Qualitative and quantitative thrombin analysis in serum using aptamers and gold nanoparticles through progressive dilution
It has been a long-standing challenge in bioassay using aptamers and gold nanoparticles to detect disease-related proteins and other substance directly in complex biological samples such as serum. Here we propose a progressive dilution (PD) method to achieve simultaneous qualitative and quantitative...
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| Veröffentlicht in: | Analytical biochemistry 2011-06, Vol.413 (1), p.75-77 |
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| container_end_page | 77 |
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| container_title | Analytical biochemistry |
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| creator | Ge, Jing Liu, Zhaofang Zhi, Zeyong Zhao, Xin Sheng |
| description | It has been a long-standing challenge in bioassay using aptamers and gold nanoparticles to detect disease-related proteins and other substance directly in complex biological samples such as serum. Here we propose a progressive dilution (PD) method to achieve simultaneous qualitative and quantitative analysis of proteins in blood serum without pretreatment of the sample. Above the detection limit, PD has unlimited dynamic range. We demonstrate the PD strategy through the detection of thrombin in fetal bovine serum using the quenching of fluorescence by gold nanoparticles. |
| doi_str_mv | 10.1016/j.ab.2011.01.032 |
| format | Article |
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Here we propose a progressive dilution (PD) method to achieve simultaneous qualitative and quantitative analysis of proteins in blood serum without pretreatment of the sample. Above the detection limit, PD has unlimited dynamic range. We demonstrate the PD strategy through the detection of thrombin in fetal bovine serum using the quenching of fluorescence by gold nanoparticles.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/j.ab.2011.01.032</identifier><identifier>PMID: 21284928</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Aptamers, Nucleotide - chemistry ; bioassays ; blood serum ; Bovines ; Cattle ; detection limit ; fetal bovine serum ; fluorescence ; Gold - chemistry ; Metal Nanoparticles - chemistry ; nanogold ; oligonucleotides ; proteins ; quantitative analysis ; Spectrometry, Fluorescence - methods ; thrombin ; Thrombin - analysis</subject><ispartof>Analytical biochemistry, 2011-06, Vol.413 (1), p.75-77</ispartof><rights>2011 Elsevier Inc.</rights><rights>Copyright © 2011 Elsevier Inc. 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We demonstrate the PD strategy through the detection of thrombin in fetal bovine serum using the quenching of fluorescence by gold nanoparticles.</description><subject>Animals</subject><subject>Aptamers, Nucleotide - chemistry</subject><subject>bioassays</subject><subject>blood serum</subject><subject>Bovines</subject><subject>Cattle</subject><subject>detection limit</subject><subject>fetal bovine serum</subject><subject>fluorescence</subject><subject>Gold - chemistry</subject><subject>Metal Nanoparticles - chemistry</subject><subject>nanogold</subject><subject>oligonucleotides</subject><subject>proteins</subject><subject>quantitative analysis</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>thrombin</subject><subject>Thrombin - analysis</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtv1DAUhS0EosPAnhVkB5sM1484Nruq4iVVQgi6thzHST1K7Kkflcqvx9Np2YF0JVv2d869ugeh1xh2GDD_sN_pYUcA4x3UouQJ2mCQvAUK8inaAABtCZf9GXqR0h4qyDr-HJ0RTASTRGzQ7x9FLy7r7G5to_3Y3BTt8-NDvo5hHZyvP3q5Sy419Z5sLGtTkvNzow9Zrzame-kclrHx2oeDjtmZxaZ7gzJfN4cY5mhTOpqObinZBf8SPZv0kuyrh3OLrj5_-nXxtb38_uXbxflla1hPcouHjgs-UNZNbOrHjpFeSDERBiANBwJmkD2VhHVsFCPDdDKcEoFh0noydqBb9O7kW4e4KTZltbpk7LJob0NJSnDAvQDZVfL9f8m68152jNbaIjihJoaUop3UIbpVx7sKHTmu9koP6piNglqUVMmbB_cyrHb8K3gMowJvT8Ckg9JzdEld_awOvOZIpOiPA348Ebbu69bZqJJx1hs7umhNVmNw_-7_B9ZFqTc</recordid><startdate>20110601</startdate><enddate>20110601</enddate><creator>Ge, Jing</creator><creator>Liu, Zhaofang</creator><creator>Zhi, Zeyong</creator><creator>Zhao, Xin Sheng</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20110601</creationdate><title>Qualitative and quantitative thrombin analysis in serum using aptamers and gold nanoparticles through progressive dilution</title><author>Ge, Jing ; Liu, Zhaofang ; Zhi, Zeyong ; Zhao, Xin Sheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c472t-1b5686b345f4f7d5427898f24009c6020cb97392454d8d413fc632810faafceb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Aptamers, Nucleotide - chemistry</topic><topic>bioassays</topic><topic>blood serum</topic><topic>Bovines</topic><topic>Cattle</topic><topic>detection limit</topic><topic>fetal bovine serum</topic><topic>fluorescence</topic><topic>Gold - chemistry</topic><topic>Metal Nanoparticles - chemistry</topic><topic>nanogold</topic><topic>oligonucleotides</topic><topic>proteins</topic><topic>quantitative analysis</topic><topic>Spectrometry, Fluorescence - methods</topic><topic>thrombin</topic><topic>Thrombin - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ge, Jing</creatorcontrib><creatorcontrib>Liu, Zhaofang</creatorcontrib><creatorcontrib>Zhi, Zeyong</creatorcontrib><creatorcontrib>Zhao, Xin Sheng</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ge, Jing</au><au>Liu, Zhaofang</au><au>Zhi, Zeyong</au><au>Zhao, Xin Sheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Qualitative and quantitative thrombin analysis in serum using aptamers and gold nanoparticles through progressive dilution</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2011-06-01</date><risdate>2011</risdate><volume>413</volume><issue>1</issue><spage>75</spage><epage>77</epage><pages>75-77</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>It has been a long-standing challenge in bioassay using aptamers and gold nanoparticles to detect disease-related proteins and other substance directly in complex biological samples such as serum. 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| ispartof | Analytical biochemistry, 2011-06, Vol.413 (1), p.75-77 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Animals Aptamers, Nucleotide - chemistry bioassays blood serum Bovines Cattle detection limit fetal bovine serum fluorescence Gold - chemistry Metal Nanoparticles - chemistry nanogold oligonucleotides proteins quantitative analysis Spectrometry, Fluorescence - methods thrombin Thrombin - analysis |
| title | Qualitative and quantitative thrombin analysis in serum using aptamers and gold nanoparticles through progressive dilution |
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