Improved analytical precision of 1,4-dihydroxy-2-naphthoic acid by high performance liquid chromatography using dithiothreitol as mobile phase additive
1,4-dihydroxyl-2-naphtoic acid (DHNA) is a bifidogenic growth stimulator from Propionibacterium freudenreichii. According to a method described earlier, the peak area of DHNA measured by a high-performance liquid chromatography (HPLC) gradually increases with repeated analyses of the same sample. We...
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| Veröffentlicht in: | Food Science and Technology Research 2008, Vol.14(5), pp.509-509 |
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| creator | Takebayashi, Jun Nagata, Junichi Yamada, Kazuhiko |
| description | 1,4-dihydroxyl-2-naphtoic acid (DHNA) is a bifidogenic growth stimulator from Propionibacterium freudenreichii. According to a method described earlier, the peak area of DHNA measured by a high-performance liquid chromatography (HPLC) gradually increases with repeated analyses of the same sample. We hypothesized that the oxidizability of DHNA was the cause of poor precision. Therefore, we attempted to develop an improved method using dl-dithiothreitol (DTT) as a mobile phase additive. A DHNA standard solution (5 µg/ml) was analyzed five times in a row by either the original or the improved method. The relative standard deviation (R.S.D.) of the peak area was 37.0% and 1.6%, respectively. The linearity of the improved method was confirmed in the range of 0.25-10 µg/ml (R2 = 0.9998). These data indicate that the addition of DTT to the mobile phase improves precision of the analysis of DHNA by HPLC. |
| doi_str_mv | 10.3136/fstr.14.509 |
| format | Article |
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According to a method described earlier, the peak area of DHNA measured by a high-performance liquid chromatography (HPLC) gradually increases with repeated analyses of the same sample. We hypothesized that the oxidizability of DHNA was the cause of poor precision. Therefore, we attempted to develop an improved method using dl-dithiothreitol (DTT) as a mobile phase additive. A DHNA standard solution (5 µg/ml) was analyzed five times in a row by either the original or the improved method. The relative standard deviation (R.S.D.) of the peak area was 37.0% and 1.6%, respectively. The linearity of the improved method was confirmed in the range of 0.25-10 µg/ml (R2 = 0.9998). These data indicate that the addition of DTT to the mobile phase improves precision of the analysis of DHNA by HPLC.</description><identifier>ISSN: 1344-6606</identifier><identifier>ISSN: 1881-3984</identifier><identifier>EISSN: 1881-3984</identifier><identifier>DOI: 10.3136/fstr.14.509</identifier><language>eng</language><publisher>Tsukuba: Japanese Society for Food Science and Technology</publisher><subject>1,4-dihydroxyl-2-naphtoic acid (DHNA) ; accuracy ; additives ; analytical methods ; bifidogenic growth stimulators ; dithiothreitol ; dithiothreitol (DTT) ; high performance liquid chromatography ; HPLC analysis ; methodology ; mobile phase additive ; monocarboxylic acids ; new methods ; oxidative stability ; prebiotics ; Propionibacterium freudenreichii ; quantitative analysis</subject><ispartof>Food Science and Technology Research, 2008, Vol.14(5), pp.509-509</ispartof><rights>2008 by Japanese Society for Food Science and Technology</rights><rights>Copyright Japan Science and Technology Agency 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c578t-c30878fbcb4b2eea1a2f8c8b9b0ef25a4734007f1139e1b30a85eb499c4793713</citedby><cites>FETCH-LOGICAL-c578t-c30878fbcb4b2eea1a2f8c8b9b0ef25a4734007f1139e1b30a85eb499c4793713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids></links><search><creatorcontrib>Takebayashi, Jun</creatorcontrib><creatorcontrib>Nagata, Junichi</creatorcontrib><creatorcontrib>Yamada, Kazuhiko</creatorcontrib><title>Improved analytical precision of 1,4-dihydroxy-2-naphthoic acid by high performance liquid chromatography using dithiothreitol as mobile phase additive</title><title>Food Science and Technology Research</title><addtitle>Food Science and Technology Research</addtitle><description>1,4-dihydroxyl-2-naphtoic acid (DHNA) is a bifidogenic growth stimulator from Propionibacterium freudenreichii. According to a method described earlier, the peak area of DHNA measured by a high-performance liquid chromatography (HPLC) gradually increases with repeated analyses of the same sample. We hypothesized that the oxidizability of DHNA was the cause of poor precision. Therefore, we attempted to develop an improved method using dl-dithiothreitol (DTT) as a mobile phase additive. A DHNA standard solution (5 µg/ml) was analyzed five times in a row by either the original or the improved method. The relative standard deviation (R.S.D.) of the peak area was 37.0% and 1.6%, respectively. The linearity of the improved method was confirmed in the range of 0.25-10 µg/ml (R2 = 0.9998). These data indicate that the addition of DTT to the mobile phase improves precision of the analysis of DHNA by HPLC.