Detection, verification and significance of differentially expressed miRNAs in bladder urothelial carcinoma
To explore the differentially expressed miRNAs in bladder urothelial carcinoma (BUC) in order to provide rationales for further studies of function and mechanism for their actions. Fresh normal (5 samples) and BUC (30 samples) tissues were collected from September 2008 to September 2009 in our hospi...
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| Veröffentlicht in: | Zhong hua yi xue za zhi 2010-12, Vol.90 (48), p.3391-3394 |
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| container_title | Zhong hua yi xue za zhi |
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| creator | Xie, Peng Xu, Feng Cheng, Wen Gao, Jian-Ping Zhang, Zheng-Yu Ge, Jing-Ping Wei, Zhi-Feng Xu, Xiao-Feng |
| description | To explore the differentially expressed miRNAs in bladder urothelial carcinoma (BUC) in order to provide rationales for further studies of function and mechanism for their actions.
Fresh normal (5 samples) and BUC (30 samples) tissues were collected from September 2008 to September 2009 in our hospital and analyzed by miRNA microarray to find differentially expressed miRNAs; RT-PCR (reverse transcription-polymerase chain reaction) of recollected samples and 4 types of cell strain to verify the selected miRNAs.
In grade I, grade II, grade III, grade I + II + III, infiltrating and non-infiltrating groups, hsa-miR-29b-1 was up-regulated while hsa-miR-923 and hsa-miR-300 were down-regulated. When the infiltrating BUC group was compared with the non-infiltrating BUC group, there were 7 differentially expressed miRNAs. In collected samples, the result of RT-PCR was consistent with that of miRNA array. In 4 types of cancer cell strains, only the result of T24 was completely consistent with that of miRNA array.
There are differentially expressed miRNAs between normal bladder mucosa and BUC, infiltration BUC and non-infiltration BUC. The result of T24 cell strain is completely consistent with that of miRNA array. |
| doi_str_mv | 10.3760/cma.j.issn.0376-2491.2010.48.002 |
| format | Article |
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Fresh normal (5 samples) and BUC (30 samples) tissues were collected from September 2008 to September 2009 in our hospital and analyzed by miRNA microarray to find differentially expressed miRNAs; RT-PCR (reverse transcription-polymerase chain reaction) of recollected samples and 4 types of cell strain to verify the selected miRNAs.
In grade I, grade II, grade III, grade I + II + III, infiltrating and non-infiltrating groups, hsa-miR-29b-1 was up-regulated while hsa-miR-923 and hsa-miR-300 were down-regulated. When the infiltrating BUC group was compared with the non-infiltrating BUC group, there were 7 differentially expressed miRNAs. In collected samples, the result of RT-PCR was consistent with that of miRNA array. In 4 types of cancer cell strains, only the result of T24 was completely consistent with that of miRNA array.
There are differentially expressed miRNAs between normal bladder mucosa and BUC, infiltration BUC and non-infiltration BUC. The result of T24 cell strain is completely consistent with that of miRNA array.</description><identifier>ISSN: 0376-2491</identifier><identifier>DOI: 10.3760/cma.j.issn.0376-2491.2010.48.002</identifier><identifier>PMID: 21223810</identifier><language>chi</language><publisher>China</publisher><subject>Adult ; Carcinoma, Transitional Cell - genetics ; Carcinoma, Transitional Cell - pathology ; Gene Expression Regulation, Neoplastic ; Humans ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Mucous Membrane - metabolism ; Mucous Membrane - pathology ; Neoplasm Staging ; Reverse Transcriptase Polymerase Chain Reaction ; Urinary Bladder Neoplasms - genetics ; Urinary Bladder Neoplasms - pathology ; Urothelium - metabolism ; Urothelium - pathology</subject><ispartof>Zhong hua yi xue za zhi, 2010-12, Vol.90 (48), p.3391-3394</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21223810$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xie, Peng</creatorcontrib><creatorcontrib>Xu, Feng</creatorcontrib><creatorcontrib>Cheng, Wen</creatorcontrib><creatorcontrib>Gao, Jian-Ping</creatorcontrib><creatorcontrib>Zhang, Zheng-Yu</creatorcontrib><creatorcontrib>Ge, Jing-Ping</creatorcontrib><creatorcontrib>Wei, Zhi-Feng</creatorcontrib><creatorcontrib>Xu, Xiao-Feng</creatorcontrib><title>Detection, verification and significance of differentially expressed miRNAs in bladder urothelial carcinoma</title><title>Zhong hua yi xue za zhi</title><addtitle>Zhonghua Yi Xue Za Zhi</addtitle><description>To explore the differentially expressed miRNAs in bladder urothelial carcinoma (BUC) in order to provide rationales for further studies of function and mechanism for their actions.
Fresh normal (5 samples) and BUC (30 samples) tissues were collected from September 2008 to September 2009 in our hospital and analyzed by miRNA microarray to find differentially expressed miRNAs; RT-PCR (reverse transcription-polymerase chain reaction) of recollected samples and 4 types of cell strain to verify the selected miRNAs.
In grade I, grade II, grade III, grade I + II + III, infiltrating and non-infiltrating groups, hsa-miR-29b-1 was up-regulated while hsa-miR-923 and hsa-miR-300 were down-regulated. When the infiltrating BUC group was compared with the non-infiltrating BUC group, there were 7 differentially expressed miRNAs. In collected samples, the result of RT-PCR was consistent with that of miRNA array. In 4 types of cancer cell strains, only the result of T24 was completely consistent with that of miRNA array.
There are differentially expressed miRNAs between normal bladder mucosa and BUC, infiltration BUC and non-infiltration BUC. The result of T24 cell strain is completely consistent with that of miRNA array.</description><subject>Adult</subject><subject>Carcinoma, Transitional Cell - genetics</subject><subject>Carcinoma, Transitional Cell - pathology</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Mucous Membrane - metabolism</subject><subject>Mucous Membrane - pathology</subject><subject>Neoplasm Staging</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Urinary Bladder Neoplasms - genetics</subject><subject>Urinary Bladder Neoplasms - pathology</subject><subject>Urothelium - metabolism</subject><subject>Urothelium - pathology</subject><issn>0376-2491</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMtOwzAQRb0A0ar0F5B3sCDBjziPZVWeUgUSgnU0ccZgSJxgJ4j-PSkUVqM598xIM4SccRbLLGUXuoX4LbYhuJhNIBJJwWPBpjjJY8bEAZn_8xlZhmArlmSyEEzwIzITXAiZczYn75c4oB5s587pJ3prrIZdR8HVNNgX90OcRtoZWltj0KMbLDTNluJX7zEErGlrH-9XgVpHqwbqGj0dfTe8YjOJVIPX1nUtHJNDA03A5b4uyPP11dP6Nto83NytV5uo5yofIkhMWmGqsqrIhM4MyyXIIhEAHCRKpYpa1XI6xyiWm5xzxcDoiiepECJVUi7I6e_e3ncfI4ahbG3Q2DTgsBtDmavd0ORO5sneHKsW67L3tgW_Lf_-I78BD3Vr6A</recordid><startdate>20101228</startdate><enddate>20101228</enddate><creator>Xie, Peng</creator><creator>Xu, Feng</creator><creator>Cheng, Wen</creator><creator>Gao, Jian-Ping</creator><creator>Zhang, Zheng-Yu</creator><creator>Ge, Jing-Ping</creator><creator>Wei, Zhi-Feng</creator><creator>Xu, Xiao-Feng</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20101228</creationdate><title>Detection, verification and significance of differentially expressed miRNAs in bladder urothelial carcinoma</title><author>Xie, Peng ; Xu, Feng ; Cheng, Wen ; Gao, Jian-Ping ; Zhang, Zheng-Yu ; Ge, Jing-Ping ; Wei, Zhi-Feng ; Xu, Xiao-Feng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p158t-a4f6be657b972c7f083a3942aa1a3e3559d5d3047f508f81150afcb1462226533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2010</creationdate><topic>Adult</topic><topic>Carcinoma, Transitional Cell - genetics</topic><topic>Carcinoma, Transitional Cell - pathology</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Mucous Membrane - metabolism</topic><topic>Mucous Membrane - pathology</topic><topic>Neoplasm Staging</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Urinary Bladder