Gibberella zeae chitin synthase genes, GzCHS5 and GzCHS7, are required for hyphal growth, perithecia formation, and pathogenicity
Gibberella zeae causes Fusarium head blight of cereal crops, and sexual spores of the fungus play an important role as primary inocula. We isolated a restriction enzyme-mediated integration (REMI) transformant, ZH431, of G. zeae with defects in perithecia formation and virulence. Integration of the...
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| Veröffentlicht in: | Current genetics 2009-08, Vol.55 (4), p.449-459 |
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| creator | Kim, Jung-Eun Lee, Hyo-Jin Lee, Jungkwan Kim, Ki Woo Yun, Sung-Hwan Shim, Won-Bo Lee, Yin-Won |
| description | Gibberella zeae causes Fusarium head blight of cereal crops, and sexual spores of the fungus play an important role as primary inocula. We isolated a restriction enzyme-mediated integration (REMI) transformant, ZH431, of G. zeae with defects in perithecia formation and virulence. Integration of the REMI vector resulted in disruption of GzCHS7 gene, which encodes a putative class VII chitin synthase with high similarity to Fusarium oxysporum ChsVb. A second chitin synthase, GzCHS5, is adjacently located in a head-to-head configuration with GzCHS7, and its deduced protein sequence showed similarity with a class V chitin synthase in F. oxysporum. Neither ΔGzChs5 nor ΔGzChs7 mutants produced perithecia or caused disease on barley heads. Microscopic observation revealed that both mutants formed balloon-shaped hyphae and intrahyphal hyphae and that cell wall rigidity of the mutants was weaker than that of the wild-type strain. Transcription profiles of GzCHS5 and GzCHS7 were not altered in ΔGzChs7 and ΔGzChs5, respectively, suggesting that transcription regulations of the genes are independent of each other. Our results demonstrate that GzCHS5 and GzCHS7 are indispensable for perithecia formation and pathogenicity as well as normal septa formation and hyphal growth in G. zeae. |
| doi_str_mv | 10.1007/s00294-009-0258-6 |
| format | Article |
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We isolated a restriction enzyme-mediated integration (REMI) transformant, ZH431, of G. zeae with defects in perithecia formation and virulence. Integration of the REMI vector resulted in disruption of GzCHS7 gene, which encodes a putative class VII chitin synthase with high similarity to Fusarium oxysporum ChsVb. A second chitin synthase, GzCHS5, is adjacently located in a head-to-head configuration with GzCHS7, and its deduced protein sequence showed similarity with a class V chitin synthase in F. oxysporum. Neither ΔGzChs5 nor ΔGzChs7 mutants produced perithecia or caused disease on barley heads. Microscopic observation revealed that both mutants formed balloon-shaped hyphae and intrahyphal hyphae and that cell wall rigidity of the mutants was weaker than that of the wild-type strain. Transcription profiles of GzCHS5 and GzCHS7 were not altered in ΔGzChs7 and ΔGzChs5, respectively, suggesting that transcription regulations of the genes are independent of each other. Our results demonstrate that GzCHS5 and GzCHS7 are indispensable for perithecia formation and pathogenicity as well as normal septa formation and hyphal growth in G. zeae.</description><identifier>ISSN: 0172-8083</identifier><identifier>EISSN: 1432-0983</identifier><identifier>DOI: 10.1007/s00294-009-0258-6</identifier><identifier>PMID: 19547974</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Amino acid sequence ; Biochemistry ; Biomedical and Life Sciences ; Blight ; Cell Biology ; Cell walls ; Cereal crops ; Cereals ; Chitin ; Chitin synthase ; Chitin Synthase - genetics ; Chitin Synthase - metabolism ; Crops ; Fusarium oxysporum ; Gene Expression Regulation, Fungal ; Genes, Fungal ; Gibberella - enzymology ; Gibberella - genetics ; Gibberella - pathogenicity ; Gibberella zeae ; Head ; Hordeum vulgare ; Hyphae ; Hyphae - genetics ; Hyphae - metabolism ; Integration ; Life Sciences ; Microbial Genetics and Genomics ; Microbiology ; Pathogenicity ; Pathogens ; Perithecia ; Plant Sciences ; Proteomics ; Research Article ; Rigidity ; Septum ; Spores ; Transcription ; Virulence</subject><ispartof>Current genetics, 2009-08, Vol.55 (4), p.449-459</ispartof><rights>Springer-Verlag 