Estrogen receptors in medaka ( Oryzias latipes) and estrogenic environmental contaminants: An in vitro– in vivo correlation
▶ In fish, as in other vertebrates, estrogen, primarily estradiol-17β(E2), plays an important role in the regulation of various processes of reproduction. These actions of E2 are known to be mediated by estrogen receptors (ERs). Medaka ( Oryzias latipes) has three estrogen receptor (ER) subtypes, ER...
Gespeichert in:
| Veröffentlicht in: | The Journal of steroid biochemistry and molecular biology 2011-02, Vol.123 (3), p.115-121 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 121 |
|---|---|
| container_issue | 3 |
| container_start_page | 115 |
| container_title | The Journal of steroid biochemistry and molecular biology |
| container_volume | 123 |
| creator | Chakraborty, Tapas Katsu, Yoshinao Zhou, Lin Yan Miyagawa, Shinichi Nagahama, Yoshitaka Iguchi, Taisen |
| description | ▶ In fish, as in other vertebrates, estrogen, primarily estradiol-17β(E2), plays an important role in the regulation of various processes of reproduction. These actions of E2 are known to be mediated by estrogen receptors (ERs). Medaka (
Oryzias latipes) has three estrogen receptor (ER) subtypes,
ERα,
ERβ1 and
ERβ2. In this investigation, we focused on characterization of each subtype of medaka ER via documenting trans-activation capacity of different ERs to various chemicals and effect of E
2 treatment during adulthood. 17β-Estradiol (E
2) and
op’-DDT showed high activation irrespective of ERs. Endosulfan also exhibited activation; with less/no transactivity measured using other pesticides, i.e., heptachlor, carbendazim, deltamethrin, acephate, dimethoate and amitraz. It was generally observed that
ERβ2 has higher activation potential than other two. The present data suggest that
ERβ2 is essential for female gonad maintenance. This was further supported by
ERβ2 co-relative induction of vitellogenin (
VTG) mRNA in the liver and reduced
VTG receptor mRNA expression in the gonad of both sexes. The present work will provide a basic tool allowing future studies to examine the receptor–ligand interactions and endocrine disrupting mechanisms and also expands our knowledge of estrogen action on reproductive development in medaka.
In many vertebrates, estrogens are necessary to promote the growth and differentiation of the female reproductive system during development, and have important reproductive roles in both males and females. Medaka (
Oryzias latipes) has three estrogen receptor (ER) subtypes,
ERα,
ERβ1 and
ERβ2. To evaluate the three medaka ER (mER)-ligand interactions, we applied the ERE-luciferase reporter assay system to characterize each ER subtype. In this transient transfection assay system using mammalian cells, the mER proteins displayed estrogen-dependent activation. 17β-Estradiol (E
2) and
op’-DDT showed high activation irrespective of ERs. Endosulfan also exhibited activation; with less/no transactivity measured using other pesticides, i.e., heptachlor, carbendazim, deltamethrin, acephate, dimethoate and amitraz. It was generally observed that
ERβ2 had higher activation potential than
ERα and
ERβ1. To understand the molecular mechanism of estrogen action via ER, we also conducted E
2 treatment where we observed a trigger in
ERβ2 expression upon E
2 exposure. The present data suggest that
ERβ2 is essential for female gonad maintenance. The data w |
| doi_str_mv | 10.1016/j.jsbmb.2010.11.015 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_856789208</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0960076010003742</els_id><sourcerecordid>856789208</sourcerecordid><originalsourceid>FETCH-LOGICAL-c420t-a65ab16b8178bb39f5e7270a95a2f710ae56ac1005d8bc3bedf36ccc1d3896c13</originalsourceid><addsrcrecordid>eNqFkdFqFDEUhoNY7Fp9AkFyI-rFrCfJTpIpeFFKbYVCb_Q6ZDJnJOtMsiazCxWEvkPf0Ccx4271Tq9OCN_5Oed8hLxgsGTA5Lv1cp3bsV1ymH_YElj9iCyYVk3FOIfHZAGNhAqUhGPyNOc1AAjB1BNyzBlb1Y2UC_LjIk8pfsFAEzrcTDFl6gMdsbNfLX1Db9Ltd28zHezkN5jfUhs6ioce7yiGnU8xjBgmO1AXSxl9sGHKp_QszFE7X-Cfd_f79y4WKCWc82J4Ro56O2R8fqgn5POHi0_nV9X1zeXH87Pryq04TJWVtW2ZbDVTum1F09eouALb1Jb3ioHFWlrHAOpOt0602PVCOudYJ3QjHRMn5PU-d5Pit20Z34w-OxwGGzBus9G1VLrhoP9PrrTgoIQqpNiTLsWcE_Zmk_xo061hYGZBZm1-CzKzIMOYKYJK18tD_rYtR_7T82CkAK8OgM3ODn2ywfn8lxN6xQWfV3q_57Dcbecxmew8BoedLyYn00X_z0F-AXt0siM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>848320737</pqid></control><display><type>article</type><title>Estrogen receptors in medaka ( Oryzias latipes) and estrogenic environmental contaminants: An in vitro– in vivo correlation</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Chakraborty, Tapas ; Katsu, Yoshinao ; Zhou, Lin Yan ; Miyagawa, Shinichi ; Nagahama, Yoshitaka ; Iguchi, Taisen</creator><creatorcontrib>Chakraborty, Tapas ; Katsu, Yoshinao ; Zhou, Lin Yan ; Miyagawa, Shinichi ; Nagahama, Yoshitaka ; Iguchi, Taisen</creatorcontrib><description>▶ In fish, as in other vertebrates, estrogen, primarily estradiol-17β(E2), plays an important role in the regulation of various processes of reproduction. These actions of E2 are known to be mediated by estrogen receptors (ERs). Medaka (
