Origin and hierarchy of basal lamina-forming and -non-forming myogenic cells in mouse skeletal muscle in relation to adhesive capacity and Pax7 expression in vitro
As a novel approach to distinguish skeletal myogenic cell populations, basal lamina (BL) formation of myogenic cells was examined in the mouse compensatory enlarged plantaris muscles in vivo and in fiber-bundle cultures in vitro. MyoD + myogenic cells located inside the regenerative muscle fiber BL...
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| Veröffentlicht in: | Cell and tissue research 2011-04, Vol.344 (1), p.147-168 |
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| creator | Tamaki, Tetsuro Tono, Kayoko Uchiyama, Yoshiyasu Okada, Yoshinori Masuda, Maki Soeda, Shuichi Nitta, Masahiro Akatsuka, Akira |
| description | As a novel approach to distinguish skeletal myogenic cell populations, basal lamina (BL) formation of myogenic cells was examined in the mouse compensatory enlarged plantaris muscles in vivo and in fiber-bundle cultures in vitro. MyoD
+
myogenic cells located inside the regenerative muscle fiber BL were laminin
−
but interstitial MyoD
+
cells were laminin
+
. This was also confirmed by electron microscopy as structural BL formation. Similar trends were observed in the fiber-bundle cultures including satellite cells and interstitial myogenic cells and laminin
+
myogenic cells predominantly showed non-adhesive (non-Ad) behavior with Pax7
−
, whereas laminin
−
cells were adhesive (Ad) with Pax7
+
. Moreover, non-Ad/laminin
+
and Ad/laminin
−
myotubes were also observed and the former type showed spontaneous contractions, while the latter type did not. The origin and hierarchy of Ad/Pax7
+
/laminin
−
and non-Ad/Pax7
−
/laminin
+
myogenic cells were also examined using skeletal muscle interstitium-derived CD34
+
/45
−
(Sk-34) and CD34
−
/45
−
(Sk-DN) multipotent stem cells, which were composed of non-committed myogenic cells with a few ( |
| doi_str_mv | 10.1007/s00441-010-1127-9 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_858783260</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A354859314</galeid><sourcerecordid>A354859314</sourcerecordid><originalsourceid>FETCH-LOGICAL-c468t-b04e5cde33dbcd470ee7f550c42b1a47ee2ba5d3b2a11bdb6c484a0c57e48ced3</originalsourceid><addsrcrecordid>eNp1ks9u1DAQxi0EosvCA3BBFpXglGI7Tpw9VhX_pErlABK3aOJMEhfHXuyk6j4PL4rTlEIRyIeRZ37fJ894CHnO2QlnTL2JjEnJM8ZZxrlQ2e4B2XCZi4xVqnpINixnIlNl-fWIPInxkjEuy3L3mByJRMuiVBvy4yKY3jgKrqWDwQBBDwfqO9pABEstjMZB1vmQYn9DZc67u8R48D06o6lGayNNRqOfI9L4DS1OyWCco7a4FAJamIx3dPIU2gGjuUKqYQ_aTIcb509wrShe7wPGuIBJdGWm4J-SRx3YiM9u45Z8eff289mH7Pzi_cez0_NMy7KasoZJLHSLed42upWKIaquKJiWouEgFaJooGjzRgDnTduUWlYSmC4Uykpjm2_J69V3H_z3GeNUjyYujYHD1FVdFWmsuShZIl_-RV76Obj0uAUSSnBWJeh4hXqwWBvX-SmAXizr07yQVbHL02dtyck_qHRaHI32DjuT8vcEr_4QDAh2GqK38zLbeB_kK6iDjzFgV--DGSEcas7qZX_qdX9qttzTRtS7pHlx29jcjNjeKX4tTALECsRUcj2G353_3_Unzc3RWg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>858272108</pqid></control><display><type>article</type><title>Origin and hierarchy of basal lamina-forming and -non-forming myogenic cells in mouse skeletal muscle in relation to adhesive capacity and Pax7 expression in vitro</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Tamaki, Tetsuro ; Tono, Kayoko ; Uchiyama, Yoshiyasu ; Okada, Yoshinori ; Masuda, Maki ; Soeda, Shuichi ; Nitta, Masahiro ; Akatsuka, Akira</creator><creatorcontrib>Tamaki, Tetsuro ; Tono, Kayoko ; Uchiyama, Yoshiyasu ; Okada, Yoshinori ; Masuda, Maki ; Soeda, Shuichi ; Nitta, Masahiro ; Akatsuka, Akira</creatorcontrib><description>As a novel approach to distinguish skeletal myogenic cell populations, basal lamina (BL) formation of myogenic cells was examined in the mouse compensatory enlarged plantaris muscles in vivo and in fiber-bundle cultures in vitro. MyoD