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c492t-da9032e3217e963c36bea9ae5f02ab49b7504b793702211a225eb47c742cea2b3</citedby><cites>FETCH-LOGICAL-c492t-da9032e3217e963c36bea9ae5f02ab49b7504b793702211a225eb47c742cea2b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00294-009-0258-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00294-009-0258-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27907,27908,41471,42540,51302</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19547974$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Jung-Eun</creatorcontrib><creatorcontrib>Lee, Hyo-Jin</creatorcontrib><creatorcontrib>Lee, Jungkwan</creatorcontrib><creatorcontrib>Kim, Ki Woo</creatorcontrib><creatorcontrib>Yun, Sung-Hwan</creatorcontrib><creatorcontrib>Shim, Won-Bo</creatorcontrib><creatorcontrib>Lee, Yin-Won</creatorcontrib><title>Gibberella zeae chitin synthase genes, GzCHS5 and GzCHS7, are required for hyphal growth, perithecia formation, and pathogenicity</title><title>Current genetics</title><addtitle>Curr Genet</addtitle><addtitle>Curr Genet</addtitle><description>Gibberella zeae causes Fusarium head blight of cereal crops, and sexual spores of the fungus play an important role as primary inocula. We isolated a restriction enzyme-mediated integration (REMI) transformant, ZH431, of G. zeae with defects in perithecia formation and virulence. Integration of the REMI vector resulted in disruption of GzCHS7 gene, which encodes a putative class VII chitin synthase with high similarity to Fusarium oxysporum ChsVb. A second chitin synthase, GzCHS5, is adjacently located in a head-to-head configuration with GzCHS7, and its deduced protein sequence showed similarity with a class V chitin synthase in F. oxysporum. Neither ΔGzChs5 nor ΔGzChs7 mutants produced perithecia or caused disease on barley heads. Microscopic observation revealed that both mutants formed balloon-shaped hyphae and intrahyphal hyphae and that cell wall rigidity of the mutants was weaker than that of the wild-type strain. Transcription profiles of GzCHS5 and GzCHS7 were not altered in ΔGzChs7 and ΔGzChs5, respectively, suggesting that transcription regulations of the genes are independent of each other. Our results demonstrate that GzCHS5 and GzCHS7 are indispensable for perithecia formation and pathogenicity as well as normal septa formation and hyphal growth in G. zeae.</description><subject>Amino acid sequence</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Blight</subject><subject>Cell Biology</subject><subject>Cell walls</subject><subject>Cereal crops</subject><subject>Cereals</subject><subject>Chitin</subject><subject>Chitin synthase</subject><subject>Chitin Synthase - genetics</subject><subject>Chitin Synthase - metabolism</subject><subject>Crops</subject><subject>Fusarium oxysporum</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Genes, Fungal</subject><subject>Gibberella - enzymology</subject><subject>Gibberella - genetics</subject><subject>Gibberella - pathogenicity</subject><subject>Gibberella zeae</subject><subject>Head</subject><subject>Hordeum vulgare</subject><subject>Hyphae</subject><subject>Hyphae - genetics</subject><subject>Hyphae - metabolism</subject><subject>Integration</subject><subject>Life Sciences</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Perithecia</subject><subject>Plant Sciences</subject><subject>Proteomics</subject><subject>Research Article</subject><subject>Rigidity</subject><subject>Septum</subject><subject>Spores</subject><subject>Transcription</subject><subject>Virulence</subject><issn>0172-8083</issn><issn>1432-0983</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU1v1DAQhi0EokvhB3ABiwuXDYw_EsdHtIItUiUOpWdrkp1sXO0mqZ2o2t745zhkpUoc4GRL87zvaPQw9lbAJwFgPkcAaXUGYDOQeZkVz9hKaCUzsKV6zlYgjMxKKNUFexXjHYCQpTUv2YWwuTbW6BX7tfVVRYEOB-SPhMTr1o--4_HUjS1G4nvqKK759nFzdZNz7HbL16w5BuKB7icfaMebPvD2NLR44PvQP4ztmg8U_NhS7XGeHnH0fbf-0zDg2Pap2Nd-PL1mLxo8RHpzfi_Z7bevPzdX2fWP7ffNl-us1laO2Q4tKElKCkO2ULUqKkKLlDcgsdK2MjnoylhlQEohUMqcKm1qo2VNKCt1yT4uvUPo7yeKozv6WM-Hd9RP0ZUFqBJAi_-SRqnclCqfyQ9_kXf9FLp0hkv7k4m81AkSC1SHPsZAjRuCP2I4OQFu9ugWjy55dLNHV6TMu3PxVB1p95Q4i0uAXICYRt2ewtPmf7W-X0IN9g73wUd3eyNBKBBFYcCA-g1BKLBx</recordid><startdate>20090801</startdate><enddate>20090801</enddate><creator>Kim, Jung-Eun</creator><creator>Lee, Hyo-Jin</creator><creator>Lee, Jungkwan</creator><creator>Kim, Ki Woo</creator><creator>Yun, Sung-Hwan</creator><creator>Shim, Won-Bo</creator><creator>Lee, Yin-Won</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20090801</creationdate><title>Gibberella zeae chitin synthase genes, GzCHS5 and GzCHS7, are required for hyphal growth, perithecia formation, and pathogenicity</title><author>Kim, Jung-Eun ; Lee, Hyo-Jin ; Lee, Jungkwan ; Kim, Ki Woo ; Yun, Sung-Hwan ; Shim, Won-Bo ; Lee, Yin-Won</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c492t-da9032e3217e963c36bea9ae5f02ab49b7504b793702211a225eb47c742cea2b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Amino acid sequence</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Blight</topic><topic>Cell Biology</topic><topic>Cell walls</topic><topic>Cereal crops</topic><topic>Cereals</topic><topic>Chitin</topic><topic>Chitin synthase</topic><topic>Chitin Synthase - genetics</topic><topic>Chitin Synthase - metabolism</topic><topic>Crops</topic><topic>Fusarium oxysporum</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Genes, Fungal</topic><topic>Gibberella - enzymology</topic><topic>Gibberella - genetics</topic><topic>Gibberella - pathogenicity</topic><topic>Gibberella zeae</topic><topic>Head</topic><topic>Hordeum vulgare</topic><topic>Hyphae</topic><topic>Hyphae - genetics</topic><topic>Hyphae - metabolism</topic><topic>Integration</topic><topic>Life Sciences</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Perithecia</topic><topic>Plant Sciences</topic><topic>Proteomics</topic><topic>Research Article</topic><topic>Rigidity</topic><topic>Septum</topic><topic>Spores</topic><topic>Transcription</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Jung-Eun</creatorcontrib><creatorcontrib>Lee, Hyo-Jin</creatorcontrib><creatorcontrib>Lee, Jungkwan</creatorcontrib><creatorcontrib>Kim, Ki Woo</creatorcontrib><creatorcontrib>Yun, Sung-Hwan</creatorcontrib><creatorcontrib>Shim, Won-Bo</creatorcontrib><creatorcontrib>Lee, Yin-Won</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Current genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Jung-Eun</au><au>Lee, Hyo-Jin</au><au>Lee, Jungkwan</au><au>Kim, Ki Woo</au><au>Yun, Sung-Hwan</au><au>Shim, Won-Bo</au><au>Lee, Yin-Won</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gibberella zeae chitin synthase genes, GzCHS5 and GzCHS7, are required for hyphal growth, perithecia formation, and pathogenicity</atitle><jtitle>Current genetics</jtitle><stitle>Curr Genet</stitle><addtitle>Curr Genet</addtitle><date>2009-08-01</date><risdate>2009</risdate><volume>55</volume><issue>4</issue><spage>449</spage><epage>459</epage><pages>449-459</pages><issn>0172-8083</issn><eissn>1432-0983</eissn><abstract>Gibberella zeae causes Fusarium head blight of cereal crops, and sexual spores of the fungus play an important role as primary inocula. We isolated a restriction enzyme-mediated integration (REMI) transformant, ZH431, of G. zeae with defects in perithecia formation and virulence. Integration of the REMI vector resulted in disruption of GzCHS7 gene, which encodes a putative class VII chitin synthase with high similarity to Fusarium oxysporum ChsVb. A second chitin synthase, GzCHS5, is adjacently located in a head-to-head configuration with GzCHS7, and its deduced protein sequence showed similarity with a class V chitin synthase in F. oxysporum. Neither ΔGzChs5 nor ΔGzChs7 mutants produced perithecia or caused disease on barley heads. Microscopic observation revealed that both mutants formed balloon-shaped hyphae and intrahyphal hyphae and that cell wall rigidity of the mutants was weaker than that of the wild-type strain. Transcription profiles of GzCHS5 and GzCHS7 were not altered in ΔGzChs7 and ΔGzChs5, respectively, suggesting that transcription regulations of the genes are independent of each other. Our results demonstrate that GzCHS5 and GzCHS7 are indispensable for perithecia formation and pathogenicity as well as normal septa formation and hyphal growth in G. zeae.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>19547974</pmid><doi>10.1007/s00294-009-0258-6</doi><tpages>11</tpages></addata></record> |
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| subjects | Amino acid sequence Biochemistry Biomedical and Life Sciences Blight Cell Biology Cell walls Cereal crops Cereals Chitin Chitin synthase Chitin Synthase - genetics Chitin Synthase - metabolism Crops Fusarium oxysporum Gene Expression Regulation, Fungal Genes, Fungal Gibberella - enzymology Gibberella - genetics Gibberella - pathogenicity Gibberella zeae Head Hordeum vulgare Hyphae Hyphae - genetics Hyphae - metabolism Integration Life Sciences Microbial Genetics and Genomics Microbiology Pathogenicity Pathogens Perithecia Plant Sciences Proteomics Research Article Rigidity Septum Spores Transcription Virulence |
| title | Gibberella zeae chitin synthase genes, GzCHS5 and GzCHS7, are required for hyphal growth, perithecia formation, and pathogenicity |
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