Quantitative 3D profilometry and SEM analysis of the adaptation of root-end filling materials placed under an optical microscope

Munhoz MF, Marchesan MA, Cardoso DRF, Silva SRC, Silva‐Sousa YTC, Sousa‐Neto MD. Quantitative 3D profilometry and SEM analysis of the adaptation of root‐end filling materials placed under an optical microscope. International Endodontic Journal, 44, 560–566, 2011. Aim To evaluate by 3D profilometry and scanning electron microscopy (SEM), the marginal adaptation of mineral trioxide aggregate (MTA) and Sealer 26 placed in root‐end cavities with direct vision or under an optical microscope. Methodology The root ends of 52 root filled canine teeth were filled with MTA or Sealer 26 under direct vision or optical microscope (n = 13). In each group, eight specimens were analysed by profilometry for measurement of the area and depth of gaps. In the other five specimens, gap area was measured using SEM to verify marginal adaptation and surface characteristic. Data were analysed by parametric (anova and Tukey) and non‐parametric (Kruskal–Wallis and Dunn) tests. Results The assessment of the adaptation of both materials to dentine was not influenced by the mode of visualization, which was confirmed by both profilometry and SEM observations. The voids measured with profilometry for Sealer 26 under direct vision were significantly wider and deeper than those for MTA under direct vision (P