A first step towards practical single cell proteomics: a microfluidic antibody capture chip with TIRF detection

We have developed a generic platform to undertake the analysis of protein copy number from single cells. The approach described here is 'all-optical' whereby single cells are manipulated into separate analysis chambers using an optical trap; single cells are lysed by a shock wave caused by...

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Veröffentlicht in:	Lab on a chip 2011-04, Vol.11 (7), p.1256-1261
Hauptverfasser:	Salehi-Reyhani, Ali, Kaplinsky, Joseph, Burgin, Edward, Novakova, Miroslava, deMello, Andrew J, Templer, Richard H, Parker, Peter, Neil, Mark A A, Ces, Oscar, French, Paul, Willison, Keith R, Klug, David
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - immunology Cell Line, Tumor Humans Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Microscopy, Fluorescence - methods Proteomics - instrumentation Proteomics - methods Single-Cell Analysis - instrumentation Single-Cell Analysis - methods Tumor Suppressor Protein p53 - analysis Tumor Suppressor Protein p53 - immunology
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