Phylogenetic background and virulence genes of Escherichia coli isolates from colisepticemic and healthy broiler chickens in Iran
The purposes of this study were to determine the phylogenetic background and the virulence gene profiles of Escherichia coli isolates from colisepticemic and feces of healthy (AFEC) broiler chickens. In this study, 253 E. coli isolates including 141 avian pathogenic E. coli (APEC) and 112 AFEC isola...
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| Veröffentlicht in: | Tropical animal health and production 2011, Vol.43 (1), p.153-157 |
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| description | The purposes of this study were to determine the phylogenetic background and the virulence gene profiles of Escherichia coli isolates from colisepticemic and feces of healthy (AFEC) broiler chickens. In this study, 253 E. coli isolates including 141 avian pathogenic E. coli (APEC) and 112 AFEC isolates were examined by PCR. In general, 253 E. coli isolates distributed among group A (51.8%), B1 (15.8%), B2 (8.7%), and D (23.7%). Ten (8.9%) AFEC isolates segregated in to B1 phylo-group and 102 (91.1%) isolates fell into six different phylogenetic subgroups. Distribution of colisepticemic and fecal isolates differed significantly in their assignments to A and B1 phylo-groups. The three most prevalent virulence genes were crl, fimH, and aer in isolates between both groups. The four genetic markers aer, papC, afa, and sfa were detected significantly more often among colisepticemic isolates than in fecal isolates from healthy broilers. The presence of stx ₂ gene in fecal isolates were significantly differs among the colisepticemic isolates. F17 fimbrial family encoding gene and eae gene were detected in APEC and AFEC isolates, respectively. The colisepticemic and fecal isolates possessed the virulence genes were detected in all of the four phylogenetic groups. Several combination patterns of the virulence genes were detected in APEC and AFEC isolates. In colisepticemic isolates the combination of aer, crl, and fimH genes was the most prevalent pattern. None of the examined isolates harbored the cdt, cnf1, ipaH, and stx ₁ virulence gene sequences. |
| doi_str_mv | 10.1007/s11250-010-9667-2 |
| format | Article |
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In this study, 253 E. coli isolates including 141 avian pathogenic E. coli (APEC) and 112 AFEC isolates were examined by PCR. In general, 253 E. coli isolates distributed among group A (51.8%), B1 (15.8%), B2 (8.7%), and D (23.7%). Ten (8.9%) AFEC isolates segregated in to B1 phylo-group and 102 (91.1%) isolates fell into six different phylogenetic subgroups. Distribution of colisepticemic and fecal isolates differed significantly in their assignments to A and B1 phylo-groups. The three most prevalent virulence genes were crl, fimH, and aer in isolates between both groups. The four genetic markers aer, papC, afa, and sfa were detected significantly more often among colisepticemic isolates than in fecal isolates from healthy broilers. The presence of stx ₂ gene in fecal isolates were significantly differs among the colisepticemic isolates. F17 fimbrial family encoding gene and eae gene were detected in APEC and AFEC isolates, respectively. The colisepticemic and fecal isolates possessed the virulence genes were detected in all of the four phylogenetic groups. Several combination patterns of the virulence genes were detected in APEC and AFEC isolates. In colisepticemic isolates the combination of aer, crl, and fimH genes was the most prevalent pattern. None of the examined isolates harbored the cdt, cnf1, ipaH, and stx ₁ virulence gene sequences.</description><identifier>ISSN: 0049-4747</identifier><identifier>EISSN: 1573-7438</identifier><identifier>DOI: 10.1007/s11250-010-9667-2</identifier><identifier>PMID: 20680444</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Animals ; Biomedical and Life Sciences ; Chickens ; E coli ; Escherichia coli ; Escherichia coli - genetics ; Escherichia coli - pathogenicity ; Escherichia coli Infections - genetics ; Escherichia coli Infections - veterinary ; Feces - microbiology ; Genetic markers ; Genetic Markers - genetics ; Life Sciences ; Original Research ; Phylogenetic background ; Phylogeny ; Polymerase Chain Reaction ; poultry ; Poultry Diseases - microbiology ; Sepsis - microbiology ; Veterinary Medicine/Veterinary Science ; Virulence ; Virulence genes ; Zoology</subject><ispartof>Tropical animal health and production, 2011, Vol.43 (1), p.153-157</ispartof><rights>Springer Science+Business Media B.V. 2010</rights><rights>Springer Science+Business Media B.V. 