</description><subject>1,4-dihydroxyl-2-naphtoic acid (DHNA)</subject><subject>accuracy</subject><subject>additives</subject><subject>analytical methods</subject><subject>bifidogenic growth stimulators</subject><subject>dithiothreitol</subject><subject>dithiothreitol (DTT)</subject><subject>high performance liquid chromatography</subject><subject>HPLC analysis</subject><subject>methodology</subject><subject>mobile phase additive</subject><subject>monocarboxylic acids</subject><subject>new methods</subject><subject>oxidative stability</subject><subject>prebiotics</subject><subject>Propionibacterium freudenreichii</subject><subject>quantitative analysis</subject><issn>1344-6606</issn><issn>1881-3984</issn><issn>1881-3984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNpdkU2L1TAUhosoOI6u_AEGXLjQXpMmbZOdw-DHwIALnXU4TU9ucmmbTtIO9pf4d82lMoKbJHCe94WcpyheM3rgjDcfbVrigYlDTdWT4oJJyUqupHia31yIsmlo87x4kdKJUlYrWV0Uv2_GOYYH7AlMMGyLNzCQOaLxyYeJBEvYB1H23m19DL-2sionmN3igjcEjO9JtxHnj47MGG2II0wGyeDv1zwyLoYRlnCMObKRNfnpSHq_OB8WF9EvYSCQyBg6PyCZHSQk0GfAP-DL4pmFIeGrv_dlcffl88_rb-Xt968311e3palbuZSGU9lK25lOdBUiMKisNLJTHUVb1SBaLihtLWNcIes4BVljJ5QyolW8ZfyyeLf35i3cr5gWPfpkcBhgwrAmLWspKi7VmXz7H3kKa8xLS5qJhjeqrdiZer9TJoaUIlo9Rz9C3DSj-uxInx3lhM6OMv1pp09pgSM-shCziAH_sfuRI48j4yBqnHLFm73CQtBwjD7pux8VZTwbbvLXJf8Dh9uoog</recordid><startdate>2008</startdate><enddate>2008</enddate><creator>Takebayashi, Jun</creator><creator>Nagata, Junichi</creator><creator>Yamada, Kazuhiko</creator><general>Japanese Society for Food Science and Technology</general><general>Japan Science and Technology Agency</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QR</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7QH</scope><scope>7UA</scope></search><sort><creationdate>2008</creationdate><title>Improved analytical precision of 1,4-dihydroxy-2-naphthoic acid by high performance liquid chromatography using dithiothreitol as mobile phase additive</title><author>Takebayashi, Jun ; Nagata, Junichi ; Yamada, Kazuhiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c578t-c30878fbcb4b2eea1a2f8c8b9b0ef25a4734007f1139e1b30a85eb499c4793713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>1,4-dihydroxyl-2-naphtoic acid (DHNA)</topic><topic>accuracy</topic><topic>additives</topic><topic>analytical methods</topic><topic>bifidogenic growth stimulators</topic><topic>dithiothreitol</topic><topic>dithiothreitol (DTT)</topic><topic>high performance liquid chromatography</topic><topic>HPLC analysis</topic><topic>methodology</topic><topic>mobile phase additive</topic><topic>monocarboxylic acids</topic><topic>new methods</topic><topic>oxidative stability</topic><topic>prebiotics</topic><topic>Propionibacterium freudenreichii</topic><topic>quantitative analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takebayashi, Jun</creatorcontrib><creatorcontrib>Nagata, Junichi</creatorcontrib><creatorcontrib>Yamada, Kazuhiko</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Aqualine</collection><collection>Water Resources Abstracts</collection><jtitle>Food Science and Technology Research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takebayashi, Jun</au><au>Nagata, Junichi</au><au>Yamada, Kazuhiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved analytical precision of 1,4-dihydroxy-2-naphthoic acid by high performance liquid chromatography using dithiothreitol as mobile phase additive</atitle><jtitle>Food Science and Technology Research</jtitle><addtitle>Food Science and Technology Research</addtitle><date>2008</date><risdate>2008</risdate><volume>14</volume><issue>5</issue><spage>509</spage><epage>512</epage><pages>509-512</pages><issn>1344-6606</issn><issn>1881-3984</issn><eissn>1881-3984</eissn><abstract>1,4-dihydroxyl-2-naphtoic acid (DHNA) is a bifidogenic growth stimulator from Propionibacterium freudenreichii. According to a method described earlier, the peak area of DHNA measured by a high-performance liquid chromatography (HPLC) gradually increases with repeated analyses of the same sample. We hypothesized that the oxidizability of DHNA was the cause of poor precision. Therefore, we attempted to develop an improved method using dl-dithiothreitol (DTT) as a mobile phase additive. A DHNA standard solution (5 µg/ml) was analyzed five times in a row by either the original or the improved method. The relative standard deviation (R.S.D.) of the peak area was 37.0% and 1.6%, respectively. The linearity of the improved method was confirmed in the range of 0.25-10 µg/ml (R2 = 0.9998). These data indicate that the addition of DTT to the mobile phase improves precision of the analysis of DHNA by HPLC.</abstract><cop>Tsukuba</cop><pub>Japanese Society for Food Science and Technology</pub><doi>10.3136/fstr.14.509</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 1,4-dihydroxyl-2-naphtoic acid (DHNA) accuracy additives analytical methods bifidogenic growth stimulators dithiothreitol dithiothreitol (DTT) high performance liquid chromatography HPLC analysis methodology mobile phase additive monocarboxylic acids new methods oxidative stability prebiotics Propionibacterium freudenreichii quantitative analysis |
| title | Improved analytical precision of 1,4-dihydroxy-2-naphthoic acid by high performance liquid chromatography using dithiothreitol as mobile phase additive |
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