Oryzias latipes) has three estrogen receptor (ER) subtypes,
ERα,
ERβ1 and
ERβ2. In this investigation, we focused on characterization of each subtype of medaka ER via documenting trans-activation capacity of different ERs to various chemicals and effect of E
2 treatment during adulthood. 17β-Estradiol (E
2) and
op’-DDT showed high activation irrespective of ERs. Endosulfan also exhibited activation; with less/no transactivity measured using other pesticides, i.e., heptachlor, carbendazim, deltamethrin, acephate, dimethoate and amitraz. It was generally observed that
ERβ2 has higher activation potential than other two. The present data suggest that
ERβ2 is essential for female gonad maintenance. This was further supported by
ERβ2 co-relative induction of vitellogenin (
VTG) mRNA in the liver and reduced
VTG receptor mRNA expression in the gonad of both sexes. The present work will provide a basic tool allowing future studies to examine the receptor–ligand interactions and endocrine disrupting mechanisms and also expands our knowledge of estrogen action on reproductive development in medaka.
In many vertebrates, estrogens are necessary to promote the growth and differentiation of the female reproductive system during development, and have important reproductive roles in both males and females. Medaka (
Oryzias latipes) has three estrogen receptor (ER) subtypes,
ERα,
ERβ1 and
ERβ2. To evaluate the three medaka ER (mER)-ligand interactions, we applied the ERE-luciferase reporter assay system to characterize each ER subtype. In this transient transfection assay system using mammalian cells, the mER proteins displayed estrogen-dependent activation. 17β-Estradiol (E
2) and
op’-DDT showed high activation irrespective of ERs. Endosulfan also exhibited activation; with less/no transactivity measured using other pesticides, i.e., heptachlor, carbendazim, deltamethrin, acephate, dimethoate and amitraz. It was generally observed that
ERβ2 had higher activation potential than
ERα and
ERβ1. To understand the molecular mechanism of estrogen action via ER, we also conducted E
2 treatment where we observed a trigger in
ERβ2 expression upon E
2 exposure. The present data suggest that
ERβ2 is essential for female gonad maintenance. The data were supported by induction of vitellogenin (
VTG) mRNA in the liver and reduced
VTG receptor mRNA expression in the gonad of both sexes. The present work will provide a basic tool allowing future studies to examine the receptor–ligand interactions and endocrine disrupting mechanisms, and also expands our knowledge of estrogen action on reproductive development in medaka.</description><identifier>ISSN: 0960-0760</identifier><identifier>EISSN: 1879-1220</identifier><identifier>DOI: 10.1016/j.jsbmb.2010.11.015</identifier><identifier>PMID: 21145966</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Animals ; Biological and medical sciences ; Brain ; Environmental Exposure - adverse effects ; Environmental Pollutants - pharmacology ; Estrogen receptors ; Estrogenic contaminants ; Estrogens - pharmacology ; Female ; Fish Proteins - genetics ; Fish Proteins - metabolism ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Gonads ; In Situ Hybridization, Fluorescence ; Liver ; Male ; Medaka ; Oryzias - metabolism ; Oryzias latipes ; Pesticides - pharmacology ; Receptors, Estrogen - genetics ; Receptors, Estrogen - metabolism ; RNA, Messenger - metabolism ; Vertebrates: endocrinology ; Vertebrates: reproduction ; Vitellogenins - genetics ; Vitellogenins - metabolism</subject><ispartof>The Journal of steroid biochemistry and molecular biology, 2011-02, Vol.123 (3), p.115-121</ispartof><rights>2010 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-a65ab16b8178bb39f5e7270a95a2f710ae56ac1005d8bc3bedf36ccc1d3896c13</citedby><cites>FETCH-LOGICAL-c420t-a65ab16b8178bb39f5e7270a95a2f710ae56ac1005d8bc3bedf36ccc1d3896c13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0960076010003742$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23842321$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21145966$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chakraborty, Tapas</creatorcontrib><creatorcontrib>Katsu, Yoshinao</creatorcontrib><creatorcontrib>Zhou, Lin