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-c4c2aa15c283860101369f13d4fd17d5eeec6f2e1a666cb0065848f35fa0aa493</citedby><cites>FETCH-LOGICAL-c426t-c4c2aa15c283860101369f13d4fd17d5eeec6f2e1a666cb0065848f35fa0aa493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11250-010-9667-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11250-010-9667-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,4025,27928,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20680444$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ghanbarpour, Reza</creatorcontrib><creatorcontrib>Sami, Masoud</creatorcontrib><creatorcontrib>Salehi, Mahmood</creatorcontrib><creatorcontrib>Ouromiei, Mahla</creatorcontrib><title>Phylogenetic background and virulence genes of Escherichia coli isolates from colisepticemic and healthy broiler chickens in Iran</title><title>Tropical animal health and production</title><addtitle>Trop Anim Health Prod</addtitle><addtitle>Trop Anim Health Prod</addtitle><description>The purposes of this study were to determine the phylogenetic background and the virulence gene profiles of Escherichia coli isolates from colisepticemic and feces of healthy (AFEC) broiler chickens. In this study, 253 E. coli isolates including 141 avian pathogenic E. coli (APEC) and 112 AFEC isolates were examined by PCR. In general, 253 E. coli isolates distributed among group A (51.8%), B1 (15.8%), B2 (8.7%), and D (23.7%). Ten (8.9%) AFEC isolates segregated in to B1 phylo-group and 102 (91.1%) isolates fell into six different phylogenetic subgroups. Distribution of colisepticemic and fecal isolates differed significantly in their assignments to A and B1 phylo-groups. The three most prevalent virulence genes were crl, fimH, and aer in isolates between both groups. The four genetic markers aer, papC, afa, and sfa were detected significantly more often among colisepticemic isolates than in fecal isolates from healthy broilers. The presence of stx ₂ gene in fecal isolates were significantly differs among the colisepticemic isolates. F17 fimbrial family encoding gene and eae gene were detected in APEC and AFEC isolates, respectively. The colisepticemic and fecal isolates possessed the virulence genes were detected in all of the four phylogenetic groups. Several combination patterns of the virulence genes were detected in APEC and AFEC isolates. In colisepticemic isolates the combination of aer, crl, and fimH genes was the most prevalent pattern. None of the examined isolates harbored the cdt, cnf1, ipaH, and stx ₁ virulence gene sequences.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Chickens</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - pathogenicity</subject><subject>Escherichia coli Infections - genetics</subject><subject>Escherichia coli Infections - veterinary</subject><subject>Feces - microbiology</subject><subject>Genetic markers</subject><subject>Genetic Markers - genetics</subject><subject>Life Sciences</subject><subject>Original Research</subject><subject>Phylogenetic background</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>poultry</subject><subject>Poultry Diseases - microbiology</subject><subject>Sepsis - microbiology</subject><subject>Veterinary Medicine/Veterinary Science</subject><subject>Virulence</subject><subject>Virulence genes</subject><subject>Zoology</subject><issn>0049-4747</issn><issn>1573-7438</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkUFvFSEUhYnR2Gf1B7hR4qarUWAYYJamqbVJkzbRrgmPubyhZYYnzDR5S_-5TKdq4qIugIT7nQP3HoTeUvKREiI_ZUpZQypCSdUKISv2DG1oI-tK8lo9RxtCeFtxyeURepXzLSkgUeIlOmJEKMI536Cf1_0hxB2MMHmLt8be7VKcxw6bsu59mgOMFvACZBwdPsu2h-Rt7w22MXjscwxmKkWX4vBwlWFfvGAofotJDyZM_QFvU_QBEi5Sewdjxn7EF8mMr9ELZ0KGN4_nMbr5cvb99Gt1eXV-cfr5srKcianslhlDG8tUrUTphNaidbTuuOuo7BoAsMIxoEYIYbeEiEZx5erGGWIMb-tjdLL67lP8MUOe9OCzhRDMCHHOWjVCqrYM5f8kbVpBiVo8P_xD3sY5jaWNBWItY7IuEF0hm2LOCZzeJz-YdNCU6CVHveaoS1N6yVGzonn3aDxvB-j-KH4HVwC2ArmUxh2kvy8_5fp-FTkTtdkln_XNN1YmSWhLJS-f_QUQPLJ6</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>Ghanbarpour, Reza</creator><creator>Sami, Masoud</creator><creator>Salehi, Mahmood</creator><creator>Ouromiei, Mahla</creator><general>Dordrecht : Springer Netherlands</general><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U7</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>RC3</scope></search><sort><creationdate>2011</creationdate><title>Phylogenetic