+
myogenic cells located inside the regenerative muscle fiber BL were laminin
−
but interstitial MyoD
+
cells were laminin
+
. This was also confirmed by electron microscopy as structural BL formation. Similar trends were observed in the fiber-bundle cultures including satellite cells and interstitial myogenic cells and laminin
+
myogenic cells predominantly showed non-adhesive (non-Ad) behavior with Pax7
−
, whereas laminin
−
cells were adhesive (Ad) with Pax7
+
. Moreover, non-Ad/laminin
+
and Ad/laminin
−
myotubes were also observed and the former type showed spontaneous contractions, while the latter type did not. The origin and hierarchy of Ad/Pax7
+
/laminin
−
and non-Ad/Pax7
−
/laminin
+
myogenic cells were also examined using skeletal muscle interstitium-derived CD34
+
/45
−
(Sk-34) and CD34
−
/45
−
(Sk-DN) multipotent stem cells, which were composed of non-committed myogenic cells with a few (<1%) Pax7
+
cells in the Sk-DN cells at fresh isolation. Both cell types were separated by Ad/non-Ad capacity in repetitive culture. As expected, both Ad/Pax7
+
/laminin
−
and non-Ad/Pax7
−
/laminin
+
myogenic cells consistently appeared in the Ad and non-Ad cell culture. However, Ad/Pax7
+
/laminin
−
cells were repeatedly detected in the non-Ad cell culture, while the opposite phenomenon did not occur. This indicates that the source of non-Ad/ Pax7
−
/laminin
+
myogenic cells was present in the Sk-34 and Sk-DN stem cells and they were able to produce Ad/ Pax7
+
/ laminin
−
myogenic cells during myogenesis as primary myoblasts and situated hierarchically upstream of the latter cells.</description><identifier>ISSN: 0302-766X</identifier><identifier>EISSN: 1432-0878</identifier><identifier>DOI: 10.1007/s00441-010-1127-9</identifier><identifier>PMID: 21274567</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Animals ; Biomedical and Life Sciences ; Biomedicine ; Cell Adhesion ; Cell adhesion & migration ; Cell Line ; Cells, Cultured ; Desmin - analysis ; Desmin - genetics ; Electron microscopy ; Gene expression ; Gene Expression Regulation, Developmental ; Human Genetics ; Integrin beta1 - genetics ; Laminin - analysis ; Laminin - genetics ; Mice ; Mice, Inbred C57BL ; Molecular Medicine ; Muscle Development ; Muscle Fibers, Skeletal - cytology ; Muscle Fibers, Skeletal - metabolism ; Muscle, Skeletal - cytology ; Muscle, Skeletal - ultrastructure ; Muscles ; Musculoskeletal system ; MyoD Protein - analysis ; MyoD Protein - genetics ; Myogenin - analysis ; Myogenin - genetics ; PAX7 Transcription Factor - analysis ; PAX7 Transcription Factor - genetics ; Proteins ; Proteomics ; Regular Article ; Reverse Transcriptase Polymerase Chain Reaction ; Rodents</subject><ispartof>Cell and tissue research, 2011-04, Vol.344 (1), p.147-168</ispartof><rights>Springer-Verlag 2010</rights><rights>COPYRIGHT 2011 Springer</rights><rights>Springer-Verlag 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-b04e5cde33dbcd470ee7f550c42b1a47ee2ba5d3b2a11bdb6c484a0c57e48ced3</citedby><cites>FETCH-LOGICAL-c468t-b04e5cde33dbcd470ee7f550c42b1a47ee2ba5d3b2a11bdb6c484a0c57e48ced3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00441-010-1127-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00441-010-1127-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21274567$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tamaki, Tetsuro</creatorcontrib><creatorcontrib>Tono, Kayoko</creatorcontrib><creatorcontrib>Uchiyama, Yoshiyasu</creatorcontrib><creatorcontrib>Okada, Yoshinori</creatorcontrib><creatorcontrib>Masuda, Maki</creatorcontrib><creatorcontrib>Soeda, Shuichi</creatorcontrib><creatorcontrib>Nitta, Masahiro</creatorcontrib><creatorcontrib>Akatsuka, Akira</creatorcontrib><title>Origin