Transcription factor osterix modified bone marrow mesenchymal stem cells enhance callus formation during distraction osteogenesis
This study was designed to investigate the effects of local delivery of bone marrow mesenchymal stem cells (BMMSCs) with or without osterix (OSX) gene transfected on bone regeneration in the distracted zone using a rabbit model of mandibular lengthening. Fifty-four New Zealand white rabbits underwen...
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| Veröffentlicht in: | Oral surgery, oral medicine, oral pathology, oral radiology and endodontics oral medicine, oral pathology, oral radiology and endodontics, 2011-04, Vol.111 (4), p.412-419 |
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| creator | Lai, Qing-Guo, DMD Yuan, Kui-Feng, DMD Xu, Xin, PhD Li, De-ren, MD Li, Guo-Ju, DMD Wei, Fu-Lan, PhD Yang, Zhong-Jun, DMD Luo, Sheng-Lei, DMD Tang, Xiao-Peng, BS Li, Shu, PhD |
| description | This study was designed to investigate the effects of local delivery of bone marrow mesenchymal stem cells (BMMSCs) with or without osterix (OSX) gene transfected on bone regeneration in the distracted zone using a rabbit model of mandibular lengthening. Fifty-four New Zealand white rabbits underwent osteodistraction of the left mandible and were then randomly divided into group A, group B, and group C (n = 18 for each group). At the end of distraction BMMSCs transfected with OSX, autologous BMMSCs and physiological saline were injected into the distraction gaps in groups A, B, and C, respectively. Nine animals from each group were humanely killed at 2 and 6 weeks after completion of distraction. The distracted mandibles were harvested and processed for radiographic, histological, and immunohistochemical examination. Excellent bone formation in the distracted callus was observed in group A and group B; the former showed better bone formation and highest bone mineral density (BMD), thickness of new trabeculae (TNT, mm) and volumes of the newly formed bone area (NBV) in the distraction zones. Group C animals showed poor bone formation in the distracted callus when compared with groups A and B. Positive immunostaining of bone sialoprotein (BSP) was observed in the distracted callus in all groups; however, BSP expression was much stronger in group A than in groups B and C. The results of this study suggest transplantation of BMMSCs can promote bone formation in DO; OSX-mediated ex vivo gene therapy was more effective during bone deposition and callus formation in distraction osteogenesis. |
| doi_str_mv | 10.1016/j.tripleo.2010.05.012 |
| format | Article |
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Fifty-four New Zealand white rabbits underwent osteodistraction of the left mandible and were then randomly divided into group A, group B, and group C (n = 18 for each group). At the end of distraction BMMSCs transfected with OSX, autologous BMMSCs and physiological saline were injected into the distraction gaps in groups A, B, and C, respectively. Nine animals from each group were humanely killed at 2 and 6 weeks after completion of distraction. The distracted mandibles were harvested and processed for radiographic, histological, and immunohistochemical examination. Excellent bone formation in the distracted callus was observed in group A and group B; the former showed better bone formation and highest bone mineral density (BMD), thickness of new trabeculae (TNT, mm) and volumes of the newly formed bone area (NBV) in the distraction zones. Group C animals showed poor bone formation in the distracted callus when compared with groups A and B. Positive immunostaining of bone sialoprotein (BSP) was observed in the distracted callus in all groups; however, BSP expression was much stronger in group A than in groups B and C. The results of this study suggest transplantation of BMMSCs can promote bone formation in DO; OSX-mediated ex vivo gene therapy was more effective during bone deposition and callus formation in distraction osteogenesis.