Quantitative assessment of sterol traffic in living cells by dual labeling with dehydroergosterol and BODIPY-cholesterol

Cholesterol with BODIPY at carbon-24 of the side chain (BCh2) has recently been introduced as new cholesterol probe with superior fluorescence properties. We compare BCh2 with the intrinsically fluorescent dehydroergosterol (DHE), a well-established marker for cholesterol, by introducing simultaneou...

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Veröffentlicht in:	Chemistry and physics of lipids 2011-03, Vol.164 (3), p.221-235
Hauptverfasser:	Wüstner, Daniel, Solanko, Lukasz, Sokol, Elena, Garvik, Olav, Li, Zaiguo, Bittman, Robert, Korte, Thomas, Herrmann, Andreas
Format:	Artikel
Sprache:	eng
Schlagworte:	Boron Compounds - chemistry Boron Compounds - metabolism Cells, Cultured Cholesterol Cholesterol - chemistry Cholesterol - metabolism clathrin droplets energy Ergosterol - analogs & derivatives Ergosterol - chemistry Ergosterol - metabolism fluorescence Fluorescent Dyes - chemistry Fluorescent Dyes - metabolism Fluorescent probe HeLa Cells Humans image analysis lipid content Liquid-ordered phase Molecular Structure Partitioning Photobleaching photolysis plasma membrane Staining and Labeling Stereoisomerism Stretched exponential function Time-resolved fluorescence microscopy
Online-Zugang:	Volltext
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