872-7492 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_860385051 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antiviral Agents - administration & dosage Antiviral Agents - pharmacology antiviral properties Densovirus Densovirus - drug effects Densovirus - growth & development double-stranded RNA genes injection Litopenaeus stylirostris Litopenaeus vannamei Parvoviruses Penaeidae - virology Penaeus monodon Penaeus stylirostris RNA interference RNA, Double-Stranded - administration & dosage RNA, Double-Stranded - pharmacology RNA, Viral - administration & dosage RNA, Viral - pharmacology Runt-deformity syndrome Shrimp small interfering RNA therapeutics viral diseases of animals and humans virus replication Virus Replication - drug effects viruses |
| title | Double-stranded RNA confers both preventive and therapeutic effects against Penaeus stylirostris densovirus ( PstDNV) in Litopenaeus vannamei |
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