Chimeric porcine reproductive and respiratory syndrome viruses reveal full function of genotype 1 envelope proteins in the backbone of genotype 2
Abstract Porcine reproductive and respiratory syndrome virus (PRRSV) is classified into two genotypes, type 1 and type 2, which share only about 60% genetic identity. Here, we report viable chimeric viruses in which the envelope protein genes from ORF2a to ORF5 of vSHE (type 1) were swapped into the...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 2011-03, Vol.412 (1), p.1-8 |
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| creator | Tian, Debin Zheng, Haihong Zhang, Rong Zhuang, Jinshan Yuan, Shishan |
| description | Abstract Porcine reproductive and respiratory syndrome virus (PRRSV) is classified into two genotypes, type 1 and type 2, which share only about 60% genetic identity. Here, we report viable chimeric viruses in which the envelope protein genes from ORF2a to ORF5 of vSHE (type 1) were swapped into the genetic backbone of vAPRRS (type 2). We found that the envelope proteins of genotype 1 were fully functional in genotype 2 PRRSV, and the rescued chimeric progeny viruses showed robust genetic stability and similar replication properties to the parental strains in vitro . To our knowledge, this is the first study to report the substitution of complete ORFs between different genotypes of porcine arterivirus. These findings pave the way to further elucidate the structure–function relationship of PRRSV envelope proteins, and may enable the development of novel marker vaccines that can be used to differentiate vaccinated from infected animals. |
| doi_str_mv | 10.1016/j.virol.2010.12.048 |
| format | Article |
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Here, we report viable chimeric viruses in which the envelope protein genes from ORF2a to ORF5 of vSHE (type 1) were swapped into the genetic backbone of vAPRRS (type 2). We found that the envelope proteins of genotype 1 were fully functional in genotype 2 PRRSV, and the rescued chimeric progeny viruses showed robust genetic stability and similar replication properties to the parental strains in vitro . To our knowledge, this is the first study to report the substitution of complete ORFs between different genotypes of porcine arterivirus. These findings pave the way to further elucidate the structure–function relationship of PRRSV envelope proteins, and may enable the development of novel marker vaccines that can be used to differentiate vaccinated from infected animals.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/j.virol.2010.12.048</identifier><identifier>PMID: 21255814</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Line ; Chimeric ; Envelope proteins ; Genes, Viral ; Genomic Instability ; Genotype ; Infectious Disease ; Porcine reproductive and respiratory syndrome virus ; Porcine respiratory and reproductive syndrome virus - genetics ; Porcine respiratory and reproductive syndrome virus - physiology ; Recombination, Genetic ; RNA, Viral - genetics ; Swine ; Two genotypes ; Viral Envelope Proteins - genetics ; Viral Envelope Proteins - metabolism ; Virus Replication</subject><ispartof>Virology (New York, N.Y.), 2011-03, Vol.412 (1), p.1-8</ispartof><rights>Elsevier Inc.</rights><rights>2010 Elsevier Inc.</rights><rights>Copyright © 2010 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-550f91f653790a3c452af7eee21f9f5631e6476d9ba95a918e5714e8aec4a3313</citedby><cites>FETCH-LOGICAL-c413t-550f91f653790a3c452af7eee21f9f5631e6476d9ba95a918e5714e8aec4a3313</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.virol.2010.12.048$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21255814$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tian, Debin</creatorcontrib><creatorcontrib>Zheng, Haihong</creatorcontrib><creatorcontrib>Zhang, Rong</creatorcontrib><creatorcontrib>Zhuang, Jinshan</creatorcontrib><creatorcontrib>Yuan, Shishan</creatorcontrib><title>Chimeric porcine reproductive and respiratory syndrome viruses reveal full function of genotype 1 envelope proteins in the backbone of genotype 2</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>Abstract Porcine reproductive and respiratory syndrome virus (PRRSV) is classified into two genotypes, type 1 and type 2, which share only about 60% genetic identity. 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These findings pave the way to further elucidate the structure–function relationship of PRRSV envelope proteins, and may enable the development of novel marker vaccines that can be used to differentiate vaccinated from infected animals.</description><subject>Animals</subject><subject>Cell Line</subject><subject>Chimeric</subject><subject>Envelope proteins</subject><subject>Genes, Viral</subject><subject>Genomic Instability</subject><subject>Genotype</subject><subject>Infectious Disease</subject><subject>Porcine reproductive and respiratory syndrome virus</subject><subject>Porcine respiratory and reproductive syndrome virus - genetics</subject><subject>Porcine respiratory and reproductive syndrome virus - physiology</subject><subject>Recombination, Genetic</subject><subject>RNA, Viral - genetics</subject><subject>Swine</subject><subject>Two genotypes</subject><subject>Viral Envelope Proteins - genetics</subject><subject>Viral Envelope Proteins - metabolism</subject><subject>Virus Replication</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUk2PFCEQJUbjjqO_wMRw89QjBU1_HDQxE7-STTyoZ8LQhctsN7TQ3Un_jP3H0s5qjBcvQBXvvYJ6RchzYAdgUL06HxYXQ3_gbMvwAyubB2QHrK0KJkp4SHaMlbyoGs6vyJOUzizHdc0ekysOXMoGyh25O964AaMzdAzROI804hhDN5vJLUi173IijS7qKcSVptV3MQxIc-k5YcqXC-qe2rnfFp9ZwdNg6Xf0YVpHpEDRL9iHfMy6EzqfqPN0ukF60ub2FHLJv_H8KXlkdZ_w2f2-J9_ev_t6_Fhcf_7w6fj2ujAliKmQktkWbCVF3TItTCm5tjUicrCtlZUArMq66tqTbqVuoUFZQ4mNRlNqIUDsycuLbn7WjxnTpAaXDPa99hjmpJqM55xlpT0RF6SJIaWIVo3RDTquCpjarFBn9csKtVmhgKtsRWa9uNefTwN2fzi_e58Bry8AzL9cHEaVjENvsHMRzaS64P5T4M0_fNM774zub3HFdA5z9LmBClTKBPVlm4ZtGCDPQQMVEz8B8UazNQ</recordid><startdate>20110330</startdate><enddate>20110330</enddate><creator>Tian, Debin</creator><creator>Zheng, Haihong</creator><creator>Zhang, Rong</creator><creator>Zhuang, Jinshan</creator><creator>Yuan, Shishan</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110330</creationdate><title>Chimeric porcine reproductive and respiratory syndrome viruses reveal full function of genotype 1 envelope proteins in the backbone of genotype 2</title><author>Tian, Debin ; 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| source | MEDLINE; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Cell Line Chimeric Envelope proteins Genes, Viral Genomic Instability Genotype Infectious Disease Porcine reproductive and respiratory syndrome virus Porcine respiratory and reproductive syndrome virus - genetics Porcine respiratory and reproductive syndrome virus - physiology Recombination, Genetic RNA, Viral - genetics Swine Two genotypes Viral Envelope Proteins - genetics Viral Envelope Proteins - metabolism Virus Replication |
| title | Chimeric porcine reproductive and respiratory syndrome viruses reveal full function of genotype 1 envelope proteins in the backbone of genotype 2 |
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