background and virulence genes of Escherichia coli isolates from colisepticemic and healthy broiler chickens in Iran</title><author>Ghanbarpour, Reza ; Sami, Masoud ; Salehi, Mahmood ; Ouromiei, Mahla</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-c4c2aa15c283860101369f13d4fd17d5eeec6f2e1a666cb0065848f35fa0aa493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Chickens</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - pathogenicity</topic><topic>Escherichia coli Infections - genetics</topic><topic>Escherichia coli Infections - veterinary</topic><topic>Feces - microbiology</topic><topic>Genetic markers</topic><topic>Genetic Markers - genetics</topic><topic>Life Sciences</topic><topic>Original Research</topic><topic>Phylogenetic background</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction</topic><topic>poultry</topic><topic>Poultry Diseases - microbiology</topic><topic>Sepsis - microbiology</topic><topic>Veterinary Medicine/Veterinary Science</topic><topic>Virulence</topic><topic>Virulence genes</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ghanbarpour, Reza</creatorcontrib><creatorcontrib>Sami, Masoud</creatorcontrib><creatorcontrib>Salehi, Mahmood</creatorcontrib><creatorcontrib>Ouromiei, Mahla</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>Genetics Abstracts</collection><jtitle>Tropical animal health and production</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ghanbarpour, Reza</au><au>Sami, Masoud</au><au>Salehi, Mahmood</au><au>Ouromiei, Mahla</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phylogenetic background and virulence genes of Escherichia coli isolates from colisepticemic and healthy broiler chickens in Iran</atitle><jtitle>Tropical animal health and production</jtitle><stitle>Trop Anim Health Prod</stitle><addtitle>Trop Anim Health Prod</addtitle><date>2011</date><risdate>2011</risdate><volume>43</volume><issue>1</issue><spage>153</spage><epage>157</epage><pages>153-157</pages><issn>0049-4747</issn><eissn>1573-7438</eissn><abstract>The purposes of this study were to determine the phylogenetic background and the virulence gene profiles of Escherichia coli isolates from colisepticemic and feces of healthy (AFEC) broiler chickens. In this study, 253 E. coli isolates including 141 avian pathogenic E. coli (APEC) and 112 AFEC isolates were examined by PCR. In general, 253 E. coli isolates distributed among group A (51.8%), B1 (15.8%), B2 (8.7%), and D (23.7%). Ten (8.9%) AFEC isolates segregated in to B1 phylo-group and 102 (91.1%) isolates fell into six different phylogenetic subgroups. Distribution of colisepticemic and fecal isolates differed significantly in their assignments to A and B1 phylo-groups. The three most prevalent virulence genes were crl, fimH, and aer in isolates between both groups. The four genetic markers aer, papC, afa, and sfa were detected significantly more often among colisepticemic isolates than in fecal isolates from healthy broilers. The presence of stx ₂ gene in fecal isolates were significantly differs among the colisepticemic isolates. F17 fimbrial family encoding gene and eae gene were detected in APEC and AFEC isolates, respectively. The colisepticemic and fecal isolates possessed the virulence genes were detected in all of the four phylogenetic groups. Several combination patterns of the virulence genes were detected in APEC and AFEC isolates. In colisepticemic isolates the combination of aer, crl, and fimH genes was the most prevalent pattern. None of the examined isolates harbored the cdt, cnf1, ipaH, and stx ₁ virulence gene sequences.</abstract><cop>Dordrecht</cop><pub>Dordrecht : Springer Netherlands</pub><pmid>20680444</pmid><doi>10.1007/s11250-010-9667-2</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Biomedical and Life Sciences Chickens E coli Escherichia coli Escherichia coli - genetics Escherichia coli - pathogenicity Escherichia coli Infections - genetics Escherichia coli Infections - veterinary Feces - microbiology Genetic markers Genetic Markers - genetics Life Sciences Original Research Phylogenetic background Phylogeny Polymerase Chain Reaction poultry Poultry Diseases - microbiology Sepsis - microbiology Veterinary Medicine/Veterinary Science Virulence Virulence genes Zoology |
| title | Phylogenetic background and virulence genes of Escherichia coli isolates from colisepticemic and healthy broiler chickens in Iran |
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