Differential localization of neuregulin-1 type III in the central and peripheral nervous system
Abstract In the developing PNS, axonal neuregulin-1 (NRG1) type III is the key determinant for myelination. However, the specific role for NRG1 (III) in the CNS has not been established. To address this issue, isotype-specific antibodies were generated, characterized, and used for the immunofluoresc...
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| Veröffentlicht in: | Brain research 2011-01, Vol.1369 (19), p.10-20 |
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| creator | Bare, Dan J Becker-Catania, Sara G DeVries, George H |
| description | Abstract In the developing PNS, axonal neuregulin-1 (NRG1) type III is the key determinant for myelination. However, the specific role for NRG1 (III) in the CNS has not been established. To address this issue, isotype-specific antibodies were generated, characterized, and used for the immunofluorescent localization of NRG1 (III) in the developing and adult CNS of rat. In contrast to adult peripheral nerve, which showed robust axonal staining, no immunoreactivity was observed in CNS myelinated tracts during the period of active myelination or in the adult CNS. Surprisingly, NRG1 (III) was prominently expressed on dendrites and soma in both the developing and adult CNS. These findings were corroborated through the subcellular fractionation of adult rat brain combined with an immunoblotting analysis. The immunolocalization of NRG1 (III) suggests that it plays a novel role in the myelination fate of CNS axons possibly through undetermined roles in neuronal maturation, or dendritic development and activation. |
| doi_str_mv | 10.1016/j.brainres.2010.10.092 |
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However, the specific role for NRG1 (III) in the CNS has not been established. To address this issue, isotype-specific antibodies were generated, characterized, and used for the immunofluorescent localization of NRG1 (III) in the developing and adult CNS of rat. In contrast to adult peripheral nerve, which showed robust axonal staining, no immunoreactivity was observed in CNS myelinated tracts during the period of active myelination or in the adult CNS. Surprisingly, NRG1 (III) was prominently expressed on dendrites and soma in both the developing and adult CNS. These findings were corroborated through the subcellular fractionation of adult rat brain combined with an immunoblotting analysis. The immunolocalization of NRG1 (III) suggests that it plays a novel role in the myelination fate of CNS axons possibly through undetermined roles in neuronal maturation, or dendritic development and activation.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/j.brainres.2010.10.092</identifier><identifier>PMID: 21044615</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>adults ; Animals ; antibodies ; Antibody Specificity ; axons ; Biological and medical sciences ; brain ; Central Nervous System - metabolism ; Dendrite ; dendrites ; erbB ; Female ; Fluorescent Antibody Technique ; fractionation ; Fundamental and applied biological sciences. Psychology ; General aspects. Models. Methods ; Immunoblotting ; Immunolocalization ; Immunoprecipitation ; Male ; Mice ; Myelination ; nerve tissue ; Neuregulin-1 - analysis ; Neuregulin-1 - biosynthesis ; Neurogenesis - physiology ; Neurology ; Neurons - metabolism ; peripheral nervous system ; Peripheral Nervous System - metabolism ; Protein Isoforms - analysis ; Protein Isoforms - biosynthesis ; Rats ; Rats, Sprague-Dawley ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research, 2011-01, Vol.1369 (19), p.10-20</ispartof><rights>2010</rights><rights>2015 INIST-CNRS</rights><rights>Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c508t-865833113d1b5a1dafe17600d693d1c408345a2894651b17d9d0ec4551200653</citedby><cites>FETCH-LOGICAL-c508t-865833113d1b5a1dafe17600d693d1c408345a2894651b17d9d0ec4551200653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.brainres.2010.10.092$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23753250$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21044615$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bare, Dan J</creatorcontrib><creatorcontrib>Becker-Catania, Sara G</creatorcontrib><creatorcontrib>DeVries, George H</creatorcontrib><title>Differential localization of neuregulin-1 type III in the central and peripheral nervous system</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>Abstract In the developing PNS, axonal neuregulin-1 (NRG1) type III is the key determinant for myelination. However, the specific role for NRG1 (III) in the CNS has not been established. To address this issue, isotype-specific antibodies were generated, characterized, and used for the immunofluorescent localization of NRG1 (III) in the developing and adult CNS of rat. In contrast to adult peripheral nerve, which showed robust axonal staining, no immunoreactivity was observed in CNS myelinated tracts during the period of active myelination or in the adult CNS. Surprisingly, NRG1 (III) was prominently expressed on dendrites and soma in both the developing and adult CNS. These findings were corroborated through the subcellular fractionation of adult rat brain combined with an immunoblotting analysis. The immunolocalization of NRG1 (III) suggests that it plays a novel role in the myelination fate of CNS axons possibly through undetermined roles in neuronal maturation, or dendritic development and activation.