Yan</creatorcontrib><creatorcontrib>Miyagawa, Shinichi</creatorcontrib><creatorcontrib>Nagahama, Yoshitaka</creatorcontrib><creatorcontrib>Iguchi, Taisen</creatorcontrib><title>Estrogen receptors in medaka ( Oryzias latipes) and estrogenic environmental contaminants: An in vitro– in vivo correlation</title><title>The Journal of steroid biochemistry and molecular biology</title><addtitle>J Steroid Biochem Mol Biol</addtitle><description>▶ In fish, as in other vertebrates, estrogen, primarily estradiol-17β(E2), plays an important role in the regulation of various processes of reproduction. These actions of E2 are known to be mediated by estrogen receptors (ERs). Medaka (
Oryzias latipes) has three estrogen receptor (ER) subtypes,
ERα,
ERβ1 and
ERβ2. In this investigation, we focused on characterization of each subtype of medaka ER via documenting trans-activation capacity of different ERs to various chemicals and effect of E
2 treatment during adulthood. 17β-Estradiol (E
2) and
op’-DDT showed high activation irrespective of ERs. Endosulfan also exhibited activation; with less/no transactivity measured using other pesticides, i.e., heptachlor, carbendazim, deltamethrin, acephate, dimethoate and amitraz. It was generally observed that
ERβ2 has higher activation potential than other two. The present data suggest that
ERβ2 is essential for female gonad maintenance. This was further supported by
ERβ2 co-relative induction of vitellogenin (
VTG) mRNA in the liver and reduced
VTG receptor mRNA expression in the gonad of both sexes. The present work will provide a basic tool allowing future studies to examine the receptor–ligand interactions and endocrine disrupting mechanisms and also expands our knowledge of estrogen action on reproductive development in medaka.
In many vertebrates, estrogens are necessary to promote the growth and differentiation of the female reproductive system during development, and have important reproductive roles in both males and females. Medaka (
Oryzias latipes) has three estrogen receptor (ER) subtypes,
ERα,
ERβ1 and
ERβ2. To evaluate the three medaka ER (mER)-ligand interactions, we applied the ERE-luciferase reporter assay system to characterize each ER subtype. In this transient transfection assay system using mammalian cells, the mER proteins displayed estrogen-dependent activation. 17β-Estradiol (E
2) and
op’-DDT showed high activation irrespective of ERs. Endosulfan also exhibited activation; with less/no transactivity measured using other pesticides, i.e., heptachlor, carbendazim, deltamethrin, acephate, dimethoate and amitraz. It was generally observed that
ERβ2 had higher activation potential than
ERα and
ERβ1. To understand the molecular mechanism of estrogen action via ER, we also conducted E
2 treatment where we observed a trigger in
ERβ2 expression upon E
2 exposure. The present data suggest that
ERβ2 is essential for female gonad maintenance. The data were supported by induction of vitellogenin (
VTG) mRNA in the liver and reduced
VTG receptor mRNA expression in the gonad of both sexes. The present work will provide a basic tool allowing future studies to examine the receptor–ligand interactions and endocrine disrupting mechanisms, and also expands our knowledge of estrogen action on reproductive development in medaka.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brain</subject><subject>Environmental Exposure - adverse effects</subject><subject>Environmental Pollutants - pharmacology</subject><subject>Estrogen receptors</subject><subject>Estrogenic contaminants</subject><subject>Estrogens - pharmacology</subject><subject>Female</subject><subject>Fish Proteins - genetics</subject><subject>Fish Proteins - metabolism</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gonads</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Liver</subject><subject>Male</subject><subject>Medaka</subject><subject>Oryzias - metabolism</subject><subject>Oryzias latipes</subject><subject>Pesticides - pharmacology</subject><subject>Receptors, Estrogen - genetics</subject><subject>Receptors, Estrogen - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Vertebrates: endocrinology</subject><subject>Vertebrates: reproduction</subject><subject>Vitellogenins - genetics</subject><subject>Vitellogenins - metabolism</subject><issn>0960-0760</issn><issn>1879-1220</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkdFqFDEUhoNY7Fp9AkFyI-rFrCfJTpIpeFFKbYVCb_Q6ZDJnJOtMsiazCxWEvkPf0Ccx4271Tq9OCN_5Oed8hLxgsGTA5Lv1cp3bsV1ymH_YElj9iCyYVk3FOIfHZAGNhAqUhGPyNOc1AAjB1BNyzBlb1Y2UC_LjIk8pfsFAEzrcTDFl6gMdsbNfLX1Db9Ltd28zHezkN5jfUhs6ioce7yiGnU8xjBgmO1AXSxl9sGHKp_QszFE7X-Cfd_f79y4WKCWc82J4Ro56O2R8fqgn5POHi0_nV9X1zeXH87Pryq04TJWVtW2ZbDVTum1F09eouALb1Jb3ioHFWlrHAOpOt0602PVCOudYJ3QjHRMn5PU-d5Pit20Z34w-OxwGGzBus9G1VLrhoP9PrrTgoIQqpNiTLsWcE_Zmk_xo061hYGZBZm1-CzKzIMOYKYJK18tD_rYtR_7T82CkAK8OgM3ODn2ywfn8lxN6xQWfV3q_57Dcbecxmew8BoedLyYn00X_z0F-AXt0siM</recordid><startdate>20110201</startdate><enddate>20110201</enddate><creator>Chakraborty, Tapas</creator><creator>Katsu, Yoshinao</creator><creator>Zhou, Lin Yan</creator><creator>Miyagawa, Shinichi</creator><creator>Nagahama, Yoshitaka</creator><creator>Iguchi, Taisen</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>20110201</creationdate><title>Estrogen receptors in medaka ( Oryzias latipes) and estrogenic environmental contaminants: An in vitro– in vivo correlation</title><author>Chakraborty, Tapas ; Katsu, Yoshinao ; Zhou, Lin Yan ; Miyagawa, Shinichi ; Nagahama, Yoshitaka ; Iguchi, Taisen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-a65ab16b8178bb39f5e7270a95a2f710ae56ac1005d8bc3bedf36ccc1d3896c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brain</topic><topic>Environmental Exposure - adverse effects</topic><topic>Environmental Pollutants - pharmacology</topic><topic>Estrogen receptors</topic><topic>Estrogenic contaminants</topic><topic>Estrogens - pharmacology</topic><topic>Female</topic><topic>Fish Proteins - genetics</topic><topic>Fish Proteins - metabolism</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gonads</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Liver</topic><topic>Male</topic><topic>Medaka</topic><topic>Oryzias - metabolism</topic><topic>Oryzias latipes</topic><topic>Pesticides - pharmacology</topic><topic>Receptors, Estrogen - genetics</topic><topic>Receptors, Estrogen - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Vertebrates: endocrinology</topic><topic>Vertebrates: reproduction</topic><topic>Vitellogenins - genetics</topic><topic>Vitellogenins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chakraborty, Tapas</creatorcontrib><creatorcontrib>Katsu, Yoshinao</creatorcontrib><creatorcontrib>Zhou, Lin Yan</creatorcontrib><creatorcontrib>Miyagawa, Shinichi</creatorcontrib><creatorcontrib>Nagahama, Yoshitaka</creatorcontrib><creatorcontrib>Iguchi, Taisen</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>The Journal of steroid biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chakraborty, Tapas</au><au>Katsu, Yoshinao</au><au>Zhou, Lin Yan</au><au>Miyagawa, Shinichi</au><au>Nagahama, Yoshitaka</au><au>Iguchi, Taisen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Estrogen receptors in medaka ( Oryzias latipes) and estrogenic environmental contaminants: An in vitro– in vivo correlation</atitle><jtitle>The Journal of steroid biochemistry and molecular biology</jtitle><addtitle>J Steroid Biochem Mol Biol</addtitle><date>2011-02-01</date><risdate>2011</risdate><volume>123</volume><issue>3</issue><spage>115</spage><epage>121</epage><pages>115-121</pages><issn>0960-0760</issn><eissn>1879-1220</eissn><abstract>▶ In fish, as in other vertebrates, estrogen, primarily estradiol-17β(E2), plays an important role in the regulation of various processes of reproduction. These actions of E2 are known to be mediated by estrogen receptors (ERs). Medaka (
Oryzias latipes) has three estrogen receptor (ER) subtypes,
ERα,
ERβ1 and
ERβ2. In this investigation, we focused on characterization of each subtype of medaka ER via documenting trans-activation capacity of different ERs to various chemicals and effect of E
2 treatment during adulthood. 17β-Estradiol (E
2) and
op’-DDT showed high activation irrespective of ERs. Endosulfan also exhibited activation; with less/no transactivity measured using other pesticides, i.e., heptachlor, carbendazim, deltamethrin, acephate, dimethoate and amitraz. It was generally observed that
ERβ2 has higher activation potential than other two. The present data suggest that
ERβ2 is essential for female gonad maintenance. This was further supported by
ERβ2 co-relative induction of vitellogenin (
VTG) mRNA in the liver and reduced
VTG receptor mRNA expression in the gonad of both sexes. The present work will provide a basic tool allowing future studies to examine the receptor–ligand interactions and endocrine disrupting mechanisms and also expands our knowledge of estrogen action on reproductive development in medaka.