and hierarchy of basal lamina-forming and -non-forming myogenic cells in mouse skeletal muscle in relation to adhesive capacity and Pax7 expression in vitro</title><title>Cell and tissue research</title><addtitle>Cell Tissue Res</addtitle><addtitle>Cell Tissue Res</addtitle><description>As a novel approach to distinguish skeletal myogenic cell populations, basal lamina (BL) formation of myogenic cells was examined in the mouse compensatory enlarged plantaris muscles in vivo and in fiber-bundle cultures in vitro. MyoD
+
myogenic cells located inside the regenerative muscle fiber BL were laminin
−
but interstitial MyoD
+
cells were laminin
+
. This was also confirmed by electron microscopy as structural BL formation. Similar trends were observed in the fiber-bundle cultures including satellite cells and interstitial myogenic cells and laminin
+
myogenic cells predominantly showed non-adhesive (non-Ad) behavior with Pax7
−
, whereas laminin
−
cells were adhesive (Ad) with Pax7
+
. Moreover, non-Ad/laminin
+
and Ad/laminin
−
myotubes were also observed and the former type showed spontaneous contractions, while the latter type did not. The origin and hierarchy of Ad/Pax7
+
/laminin
−
and non-Ad/Pax7
−
/laminin
+
myogenic cells were also examined using skeletal muscle interstitium-derived CD34
+
/45
−
(Sk-34) and CD34
−
/45
−
(Sk-DN) multipotent stem cells, which were composed of non-committed myogenic cells with a few (<1%) Pax7
+
cells in the Sk-DN cells at fresh isolation. Both cell types were separated by Ad/non-Ad capacity in repetitive culture. As expected, both Ad/Pax7
+
/laminin
−
and non-Ad/Pax7
−
/laminin
+
myogenic cells consistently appeared in the Ad and non-Ad cell culture. However, Ad/Pax7
+
/laminin
−
cells were repeatedly detected in the non-Ad cell culture, while the opposite phenomenon did not occur. This indicates that the source of non-Ad/ Pax7
−
/laminin
+
myogenic cells was present in the Sk-34 and Sk-DN stem cells and they were able to produce Ad/ Pax7
+
/ laminin
−
myogenic cells during myogenesis as primary myoblasts and situated hierarchically upstream of the latter cells.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Adhesion</subject><subject>Cell adhesion & migration</subject><subject>Cell Line</subject><subject>Cells, Cultured</subject><subject>Desmin - analysis</subject><subject>Desmin - genetics</subject><subject>Electron microscopy</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Human Genetics</subject><subject>Integrin beta1 - genetics</subject><subject>Laminin - analysis</subject><subject>Laminin - genetics</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Molecular Medicine</subject><subject>Muscle Development</subject><subject>Muscle Fibers, Skeletal - cytology</subject><subject>Muscle Fibers, Skeletal - metabolism</subject><subject>Muscle, Skeletal - cytology</subject><subject>Muscle, Skeletal - ultrastructure</subject><subject>Muscles</subject><subject>Musculoskeletal system</subject><subject>MyoD Protein - analysis</subject><subject>MyoD Protein - genetics</subject><subject>Myogenin - analysis</subject><subject>Myogenin - genetics</subject><subject>PAX7 Transcription Factor - analysis</subject><subject>PAX7 Transcription Factor - genetics</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Regular