Neoplasms - genetics</topic><topic>Urinary Bladder Neoplasms - pathology</topic><topic>Urothelium - metabolism</topic><topic>Urothelium - pathology</topic><toplevel>online_resources</toplevel><creatorcontrib>Xie, Peng</creatorcontrib><creatorcontrib>Xu, Feng</creatorcontrib><creatorcontrib>Cheng, Wen</creatorcontrib><creatorcontrib>Gao, Jian-Ping</creatorcontrib><creatorcontrib>Zhang, Zheng-Yu</creatorcontrib><creatorcontrib>Ge, Jing-Ping</creatorcontrib><creatorcontrib>Wei, Zhi-Feng</creatorcontrib><creatorcontrib>Xu, Xiao-Feng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Zhong hua yi xue za zhi</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xie, Peng</au><au>Xu, Feng</au><au>Cheng, Wen</au><au>Gao, Jian-Ping</au><au>Zhang, Zheng-Yu</au><au>Ge, Jing-Ping</au><au>Wei, Zhi-Feng</au><au>Xu, Xiao-Feng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection, verification and significance of differentially expressed miRNAs in bladder urothelial carcinoma</atitle><jtitle>Zhong hua yi xue za zhi</jtitle><addtitle>Zhonghua Yi Xue Za Zhi</addtitle><date>2010-12-28</date><risdate>2010</risdate><volume>90</volume><issue>48</issue><spage>3391</spage><epage>3394</epage><pages>3391-3394</pages><issn>0376-2491</issn><abstract>To explore the differentially expressed miRNAs in bladder urothelial carcinoma (BUC) in order to provide rationales for further studies of function and mechanism for their actions.
Fresh normal (5 samples) and BUC (30 samples) tissues were collected from September 2008 to September 2009 in our hospital and analyzed by miRNA microarray to find differentially expressed miRNAs; RT-PCR (reverse transcription-polymerase chain reaction) of recollected samples and 4 types of cell strain to verify the selected miRNAs.
In grade I, grade II, grade III, grade I + II + III, infiltrating and non-infiltrating groups, hsa-miR-29b-1 was up-regulated while hsa-miR-923 and hsa-miR-300 were down-regulated. When the infiltrating BUC group was compared with the non-infiltrating BUC group, there were 7 differentially expressed miRNAs. In collected samples, the result of RT-PCR was consistent with that of miRNA array. In 4 types of cancer cell strains, only the result of T24 was completely consistent with that of miRNA array.
There are differentially expressed miRNAs between normal bladder mucosa and BUC, infiltration BUC and non-infiltration BUC. The result of T24 cell strain is completely consistent with that of miRNA array.</abstract><cop>China</cop><pmid>21223810</pmid><doi>10.3760/cma.j.issn.0376-2491.2010.48.002</doi><tpages>4</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adult Carcinoma, Transitional Cell - genetics Carcinoma, Transitional Cell - pathology Gene Expression Regulation, Neoplastic Humans MicroRNAs - genetics MicroRNAs - metabolism Mucous Membrane - metabolism Mucous Membrane - pathology Neoplasm Staging Reverse Transcriptase Polymerase Chain Reaction Urinary Bladder Neoplasms - genetics Urinary Bladder Neoplasms - pathology Urothelium - metabolism Urothelium - pathology |
| title | Detection, verification and significance of differentially expressed miRNAs in bladder urothelial carcinoma |
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