Development and Application of Stir Bar Sorptive Extraction with Polyurethane Foams for the Determination of Testosterone and Methenolone in Urine Matrices
This work describes the development, validation, and application of a novel methodology for the determination of testosterone and methenolone in urine matrices by stir bar sorptive extraction using polyurethane foams [SBSE(PU)] followed by liquid desorption and high-performance liquid chromatography...
Gespeichert in:
| Veröffentlicht in: | Journal of chromatographic science 2011-04, Vol.49 (4), p.297-302 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 302 |
|---|---|
| container_issue | 4 |
| container_start_page | 297 |
| container_title | Journal of chromatographic science |
| container_volume | 49 |
| creator | Sequeiros, R.C.P. Neng, N.R. Portugal, F.C.M. Pinto, M.L. Pires, J. Nogueira, J.M.F. |
| description | This work describes the development, validation, and application of a novel methodology for the determination of testosterone and methenolone in urine matrices by stir bar sorptive extraction using polyurethane foams [SBSE(PU)] followed by liquid desorption and high-performance liquid chromatography with diode array detection. The methodology was optimized in terms of extraction time, agitation speed, pH, ionic strength and organic modifier, as well as back-extraction solvent and desorption time. Under optimized experimental conditions, convenient accuracy were achieved with average recoveries of 49.7 ± 8.6% for testosterone and 54.2 ± 4.7% for methenolone. Additionally, the methodology showed good precision (< 9%), excellent linear dynamic ranges (> 0.9963) and convenient detection limits (0.2-0.3 µg/L). When comparing the efficiency obtained by SBSE(PU) and with the conventional polydimethylsiloxane phase [SBSE(PDMS)], yields up to four-fold higher are attained for the former, under the same experimental conditions. The application of the proposed methodology for the analysis of testosterone and methenolone in urine matrices showed negligible matrix effects and good analytical performance. |
| doi_str_mv | 10.1093/chrsci/49.4.297 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_859058831</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/chrsci/49.4.297</oup_id><sourcerecordid>859058831</sourcerecordid><originalsourceid>FETCH-LOGICAL-c402t-f7ccb8c1b91d54ec1d685e5897ceec5ce3f61e2d02a988ac92e8f3c0aeba67423</originalsourceid><addsrcrecordid>eNqFkU9v1DAQxS0EokvhzA35gpCQsms7dmIf-5citQKprcQt8k4mWqMkDrZT6Gfhy-Jlt-2xJ3vGv3lvrEfIe86WnJlyBZsQwa2kWcqlMPULsuBGqkKU5sdLsmBM8MJUlTogb2L8uS25Vq_JgeCyNFzwBfl7infY-2nAMVE7tvRomnoHNjk_Ut_R6-QCPbaBXvswJXeH9OxPChb-v_92aUO_-_5-Dpg2dkR67u0QaecDTRukp5gwDG58VLvBmHzMTZ_ZrdtVnsPR99vajfQ2uHy5sik4wPiWvOpsH_Hd_jwkt-dnNycXxeW3L19Pji4LkEykoqsB1hr42vBWSQTeVlqh0qYGRFCAZVdxFC0T1mhtwQjUXQnM4tpWtRTlIfm0052C_zXnFZvBRcC-zz_yc2y0MkxpXfJMrnYkBB9jwK6ZghtsuG84a7aBNLtAGmka2eRA8sSHvfa8HrB95B8SyMDHPWAj2L4LdgQXn7jS1HWpZeY-7zg_T8-6_gPp3qd-</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>859058831</pqid></control><display><type>article</type><title>Development and Application of Stir Bar Sorptive Extraction with Polyurethane Foams for the Determination of Testosterone and Methenolone in Urine Matrices</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Free Full-Text Journals in Chemistry</source><creator>Sequeiros, R.C.P. ; Neng, N.R. ; Portugal, F.C.M. ; Pinto, M.L. ; Pires, J. ; Nogueira, J.M.F.</creator><creatorcontrib>Sequeiros, R.C.P. ; Neng, N.R. ; Portugal, F.C.M. ; Pinto, M.L. ; Pires, J. ; Nogueira, J.M.F.</creatorcontrib><description>This work describes the development, validation, and application of a novel methodology for the determination of testosterone and methenolone in urine matrices by stir bar sorptive extraction using polyurethane foams [SBSE(PU)] followed by liquid desorption and high-performance liquid chromatography with diode array detection. The methodology was optimized in terms of extraction time, agitation speed, pH, ionic strength and organic modifier, as well as back-extraction solvent and desorption time. Under optimized experimental conditions, convenient accuracy were achieved with average recoveries of 49.7 ± 8.6% for testosterone and 54.2 ± 4.7% for methenolone. Additionally, the methodology showed good precision (< 9%), excellent linear dynamic ranges (> 0.9963) and convenient detection limits (0.2-0.3 µg/L). When comparing the efficiency obtained by SBSE(PU) and with the conventional polydimethylsiloxane phase [SBSE(PDMS)], yields up to four-fold higher are attained for the former, under the same experimental conditions. The application of the proposed methodology for the analysis of testosterone and methenolone in urine matrices showed negligible matrix effects and good analytical performance.