Article</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Rodents</subject><issn>0302-766X</issn><issn>1432-0878</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1ks9u1DAQxi0EosvCA3BBFpXglGI7Tpw9VhX_pErlABK3aOJMEhfHXuyk6j4PL4rTlEIRyIeRZ37fJ894CHnO2QlnTL2JjEnJM8ZZxrlQ2e4B2XCZi4xVqnpINixnIlNl-fWIPInxkjEuy3L3mByJRMuiVBvy4yKY3jgKrqWDwQBBDwfqO9pABEstjMZB1vmQYn9DZc67u8R48D06o6lGayNNRqOfI9L4DS1OyWCco7a4FAJamIx3dPIU2gGjuUKqYQ_aTIcb509wrShe7wPGuIBJdGWm4J-SRx3YiM9u45Z8eff289mH7Pzi_cez0_NMy7KasoZJLHSLed42upWKIaquKJiWouEgFaJooGjzRgDnTduUWlYSmC4Uykpjm2_J69V3H_z3GeNUjyYujYHD1FVdFWmsuShZIl_-RV76Obj0uAUSSnBWJeh4hXqwWBvX-SmAXizr07yQVbHL02dtyck_qHRaHI32DjuT8vcEr_4QDAh2GqK38zLbeB_kK6iDjzFgV--DGSEcas7qZX_qdX9qttzTRtS7pHlx29jcjNjeKX4tTALECsRUcj2G353_3_Unzc3RWg</recordid><startdate>20110401</startdate><enddate>20110401</enddate><creator>Tamaki, Tetsuro</creator><creator>Tono, Kayoko</creator><creator>Uchiyama, Yoshiyasu</creator><creator>Okada, Yoshinori</creator><creator>Masuda, Maki</creator><creator>Soeda, Shuichi</creator><creator>Nitta, Masahiro</creator><creator>Akatsuka, Akira</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7RV</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20110401</creationdate><title>Origin and hierarchy of basal lamina-forming and -non-forming myogenic cells in mouse skeletal muscle in relation to adhesive capacity and Pax7 expression in vitro</title><author>Tamaki, Tetsuro ; Tono, Kayoko ; Uchiyama, Yoshiyasu ; Okada, Yoshinori ; Masuda, Maki ; Soeda, Shuichi ; Nitta, Masahiro ; Akatsuka, Akira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-b04e5cde33dbcd470ee7f550c42b1a47ee2ba5d3b2a11bdb6c484a0c57e48ced3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Adhesion</topic><topic>Cell adhesion & migration</topic><topic>Cell Line</topic><topic>Cells, Cultured</topic><topic>Desmin - analysis</topic><topic>Desmin - genetics</topic><topic>Electron microscopy</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Human Genetics</topic><topic>Integrin beta1 - genetics</topic><topic>Laminin - analysis</topic><topic>Laminin - genetics</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Molecular Medicine</topic><topic>Muscle Development</topic><topic>Muscle Fibers, Skeletal - cytology</topic><topic>Muscle Fibers, Skeletal - metabolism</topic><topic>Muscle, Skeletal - cytology</topic><topic>Muscle, Skeletal - ultrastructure</topic><topic>Muscles</topic><topic>Musculoskeletal system</topic><topic>MyoD Protein - analysis</topic><topic>MyoD Protein - genetics</topic><topic>Myogenin - analysis</topic><topic>Myogenin - genetics</topic><topic>PAX7 Transcription Factor - analysis</topic><topic>PAX7 Transcription Factor - genetics</topic><topic>Proteins</topic><topic>Proteomics</topic><topic>Regular Article</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Rodents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tamaki, Tetsuro</creatorcontrib><creatorcontrib>Tono, Kayoko</creatorcontrib><creatorcontrib>Uchiyama, Yoshiyasu</creatorcontrib><creatorcontrib>Okada, Yoshinori</creatorcontrib><creatorcontrib>Masuda, Maki</creatorcontrib><creatorcontrib>Soeda, Shuichi</creatorcontrib><creatorcontrib>Nitta, Masahiro</creatorcontrib><creatorcontrib>Akatsuka, Akira</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell and tissue research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tamaki, Tetsuro</au><au>Tono, Kayoko</au><au>Uchiyama, Yoshiyasu</au><au>Okada, Yoshinori</au><au>Masuda, Maki</au><au>Soeda, Shuichi</au><au>Nitta, Masahiro</au><au>Akatsuka, Akira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Origin and hierarchy of basal lamina-forming and -non-forming myogenic cells in mouse skeletal