</description><identifier>ISSN: 1079-2104</identifier><identifier>EISSN: 1528-395X</identifier><identifier>DOI: 10.1016/j.tripleo.2010.05.012</identifier><identifier>PMID: 20813560</identifier><language>eng</language><publisher>New York, NY: Mosby, Inc</publisher><subject>2,4,6-Trinitrotoluene ; Animals ; Biological and medical sciences ; Bone Marrow Transplantation ; Bony Callus - growth & development ; Cell Culture Techniques ; Cell physiology ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental ; Integrin-Binding Sialoprotein - biosynthesis ; Male ; Mandible - surgery ; Medical sciences ; Mesenchymal Stem Cell Transplantation - methods ; Mineralization, calcification ; Molecular and cellular biology ; Osteoblasts - metabolism ; Osteogenesis - genetics ; Osteogenesis, Distraction - methods ; Otorhinolaryngology. Stomatology ; Rabbits ; Random Allocation ; Recombinant Proteins ; Skeleton and joints ; Sp7 Transcription Factor ; Surgery ; Transcription Factors - genetics ; Transcription Factors - physiology ; Transfection ; Vertebrates: osteoarticular system, musculoskeletal system ; Zinc Fingers - genetics</subject><ispartof>Oral surgery, oral medicine, oral pathology, oral radiology and endodontics, 2011-04, Vol.111 (4), p.412-419</ispartof><rights>Mosby, Inc.</rights><rights>2011 Mosby, Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Mosby, Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-b79d30f089131e4afe055b62b552e6367e545dbc0bc95f2126f2308c1591e3023</citedby><cites>FETCH-LOGICAL-c482t-b79d30f089131e4afe055b62b552e6367e545dbc0bc95f2126f2308c1591e3023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1079210410003240$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24060551$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20813560$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lai, Qing-Guo, DMD</creatorcontrib><creatorcontrib>Yuan, Kui-Feng, DMD</creatorcontrib><creatorcontrib>Xu, Xin, PhD</creatorcontrib><creatorcontrib>Li, De-ren, MD</creatorcontrib><creatorcontrib>Li, Guo-Ju, DMD</creatorcontrib><creatorcontrib>Wei, Fu-Lan, PhD</creatorcontrib><creatorcontrib>Yang, Zhong-Jun, DMD</creatorcontrib><creatorcontrib>Luo, Sheng-Lei, DMD</creatorcontrib><creatorcontrib>Tang, Xiao-Peng, BS</creatorcontrib><creatorcontrib>Li, Shu, PhD</creatorcontrib><title>Transcription factor osterix modified bone marrow mesenchymal stem cells enhance callus formation during distraction osteogenesis</title><title>Oral surgery, oral medicine, oral pathology, oral radiology and endodontics</title><addtitle>Oral Surg Oral Med Oral Pathol Oral Radiol Endod</addtitle><description>This study was designed to investigate the effects of local delivery of bone marrow mesenchymal stem cells (BMMSCs) with or without osterix (OSX) gene transfected on bone regeneration in the distracted zone using a rabbit model of mandibular lengthening. Fifty-four New Zealand white rabbits underwent osteodistraction of the left mandible and were then randomly divided into group A, group B, and group C (n = 18 for each group). At the end of distraction BMMSCs transfected with OSX, autologous BMMSCs and physiological saline were injected into the distraction gaps in groups A, B, and C, respectively. Nine animals from each group were humanely killed at 2 and 6 weeks after completion of distraction. The distracted mandibles were harvested and processed for radiographic, histological, and immunohistochemical examination. Excellent bone formation in the distracted callus was observed in group A and group B; the former showed better bone formation and highest bone mineral density (BMD), thickness of new trabeculae (TNT, mm) and volumes of the newly formed bone area (NBV) in the distraction zones. Group C animals showed poor bone formation in the distracted callus when compared with groups A and B. Positive immunostaining of bone sialoprotein (BSP) was observed in the distracted callus in all groups; however, BSP expression was much stronger in group A than in groups B and C. The results of this study suggest transplantation of BMMSCs can promote bone formation in DO; OSX-mediated ex vivo gene therapy was more effective during bone deposition and callus formation in distraction osteogenesis.