Effects of extracellular acidic-alkaline stresses on trigeminal ganglion neurons in the mouse embryo in vivo
Acidic-alkaline stresses caused by ischemia and hypoglycemia induce neuronal cell death resulting from intracellular pH disturbance. The effects of acidic-alkaline disturbance on the trigeminal ganglion (TG) neurons of the embryonic mouse were investigated by caspase-3-immunohistochemistry and Nissl...
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| Veröffentlicht in: | Archives of toxicology 2011-02, Vol.85 (2), p.149-154 |
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| creator | Mukai, Shingo Nakagawa, Hiroshi Ichikawa, Hiroyuki Miyazaki, Shuichi Nishimura, Kazuhiko Matsuo, Saburo |
| description | Acidic-alkaline stresses caused by ischemia and hypoglycemia induce neuronal cell death resulting from intracellular pH disturbance. The effects of acidic-alkaline disturbance on the trigeminal ganglion (TG) neurons of the embryonic mouse were investigated by caspase-3-immunohistochemistry and Nissl staining. TG neurons exhibited apoptosis in 3.08 ± 0.55% of neurons in intact embryos at day 16. Intraperitoneal injection of alkaline solution (pH 8.97; 0.005-0.1 M K₂HPO₄ or 0.01-0.04 M KOH) into the embryo at embryonic day 15 significantly increased the number of apoptotic neurons in the TG at embryonic day 16 with dependence on concentration (3.40-6.05 and 2.93-5.55%, respectively). On the other hand, acidic solutions (pH 4.4; 0.01-0.2 M KH₂PO₄) slightly, but not significantly, increased the number of apoptotic cells (3.64-5.15%, without dependence on concentration). Neutral solutions (pH 7.4; 0.01-0.2 M potassium phosphate buffer) had no effect on neuronal survival in the TG (2.89-3.48%). The results indicated that alkaline stress significantly increased apoptosis in the developing nervous system, but acidic stress did not. |
| doi_str_mv | 10.1007/s00204-010-0556-2 |
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The effects of acidic-alkaline disturbance on the trigeminal ganglion (TG) neurons of the embryonic mouse were investigated by caspase-3-immunohistochemistry and Nissl staining. TG neurons exhibited apoptosis in 3.08 ± 0.55% of neurons in intact embryos at day 16. Intraperitoneal injection of alkaline solution (pH 8.97; 0.005-0.1 M K₂HPO₄ or 0.01-0.04 M KOH) into the embryo at embryonic day 15 significantly increased the number of apoptotic neurons in the TG at embryonic day 16 with dependence on concentration (3.40-6.05 and 2.93-5.55%, respectively). On the other hand, acidic solutions (pH 4.4; 0.01-0.2 M KH₂PO₄) slightly, but not significantly, increased the number of apoptotic cells (3.64-5.15%, without dependence on concentration). Neutral solutions (pH 7.4; 0.01-0.2 M potassium phosphate buffer) had no effect on neuronal survival in the TG (2.89-3.48%). The results indicated that alkaline stress significantly increased apoptosis in the developing nervous system, but acidic stress did not.</description><identifier>ISSN: 0340-5761</identifier><identifier>EISSN: 1432-0738</identifier><identifier>DOI: 10.1007/s00204-010-0556-2</identifier><identifier>PMID: 20480362</identifier><identifier>CODEN: ARTODN</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Acid-Base Imbalance - chemically induced ; Acid-Base Imbalance - metabolism ; Acid-Base Imbalance - pathology ; Acidity ; Acidosis - chemically induced ; Acidosis - pathology ; Alkalinity ; Alkalosis - chemically induced ; Alkalosis - pathology ; Animals ; Apoptosis ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Caspase 3 - metabolism ; Embryo, Mammalian - pathology ; Embryology ; Environmental Health ; Female ; Hydrogen-Ion Concentration ; Medical sciences ; Mice ; Mice, Inbred C57BL ; Neurons ; Neurons - metabolism ; Neurons - pathology ; Occupational Medicine/Industrial Medicine ; Pharmacology/Toxicology ; Pregnancy ; Reproductive Toxicology ; Rodents ; Stress, Physiological ; Toxicology ; Trigeminal Ganglion - embryology ; Trigeminal Ganglion - metabolism ; Trigeminal Ganglion - pathology</subject><ispartof>Archives of toxicology, 2011-02, Vol.85 (2), p.149-154</ispartof><rights>Springer-Verlag 2010</rights><rights>2015 INIST-CNRS</rights><rights>Springer-Verlag 