muscle in relation to adhesive capacity and Pax7 expression in vitro</atitle><jtitle>Cell and tissue research</jtitle><stitle>Cell Tissue Res</stitle><addtitle>Cell Tissue Res</addtitle><date>2011-04-01</date><risdate>2011</risdate><volume>344</volume><issue>1</issue><spage>147</spage><epage>168</epage><pages>147-168</pages><issn>0302-766X</issn><eissn>1432-0878</eissn><abstract>As a novel approach to distinguish skeletal myogenic cell populations, basal lamina (BL) formation of myogenic cells was examined in the mouse compensatory enlarged plantaris muscles in vivo and in fiber-bundle cultures in vitro. MyoD
+
myogenic cells located inside the regenerative muscle fiber BL were laminin
−
but interstitial MyoD
+
cells were laminin
+
. This was also confirmed by electron microscopy as structural BL formation. Similar trends were observed in the fiber-bundle cultures including satellite cells and interstitial myogenic cells and laminin
+
myogenic cells predominantly showed non-adhesive (non-Ad) behavior with Pax7
−
, whereas laminin
−
cells were adhesive (Ad) with Pax7
+
. Moreover, non-Ad/laminin
+
and Ad/laminin
−
myotubes were also observed and the former type showed spontaneous contractions, while the latter type did not. The origin and hierarchy of Ad/Pax7
+
/laminin
−
and non-Ad/Pax7
−
/laminin
+
myogenic cells were also examined using skeletal muscle interstitium-derived CD34
+
/45
−
(Sk-34) and CD34
−
/45
−
(Sk-DN) multipotent stem cells, which were composed of non-committed myogenic cells with a few (<1%) Pax7
+
cells in the Sk-DN cells at fresh isolation. Both cell types were separated by Ad/non-Ad capacity in repetitive culture. As expected, both Ad/Pax7
+
/laminin
−
and non-Ad/Pax7
−
/laminin
+
myogenic cells consistently appeared in the Ad and non-Ad cell culture. However, Ad/Pax7
+
/laminin
−
cells were repeatedly detected in the non-Ad cell culture, while the opposite phenomenon did not occur. This indicates that the source of non-Ad/ Pax7
−
/laminin
+
myogenic cells was present in the Sk-34 and Sk-DN stem cells and they were able to produce Ad/ Pax7
+
/ laminin
−
myogenic cells during myogenesis as primary myoblasts and situated hierarchically upstream of the latter cells.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>21274567</pmid><doi>10.1007/s00441-010-1127-9</doi><tpages>22</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0302-766X |
| ispartof | Cell and tissue research, 2011-04, Vol.344 (1), p.147-168 |
| issn | 0302-766X 1432-0878 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_858783260 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animals Biomedical and Life Sciences Biomedicine Cell Adhesion Cell adhesion & migration Cell Line Cells, Cultured Desmin - analysis Desmin - genetics Electron microscopy Gene expression Gene Expression Regulation, Developmental Human Genetics Integrin beta1 - genetics Laminin - analysis Laminin - genetics Mice Mice, Inbred C57BL Molecular Medicine Muscle Development Muscle Fibers, Skeletal - cytology Muscle Fibers, Skeletal - metabolism Muscle, Skeletal - cytology Muscle, Skeletal - ultrastructure Muscles Musculoskeletal system MyoD Protein - analysis MyoD Protein - genetics Myogenin - analysis Myogenin - genetics PAX7 Transcription Factor - analysis PAX7 Transcription Factor - genetics Proteins Proteomics Regular Article Reverse Transcriptase Polymerase Chain Reaction Rodents |
| title | Origin and hierarchy of basal lamina-forming and -non-forming myogenic cells in mouse skeletal muscle in relation to adhesive capacity and Pax7 expression in vitro |
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