</description><subject>adults</subject><subject>Animals</subject><subject>antibodies</subject><subject>Antibody Specificity</subject><subject>axons</subject><subject>Biological and medical sciences</subject><subject>brain</subject><subject>Central Nervous System - metabolism</subject><subject>Dendrite</subject><subject>dendrites</subject><subject>erbB</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>fractionation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects. Models. Methods</subject><subject>Immunoblotting</subject><subject>Immunolocalization</subject><subject>Immunoprecipitation</subject><subject>Male</subject><subject>Mice</subject><subject>Myelination</subject><subject>nerve tissue</subject><subject>Neuregulin-1 - analysis</subject><subject>Neuregulin-1 - biosynthesis</subject><subject>Neurogenesis - physiology</subject><subject>Neurology</subject><subject>Neurons - metabolism</subject><subject>peripheral nervous system</subject><subject>Peripheral Nervous System - metabolism</subject><subject>Protein Isoforms - analysis</subject><subject>Protein Isoforms - biosynthesis</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk1v1DAQhiMEotvCXyi-IE5ZxnbsdS6IqhRYqRKHlrPldSatl6wT7KTS8uuZsFuQuNQXy6NnPvy-UxTnHJYcuH6_XW6SCzFhXgr4E1xCLZ4VC25WotSigufFAgB0aepanhSnOW_pKWUNL4sTwaGqNFeLwn4KbYsJ4xhcx7reuy78cmPoI-tbFnFKeDd1IZacjfsB2Xq9ZiGy8R6Zp6RESS42bMAUhnucnxHTQz9llvd5xN2r4kXruoyvj_dZcfv56vbya3n97cv68uK69ArMWBqtjJScy4ZvlOONa5GvNECjawr5CoyslBOmrrTiG75q6gbQV0pxQX9U8qx4dyg7pP7nhHm0u5A9dp2LSMNYo0Gais7TpBBSCyPnmvpA-tTnnLC1Qwo7l_aWg51NsFv7aIKdTZjjZAIlnh9bTJsdNn_THlUn4O0RcJkEb5OLPuR_nFwpKRQQ9-bAta637i4R8_2GOilysia1NBEfDwSStg8Bk80-YPTYhIR-tE0fnp72w38lPPkdaK4fuMe87acUyTnLbRYW7M28VPNOcQBRSSXkb2e1xQ8</recordid><startdate>20110119</startdate><enddate>20110119</enddate><creator>Bare, Dan J</creator><creator>Becker-Catania, Sara G</creator><creator>DeVries, George H</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope></search><sort><creationdate>20110119</creationdate><title>Differential localization of neuregulin-1 type III in the central and peripheral nervous system</title><author>Bare, Dan J ; Becker-Catania, Sara G ; DeVries, George H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c508t-865833113d1b5a1dafe17600d693d1c408345a2894651b17d9d0ec4551200653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>adults</topic><topic>Animals</topic><topic>antibodies</topic><topic>Antibody Specificity</topic><topic>axons</topic><topic>Biological and medical sciences</topic><topic>brain</topic><topic>Central Nervous System - metabolism</topic><topic>Dendrite</topic><topic>dendrites</topic><topic>erbB</topic><topic>Female</topic><topic>Fluorescent Antibody Technique</topic><topic>fractionation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects. Models. Methods</topic><topic>Immunoblotting</topic><topic>Immunolocalization</topic><topic>Immunoprecipitation</topic><topic>Male</topic><topic>Mice</topic><topic>Myelination</topic><topic>nerve tissue</topic><topic>Neuregulin-1 - analysis</topic><topic>Neuregulin-1 - biosynthesis</topic><topic>Neurogenesis - physiology</topic><topic>Neurology</topic><topic>Neurons - metabolism</topic><topic>peripheral nervous system</topic><topic>Peripheral Nervous System - metabolism</topic><topic>Protein Isoforms - analysis</topic><topic>Protein Isoforms - biosynthesis</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bare, Dan J</creatorcontrib><creatorcontrib>Becker-Catania, Sara G</creatorcontrib><creatorcontrib>DeVries, George H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bare, Dan J</au><au>Becker-Catania, Sara G</au><au>DeVries, George H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential localization of neuregulin-1 type III in the central and peripheral nervous system</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>2011-01-19</date><risdate>2011</risdate><volume>1369</volume><issue>19</issue><spage>10</spage><epage>20</epage><pages>10-20</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>Abstract In the developing PNS, axonal neuregulin-1 (NRG1) type III is the key determinant for myelination. However, the specific role for NRG1 (III) in the CNS has not been established. To address this issue, isotype-specific antibodies were generated, characterized, and used for the immunofluorescent localization of NRG1 (III) in the developing and adult CNS of rat. In contrast to adult peripheral nerve, which showed robust axonal staining, no immunoreactivity was observed in CNS myelinated tracts during the period of active myelination or in the adult CNS. Surprisingly, NRG1 (III) was prominently expressed on dendrites and soma in both the developing and adult CNS. These findings were corroborated through the subcellular fractionation of adult rat brain combined with an immunoblotting analysis. The immunolocalization of NRG1 (III) suggests that it plays a novel role in the myelination fate of CNS axons possibly through undetermined roles in neuronal maturation, or dendritic development and activation.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>21044615</pmid><doi>10.1016/j.brainres.2010.10.092</doi><tpages>11</tpages></addata></record> |
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| subjects | adults Animals antibodies Antibody Specificity axons Biological and medical sciences brain Central Nervous System - metabolism Dendrite dendrites erbB Female Fluorescent Antibody Technique fractionation Fundamental and applied biological sciences. Psychology General aspects. Models. Methods Immunoblotting Immunolocalization Immunoprecipitation Male Mice Myelination nerve tissue Neuregulin-1 - analysis Neuregulin-1 - biosynthesis Neurogenesis - physiology Neurology Neurons - metabolism peripheral nervous system Peripheral Nervous System - metabolism Protein Isoforms - analysis Protein Isoforms - biosynthesis Rats Rats, Sprague-Dawley Vertebrates: nervous system and sense organs |
| title | Differential localization of neuregulin-1 type III in the central and peripheral nervous system |
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