In many vertebrates, estrogens are necessary to promote the growth and differentiation of the female reproductive system during development, and have important reproductive roles in both males and females. Medaka (
Oryzias latipes) has three estrogen receptor (ER) subtypes,
ERα,
ERβ1 and
ERβ2. To evaluate the three medaka ER (mER)-ligand interactions, we applied the ERE-luciferase reporter assay system to characterize each ER subtype. In this transient transfection assay system using mammalian cells, the mER proteins displayed estrogen-dependent activation. 17β-Estradiol (E
2) and
op’-DDT showed high activation irrespective of ERs. Endosulfan also exhibited activation; with less/no transactivity measured using other pesticides, i.e., heptachlor, carbendazim, deltamethrin, acephate, dimethoate and amitraz. It was generally observed that
ERβ2 had higher activation potential than
ERα and
ERβ1. To understand the molecular mechanism of estrogen action via ER, we also conducted E
2 treatment where we observed a trigger in
ERβ2 expression upon E
2 exposure. The present data suggest that
ERβ2 is essential for female gonad maintenance. The data were supported by induction of vitellogenin (
VTG) mRNA in the liver and reduced
VTG receptor mRNA expression in the gonad of both sexes. The present work will provide a basic tool allowing future studies to examine the receptor–ligand interactions and endocrine disrupting mechanisms, and also expands our knowledge of estrogen action on reproductive development in medaka.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>21145966</pmid><doi>10.1016/j.jsbmb.2010.11.015</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0960-0760 |
| ispartof | The Journal of steroid biochemistry and molecular biology, 2011-02, Vol.123 (3), p.115-121 |
| issn | 0960-0760 1879-1220 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_856789208 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Brain Environmental Exposure - adverse effects Environmental Pollutants - pharmacology Estrogen receptors Estrogenic contaminants Estrogens - pharmacology Female Fish Proteins - genetics Fish Proteins - metabolism Freshwater Fundamental and applied biological sciences. Psychology Gonads In Situ Hybridization, Fluorescence Liver Male Medaka Oryzias - metabolism Oryzias latipes Pesticides - pharmacology Receptors, Estrogen - genetics Receptors, Estrogen - metabolism RNA, Messenger - metabolism Vertebrates: endocrinology Vertebrates: reproduction Vitellogenins - genetics Vitellogenins - metabolism |
| title | Estrogen receptors in medaka ( Oryzias latipes) and estrogenic environmental contaminants: An in vitro– in vivo correlation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T13%3A46%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Estrogen%20receptors%20in%20medaka%20(%20Oryzias%20latipes)%20and%20estrogenic%20environmental%20contaminants:%20An%20in%20vitro%E2%80%93%20in%20vivo%20correlation&rft.jtitle=The%20Journal%20of%20steroid%20biochemistry%20and%20molecular%20biology&rft.au=Chakraborty,%20Tapas&rft.date=2011-02-01&rft.volume=123&rft.issue=3&rft.spage=115&rft.epage=121&rft.pages=115-121&rft.issn=0960-0760&rft.eissn=1879-1220&rft_id=info:doi/10.1016/j.jsbmb.2010.11.015&rft_dat=%3Cproquest_cross%3E856789208%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=848320737&rft_id=info:pmid/21145966&rft_els_id=S0960076010003742&rfr_iscdi=true |