The Complexity of Human Leukocyte Antigen (HLA)-DQ Antibodies and Its Effect on Virtual Crossmatching
We previously reported that in patients possessing human leukocyte antigen (HLA)-DQ-directed antibodies, the target molecule may include the patient's own DQβ chain if it is paired with non-self-DQα chain, thus forming a different DQ target. Herein, we sought to assess the breadth of this pheno...
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| Veröffentlicht in: | Transplantation 2010-11, Vol.90 (10), p.1117-1124 |
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| creator | TAMBUR, Anat R LEVENTHAL, Joseph R FRIEDEWALD, John J RAMON, Daniel S |
| description | We previously reported that in patients possessing human leukocyte antigen (HLA)-DQ-directed antibodies, the target molecule may include the patient's own DQβ chain if it is paired with non-self-DQα chain, thus forming a different DQ target. Herein, we sought to assess the breadth of this phenomenon.
Serum samples from 104 patients awaiting kidney transplantation, known to have DQ antibodies, were studied. Antibody identification was performed using luminex-based HLA class II single-antigen bead assays from two vendors; DQA1/DQB1 typing was performed using luminex polymerase chain reaction - sequence specific oligo prob hybridization (PCR-SSO) technology.
A total of 71% of the 104 serum samples studied contained antibodies reactive against test beads coated with the patient's own DQα- or β-chain components. Of those, 35 patients (34%) exhibited antibodies to their own DQβ chain when in combination with non-self-DQα chains; and 64 patients (62%) had antibodies to their own DQα chain when in combination with non-self-DQβ chains. This is a striking observation.
To the best of our knowledge, this is the first systematic, high-resolution evaluation of DQ antibody repertoire. With the expansion of virtual crossmatching, particularly in the context of a national registry, the need for more detailed DQ antibody or antigen evaluation is critical to improve operational efficiency and patient outcomes. |
| doi_str_mv | 10.1097/tp.0b013e3181f89c6d |
| format | Article |
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Serum samples from 104 patients awaiting kidney transplantation, known to have DQ antibodies, were studied. Antibody identification was performed using luminex-based HLA class II single-antigen bead assays from two vendors; DQA1/DQB1 typing was performed using luminex polymerase chain reaction - sequence specific oligo prob hybridization (PCR-SSO) technology.
A total of 71% of the 104 serum samples studied contained antibodies reactive against test beads coated with the patient's own DQα- or β-chain components. Of those, 35 patients (34%) exhibited antibodies to their own DQβ chain when in combination with non-self-DQα chains; and 64 patients (62%) had antibodies to their own DQα chain when in combination with non-self-DQβ chains. This is a striking observation.
To the best of our knowledge, this is the first systematic, high-resolution evaluation of DQ antibody repertoire. With the expansion of virtual crossmatching, particularly in the context of a national registry, the need for more detailed DQ antibody or antigen evaluation is critical to improve operational efficiency and patient outcomes.</description><identifier>ISSN: 0041-1337</identifier><identifier>EISSN: 1534-6080</identifier><identifier>DOI: 10.1097/tp.0b013e3181f89c6d</identifier><identifier>PMID: 20847715</identifier><identifier>CODEN: TRPLAU</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins</publisher><subject>Adult ; Aged ; Alleles ; Autoantibodies - immunology ; Autoantigens - chemistry ; Autoantigens - genetics ; Autoantigens - immunology ; Biological and medical sciences ; Epitopes - chemistry ; Epitopes - genetics ; Epitopes - immunology ; Female ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Histocompatibility Testing ; HLA-DQ alpha-Chains ; HLA-DQ Antigens - chemistry ; HLA-DQ Antigens - genetics ; HLA-DQ Antigens - immunology ; HLA-DQ beta-Chains ; Humans ; Isoantibodies - immunology ; Kidney Transplantation - immunology ; Linkage Disequilibrium ; Male ; Medical sciences ; Middle Aged ; Models, Molecular ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Tissue, organ and graft immunology ; User-Computer Interface ; Young Adult</subject><ispartof>Transplantation, 2010-11, Vol.90 (10), p.1117-1124</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c432t-a7f92d6cf7c559eb7f1ec41420972926df4433683b9b46df3e862c3deeebf0c53</citedby><cites>FETCH-LOGICAL-c432t-a7f92d6cf7c559eb7f1ec41420972926df4433683b9b46df3e862c3deeebf0c53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23503855$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20847715$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TAMBUR, Anat R</creatorcontrib><creatorcontrib>LEVENTHAL, Joseph R</creatorcontrib><creatorcontrib>FRIEDEWALD, John J</creatorcontrib><creatorcontrib>RAMON, Daniel S</creatorcontrib><title>The Complexity of Human Leukocyte Antigen (HLA)-DQ Antibodies and Its Effect on Virtual Crossmatching</title><title>Transplantation</title><addtitle>Transplantation</addtitle><description>We previously reported that in patients possessing human leukocyte antigen (HLA)-DQ-directed antibodies, the target molecule may include the patient's own DQβ chain if it is paired with non-self-DQα chain, thus forming a different DQ target. Herein, we sought to assess the breadth of this phenomenon.