</description><subject>2,4,6-Trinitrotoluene</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bone Marrow Transplantation</subject><subject>Bony Callus - growth & development</subject><subject>Cell Culture Techniques</subject><subject>Cell physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Integrin-Binding Sialoprotein - biosynthesis</subject><subject>Male</subject><subject>Mandible - surgery</subject><subject>Medical sciences</subject><subject>Mesenchymal Stem Cell Transplantation - methods</subject><subject>Mineralization, calcification</subject><subject>Molecular and cellular biology</subject><subject>Osteoblasts - metabolism</subject><subject>Osteogenesis - genetics</subject><subject>Osteogenesis, Distraction - methods</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Rabbits</subject><subject>Random Allocation</subject><subject>Recombinant Proteins</subject><subject>Skeleton and joints</subject><subject>Sp7 Transcription Factor</subject><subject>Surgery</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - physiology</subject><subject>Transfection</subject><subject>Vertebrates: osteoarticular system, musculoskeletal system</subject><subject>Zinc Fingers - genetics</subject><issn>1079-2104</issn><issn>1528-395X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk9v1DAQxSMEoqXwEUC-ILhkGdvrbHKhQhX_pEocKBI3y3EmrZfEXjwJsEe-ORN2AYkDnGxZv3kzfm-K4qGElQRZPduuphx2A6aVAn4DswKpbhWn0qi61I35eJvvsGlKJWF9Utwj2gJApZvmbnGioJbaVHBafL_KLpJnqSmkKHrnp5RFoglz-CbG1IU-YCfaFFGMLuf0VYxIGP3NfnSDYG4UHoeBBMYbFz0K74ZhJtGnPLqfmt2cQ7wWXaAps_zytOina4xIge4Xd3o3ED44nmfFh1cvry7elJfvXr-9eHFZ-nWtprLdNJ2GHupGaolr1yMY01aqNUZhpasNmrXpWg-tb0yvpKp6paH20jQSNSh9Vjw56O5y-jwjTXYMtIzuIqaZbG1qVTdaLeTTf5Ls_6YxpjaGUXNAfU5EGXu7y4F92jO0cJXd2mNOdsnJgrGcE9c9OraY2xG731W_gmHg8RFwxI72nJIP9IdbQ8X_l8ydHzhk674EzJZ84HiwCxn9ZLsU_jvK878U_BBi4KafcI-0TXOOnIuVlpQF-35ZqmWnJK-T5jn0DzyTyvw</recordid><startdate>20110401</startdate><enddate>20110401</enddate><creator>Lai, Qing-Guo, DMD</creator><creator>Yuan, Kui-Feng, DMD</creator><creator>Xu, Xin, PhD</creator><creator>Li, De-ren, MD</creator><creator>Li, Guo-Ju, DMD</creator><creator>Wei, Fu-Lan, PhD</creator><creator>Yang, Zhong-Jun, DMD</creator><creator>Luo, Sheng-Lei, DMD</creator><creator>Tang, Xiao-Peng, BS</creator><creator>Li, Shu, PhD</creator><general>Mosby, Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20110401</creationdate><title>Transcription factor osterix modified bone marrow mesenchymal stem cells enhance callus formation during distraction osteogenesis</title><author>Lai, Qing-Guo, DMD ; Yuan, Kui-Feng, DMD ; Xu, Xin, PhD ; Li, De-ren, MD ; Li, Guo-Ju, DMD ; Wei, Fu-Lan, PhD ; Yang, Zhong-Jun, DMD ; Luo, Sheng-Lei, DMD ; Tang, Xiao-Peng, BS ; Li, Shu, PhD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-b79d30f089131e4afe055b62b552e6367e545dbc0bc95f2126f2308c1591e3023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>2,4,6-Trinitrotoluene</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bone Marrow Transplantation</topic><topic>Bony Callus - growth & development</topic><topic>Cell Culture Techniques</topic><topic>Cell physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Integrin-Binding Sialoprotein - biosynthesis</topic><topic>Male</topic><topic>Mandible - surgery</topic><topic>Medical sciences</topic><topic>Mesenchymal Stem Cell Transplantation - methods</topic><topic>Mineralization, calcification</topic><topic>Molecular and cellular biology</topic><topic>Osteoblasts - metabolism</topic><topic>Osteogenesis - genetics</topic><topic>Osteogenesis, Distraction - methods</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Rabbits</topic><topic>Random Allocation</topic><topic>Recombinant Proteins</topic><topic>Skeleton and joints</topic><topic>Sp7 Transcription Factor</topic><topic>Surgery</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - physiology</topic><topic>Transfection</topic><topic>Vertebrates: osteoarticular system, musculoskeletal system</topic><topic>Zinc Fingers - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lai, Qing-Guo, DMD</creatorcontrib><creatorcontrib>Yuan, Kui-Feng, DMD</creatorcontrib><creatorcontrib>Xu, Xin, PhD</creatorcontrib><creatorcontrib>Li, De-ren, MD</creatorcontrib><creatorcontrib>Li, Guo-Ju, DMD</creatorcontrib><creatorcontrib>Wei, Fu-Lan, PhD</creatorcontrib><creatorcontrib>Yang, Zhong-Jun, DMD</creatorcontrib><creatorcontrib>Luo, Sheng-Lei, DMD</creatorcontrib><creatorcontrib>Tang, Xiao-Peng, BS</creatorcontrib><creatorcontrib>Li, Shu, PhD</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Oral