Diversity of Burkholderia cepacia Complex from the Moso Bamboo (Phyllostachys edulis) Rhizhosphere Soil
The purpose of this study was to determine the existence of Burkholderia cepacia complex (Bcc) at species level and the predominant species in the environment of moso bamboo plantations in Hangzhou, China. A total of 423 isolates were recovered from moso bamboo rhizhosphere soil samples of three sit...
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| Veröffentlicht in: | Current microbiology 2011-02, Vol.62 (2), p.650-658 |
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| creator | Lou, Miaomiao Fang, Yuan Zhang, Guoqing Xie, Guanlin Zhu, Bo Ibrahim, Muhammad |
| description | The purpose of this study was to determine the existence of Burkholderia cepacia complex (Bcc) at species level and the predominant species in the environment of moso bamboo plantations in Hangzhou, China. A total of 423 isolates were recovered from moso bamboo rhizhosphere soil samples of three sites on the selective medium during 2007-2008. Isolates were identified by Bcc-specific PCR assays, followed by recA-restriction fragment length polymorphism assays, species-specific PCR analysis, recA gene sequencing, multilocus sequence typing (MLST) scheme, and BOX-PCR fingerprinting for genomic diversity. Out of 423 isolates, 278 isolates were assigned to the following Bcc species, eight B. stabilis, 26 B. anthina, 193 B. pyrrocinia, and 51 B. arboris, which indicated B. pyrrocinia as the most dominant species followed by B. arboris. Moreover, false positives were observed in certain isolates of B. arboris while performing species-specific PCR test. Furthermore, the results of recA gene sequence similarity and MLST data demonstrated that nine isolates formed a single discrete cluster but were PCR negative to species-specific primers representing novel species may exist within the Bcc. In addition, BOX-PCR fingerprinting for all the Bcc isolates also showed the strain diversity. It is the first report of the existence of B. arboris and predominance of B. pyrrocinia in the moso bamboo environment. |
| doi_str_mv | 10.1007/s00284-010-9758-3 |
| format | Article |
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A total of 423 isolates were recovered from moso bamboo rhizhosphere soil samples of three sites on the selective medium during 2007-2008. Isolates were identified by Bcc-specific PCR assays, followed by recA-restriction fragment length polymorphism assays, species-specific PCR analysis, recA gene sequencing, multilocus sequence typing (MLST) scheme, and BOX-PCR fingerprinting for genomic diversity. Out of 423 isolates, 278 isolates were assigned to the following Bcc species, eight B. stabilis, 26 B. anthina, 193 B. pyrrocinia, and 51 B. arboris, which indicated B. pyrrocinia as the most dominant species followed by B. arboris. Moreover, false positives were observed in certain isolates of B. arboris while performing species-specific PCR test. Furthermore, the results of recA gene sequence similarity and MLST data demonstrated that nine isolates formed a single discrete cluster but were PCR negative to species-specific primers representing novel species may exist within the Bcc. In addition, BOX-PCR fingerprinting for all the Bcc isolates also showed the strain diversity. It is the first report of the existence of B. arboris and predominance of B. pyrrocinia in the moso bamboo environment.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s00284-010-9758-3</identifier><identifier>PMID: 20882285</identifier><language>eng</language><publisher>New York: New York : Springer-Verlag</publisher><subject>Bacterial Typing Techniques ; Bamboo ; Biomedical and Life Sciences ; Biotechnology ; Burkholderia cepacia ; Burkholderia cepacia complex - classification ; Burkholderia cepacia complex - genetics ; Burkholderia cepacia complex - isolation & purification ; China ; Cluster Analysis ; DNA Fingerprinting ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; Dominant species ; Genomics ; Life Sciences ; Microbiology ; Molecular Sequence Data ; Multilocus Sequence Typing ; Phyllostachys ; Phylogeny ; Poaceae - microbiology ; Polymerase chain reaction ; Rec A Recombinases - genetics ; Rhizosphere ; Sequence Analysis, DNA ; Sequence Homology ; Soil Microbiology ; Soil microorganisms</subject><ispartof>Current microbiology, 2011-02, Vol.62 (2), p.650-658</ispartof><rights>Springer Science+Business Media, LLC 2010</rights><rights>Springer