Serum samples from 104 patients awaiting kidney transplantation, known to have DQ antibodies, were studied. Antibody identification was performed using luminex-based HLA class II single-antigen bead assays from two vendors; DQA1/DQB1 typing was performed using luminex polymerase chain reaction - sequence specific oligo prob hybridization (PCR-SSO) technology.
A total of 71% of the 104 serum samples studied contained antibodies reactive against test beads coated with the patient's own DQα- or β-chain components. Of those, 35 patients (34%) exhibited antibodies to their own DQβ chain when in combination with non-self-DQα chains; and 64 patients (62%) had antibodies to their own DQα chain when in combination with non-self-DQβ chains. This is a striking observation.
To the best of our knowledge, this is the first systematic, high-resolution evaluation of DQ antibody repertoire. With the expansion of virtual crossmatching, particularly in the context of a national registry, the need for more detailed DQ antibody or antigen evaluation is critical to improve operational efficiency and patient outcomes.</description><subject>Adult</subject><subject>Aged</subject><subject>Alleles</subject><subject>Autoantibodies - immunology</subject><subject>Autoantigens - chemistry</subject><subject>Autoantigens - genetics</subject><subject>Autoantigens - immunology</subject><subject>Biological and medical sciences</subject><subject>Epitopes - chemistry</subject><subject>Epitopes - genetics</subject><subject>Epitopes - immunology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Histocompatibility Testing</subject><subject>HLA-DQ alpha-Chains</subject><subject>HLA-DQ Antigens - chemistry</subject><subject>HLA-DQ Antigens - genetics</subject><subject>HLA-DQ Antigens - immunology</subject><subject>HLA-DQ beta-Chains</subject><subject>Humans</subject><subject>Isoantibodies - immunology</subject><subject>Kidney Transplantation - immunology</subject><subject>Linkage Disequilibrium</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Models, Molecular</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Tissue, organ and graft immunology</subject><subject>User-Computer Interface</subject><subject>Young Adult</subject><issn>0041-1337</issn><issn>1534-6080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkVtLxDAQhYMoul5-gSB5EfWhmnSSJn1c1ssKCyqsvpY0nWi1l7VJwf33Vl0VfPJpmOE7B84cQvY5O-UsVWdhccpyxgGBa-50apNijYy4BBElTLN1MmJM8IgDqC2y7f0zY0yCUptkK2ZaKMXliOD8CemkrRcVvpVhSVtHp31tGjrD_qW1y4B03ITyERt6PJ2NT6Lzu89D3hYlemqagl4HTy-cQxto29CHsgu9qeika72vTbBPZfO4SzacqTzureYOub-8mE-m0ezm6noynkVWQBwio1waF4l1ykqZYq4cRyu4iIe4cRonhRMCINGQp7kYNkCdxBYKRMwdsxJ2yNGX76JrX3v0IatLb7GqTINt7zMtteBKg_gHmSjNRMwHEr5I-5GoQ5cturI23TLjLPsoIpvfZn-LGFQHK_8-r7H40Xx_fgAOV4Dx1lSuM40t_S8HkoGWEt4BVZyRdg</recordid><startdate>20101127</startdate><enddate>20101127</enddate><creator>TAMBUR, Anat R</creator><creator>LEVENTHAL, Joseph R</creator><creator>FRIEDEWALD, John J</creator><creator>RAMON, Daniel S</creator><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20101127</creationdate><title>The Complexity of Human Leukocyte Antigen (HLA)-DQ Antibodies and Its Effect on Virtual Crossmatching</title><author>TAMBUR, Anat R ; LEVENTHAL, Joseph R ; FRIEDEWALD, John J ; RAMON, Daniel S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-a7f92d6cf7c559eb7f1ec41420972926df4433683b9b46df3e862c3deeebf0c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Alleles</topic><topic>Autoantibodies - immunology</topic><topic>Autoantigens - chemistry</topic><topic>Autoantigens - genetics</topic><topic>Autoantigens - immunology</topic><topic>Biological and medical sciences</topic><topic>Epitopes - chemistry</topic><topic>Epitopes - genetics</topic><topic>Epitopes - immunology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Histocompatibility Testing</topic><topic>HLA-DQ alpha-Chains</topic><topic>HLA-DQ Antigens - chemistry</topic><topic>HLA-DQ Antigens - genetics</topic><topic>HLA-DQ Antigens - immunology</topic><topic>HLA-DQ beta-Chains</topic><topic>Humans</topic><topic>Isoantibodies - immunology</topic><topic>Kidney Transplantation - immunology</topic><topic>Linkage Disequilibrium</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Models, Molecular</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Tissue, organ and graft immunology</topic><topic>User-Computer Interface</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TAMBUR, Anat R</creatorcontrib><creatorcontrib>LEVENTHAL, Joseph R</creatorcontrib><creatorcontrib>FRIEDEWALD, John J</creatorcontrib><creatorcontrib>RAMON, Daniel S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transplantation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TAMBUR, Anat R</au><au>LEVENTHAL, Joseph R</au><au>FRIEDEWALD, John J</au><au>RAMON, Daniel S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Complexity of Human Leukocyte Antigen (HLA)-DQ Antibodies and Its Effect on Virtual Crossmatching</atitle><jtitle>Transplantation</jtitle><addtitle>Transplantation</addtitle><date>2010-11-27</date><risdate>2010</risdate><volume>90</volume><issue>10</issue><spage>1117</spage><epage>1124</epage><pages>1117-1124</pages><issn>0041-1337</issn><eissn>1534-6080</eissn><coden>TRPLAU</coden><abstract>We previously reported that in patients possessing human leukocyte antigen (HLA)-DQ-directed antibodies, the target molecule may include the patient's own DQβ chain if it is paired with non-self-DQα chain, thus forming a different DQ target. Herein, we sought to assess the breadth of this phenomenon.
Serum samples from 104 patients awaiting kidney transplantation, known to have DQ antibodies, were studied. Antibody identification was performed using luminex-based HLA class II single-antigen bead assays from two vendors; DQA1/DQB1 typing was performed using luminex polymerase chain reaction - sequence specific oligo prob hybridization (PCR-SSO) technology.
A total of 71% of the 104 serum samples studied contained antibodies reactive against test beads coated with the patient's own DQα- or β-chain components. Of those, 35 patients (34%) exhibited antibodies to their own DQβ chain when in combination with non-self-DQα chains; and 64 patients (62%) had antibodies to their own DQα chain when in combination with non-self-DQβ chains. This is a striking observation.
To the best of our knowledge, this is the first systematic, high-resolution evaluation of DQ antibody repertoire. With the expansion of virtual crossmatching, particularly in the context of a national registry, the need for more detailed DQ antibody or antigen evaluation is critical to improve operational efficiency and patient outcomes.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins</pub><pmid>20847715</pmid><doi>10.1097/tp.0b013e3181f89c6d</doi><tpages>8</tpages></addata></record> |
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| subjects | Adult Aged Alleles Autoantibodies - immunology Autoantigens - chemistry Autoantigens - genetics Autoantigens - immunology Biological and medical sciences Epitopes - chemistry Epitopes - genetics Epitopes - immunology Female Fundamental and applied biological sciences. Psychology Fundamental immunology Histocompatibility Testing HLA-DQ alpha-Chains HLA-DQ Antigens - chemistry HLA-DQ Antigens - genetics HLA-DQ Antigens - immunology HLA-DQ beta-Chains Humans Isoantibodies - immunology Kidney Transplantation - immunology Linkage Disequilibrium Male Medical sciences Middle Aged Models, Molecular Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Tissue, organ and graft immunology User-Computer Interface Young Adult |
| title | The Complexity of Human Leukocyte Antigen (HLA)-DQ Antibodies and Its Effect on Virtual Crossmatching |
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