</description><identifier>ISSN: 0021-9665</identifier><identifier>EISSN: 1945-239X</identifier><identifier>DOI: 10.1093/chrsci/49.4.297</identifier><identifier>PMID: 21439121</identifier><identifier>CODEN: JCHSBZ</identifier><language>eng</language><publisher>Niles, IL: Oxford University Press</publisher><subject>Adult ; Analysis ; Biological and medical sciences ; Chemical Fractionation - methods ; Chromatography, High Pressure Liquid ; General pharmacology ; Humans ; Hydrogen-Ion Concentration ; Male ; Medical sciences ; Methenolone - urine ; Osmolar Concentration ; Pharmacology. Drug treatments ; Polyurethanes - chemistry ; Reproducibility of Results ; Sensitivity and Specificity ; Testosterone - urine</subject><ispartof>Journal of chromatographic science, 2011-04, Vol.49 (4), p.297-302</ispartof><rights>2011</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-f7ccb8c1b91d54ec1d685e5897ceec5ce3f61e2d02a988ac92e8f3c0aeba67423</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1583,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23977384$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21439121$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sequeiros, R.C.P.</creatorcontrib><creatorcontrib>Neng, N.R.</creatorcontrib><creatorcontrib>Portugal, F.C.M.</creatorcontrib><creatorcontrib>Pinto, M.L.</creatorcontrib><creatorcontrib>Pires, J.</creatorcontrib><creatorcontrib>Nogueira, J.M.F.</creatorcontrib><title>Development and Application of Stir Bar Sorptive Extraction with Polyurethane Foams for the Determination of Testosterone and Methenolone in Urine Matrices</title><title>Journal of chromatographic science</title><addtitle>Journal of Chromatographic Science</addtitle><addtitle>J Chromatogr Sci</addtitle><description>This work describes the development, validation, and application of a novel methodology for the determination of testosterone and methenolone in urine matrices by stir bar sorptive extraction using polyurethane foams [SBSE(PU)] followed by liquid desorption and high-performance liquid chromatography with diode array detection. The methodology was optimized in terms of extraction time, agitation speed, pH, ionic strength and organic modifier, as well as back-extraction solvent and desorption time. Under optimized experimental conditions, convenient accuracy were achieved with average recoveries of 49.7 ± 8.6% for testosterone and 54.2 ± 4.7% for methenolone. Additionally, the methodology showed good precision (< 9%), excellent linear dynamic ranges (> 0.9963) and convenient detection limits (0.2-0.3 µg/L). When comparing the efficiency obtained by SBSE(PU) and with the conventional polydimethylsiloxane phase [SBSE(PDMS)], yields up to four-fold higher are attained for the former, under the same experimental conditions. The application of the proposed methodology for the analysis of testosterone and methenolone in urine matrices showed negligible matrix effects and good analytical performance.</description><subject>Adult</subject><subject>Analysis</subject><subject>Biological and medical sciences</subject><subject>Chemical Fractionation - methods</subject><subject>Chromatography, High Pressure Liquid</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Methenolone - urine</subject><subject>Osmolar Concentration</subject><subject>Pharmacology. Drug treatments</subject><subject>Polyurethanes - chemistry</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Testosterone - urine</subject><issn>0021-9665</issn><issn>1945-239X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EokvhzA35gpCQsms7dmIf-5citQKprcQt8k4mWqMkDrZT6Gfhy-Jlt-2xJ3vGv3lvrEfIe86WnJlyBZsQwa2kWcqlMPULsuBGqkKU5sdLsmBM8MJUlTogb2L8uS25Vq_JgeCyNFzwBfl7infY-2nAMVE7tvRomnoHNjk_Ut_R6-QCPbaBXvswJXeH9OxPChb-v_92aUO_-_5-Dpg2dkR67u0QaecDTRukp5gwDG58VLvBmHzMTZ_ZrdtVnsPR99vajfQ2uHy5sik4wPiWvOpsH_Hd_jwkt-dnNycXxeW3L19Pji4LkEykoqsB1hr42vBWSQTeVlqh0qYGRFCAZVdxFC0T1mhtwQjUXQnM4tpWtRTlIfm0052C_zXnFZvBRcC-zz_yc2y0MkxpXfJMrnYkBB9jwK6ZghtsuG84a7aBNLtAGmka2eRA8sSHvfa8HrB95B8SyMDHPWAj2L4LdgQXn7jS1HWpZeY-7zg_T8-6_gPp3qd-</recordid><startdate>20110401</startdate><enddate>20110401</enddate><creator>Sequeiros, R.C.P.</creator><creator>Neng, N.R.</creator><creator>Portugal, F.C.M.</creator><creator>Pinto, M.L.</creator><creator>Pires, J.</creator><creator>Nogueira, J.M.F.