surgery, oral medicine, oral pathology, oral radiology and endodontics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lai, Qing-Guo, DMD</au><au>Yuan, Kui-Feng, DMD</au><au>Xu, Xin, PhD</au><au>Li, De-ren, MD</au><au>Li, Guo-Ju, DMD</au><au>Wei, Fu-Lan, PhD</au><au>Yang, Zhong-Jun, DMD</au><au>Luo, Sheng-Lei, DMD</au><au>Tang, Xiao-Peng, BS</au><au>Li, Shu, PhD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcription factor osterix modified bone marrow mesenchymal stem cells enhance callus formation during distraction osteogenesis</atitle><jtitle>Oral surgery, oral medicine, oral pathology, oral radiology and endodontics</jtitle><addtitle>Oral Surg Oral Med Oral Pathol Oral Radiol Endod</addtitle><date>2011-04-01</date><risdate>2011</risdate><volume>111</volume><issue>4</issue><spage>412</spage><epage>419</epage><pages>412-419</pages><issn>1079-2104</issn><eissn>1528-395X</eissn><abstract>This study was designed to investigate the effects of local delivery of bone marrow mesenchymal stem cells (BMMSCs) with or without osterix (OSX) gene transfected on bone regeneration in the distracted zone using a rabbit model of mandibular lengthening. Fifty-four New Zealand white rabbits underwent osteodistraction of the left mandible and were then randomly divided into group A, group B, and group C (n = 18 for each group). At the end of distraction BMMSCs transfected with OSX, autologous BMMSCs and physiological saline were injected into the distraction gaps in groups A, B, and C, respectively. Nine animals from each group were humanely killed at 2 and 6 weeks after completion of distraction. The distracted mandibles were harvested and processed for radiographic, histological, and immunohistochemical examination. Excellent bone formation in the distracted callus was observed in group A and group B; the former showed better bone formation and highest bone mineral density (BMD), thickness of new trabeculae (TNT, mm) and volumes of the newly formed bone area (NBV) in the distraction zones. Group C animals showed poor bone formation in the distracted callus when compared with groups A and B. Positive immunostaining of bone sialoprotein (BSP) was observed in the distracted callus in all groups; however, BSP expression was much stronger in group A than in groups B and C. The results of this study suggest transplantation of BMMSCs can promote bone formation in DO; OSX-mediated ex vivo gene therapy was more effective during bone deposition and callus formation in distraction osteogenesis.</abstract><cop>New York, NY</cop><pub>Mosby, Inc</pub><pmid>20813560</pmid><doi>10.1016/j.tripleo.2010.05.012</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1079-2104 |
| ispartof | Oral surgery, oral medicine, oral pathology, oral radiology and endodontics, 2011-04, Vol.111 (4), p.412-419 |
| issn | 1079-2104 1528-395X |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 2,4,6-Trinitrotoluene Animals Biological and medical sciences Bone Marrow Transplantation Bony Callus - growth & development Cell Culture Techniques Cell physiology Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental Integrin-Binding Sialoprotein - biosynthesis Male Mandible - surgery Medical sciences Mesenchymal Stem Cell Transplantation - methods Mineralization, calcification Molecular and cellular biology Osteoblasts - metabolism Osteogenesis - genetics Osteogenesis, Distraction - methods Otorhinolaryngology. Stomatology Rabbits Random Allocation Recombinant Proteins Skeleton and joints Sp7 Transcription Factor Surgery Transcription Factors - genetics Transcription Factors - physiology Transfection Vertebrates: osteoarticular system, musculoskeletal system Zinc Fingers - genetics |
| title | Transcription factor osterix modified bone marrow mesenchymal stem cells enhance callus formation during distraction osteogenesis |
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