Science+Business Media, LLC 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c492t-84f04140db1126d1fcaff6a74f0118892bcd68214c65481ab1cefad4185cda0b3</citedby><cites>FETCH-LOGICAL-c492t-84f04140db1126d1fcaff6a74f0118892bcd68214c65481ab1cefad4185cda0b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00284-010-9758-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00284-010-9758-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20882285$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lou, Miaomiao</creatorcontrib><creatorcontrib>Fang, Yuan</creatorcontrib><creatorcontrib>Zhang, Guoqing</creatorcontrib><creatorcontrib>Xie, Guanlin</creatorcontrib><creatorcontrib>Zhu, Bo</creatorcontrib><creatorcontrib>Ibrahim, Muhammad</creatorcontrib><title>Diversity of Burkholderia cepacia Complex from the Moso Bamboo (Phyllostachys edulis) Rhizhosphere Soil</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><addtitle>Curr Microbiol</addtitle><description>The purpose of this study was to determine the existence of Burkholderia cepacia complex (Bcc) at species level and the predominant species in the environment of moso bamboo plantations in Hangzhou, China. A total of 423 isolates were recovered from moso bamboo rhizhosphere soil samples of three sites on the selective medium during 2007-2008. Isolates were identified by Bcc-specific PCR assays, followed by recA-restriction fragment length polymorphism assays, species-specific PCR analysis, recA gene sequencing, multilocus sequence typing (MLST) scheme, and BOX-PCR fingerprinting for genomic diversity. Out of 423 isolates, 278 isolates were assigned to the following Bcc species, eight B. stabilis, 26 B. anthina, 193 B. pyrrocinia, and 51 B. arboris, which indicated B. pyrrocinia as the most dominant species followed by B. arboris. Moreover, false positives were observed in certain isolates of B. arboris while performing species-specific PCR test. Furthermore, the results of recA gene sequence similarity and MLST data demonstrated that nine isolates formed a single discrete cluster but were PCR negative to species-specific primers representing novel species may exist within the Bcc. In addition, BOX-PCR fingerprinting for all the Bcc isolates also showed the strain diversity. It is the first report of the existence of B. arboris and predominance of B. pyrrocinia in the moso bamboo environment.</description><subject>Bacterial Typing Techniques</subject><subject>Bamboo</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Burkholderia cepacia</subject><subject>Burkholderia cepacia complex - classification</subject><subject>Burkholderia cepacia complex - genetics</subject><subject>Burkholderia cepacia complex - isolation & purification</subject><subject>China</subject><subject>Cluster Analysis</subject><subject>DNA Fingerprinting</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>Dominant species</subject><subject>Genomics</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Multilocus Sequence Typing</subject><subject>Phyllostachys</subject><subject>Phylogeny</subject><subject>Poaceae - microbiology</subject><subject>Polymerase chain reaction</subject><subject>Rec A Recombinases - genetics</subject><subject>Rhizosphere</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology</subject><subject>Soil Microbiology</subject><subject>Soil microorganisms</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkU1v1DAQhi0EokvhB3ABiwtwCMzYTmIf6fIpFYEoPVuOY29SknWwE8Ty6_EqBSQOcBrJ88w7Yz2E3Ed4hgD18wTApCgAoVB1KQt-g2xQcFaAUniTbIALXsiqxBNyJ6UrAGQK8DY5YSAlY7LckN3L_puLqZ8PNHh6tsQvXRhaF3tDrZuMzXUbxmlw36mPYaRz5-j7kAI9M2MTAn3ysTsMQ0izsd0hUdcuQ5-e0k9d_6MLaepcdPQi9MNdcsubIbl71_WUXL5-9Xn7tjj_8Obd9sV5YYVicyGFB4EC2gaRVS16a7yvTJ2fEaVUrLFtJRkKW5VComnQOm9agbK0rYGGn5LHa-4Uw9fFpVmPfbJuGMzehSVpWVa1LGXN_08KLhBFLTP56C_yKixxn7-RoexBSXWEcIVsDClF5_UU-9HEg0bQR1t6taWzLX20pY8nPLgOXprRtb8nfunJAFuBlFv7nYt_Nv8r9eE65E3QZhf7pC8vGCAHVNk_5_wnXVyokw</recordid><startdate>20110201</startdate><enddate>20110201</enddate><creator>Lou, Miaomiao</creator><creator>Fang, Yuan</creator><creator>Zhang, Guoqing</creator><creator>Xie, Guanlin</creator><creator>Zhu, Bo</creator><creator>Ibrahim, Muhammad</creator><general>New York : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20110201</creationdate><title>Diversity of Burkholderia cepacia Complex from the Moso Bamboo (Phyllostachys edulis) Rhizhosphere Soil</title><author>Lou, Miaomiao ; Fang, Yuan ; Zhang, Guoqing ; Xie, Guanlin ; Zhu, Bo ; Ibrahim, Muhammad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c492t-84f04140db1126d1fcaff6a74f0118892bcd68214c65481ab1cefad4185cda0b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Bacterial Typing Techniques</topic><topic>Bamboo</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Burkholderia cepacia</topic><topic>Burkholderia cepacia complex - classification</topic><topic>Burkholderia cepacia complex - genetics</topic><topic>Burkholderia cepacia complex - isolation & purification</topic><topic>China</topic><topic>Cluster Analysis</topic><topic>DNA Fingerprinting</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>Dominant species</topic><topic>Genomics</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Multilocus Sequence Typing</topic><topic>Phyllostachys</topic><topic>Phylogeny</topic><topic>Poaceae - microbiology</topic><topic>Polymerase chain reaction</topic><topic>Rec A Recombinases - genetics</topic><topic>Rhizosphere</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology</topic><topic>Soil Microbiology</topic><topic>Soil microorganisms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lou, Miaomiao</creatorcontrib><creatorcontrib>Fang, Yuan</creatorcontrib><creatorcontrib>Zhang, Guoqing</creatorcontrib><creatorcontrib>Xie, Guanlin</creatorcontrib><creatorcontrib>Zhu, Bo</creatorcontrib><creatorcontrib>Ibrahim, Muhammad</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Current microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lou, Miaomiao</au><au>Fang, Yuan</au><au>Zhang, Guoqing</au><au>Xie, Guanlin</au><au>Zhu, Bo</au><au>Ibrahim, Muhammad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diversity of Burkholderia cepacia Complex from the Moso Bamboo (Phyllostachys edulis) Rhizhosphere Soil</atitle><jtitle>Current microbiology</jtitle><stitle>Curr Microbiol</stitle><addtitle>Curr Microbiol</addtitle><date>2011-02-01</date><risdate>2011</risdate><volume>62</volume><issue>2</issue><spage>650</spage><epage>658</epage><pages>650-658</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><abstract>The purpose of this study was to determine the existence of Burkholderia cepacia complex (Bcc) at species level and the predominant species in the environment of moso bamboo plantations in Hangzhou, China. A total of 423 isolates were recovered from moso bamboo rhizhosphere soil samples of three sites on the selective medium during 2007-2008. Isolates were identified by Bcc-specific PCR assays, followed by recA-restriction fragment length polymorphism assays, species-specific PCR analysis, recA gene sequencing, multilocus sequence typing (MLST) scheme, and BOX-PCR fingerprinting for genomic diversity. Out of 423 isolates, 278 isolates were assigned to the following Bcc species, eight B. stabilis, 26 B. anthina, 193 B. pyrrocinia, and 51 B. arboris, which indicated B. pyrrocinia as the most dominant species followed by B. arboris. Moreover, false positives were observed in certain isolates of B. arboris while performing species-specific PCR test. Furthermore, the results of recA gene sequence similarity and MLST data demonstrated that nine isolates formed a single discrete cluster but were PCR negative to species-specific primers representing novel species may exist within the Bcc. In addition, BOX-PCR fingerprinting for all the Bcc isolates also showed the strain diversity. It is the first report of the existence of B. arboris and predominance of B. pyrrocinia in the moso bamboo environment.</abstract><cop>New York</cop><pub>New York : Springer-Verlag</pub><pmid>20882285</pmid><doi>10.1007/s00284-010-9758-3</doi><tpages>9</tpages></addata></record> |
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| subjects | Bacterial Typing Techniques Bamboo Biomedical and Life Sciences Biotechnology Burkholderia cepacia Burkholderia cepacia complex - classification Burkholderia cepacia complex - genetics Burkholderia cepacia complex - isolation & purification China Cluster Analysis DNA Fingerprinting DNA, Bacterial - chemistry DNA, Bacterial - genetics Dominant species Genomics Life Sciences Microbiology Molecular Sequence Data Multilocus Sequence Typing Phyllostachys Phylogeny Poaceae - microbiology Polymerase chain reaction Rec A Recombinases - genetics Rhizosphere Sequence Analysis, DNA Sequence Homology Soil Microbiology Soil microorganisms |
| title | Diversity of Burkholderia cepacia Complex from the Moso Bamboo (Phyllostachys edulis) Rhizhosphere Soil |
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