Insertion of CTCF-binding sites into a first-generation adenovirus vector reduces the innate inflammatory response and prolongs transgene expression

Abstract We have made improvements to E1-deleted adenovirus (Ad) transducing vectors that both substantially reduce the innate inflammatory response provoked by the virus in BALB/c mouse ears and increase the duration of expression of the GFP transgene in BALB/c mouse liver. These improvements resul...

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Veröffentlicht in:	Virology (New York, N.Y.) N.Y.), 2011-03, Vol.412 (1), p.136-145
Hauptverfasser:	Schaack, Jerome, Qiao, Liping, Walkiewicz, Marcin P, Stonehouse, Martin, Engel, Daniel A, Vazquez-Torres, Andres, Nordeen, Steven K, Shao, Jianhua, Moorhead, John W
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenoviridae - genetics Adenoviridae - immunology Adenoviridae - pathogenicity Adenovirus vectors Animals Binding Sites Chromatin insulator CMV promoter CTCF Ear - pathology Ear - virology Enhancer Female Genes, Reporter Genetic Vectors - administration & dosage Genetic Vectors - immunology GFP Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Immunity, Innate Infectious Disease Inflammation Liver - virology Liver transduction Mice Mice, Inbred BALB C Mouse ear swelling Mutagenesis, Insertional pIX Time Factors Transduction, Genetic Transgenes
Online-Zugang:	Volltext
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