</creator><general>Oxford University Press</general><general>Preston Publications</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110401</creationdate><title>Development and Application of Stir Bar Sorptive Extraction with Polyurethane Foams for the Determination of Testosterone and Methenolone in Urine Matrices</title><author>Sequeiros, R.C.P. ; Neng, N.R. ; Portugal, F.C.M. ; Pinto, M.L. ; Pires, J. ; Nogueira, J.M.F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-f7ccb8c1b91d54ec1d685e5897ceec5ce3f61e2d02a988ac92e8f3c0aeba67423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adult</topic><topic>Analysis</topic><topic>Biological and medical sciences</topic><topic>Chemical Fractionation - methods</topic><topic>Chromatography, High Pressure Liquid</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Methenolone - urine</topic><topic>Osmolar Concentration</topic><topic>Pharmacology. Drug treatments</topic><topic>Polyurethanes - chemistry</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Testosterone - urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sequeiros, R.C.P.</creatorcontrib><creatorcontrib>Neng, N.R.</creatorcontrib><creatorcontrib>Portugal, F.C.M.</creatorcontrib><creatorcontrib>Pinto, M.L.</creatorcontrib><creatorcontrib>Pires, J.</creatorcontrib><creatorcontrib>Nogueira, J.M.F.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatographic science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sequeiros, R.C.P.</au><au>Neng, N.R.</au><au>Portugal, F.C.M.</au><au>Pinto, M.L.</au><au>Pires, J.</au><au>Nogueira, J.M.F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and Application of Stir Bar Sorptive Extraction with Polyurethane Foams for the Determination of Testosterone and Methenolone in Urine Matrices</atitle><jtitle>Journal of chromatographic science</jtitle><stitle>Journal of Chromatographic Science</stitle><addtitle>J Chromatogr Sci</addtitle><date>2011-04-01</date><risdate>2011</risdate><volume>49</volume><issue>4</issue><spage>297</spage><epage>302</epage><pages>297-302</pages><issn>0021-9665</issn><eissn>1945-239X</eissn><coden>JCHSBZ</coden><abstract>This work describes the development, validation, and application of a novel methodology for the determination of testosterone and methenolone in urine matrices by stir bar sorptive extraction using polyurethane foams [SBSE(PU)] followed by liquid desorption and high-performance liquid chromatography with diode array detection. The methodology was optimized in terms of extraction time, agitation speed, pH, ionic strength and organic modifier, as well as back-extraction solvent and desorption time. Under optimized experimental conditions, convenient accuracy were achieved with average recoveries of 49.7 ± 8.6% for testosterone and 54.2 ± 4.7% for methenolone. Additionally, the methodology showed good precision (< 9%), excellent linear dynamic ranges (> 0.9963) and convenient detection limits (0.2-0.3 µg/L). When comparing the efficiency obtained by SBSE(PU) and with the conventional polydimethylsiloxane phase [SBSE(PDMS)], yields up to four-fold higher are attained for the former, under the same experimental conditions. The application of the proposed methodology for the analysis of testosterone and methenolone in urine matrices showed negligible matrix effects and good analytical performance.</abstract><cop>Niles, IL</cop><pub>Oxford University Press</pub><pmid>21439121</pmid><doi>10.1093/chrsci/49.4.297</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9665 |
| ispartof | Journal of chromatographic science, 2011-04, Vol.49 (4), p.297-302 |
| issn | 0021-9665 1945-239X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_859058831 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Free Full-Text Journals in Chemistry |
| subjects | Adult Analysis Biological and medical sciences Chemical Fractionation - methods Chromatography, High Pressure Liquid General pharmacology Humans Hydrogen-Ion Concentration Male Medical sciences Methenolone - urine Osmolar Concentration Pharmacology. Drug treatments Polyurethanes - chemistry Reproducibility of Results Sensitivity and Specificity Testosterone - urine |
| title | Development and Application of Stir Bar Sorptive Extraction with Polyurethane Foams for the Determination of Testosterone and Methenolone in Urine Matrices |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T18%3A17%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20and%20Application%20of%20Stir%20Bar%20Sorptive%20Extraction%20with%20Polyurethane%20Foams%20for%20the%20Determination%20of%20Testosterone%20and%20Methenolone%20in%20Urine%20Matrices&rft.jtitle=Journal%20of%20chromatographic%20science&rft.au=Sequeiros,%20R.C.P.&rft.date=2011-04-01&rft.volume=49&rft.issue=4&rft.spage=297&rft.epage=302&rft.pages=297-302&rft.issn=0021-9665&rft.eissn=1945-239X&rft.coden=JCHSBZ&rft_id=info:doi/10.1093/chrsci/49.4.297&rft_dat=%3Cproquest_cross%3E859058831%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=859058831&rft_id=info:pmid/21439121&rft_oup_id=10.1093/chrsci/49.4.297&rfr_iscdi=true |