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-ee9a46473471940fd55b1bcc15a9aee9ffb141dbb17822542e1195509c192ab23</citedby><cites>FETCH-LOGICAL-c456t-ee9a46473471940fd55b1bcc15a9aee9ffb141dbb17822542e1195509c192ab23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00204-010-0556-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00204-010-0556-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23839676$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20480362$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mukai, Shingo</creatorcontrib><creatorcontrib>Nakagawa, Hiroshi</creatorcontrib><creatorcontrib>Ichikawa, Hiroyuki</creatorcontrib><creatorcontrib>Miyazaki, Shuichi</creatorcontrib><creatorcontrib>Nishimura, Kazuhiko</creatorcontrib><creatorcontrib>Matsuo, Saburo</creatorcontrib><title>Effects of extracellular acidic-alkaline stresses on trigeminal ganglion neurons in the mouse embryo in vivo</title><title>Archives of toxicology</title><addtitle>Arch Toxicol</addtitle><addtitle>Arch Toxicol</addtitle><description>Acidic-alkaline stresses caused by ischemia and hypoglycemia induce neuronal cell death resulting from intracellular pH disturbance. The effects of acidic-alkaline disturbance on the trigeminal ganglion (TG) neurons of the embryonic mouse were investigated by caspase-3-immunohistochemistry and Nissl staining. TG neurons exhibited apoptosis in 3.08 ± 0.55% of neurons in intact embryos at day 16. Intraperitoneal injection of alkaline solution (pH 8.97; 0.005-0.1 M K₂HPO₄ or 0.01-0.04 M KOH) into the embryo at embryonic day 15 significantly increased the number of apoptotic neurons in the TG at embryonic day 16 with dependence on concentration (3.40-6.05 and 2.93-5.55%, respectively). On the other hand, acidic solutions (pH 4.4; 0.01-0.2 M KH₂PO₄) slightly, but not significantly, increased the number of apoptotic cells (3.64-5.15%, without dependence on concentration). Neutral solutions (pH 7.4; 0.01-0.2 M potassium phosphate buffer) had no effect on neuronal survival in the TG (2.89-3.48%). 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The effects of acidic-alkaline disturbance on the trigeminal ganglion (TG) neurons of the embryonic mouse were investigated by caspase-3-immunohistochemistry and Nissl staining. TG neurons exhibited apoptosis in 3.08 ± 0.55% of neurons in intact embryos at day 16. Intraperitoneal injection of alkaline solution (pH 8.97; 0.005-0.1 M K₂HPO₄ or 0.01-0.04 M KOH) into the embryo at embryonic day 15 significantly increased the number of apoptotic neurons in the TG at embryonic day 16 with dependence on concentration (3.40-6.05 and 2.93-5.55%, respectively). On the other hand, acidic solutions (pH 4.4; 0.01-0.2 M KH₂PO₄) slightly, but not significantly, increased the number of apoptotic cells (3.64-5.15%, without dependence on concentration). Neutral solutions (pH 7.4; 0.01-0.2 M potassium phosphate buffer) had no effect on neuronal survival in the TG (2.89-3.48%). The results indicated that alkaline stress significantly increased apoptosis in the developing nervous system, but acidic stress did not.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>20480362</pmid><doi>10.1007/s00204-010-0556-2</doi><tpages>6</tpages></addata></record> |
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| subjects | Acid-Base Imbalance - chemically induced Acid-Base Imbalance - metabolism Acid-Base Imbalance - pathology Acidity Acidosis - chemically induced Acidosis - pathology Alkalinity Alkalosis - chemically induced Alkalosis - pathology Animals Apoptosis Biological and medical sciences Biomedical and Life Sciences Biomedicine Caspase 3 - metabolism Embryo, Mammalian - pathology Embryology Environmental Health Female Hydrogen-Ion Concentration Medical sciences Mice Mice, Inbred C57BL Neurons Neurons - metabolism Neurons - pathology Occupational Medicine/Industrial Medicine Pharmacology/Toxicology Pregnancy Reproductive Toxicology Rodents Stress, Physiological Toxicology Trigeminal Ganglion - embryology Trigeminal Ganglion - metabolism Trigeminal Ganglion - pathology |
| title | Effects of extracellular acidic-alkaline stresses on trigeminal ganglion neurons in the mouse embryo in vivo |
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